##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139367_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10343077
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04159468212409	33.0	31.0	34.0	30.0	34.0
2	32.22135550184921	33.0	31.0	34.0	30.0	34.0
3	32.23850349368955	34.0	31.0	34.0	30.0	34.0
4	35.988379666901835	37.0	35.0	37.0	35.0	37.0
5	35.85398996836241	37.0	35.0	37.0	35.0	37.0
6	35.87389052600111	37.0	35.0	37.0	35.0	37.0
7	35.815410733188976	37.0	35.0	37.0	35.0	37.0
8	35.8020540695965	37.0	35.0	37.0	33.0	37.0
9	37.57549982466533	39.0	37.0	39.0	35.0	39.0
10-11	37.453273286083046	39.0	37.0	39.0	34.0	39.0
12-13	37.43524552703224	39.0	37.0	39.0	34.0	39.0
14-15	38.68420625699683	40.0	38.0	41.0	34.0	41.0
16-17	38.64219424258371	40.0	38.0	41.0	34.0	41.0
18-19	38.6591144008693	40.0	38.0	41.0	34.0	41.0
20-21	38.64192066751069	40.0	38.0	41.0	34.0	41.0
22-23	38.56468592985014	40.0	38.0	41.0	34.0	41.0
24-25	38.50757905086945	40.0	38.0	41.0	34.0	41.0
26-27	38.34030144746194	40.0	38.0	41.0	34.0	41.0
28-29	38.23567175950812	40.0	38.0	41.0	33.5	41.0
30-31	38.08673075255817	40.0	37.5	41.0	33.0	41.0
32-33	37.91426049489672	40.0	37.0	41.0	33.0	41.0
34-35	38.0065328543793	40.0	37.0	41.0	33.0	41.0
36-37	37.981522127637135	40.0	37.0	41.0	33.0	41.0
38-39	37.947584477460765	40.0	37.0	41.0	33.0	41.0
40-41	37.87841013955344	40.0	37.0	41.0	33.0	41.0
42-43	37.781772016514	40.0	37.0	41.0	33.0	41.0
44-45	37.83949563339794	40.0	37.0	41.0	33.0	41.0
46-47	37.83978863035667	40.0	37.0	41.0	33.0	41.0
48-49	37.79153296092237	40.0	37.0	41.0	33.0	41.0
50-51	37.703208929052096	40.0	37.0	41.0	32.5	41.0
52-53	37.51968649332689	40.0	36.0	41.0	32.0	41.0
54-55	37.401963306321434	39.0	36.0	41.0	31.5	41.0
56-57	37.24320257067874	39.0	36.0	41.0	31.0	41.0
58-59	37.11171183968933	39.0	35.5	41.0	31.0	41.0
60-61	36.9023303286244	39.0	35.0	40.5	31.0	41.0
62-63	36.69733384844042	38.5	35.0	40.0	31.0	41.0
64-65	36.4026489611142	38.0	35.0	40.0	30.5	41.0
66-67	36.12867778507348	37.5	34.5	40.0	30.0	41.0
68-69	35.833887974740946	37.0	34.0	40.0	30.0	41.0
70-71	35.54680025096265	36.5	34.0	39.0	29.0	41.0
72-73	35.25824610176259	36.0	34.0	39.0	29.0	40.5
74-75	34.8913962268078	35.5	34.0	38.5	29.0	40.0
76-77	33.59698266585487	34.5	31.5	36.5	27.5	39.0
78-79	34.27065233790526	35.0	33.0	37.0	29.0	39.0
80-81	34.18602276938093	35.0	33.5	37.0	29.0	39.0
82-83	33.90180186969005	35.0	33.0	36.0	29.0	38.5
84-85	33.7182823149205	35.0	33.0	36.0	29.0	37.0
86-87	33.47335055877467	35.0	33.0	35.5	29.0	37.0
88-89	33.36728342187115	35.0	33.0	35.0	29.0	36.5
90-91	33.23183935575524	35.0	33.0	35.0	29.0	36.0
92-93	33.105611603004824	35.0	33.0	35.0	29.0	36.0
94-95	33.07078029368364	35.0	33.0	35.0	29.0	36.0
96-97	33.127127340736436	35.0	33.0	35.0	29.0	35.0
98-99	33.25836818425704	35.0	33.0	35.0	29.0	35.0
100	32.09628960041851	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	10.0
20	47.0
21	282.0
22	1567.0
23	5697.0
24	15596.0
25	33778.0
26	61194.0
27	98361.0
28	140815.0
29	187354.0
30	236406.0
31	293104.0
32	360358.0
33	450743.0
34	588793.0
35	799653.0
36	1208911.0
37	2032886.0
38	2571230.0
39	1211649.0
40	44641.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.848907921694874	20.657073325471714	14.857019821084188	23.636998931749226
