##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139365_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8662006
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.99098176565567	31.0	31.0	34.0	30.0	34.0
2	32.17429865553083	33.0	31.0	34.0	30.0	34.0
3	32.18569555366274	34.0	31.0	34.0	30.0	34.0
4	35.94989532447796	37.0	35.0	37.0	35.0	37.0
5	35.806900156845884	37.0	35.0	37.0	35.0	37.0
6	35.831409144717746	37.0	35.0	37.0	35.0	37.0
7	35.77119318550461	37.0	35.0	37.0	33.0	37.0
8	35.76079443953283	37.0	35.0	37.0	33.0	37.0
9	37.5267446131993	39.0	37.0	39.0	35.0	39.0
10-11	37.39965199747033	39.0	37.0	39.0	34.0	39.0
12-13	37.37155896682593	39.0	37.0	39.0	34.0	39.0
14-15	38.59528075829087	40.0	38.0	41.0	34.0	41.0
16-17	38.548852713794005	40.0	38.0	41.0	34.0	41.0
18-19	38.563956951773065	40.0	38.0	41.0	34.0	41.0
20-21	38.544276351867815	40.0	38.0	41.0	34.0	41.0
22-23	38.45878632421872	40.0	38.0	41.0	34.0	41.0
24-25	38.38628429207233	40.0	38.0	41.0	34.0	41.0
26-27	38.19846314034089	40.0	38.0	41.0	33.0	41.0
28-29	38.087558790748496	40.0	37.5	41.0	33.0	41.0
30-31	37.930816564310774	40.0	37.0	41.0	33.0	41.0
32-33	37.74899726400609	40.0	37.0	41.0	33.0	41.0
34-35	37.84054572351887	40.0	37.0	41.0	33.0	41.0
36-37	37.810744450247896	40.0	37.0	41.0	33.0	41.0
38-39	37.771608793741436	40.0	37.0	41.0	33.0	41.0
40-41	37.687466772556476	40.0	37.0	41.0	32.5	41.0
42-43	37.57170594639315	40.0	37.0	41.0	32.0	41.0
44-45	37.620599783954304	40.0	37.0	41.0	32.0	41.0
46-47	37.60459399758922	40.0	37.0	41.0	32.0	41.0
48-49	37.53720570118118	40.0	36.0	41.0	32.0	41.0
50-51	37.42615902787806	39.5	36.0	41.0	31.5	41.0
52-53	37.20924447659418	39.0	36.0	41.0	31.0	41.0
54-55	37.058698296351196	39.0	35.0	41.0	31.0	41.0
56-57	36.86439678983696	39.0	35.0	40.5	31.0	41.0
58-59	36.697421589950245	38.5	35.0	40.0	31.0	41.0
60-61	36.44815840318802	38.0	35.0	40.0	30.0	41.0
62-63	36.20605870784269	38.0	35.0	40.0	30.0	41.0
64-65	35.87526601812965	37.0	34.0	40.0	29.5	41.0
66-67	35.567901594819084	36.5	34.0	39.5	29.0	41.0
68-69	35.2475715272255	36.0	34.0	39.0	29.0	41.0
70-71	34.94261980128621	36.0	34.0	39.0	29.0	40.0
72-73	34.6556693918757	35.0	33.5	38.0	29.0	40.0
74-75	34.30003257788559	35.0	33.0	37.0	29.0	39.5
76-77	33.06266229210202	34.0	31.5	36.0	27.0	39.0
78-79	33.76432395676251	35.0	33.0	36.0	28.5	39.0
80-81	33.718235215856126	35.0	33.0	36.0	29.0	38.5
82-83	33.47472546341194	35.0	33.0	36.0	29.0	37.0
84-85	33.33591254486402	35.0	33.0	35.0	29.0	37.0
86-87	33.13291350166009	35.0	33.0	35.0	29.0	36.5
88-89	33.06550182190956	35.0	33.0	35.0	29.0	36.0
90-91	32.96275025486945	35.0	33.0	35.0	29.0	36.0
92-93	32.85856007679483	35.0	32.0	35.0	29.0	35.5
94-95	32.84984247807707	34.0	32.0	35.0	29.0	35.0
96-97	32.930648272666105	34.0	32.0	35.0	29.0	35.0
98-99	33.08508128236799	34.0	33.0	35.0	29.0	35.0
100	31.829513668438334	34.0	31.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	8.0
20	52.0
21	340.0
22	1659.0
23	5718.0
24	15285.0
25	33299.0
26	59394.0
27	93505.0
28	132527.0
29	173696.0
30	216408.0
31	264193.0
32	322762.0
33	402484.0
34	529464.0
35	728757.0
36	1116149.0
37	1811703.0
38	1912680.0
39	814176.0
40	27745.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.92848631136945	19.768007549290544	14.44947048062539	23.85403565871462
2	15.21481282742127	20.916205784202873	40.27433137312535	23.594650015250508
3	18.291617438270073	26.034465919326312	30.36468688661726	25.30922975578636
4	13.24769343267599	17.891167473215788	37.6266305980393	31.234508496068926
5	13.71770003391824	37.61432397991874	34.92907993829605	13.738896047866971
6	30.832119026470313	37.038810640399	18.33929692498481	13.789773408145873
