##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139366_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8143776
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0608609568829	33.0	31.0	34.0	30.0	34.0
2	32.24073132659837	33.0	31.0	34.0	30.0	34.0
3	32.254493492944796	34.0	31.0	34.0	30.0	34.0
4	35.99655933562023	37.0	35.0	37.0	35.0	37.0
5	35.867931534462635	37.0	35.0	37.0	35.0	37.0
6	35.89121103036233	37.0	35.0	37.0	35.0	37.0
7	35.83528611297757	37.0	35.0	37.0	35.0	37.0
8	35.82675542647539	37.0	35.0	37.0	35.0	37.0
9	37.60252209785731	39.0	37.0	39.0	35.0	39.0
10-11	37.48453862188744	39.0	37.0	39.0	34.5	39.0
12-13	37.45962603833897	39.0	37.0	39.0	34.0	39.0
14-15	38.70763648214293	40.0	38.0	41.0	34.0	41.0
16-17	38.66843587053475	40.0	38.0	41.0	34.0	41.0
18-19	38.69550310568464	40.0	38.0	41.0	34.0	41.0
20-21	38.680886440553394	40.0	38.0	41.0	34.0	41.0
22-23	38.60244474840039	40.0	38.0	41.0	34.0	41.0
24-25	38.54040351880307	40.0	38.0	41.0	34.0	41.0
26-27	38.37205372644928	40.0	38.0	41.0	34.0	41.0
28-29	38.26564564146173	40.0	38.0	41.0	34.0	41.0
30-31	38.11421454744739	40.0	37.5	41.0	33.0	41.0
32-33	37.93936698668908	40.0	37.0	41.0	33.0	41.0
34-35	38.02800235077818	40.0	37.0	41.0	33.0	41.0
36-37	38.00400424823812	40.0	37.0	41.0	33.0	41.0
38-39	37.96930819459817	40.0	37.0	41.0	33.0	41.0
40-41	37.897771070553105	40.0	37.0	41.0	33.0	41.0
42-43	37.79933453508653	40.0	37.0	41.0	33.0	41.0
44-45	37.85182553626446	40.0	37.0	41.0	33.0	41.0
46-47	37.8493027512933	40.0	37.0	41.0	33.0	41.0
48-49	37.796151983545485	40.0	37.0	41.0	33.0	41.0
50-51	37.71061164577653	40.0	37.0	41.0	32.5	41.0
52-53	37.52868709370976	40.0	36.0	41.0	32.0	41.0
54-55	37.408821253615514	39.0	36.0	41.0	31.5	41.0
56-57	37.24757902187012	39.0	36.0	41.0	31.0	41.0
58-59	37.115329936668594	39.0	35.0	41.0	31.0	41.0
60-61	36.9039965206383	39.0	35.0	40.5	31.0	41.0
62-63	36.69982597634389	38.5	35.0	40.0	31.0	41.0
64-65	36.40476342593857	38.0	35.0	40.0	30.5	41.0
66-67	36.129382324559714	37.5	34.5	40.0	30.0	41.0
68-69	35.83163457054902	37.0	34.0	40.0	30.0	41.0
70-71	35.545133076936025	36.5	34.0	39.0	29.0	41.0
72-73	35.26233129028773	36.0	34.0	39.0	29.0	40.5
74-75	34.897940401900414	35.5	34.0	38.5	29.0	40.0
76-77	33.60552225832282	34.5	32.0	36.5	28.0	39.0
78-79	34.28434400038161	35.0	33.5	37.0	29.0	39.0
80-81	34.20275473773068	35.0	34.0	37.0	29.0	39.0
82-83	33.92259700744765	35.0	33.0	36.0	29.0	38.5
84-85	33.736711494729704	35.0	33.0	36.0	29.0	37.0
86-87	33.49760071272049	35.0	33.0	35.5	29.0	37.0
88-89	33.38999570226321	35.0	33.0	35.0	29.0	36.5
90-91	33.257078564127305	35.0	33.0	35.0	29.0	36.0
92-93	33.132203472310415	35.0	33.0	35.0	29.0	36.0
94-95	33.09630779387057	35.0	33.0	35.0	29.0	36.0
96-97	33.149821959857064	35.0	33.0	35.0	29.0	35.0
98-99	33.27926108832859	35.0	33.0	35.0	29.0	35.0
100	32.08687687023272	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	7.0
20	43.0
21	266.0
22	1232.0
23	4556.0
24	12398.0
25	26564.0
26	48595.0
27	76353.0
28	109913.0
29	146350.0
30	184118.0
31	227098.0
32	278680.0
33	348495.0
34	458323.0
35	626263.0
36	951616.0
37	1617536.0
38	2031546.0
39	958419.0
40	35405.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.950254525664754	20.58891354575568	14.873370780335804	23.58746114824376