2	14.458241005070349	21.577660110236057	41.496935583095826	22.467163301597772
3	17.76471353737384	26.596563092395037	31.057111921336368	24.58161144889475
4	12.411877045873293	18.700933967715798	39.52624542967243	29.360943556738484
5	12.917703310146486	38.210708476790806	35.98384697319762	12.88774123986508
6	29.27759311856617	38.70381125461988	19.15977228053122	12.858823346282735
7	26.114424169906115	33.00350562990104	23.509648047674787	17.372422152518055
8	24.190228884499266	36.911143560083715	21.435661747466444	17.462965807950575
9	25.506065554766728	17.043796541396723	21.35423530154518	36.09590260229137
10-11	23.805599629587984	27.00682785209856	29.40834241106394	19.779230107249514
12-13	24.797554189993665	24.527634500237912	29.321382279247377	21.353429030521045
14-15	21.855280590099056	25.990829421457462	26.983527242425055	25.170362746018426
16-17	21.455922620374146	29.09014335422254	27.62193027329355	21.832003752109763
18-19	21.44728401298472	28.36629386754714	29.221124585163288	20.96529753430485
20-21	22.329355232923774	27.547865531514077	29.085541607138254	21.037237628423892
22-23	22.035917089980224	27.449390935178393	28.919666606971496	21.59502536786989
24-25	21.798816468470857	27.585671795322636	28.97966502439614	21.635846711810366
26-27	21.775225272511168	27.787199171037795	28.838258037706748	21.599317518744286
28-29	21.711655817531234	27.89035808197263	28.8295973411059	21.56838875939024
30-31	22.03692331693099	27.699856761115182	28.757130755324102	21.506089166629724
32-33	21.80458258722084	27.804368151420217	28.708138848200175	21.68291041315877
34-35	21.953189209408634	27.869103231724157	28.67200334279381	21.505704216073397
36-37	21.923721771554654	27.8053301593979	28.63993309781998	21.631014971227465
38-39	21.74631508214508	27.9532634446253	28.703455291958317	21.596966181271295
40-41	22.061358614611017	27.795308657142968	28.55940969771895	21.583923030527068
42-43	21.904089420151575	27.86812265512795	28.394899589904366	21.83288833481611
44-45	22.009258743855646	27.9906812953647	28.2176328463552	21.782427114424454
46-47	22.046822411583204	27.94520825353084	27.9637714640585	22.04419787082746
48-49	21.946231834058487	28.063463099215546	28.0135947385884	21.976710328137568
50-51	21.9610872909081	28.1806687575622	27.9008183786332	21.957425572896494
52-53	22.105328009528865	28.05523382797116	27.841774159536975	21.997664002963
54-55	22.116027595936245	27.95707971120956	27.88531933574303	22.041573357111165
56-57	22.091476509993573	28.052963023239467	27.846328630032392	22.009231836734568
58-59	22.061465346676243	28.08993755717862	27.895667613595286	21.95292948254985
60-61	22.134801968164	28.07515555701821	27.86673223185721	21.92331024296058
62-63	22.056486484133437	28.141421262040645	27.84569599992138	21.956396253904543
64-65	22.05276065122784	28.116684765183987	27.79132608767997	22.039228495908205
66-67	22.106567136937755	28.152199656259576	27.639674424365158	22.101558782437515
68-69	22.048968918849347	28.167668679867326	27.70745187614515	22.07591052513818
70-71	22.031142637793977	27.968619317740202	27.78291141889338	22.217326625572444
72-73	22.082595563864544	27.983059607562446	27.731802048190946	22.202542780382068