7	27.22675324861239	32.43049011972515	22.556691833277416	17.78606479838504
8	25.493217160089703	35.649097911038155	20.50738593346622	18.350298995405915
9	26.26072990482805	16.51679761016097	20.452641108768567	36.76983137624241
10-11	24.912133517339978	26.06817635545392	28.24312289785992	20.77656722934618
12-13	25.841509798163948	23.6962098442445	28.31307945948545	22.149200898106102
14-15	22.931177835711498	24.936862200280167	25.86403195749345	26.26792800651489
16-17	22.472084127326074	28.098488117758674	26.485998828434997	22.943428926480255
18-19	22.622682941288488	27.25404980367693	28.19989054327238	21.9233767117622
20-21	23.39041543461104	26.38190345609787	28.024558988352833	22.203122120938257
22-23	23.328460294451478	26.24125087263229	27.909638363628847	22.520650469287386
24-25	23.01572824560969	26.480067448589416	27.842429867654488	22.661774438146402
26-27	22.886031816871867	26.636433211735987	27.835554697808806	22.641980273583346
28-29	22.87623253439398	26.61423504941466	27.888501019585014	22.621031396606348
30-31	23.02768901880291	26.52258233594278	27.71485549740499	22.734873147849328
32-33	22.849054676828413	26.702701636254545	27.729901365150877	22.718342321766166
34-35	23.016770275946094	26.660501383753576	27.700817187212714	22.621911153087616
36-37	23.00422488935997	26.6355465939644	27.633970725789464	22.726257790886166
38-39	22.858605037450737	26.776814500907985	27.69519890649693	22.669381555144344
40-41	23.037314465001074	26.610338369189478	27.632026039758657	22.720321126050795
42-43	22.95193324538183	26.720630900783256	27.39018441156718	22.937251442267733
44-45	22.99654612503431	26.759525273729306	27.25625293585398	22.987675665382397
46-47	23.157600053068702	26.81895112627978	27.00444815546696	23.019000665184556
48-49	23.02653534769693	26.96774482106451	26.894572758710854	23.111147072527704
50-51	23.025120649411683	27.068182884470893	26.825027844794036	23.08166862132338
52-53	23.213515158410853	26.905259729205746	26.733872936848353	23.147352175535048
54-55	23.16121615079708	26.793368651231543	26.77033387154559	23.275081326425795
56-57	23.106199085357865	26.877031439388098	26.87151879554632	23.145250679707715
58-59	23.097091367533626	26.904943079623273	26.78361837558735	23.21434717725575
60-61	23.155874855862425	26.90681442050854	26.831687973791972	23.105622749837064
62-63	23.118234206404484	26.975295645087094	26.80977918855985	23.09669095994857
64-65	23.132206160475334	26.96608616069077	26.681987526563226	23.219720152270668
66-67	23.160194904482335	26.972871440769513	26.586944227115456	23.279989427632696
68-69	23.062736769174908	26.966382670360105	26.687780465079143	23.28310009538584
70-71	23.10630725015507	26.844297174770727	26.72819371647136	23.321201858602837
72-73	23.161116730046942	26.822420787079682	26.609232488619348	23.40722999425403
74-75	23.03539144598484	26.985860113337967	26.644715933805674	23.334032506871523
76-77	23.2943191051816	26.85336318630688	26.586149841949506	23.26616786656201
78-79	23.150730070294493	26.844933116029207	26.570404694232053	23.433932119444247
80-81	23.200910955382234	26.912908490222392	26.587949760659313	23.29823079373606
82-83	23.205593531723935	26.836325877236966	26.66835201668338	23.289728574355724
84-85	23.19123636275099	26.860160942771135	26.66004084146372	23.288561853014162
86-87	23.12764774580811	26.878320465396076	26.759230216268428	23.23480157252739
88-89	23.12302211626804	26.788337166499527	26.703899921335022	23.38474079589741
90-91	23.2064420552672	26.75630859814048	26.756011213387744	23.281238133204578
92-93	23.270622553840347	26.731465063376643	26.776352713871336	23.22155966891167
94-95	23.066415253192027	26.696848852712797	26.936234553783994	23.300501340311186