2	14.434041407818683	21.539627317843713	41.41900513963056	22.607326134707044
3	17.69871862880315	26.631282589305012	31.147516827574822	24.522481954317016
4	12.437264973889262	18.676090796210502	39.37352893792757	29.513115291972667
5	12.909674824061959	38.19092028071499	35.890058862129806	13.009346033093246
6	29.293217298707624	38.62054899348902	19.063601454656904	13.022632253146451
7	26.030529326936303	33.15918807197055	23.47864184869525	17.331640752397906
8	24.35699361082623	36.71495876114471	21.346166692207643	17.581880935821417
9	25.601490021336538	16.940262109370398	21.347984030994958	36.11026383829811
10-11	23.859085760708545	26.940911685193697	29.321263256749695	19.878739297348062
12-13	24.817434175182886	24.577201604906815	29.27904442350139	21.326319796408914
14-15	21.94369049443403	25.96053108533437	27.01766969032547	25.07810872990613
16-17	21.390143846092638	29.0980001237766	27.628809937099874	21.883046093030885
18-19	21.414829339657608	28.27509150845386	29.396425790338515	20.913653361550015
20-21	22.343912591689893	27.42435606783437	29.23693452014752	20.99479682032821
22-23	21.979861939072215	27.352633567217506	29.123350689199327	21.544153804510955
24-25	21.7739047355009	27.510032773224285	29.13731324418192	21.57874924709289
26-27	21.745684224651647	27.65738541152021	29.033675406899107	21.563254956929036
28-29	21.658227659787144	27.765025245710284	29.08446355124836	21.492283543254217
30-31	21.970099920449577	27.57925998416868	28.952913878791936	21.497726216589808
32-33	21.738608055717716	27.728367496271982	28.898474087599563	21.63455036041073
34-35	21.861561748735593	27.782299294408574	28.87277787026803	21.483361086587806
36-37	21.87465881603857	27.742703661831943	28.795228864883864	21.587408657245625
38-39	21.709738898584316	27.87341034896934	28.87176456916205	21.545086183284294
40-41	21.96859391720711	27.700905527884025	28.73448786015157	21.5960126947573
42-43	21.87475713517184	27.810912010411272	28.535415974154205	21.778914880262683
44-45	21.961257192945002	27.900966742469947	28.34634326078312	21.791432803801932
46-47	22.01079438028806	27.878597390910187	28.107377132468553	22.003231096333202
48-49	21.92372978042933	28.034764823933735	28.108314281138867	21.933191114498072
50-51	21.960597905879432	28.1193335339415	27.98016811587876	21.93990044430031
52-53	22.077106328269085	28.002136953312895	27.897368443076125	22.023388275341897
54-55	22.104729636132582	27.89427996820468	27.951690013133106	22.049300382529633
56-57	22.040893617974593	28.016008386367858	27.932852585962458	22.010245409695095
58-59	22.035448346790083	28.038166409552623	27.934025203854706	21.992360039802588
60-61	22.060210111405258	28.007488623603983	27.98781537188335	21.94448589310741
62-63	22.024958134497066	28.064945523294366	27.937395471905347	21.97270087030322
64-65	22.019306469049123	28.043614920140815	27.861637632837365	22.075440977972693
66-67	22.10729702202294	28.103076986332137	27.687523645086525	22.102102346558393
68-69	22.038466930071124	28.07546477107566	27.784724969548925	22.101343329304296
70-71	22.03107176995338	27.84812075979281	27.880060427995772	22.24074704225804
72-73	22.07943168747071	27.85224868288388	27.82491941095443	22.24340021869098
74-75	22.011649472885303	28.025574399269377	27.737743729953895	22.225032397891425