74-75	22.019111179686828	28.10709610817877	27.68907644121973	22.18471627091467
76-77	22.078826798708505	28.025092767820592	27.660063320713498	22.236017112757402
78-79	22.084015314459133	28.06427959068694	27.601883719012566	22.249821375841353
80-81	22.08871240684296	28.080613746389975	27.61008703536033	22.220586811406733
82-83	22.081261757107328	28.072242828948557	27.715108869024945	22.131386544919167
84-85	22.23009675951042	28.03797871396806	27.641573831749643	22.090350694771875
86-87	22.01482930692821	28.101786248107206	27.71469568230018	22.168688762664406
88-89	22.11523536277613	28.08222613194224	27.66638686668018	22.13615163860145
90-91	22.149359229570255	28.117254931766837	27.660032041311243	22.073353797351665
92-93	22.142577298742918	28.086157447862014	27.713914353582787	22.057350899812278
94-95	21.9534122434393	28.199820131785565	27.79504918426096	22.051718440514183
96-97	21.961132348070727	28.32599074448946	27.893059991031382	21.819816916408428
98-99	23.044726033084512	30.286183309267013	28.087029907525736	18.582060750122743
100	22.09113289123444	31.37077839151909	21.80964103826566	24.728447678980814
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2643.0
1	2185.0
2	2513.0
3	3477.0
4	4471.5
5	6849.0
6	8964.5
7	10593.5
8	11821.5
9	11868.0
10	11217.5
11	10535.0
12	11310.0
13	14732.5
14	22750.5
15	34558.5
16	45159.5
17	51934.0
18	54638.5
19	53439.0
20	49903.0
21	46269.0
22	44674.5
23	47419.0
24	55186.0
25	68000.5
26	85720.5
27	106145.0
28	127974.5
29	152298.5
30	176124.5
31	199812.5
32	227447.5
33	254166.5
34	279944.5
35	304081.5
36	327524.5
37	350228.0
38	366907.5
39	380643.5
40	395757.0
41	410190.0
42	419174.0
43	427022.5
44	434220.0
45	439104.5
46	440990.0
47	437147.5
48	430961.5
49	420680.5
50	407423.0
51	390731.5
52	368986.0
53	344997.0
54	320863.0
55	295245.0
56	267703.0
57	239932.5
58	211905.0
59	183031.0
60	151762.5
61	122523.0
62	96630.5
63	73964.5
64	55754.0
65	41845.0
66	31557.5
67	24276.5
68	18507.5
69	13463.5
70	9610.5
71	6943.5
72	4947.0
73	3367.0
74	2297.0
75	1569.0
76	1044.0
77	667.5
78	438.5
79	276.0
80	185.5
81	143.0
82	111.0
83	78.5
84	53.0
85	33.5
86	19.5
87	13.5
88	9.5
89	7.0
90	7.0
91	7.5
92	7.0
93	4.0
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	4.8341513845444635E-6
14-15	0.0
16-17	9.281570658325371E-4
18-19	0.001469582020901517
20-21	0.0
22-23	0.0033021428296192445
24-25	2.5629432259718026E-4
26-27	0.0017269747702530966
28-29	0.005338529308259702
30-31	0.0015745612908883285
32-33	0.005722167092909065
34-35	0.010999687717844047
36-37	7.566074775077681E-4
38-39	6.374883351894051E-5
40-41	0.002696690677402289
42-43	0.005713218453338528
44-45	0.0016838702072844223
46-47	0.0019545142226171553
48-49	0.0029903875034498614
50-51	0.010534000711709814
52-53	0.006438426257168345
54-55	0.00977466015443963
56-57	0.017988112090771103
58-59	0.012421933170314954
60-61	0.01644688649152675
62-63	0.014275201097155748
64-65	0.010994676896490233
66-67	0.01334477750747256
68-69	0.008628902954500188
70-71	0.008878823761769423
72-73	0.006466447131624894
74-75	0.01074421400939419
76-77	0.011347102861230973
78-79	0.006518032493889057
80-81	0.003970630145408318
82-83	0.002143700110017752
84-85	7.148435901375175E-5
86-87	0.004244551268331005