96-97	23.131289883026763	26.836255797232806	26.963523006637917	23.06893131310251
98-99	24.234430216950447	28.877609260216996	27.05594793569148	19.832012587141072
100	23.267211692045066	29.80024116561471	20.779692442236254	26.152854700103973
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1812.0
1	1594.5
2	2140.0
3	3040.5
4	3885.0
5	6175.0
6	8312.5
7	10239.0
8	11800.5
9	11877.0
10	10702.5
11	9361.0
12	9922.5
13	13001.0
14	20319.5
15	31083.0
16	40283.0
17	45604.0
18	46885.5
19	44533.0
20	40148.5
21	35600.0
22	31984.5
23	31720.0
24	35886.0
25	43357.5
26	53443.0
27	65054.5
28	78848.5
29	95330.0
30	112518.5
31	130707.5
32	150067.5
33	168135.0
34	185647.0
35	202197.0
36	219798.5
37	239495.0
38	257425.5
39	270658.5
40	283688.0
41	298102.5
42	311161.0
43	324525.5
44	336741.0
45	349359.0
46	359143.0
47	365346.5
48	370959.5
49	371312.0
50	370966.0
51	371095.0
52	366706.5
53	357601.0
54	345302.0
55	328725.5
56	309218.5
57	285613.5
58	256707.5
59	225183.0
60	190466.0
61	158533.0
62	129561.0
63	102176.5
64	78389.0
65	59221.5
66	45016.5
67	34548.5
68	26345.5
69	19249.0
70	13956.5
71	10262.0
72	7220.5
73	4734.5
74	3069.0
75	2039.5
76	1390.5
77	902.0
78	608.0
79	410.0
80	265.5
81	175.5
82	120.0
83	80.5
84	62.5
85	51.0
86	36.5
87	26.0
88	19.0
89	13.5
90	9.5
91	6.5
92	5.5
93	3.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	5.772334953358379E-6
14-15	0.0
16-17	0.0010159309517910747
18-19	0.0015643027723601208
20-21	0.0
22-23	0.003209844384896828
24-25	2.656176693493089E-4
26-27	0.0016173951308435116
28-29	0.005282455007671986
30-31	0.001539528434337781
32-33	0.005431062754537549
34-35	0.011363571533275297
36-37	6.549991818358434E-4
38-39	2.9421809657838477E-5
40-41	0.002749534801229374
42-43	0.005373287025947722
44-45	0.0018017796521106976
46-47	0.0017952966271244978
48-49	0.0028444753316102245
50-51	0.01049783080979486
52-53	0.005900927731993725
54-55	0.009342652834004084
56-57	0.01780349407683397
58-59	0.011725485467402607
60-61	0.016165062518137298
62-63	0.014229929095114512
64-65	0.010376595549333685
66-67	0.012471238578970985
68-69	0.008344978462789332
70-71	0.008587737441804374
72-73	0.0059760540744005055
74-75	0.010521040180633066
76-77	0.01188187378964755
78-79	0.006732370557297025
80-81	0.004112238407021647
82-83	0.0022324729738916983
84-85	6.580488588116691E-5
86-87	0.004309366296579474
88-89	5.254312476965469E-4
90-91	4.7276326928306065E-4
92-93	0.0012649713825446521
94-95	0.0011565540930251312
96-97	3.747692012389218E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	202.0
20-21	647.0
22-23	1432.0
24-25	2950.0
26-27	3536.0
28-29	9263.0
30-31	20983.0
32-33	26106.0
34-35	35731.0
36-37	50251.0
38-39	57935.0
40-41	61609.0
42-43	73711.0
44-45	81580.0
46-47	79076.0
48-49	71878.0
50-51	63408.0
52-53	58676.0
54-55	63193.0
56-57	66667.0
58-59	67556.0
60-61	67881.0
62-63	67269.0
64-65	70399.0
66-67	75305.0
68-69	76988.0
70-71	76649.0
72-73	74510.0
74-75	76272.0
76-77	74994.0
78-79	81227.0
80-81	83242.0
82-83	81501.0
84-85	84670.0
86-87	89194.0
88-89	101168.0
90-91	110952.0
92-93	127028.0
94-95	151057.0
96-97	352735.0
98-99	605510.0
100-101	5237065.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.17484606431866
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.15530472395459	72.44148595444071
2	7.8537289875154865	12.907579411599245
3	2.1046635307996318	5.188512049819353
4	0.8373642350731338	2.7524110846760284
5	0.3932095030880588	1.615596519364423
6	0.21555400329548036	1.062787022361224
7	0.12802722905957215	0.7364432488008124
8	0.0773645229692189	0.5085934212668006
9	0.05871209775297786	0.4342191835467766
>10	0.17310214979746116	2.1540142793511947