76-77	22.098590969781824	27.907204169718774	27.72067311704327	22.27353174345613
78-79	22.06941444437596	27.976514759643266	27.68171574053997	22.27235505544081
80-81	22.1012200047161	27.966067751222244	27.66011335675097	22.272598887310686
82-83	22.126499050412587	27.90326380178772	27.777605916167403	22.19263123163229
84-85	22.219453673361656	27.88971707906172	27.73550941273774	22.15531983483888
86-87	22.044843342507388	27.920103612136522	27.807708262895996	22.227344782460097
88-89	22.105945396368703	27.88028395611842	27.76612874937087	22.24764189814201
90-91	22.165826737273182	27.85836645185832	27.830366172566396	22.14544063830211
92-93	22.168034072976933	27.7880121467007	27.89389166979857	22.150062110523795
94-95	22.02441405577912	27.886740227380802	27.918742382487526	22.170103334352554
96-97	22.064139371607418	27.987311526902804	28.038888633667753	21.90966046782203
98-99	23.086937278979054	29.985985010054232	28.2377756478787	18.68930206308801
100	22.129766494530724	31.07224453032935	21.964789804279704	24.833199170860222
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1462.0
1	1299.0
2	1834.5
3	2720.0
4	3637.5
5	5763.5
6	7679.5
7	9236.0
8	10688.5
9	11213.5
10	10618.5
11	9712.0
12	10332.5
13	13193.0
14	20070.5
15	30448.0
16	39457.0
17	45029.0
18	46884.5
19	45487.5
20	42399.5
21	39127.0
22	37374.0
23	39255.5
24	45513.0
25	55871.0
26	70450.5
27	87167.0
28	104241.0
29	122972.5
30	141347.0
31	159813.0
32	180364.0
33	200058.5
34	219097.5
35	237445.5
36	255624.0
37	272936.5
38	285448.0
39	294911.5
40	307039.5
41	318193.5
42	324674.0
43	329789.0
44	334058.0
45	337167.0
46	337207.5
47	334373.5
48	330486.0
49	322816.5
50	312748.5
51	302002.0
52	287856.5
53	270118.0
54	251760.0
55	233406.0
56	213978.0
57	192814.0
58	170809.0
59	148403.0
60	124803.0
61	101863.5
62	80591.0
63	62383.5
64	47120.0
65	35100.5
66	26684.0
67	20687.5
68	15987.0
69	11674.5
70	8363.0
71	6005.0
72	4249.0
73	2911.0
74	1935.0
75	1296.5
76	858.0
77	557.0
78	363.0
79	246.5
80	168.5
81	106.5
82	75.5
83	56.0
84	43.0
85	36.0
86	30.0
87	21.5
88	12.5
89	6.5
90	3.5
91	2.5
92	2.5
93	3.0
94	2.5
95	2.0
96	2.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	1.2279316130502607E-5
14-15	0.0
16-17	8.841107613961878E-4
18-19	0.0015901714389000875
20-21	0.0
22-23	0.0033158282165191614
24-25	3.2551399581204744E-4
26-27	0.001634346843421892
28-29	0.00516358673061482
30-31	0.0015075463772029943
32-33	0.005301236605901756
34-35	0.010862819276054267
36-37	6.509770856065867E-4
38-39	3.113939481582636E-5
40-41	0.0025793034065273277
42-43	0.00542224385801782
44-45	0.0018911326945620162
46-47	0.0017404208388777254
48-49	0.0030569429139128113
50-51	0.01039962018326586
52-53	0.006544334466355288
54-55	0.009705593488158715
56-57	0.017912471433981808
58-59	0.012279861447905842
60-61	0.0159756545560124
62-63	0.014432425862907146
64-65	0.010550177753059654
66-67	0.012898791666028626
68-69	0.008815428531837729
70-71	0.008817439968105337
72-73	0.006020739744436638
74-75	0.010403817993270573
76-77	0.011386152526349672
78-79	0.0063245652703188
80-81	0.003982986281721654
82-83	0.0021122302852327316
84-85	6.0625120776607794E-5
86-87	0.00392106038363931
88-89	5.83333825926342E-4
90-91	4.976047440214568E-4
