FastQCFastQC Report
Fri 27 May 2016
SRR2139365_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139365_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8662006
Sequences flagged as poor quality0
Sequence length19-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107250.049.7788121
GTATCAA199350.038.763541
TCAACGC250700.030.1166934
ATCAACG252000.029.9613323
TATCAAC259150.029.362692
CAACGCA256950.029.3512925
AACGCAG264300.028.5989276
ACGCAGA309350.024.3931967
CGCAGAG314250.023.9859818
GTACATG291550.022.4662191
TACATGG299300.021.5460642
GCAGAGT363350.020.5821089
ACATGGG308950.020.3949623
GTGGTAT50100.019.7961831
GAGTACT185500.018.97556512-13
AGAGTAC323250.018.81408110-11
CATGGGG238650.018.2325954
CAGAGTA349950.018.14444710-11
TGGTATC52850.017.3287012
GTACTTT202750.017.22690814-15
Produced by FastQC (version 0.11.5)