88-89	5.375649468559018E-4
90-91	5.145528885666286E-4
92-93	0.001462227143372884
94-95	0.0011244046438040293
96-97	4.4228953355485655E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	182.0
20-21	754.0
22-23	1738.0
24-25	3373.0
26-27	4782.0
28-29	7971.0
30-31	17170.0
32-33	22550.0
34-35	31294.0
36-37	44206.0
38-39	52744.0
40-41	58438.0
42-43	71173.0
44-45	80909.0
46-47	79000.0
48-49	72166.0
50-51	64317.0
52-53	61460.0
54-55	66965.0
56-57	72100.0
58-59	74167.0
60-61	74695.0
62-63	75266.0
64-65	78906.0
66-67	84553.0
68-69	87378.0
70-71	87494.0
72-73	85228.0
74-75	87937.0
76-77	87065.0
78-79	94742.0
80-81	98368.0
82-83	96039.0
84-85	99079.0
86-87	105609.0
88-89	116906.0
90-91	128115.0
92-93	144748.0
94-95	179896.0
96-97	411160.0
98-99	733134.0
100-101	6499300.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.56751058501443
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.49236551120269	75.68146567646615
2	7.2286468280305	12.226173342896006
3	1.834138166139705	4.653254963381953
4	0.6645639453954176	2.2480207394658374
5	0.3053778261560936	1.2912521272942072
6	0.15450665007294945	0.7839745659299563
7	0.09109589838037763	0.5392627345373788
8	0.05893345831594073	0.39870846879558597
9	0.04501557431044236	0.34261695512899537
>10	0.12260685451829496	1.565524530606123
>50	0.002072273077786605	0.1160884514387167
>100	5.839379826123463E-4	0.0878236367655014
>500	7.446223474062641E-5	0.04200109952011868
>1k	1.8614182444454026E-5	0.023832707773467722
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.668302769088927E-6	0.0	0.0	0.0
3	0.0	9.668302769088927E-6	0.0	9.668302769088927E-6	0.0
4	0.0	3.867321107635571E-5	0.0	9.668302769088927E-6	0.0
5	0.0	4.834151384544464E-5	0.0	1.9336605538177854E-5	0.0
6	0.0	4.834151384544464E-5	0.0	1.9336605538177854E-5	1.9336605538177854E-5
7	0.0	4.834151384544464E-5	0.0	1.9336605538177854E-5	1.9336605538177854E-5
8	0.0	4.834151384544464E-5	0.0	1.9336605538177854E-5	2.9004908307266785E-5
9	0.0	1.1601963322906714E-4	0.0	4.834151384544464E-5	2.9004908307266785E-5
10-11	0.0	1.1601963322906714E-4	0.0	9.668302769088928E-5	2.9004908307266785E-5
12-13	0.0	1.2568793599815605E-4	0.0	1.450245415363339E-4	2.9004908307266785E-5
14-15	4.8341513845444635E-6	1.8853190399723409E-4	0.0	1.691952984590562E-4	2.9004908307266785E-5
16-17	9.668302769088927E-6	1.9336605538177855E-4	0.0	2.127026609199564E-4	3.3839059691811246E-5
18-19	9.668302769088927E-6	2.0786850953541196E-4	0.0	2.513758719963121E-4	4.350736246090018E-5
20-21	1.9336605538177854E-5	2.2237096368904534E-4	0.0	2.513758719963121E-4	4.834151384544464E-5
22-23	1.9336605538177854E-5	2.3687341784267872E-4	0.0	2.658783261499455E-4	4.834151384544464E-5
24-25	2.900490830726678E-5	2.4170756922722319E-4	0.0	3.0938568861084567E-4	4.834151384544464E-5
26-27	3.867321107635571E-5	2.4170756922722319E-4	0.0	3.673955052253793E-4	4.834151384544464E-5
28-29	3.867321107635571E-5	2.4170756922722319E-4	0.0	6.76781193836225E-4	5.800981661453357E-5
30-31	4.834151384544464E-5	2.803807803035789E-4	0.0	0.0016242748652069397	6.284396799907804E-5
32-33	5.31756652299891E-5	2.803807803035789E-4	0.0	0.003988174892249183	6.767811938362249E-5
34-35	5.800981661453357E-5	2.948832344572123E-4	0.0	0.006932173085436762	7.734642215271142E-5
