FastQCFastQC Report
Fri 27 May 2016
SRR2139366_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139366_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8143776
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101750.050.8175621
GTATCAA184750.038.265761
TCAACGC225150.030.886634
ATCAACG227600.030.516573
CAACGCA231050.030.097925
TATCAAC237000.029.7032132
AACGCAG239150.029.1142756
ACGCAGA273450.025.4779857
CGCAGAG276600.025.2187588
GCAGAGT328100.021.0387179
GTACATG242300.020.4927731
TACATGG239500.020.0894932
GAGTACT189900.019.30126812-13
GTGGTAT48450.019.1552521
ACATGGG245200.019.0643333
CAGAGTA316050.018.80289310-11
AGAGTAC293650.018.7297910-11
TGGTATC46900.018.511652
GTACTTT207200.017.63925714-15
AGTACTT206100.016.89181712-13
Produced by FastQC (version 0.11.5)