92-93	0.0015023274426297704
94-95	0.001155210379786473
96-97	4.375200354727783E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	175.0
20-21	589.0
22-23	1259.0
24-25	3080.0
26-27	3451.0
28-29	6354.0
30-31	13484.0
32-33	18002.0
34-35	24752.0
36-37	34273.0
38-39	40756.0
40-41	45435.0
42-43	57244.0
44-45	64732.0
46-47	62763.0
48-49	56758.0
50-51	51018.0
52-53	48021.0
54-55	52449.0
56-57	56915.0
58-59	57338.0
60-61	58064.0
62-63	58899.0
64-65	62681.0
66-67	67401.0
68-69	69508.0
70-71	69218.0
72-73	68310.0
74-75	70825.0
76-77	70506.0
78-79	76132.0
80-81	78903.0
82-83	76694.0
84-85	80806.0
86-87	85410.0
88-89	95389.0
90-91	104285.0
92-93	117249.0
94-95	138364.0
96-97	321045.0
98-99	576943.0
100-101	5098296.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.67694136814178
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.97248736915978	77.0856752507338
2	7.061613861275983	12.100349535139996
3	1.6914627921744425	4.347580754145692
4	0.6047458175818226	2.072510878223471
5	0.26733066995544097	1.1452037067839187
6	0.14790567642775393	0.7603263580389614
7	0.06837285953858446	0.41005842345016463
8	0.04707509936685129	0.3226600422682533
9	0.0339025191017997	0.261419772718548
>10	0.10337230952271734	1.3235196620109209
>50	0.0013554678806257062	0.07450590212790396
>100	3.3124188530156374E-4	0.05438617176332627
>500	2.2158065161563056E-5	0.016307558794740058
>1k	2.2158063768582666E-5	0.025495983800364188
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.4558632261005213E-5	0.0	0.0	0.0
3	0.0	3.683794839150782E-5	0.0	1.2279316130502607E-5	0.0
4	0.0	6.139658065251303E-5	0.0	2.4558632261005213E-5	1.2279316130502607E-5
5	0.0	6.139658065251303E-5	0.0	2.4558632261005213E-5	1.2279316130502607E-5
6	3.683794839150782E-5	6.139658065251303E-5	0.0	2.4558632261005213E-5	2.4558632261005213E-5
7	3.683794839150782E-5	6.139658065251303E-5	0.0	2.4558632261005213E-5	2.4558632261005213E-5
8	3.683794839150782E-5	7.367589678301565E-5	0.0	3.683794839150782E-5	2.4558632261005213E-5
9	3.683794839150782E-5	8.595521291351825E-5	0.0	3.683794839150782E-5	2.4558632261005213E-5
10-11	3.683794839150782E-5	8.595521291351825E-5	0.0	4.2977606456759125E-5	2.4558632261005213E-5
12-13	3.683794839150782E-5	8.595521291351825E-5	0.0	6.139658065251303E-5	2.4558632261005213E-5
14-15	3.683794839150782E-5	1.596311096965339E-4	0.0	6.753623871776435E-5	6.139658065251304E-5
16-17	7.367589678301565E-5	1.719104258270365E-4	0.0	7.981555484826695E-5	7.981555484826695E-5
18-19	8.595521291351825E-5	1.841897419575391E-4	0.0	8.595521291351825E-5	8.595521291351825E-5
20-21	8.595521291351825E-5	2.087483742185443E-4	0.0	1.1665350323977477E-4	8.595521291351825E-5
22-23	8.595521291351825E-5	2.2716734841429824E-4	0.0	3.5610016778457566E-4	1.227931613050261E-4
24-25	8.595521291351825E-5	2.6400529680580605E-4	0.0	9.8848494850546E-4	1.903294000227904E-4
26-27	8.595521291351825E-5	2.701449548710574E-4	0.0	0.002609354677731804	1.964690580880417E-4
28-29	8.595521291351825E-5	2.701449548710574E-4	0.0	0.008920923168810144	1.964690580880417E-4
30-31	8.595521291351825E-5	2.947035871320626E-4	0.0	0.009921687433446107	2.02608716153293E-4
32-33	8.595521291351825E-5	2.947035871320626E-4	0.0	0.012580159375699921	2.087483742185443E-4