36-37	5.800981661453357E-5	3.190539913799346E-4	0.0	0.012438271512432906	7.734642215271142E-5
38-39	5.800981661453357E-5	3.383905969181125E-4	0.0	0.028163765966356048	9.668302769088928E-5
40-41	5.800981661453357E-5	4.06068716301735E-4	0.0	0.04558121340486975	9.668302769088928E-5
42-43	6.767811938362249E-5	4.302394732244573E-4	0.0	0.056917298401626515	1.0151717907543373E-4
44-45	6.767811938362249E-5	4.979175926080798E-4	0.0	0.0703755758561983	1.063513304599782E-4
46-47	7.734642215271142E-5	5.414249550689799E-4	0.0	0.08583519198397149	1.063513304599782E-4
48-49	8.701472492180035E-5	5.559274092226134E-4	0.0	0.09998958723791769	1.063513304599782E-4
50-51	8.701472492180035E-5	6.912836479898584E-4	0.0	0.11405213361555754	1.063513304599782E-4
52-53	8.701472492180035E-5	7.29956859066214E-4	0.0	0.15154581175408438	1.3052208738270052E-4
54-55	9.184887630634482E-5	7.39625161835303E-4	0.0	0.19820504091770758	1.595269956899673E-4
56-57	9.668302769088928E-5	7.541276159889363E-4	0.0	0.24419232303887906	1.6436114707451177E-4
58-59	9.668302769088928E-5	7.589617673734808E-4	0.0	0.2747296573350464	1.6436114707451177E-4
60-61	9.668302769088928E-5	7.734642215271142E-4	0.0	0.2952071225999768	1.6436114707451177E-4
62-63	9.668302769088928E-5	7.734642215271142E-4	0.0	0.3115610567338907	1.6436114707451177E-4
64-65	9.668302769088928E-5	8.508106436798256E-4	0.0	0.31852706887901927	1.6436114707451177E-4
66-67	9.668302769088928E-5	8.508106436798256E-4	0.0	0.32451658244446985	1.6919529845905624E-4
68-69	9.668302769088928E-5	8.74981400602548E-4	0.0	0.3306124473403804	1.740294498436007E-4
70-71	9.668302769088928E-5	8.846497033716369E-4	0.0	0.3356109598719994	1.740294498436007E-4
72-73	9.668302769088928E-5	8.894838547561814E-4	0.0	0.33913505623133233	1.8853190399723409E-4
74-75	9.668302769088928E-5	8.943180061407258E-4	0.0	0.34185668346083087	1.9336605538177855E-4
76-77	9.668302769088928E-5	9.474936713707149E-4	0.0	0.34501821846632297	1.9336605538177855E-4
78-79	9.668302769088928E-5	9.668302769088927E-4	0.0	0.3470678986533698	1.9820020676632302E-4
80-81	1.1601963322906714E-4	9.716644282934373E-4	0.0	0.347797855512436	2.0786850953541196E-4
82-83	1.1601963322906714E-4	9.764985796779817E-4	0.0	0.3482184266828914	2.2237096368904534E-4
84-85	1.1601963322906714E-4	9.764985796779817E-4	0.0	0.34839729028411953	2.2237096368904534E-4
86-87	1.1601963322906714E-4	0.0010151717907543375	0.0	0.34847947085765674	2.2237096368904534E-4
88	1.2568793599815605E-4	0.0010538450018306932	0.0	0.3484843050090413	2.2237096368904534E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11965	0.0	48.29925	1
GTATCAA	21730	0.0	36.641445	1
TCAACGC	25790	0.0	30.225811	4
ATCAACG	25930	0.0	30.01309	3
CAACGCA	26380	0.0	29.53357	5
TATCAAC	27380	0.0	28.705069	2
AACGCAG	27395	0.0	28.454962	6
ACGCAGA	31230	0.0	25.001854	7
CGCAGAG	31605	0.0	24.63748	8
GCAGAGT	38060	0.0	20.425203	9
TACATGG	28415	0.0	20.33787	2
GTACATG	29150	0.0	20.162823	1
ACATGGG	28615	0.0	19.65717	3
GTGGTAT	6560	0.0	19.511322	1
GAGTACT	21335	0.0	19.111828	12-13
TGGTATC	6315	0.0	18.709194	2
AGAGTAC	33850	0.0	18.609045	10-11
CAGAGTA	37045	0.0	18.020975	10-11
CATGGGG	20625	0.0	17.579592	4
GTACTTT	24225	0.0	16.903116	14-15
>>END_MODULE