34-35	8.595521291351825E-5	3.43820851654073E-4	0.0	0.01587715575673987	2.2102769034904694E-4
36-37	8.595521291351825E-5	4.05217432306586E-4	0.0	0.021458104938053307	2.2102769034904694E-4
38-39	8.595521291351825E-5	4.05217432306586E-4	0.0	0.03704669676572637	2.3330700647954952E-4
40-41	8.595521291351825E-5	4.297760645675913E-4	0.0	0.05442192909038755	2.455863226100521E-4
42-43	8.595521291351825E-5	4.359157226328426E-4	0.0	0.06583555343368973	2.5172598067530345E-4
44-45	8.595521291351825E-5	4.973123032853556E-4	0.0	0.08074264321611989	2.701449548710574E-4
46-47	8.595521291351825E-5	5.525692258726173E-4	0.0	0.09679170939868681	3.192622193930678E-4
48-49	9.209487097876955E-5	5.587088839378686E-4	0.0	0.11198122345211853	3.561001677845756E-4
50-51	9.823452904402085E-5	5.894071742641252E-4	0.0	0.12662430793774287	3.561001677845756E-4
52-53	9.823452904402085E-5	5.894071742641252E-4	0.0	0.1645182775164739	3.7451914198032954E-4
54-55	9.823452904402085E-5	6.201054645903817E-4	0.0	0.2100254230961166	4.05217432306586E-4
56-57	9.823452904402085E-5	6.508037549166381E-4	0.0	0.25616495345648016	4.174967484370886E-4
58-59	9.823452904402085E-5	6.508037549166381E-4	0.0	0.28670975233110535	4.174967484370886E-4
60-61	1.1665350323977477E-4	6.630830710471407E-4	0.0	0.3077135225723301	4.174967484370886E-4
62-63	1.350724774355287E-4	6.999210194386487E-4	0.0	0.3245791632775754	4.297760645675913E-4
64-65	1.350724774355287E-4	8.227141807436747E-4	0.0	0.3321984789365523	4.297760645675913E-4
66-67	1.350724774355287E-4	8.349934968741772E-4	0.0	0.33865739922119664	4.4819503876334515E-4
68-69	1.350724774355287E-4	8.595521291351826E-4	0.0	0.3451470177961673	4.666140129590991E-4
70-71	1.350724774355287E-4	8.718314452656852E-4	0.0	0.35025521330645637	4.788933290896017E-4
72-73	1.719104258270365E-4	8.841107613961878E-4	0.0	0.3539390081456072	5.894071742641252E-4
74-75	1.719104258270365E-4	9.148090517224442E-4	0.0	0.3571316303395378	5.894071742641252E-4
76-77	1.780500838922878E-4	9.762056323749573E-4	0.0	0.3598330798882484	5.955468323293764E-4
78-79	1.841897419575391E-4	0.0010191832388317165	0.0	0.36176707217880255	6.016864903946277E-4
80-81	1.841897419575391E-4	0.0010376022130274703	0.0	0.36243629490791496	6.262451226556329E-4
82-83	1.841897419575391E-4	0.0010437418710927215	0.0	0.36293360721120027	6.630830710471408E-4
84-85	1.903294000227904E-4	0.0010560211872232242	0.0	0.3631177969531578	6.630830710471408E-4
86-87	1.964690580880417E-4	0.0011174177678757373	0.0	0.36320375216607137	6.692227291123921E-4
88	1.964690580880417E-4	0.0011910936646587528	0.0	0.3632098918241366	6.753623871776433E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10175	0.0	50.817562	1
GTATCAA	18475	0.0	38.26576	1
TCAACGC	22515	0.0	30.88663	4
ATCAACG	22760	0.0	30.51657	3
CAACGCA	23105	0.0	30.09792	5
TATCAAC	23700	0.0	29.703213	2
AACGCAG	23915	0.0	29.114275	6
ACGCAGA	27345	0.0	25.477985	7
CGCAGAG	27660	0.0	25.218758	8
GCAGAGT	32810	0.0	21.038717	9
GTACATG	24230	0.0	20.492773	1
TACATGG	23950	0.0	20.089493	2
GAGTACT	18990	0.0	19.301268	12-13
GTGGTAT	4845	0.0	19.155252	1
ACATGGG	24520	0.0	19.064333	3
CAGAGTA	31605	0.0	18.802893	10-11
AGAGTAC	29365	0.0	18.72979	10-11
TGGTATC	4690	0.0	18.51165	2
GTACTTT	20720	0.0	17.639257	14-15
AGTACTT	20610	0.0	16.891817	12-13
>>END_MODULE