>50	0.0024707679748003576	0.1336873192847802
>100	4.982487195502783E-4	0.06467050548853112
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.3089339813433518E-5	0.0	0.0	0.0
3	0.0	2.3089339813433518E-5	0.0	0.0	0.0
4	0.0	6.926801944030055E-5	0.0	0.0	1.1544669906716759E-5
5	0.0	6.926801944030055E-5	0.0	0.0	1.1544669906716759E-5
6	3.463400972015028E-5	6.926801944030055E-5	0.0	0.0	2.3089339813433518E-5
7	3.463400972015028E-5	6.926801944030055E-5	0.0	0.0	2.3089339813433518E-5
8	3.463400972015028E-5	6.926801944030055E-5	0.0	1.1544669906716759E-5	3.463400972015028E-5
9	3.463400972015028E-5	1.8471471850746814E-4	0.0	2.3089339813433518E-5	4.6178679626867036E-5
10-11	6.349568448694217E-5	1.8471471850746814E-4	0.0	2.3089339813433518E-5	4.6178679626867036E-5
12-13	6.926801944030055E-5	1.8471471850746814E-4	0.0	3.463400972015028E-5	5.195101458022541E-5
14-15	7.504035439365894E-5	2.539827379477687E-4	0.0	5.195101458022541E-5	8.658502430037569E-5
16-17	1.2699136897388434E-4	2.7707207776120216E-4	0.0	6.926801944030055E-5	1.0390202916045082E-4
18-19	1.2699136897388434E-4	3.0016141757463575E-4	0.0	1.2121903402052596E-4	1.327637039272427E-4
20-21	1.616253786940346E-4	3.1747842243471085E-4	0.0	1.6739771364739298E-4	1.7894238355410977E-4
22-23	1.616253786940346E-4	3.4634009720150275E-4	0.0	5.772334953358379E-4	2.2512106318097678E-4
24-25	1.904870534608265E-4	3.7520177196829466E-4	0.0	0.0019625938841418486	2.4243806804105192E-4
26-27	1.9625938841418488E-4	3.9251877682836976E-4	0.0	0.00584160297279868	2.4243806804105192E-4
28-29	1.9625938841418488E-4	3.9251877682836976E-4	0.0	0.019141062705336387	2.482104029944103E-4
30-31	2.0780405832090165E-4	4.2715278654852003E-4	0.0	0.021051705574898008	2.712997428078438E-4
32-33	2.0780405832090165E-4	4.386974564552368E-4	0.0	0.025380956789916795	2.943890826212773E-4
34-35	2.3089339813433515E-4	5.368271506623293E-4	0.0	0.03080117931112031	3.117060874813525E-4
36-37	2.539827379477687E-4	6.23412174962705E-4	0.0	0.03929805636246385	3.232507573880692E-4
38-39	2.539827379477687E-4	6.291845099160633E-4	0.0	0.062433574855524226	3.290230923414276E-4
40-41	2.770720777612022E-4	7.15769534216439E-4	0.0	0.08844948849031045	3.4056776224814433E-4
42-43	2.770720777612022E-4	7.734928837500228E-4	0.0	0.10475056239859451	3.521124321548611E-4
44-45	2.770720777612022E-4	8.427609031903234E-4	0.0	0.12341252130280214	3.578847671082195E-4
46-47	2.770720777612022E-4	8.773949129104737E-4	0.0	0.14334439389674863	3.694294370149363E-4
48-49	2.770720777612022E-4	8.83167247863832E-4	0.0	0.16271635000021933	3.80974106921653E-4
50-51	2.943890826212773E-4	0.001004386281884358	0.0	0.18124554520049974	3.9251877682836976E-4
52-53	3.001614175746357E-4	0.0010159309517910747	0.0	0.22809958801690972	4.040634467350865E-4
54-55	3.001614175746357E-4	0.0010159309517910747	0.0	0.2837448969672845	4.156081166418033E-4
56-57	3.001614175746357E-4	0.0010274756216977915	0.0	0.33897459780101746	4.386974564552368E-4
58-59	3.001614175746357E-4	0.00103324795665115	0.0	0.37461299380305213	4.617867962686703E-4
60-61	3.290230923414276E-4	0.0010447926265578665	0.0	0.399093466340245	4.906484710354623E-4
62-63	3.578847671082195E-4	0.0010909713061847339	0.0	0.41813639935137425	4.964208059888206E-4
64-65	3.578847671082195E-4	0.0012237350101119765	0.0	0.42627539163560957	4.964208059888206E-4
66-67	3.694294370149363E-4	0.0012237350101119765	0.0	0.433207965914593	4.964208059888206E-4
68-69	3.80974106921653E-4	0.0012352796800186932	0.0	0.439649891722541	5.079654758955374E-4
70-71	3.867464418750114E-4	0.0012352796800186932	0.0	0.4448161315057967	5.368271506623293E-4
72-73	4.2138045159516166E-4	0.0012410520149720517	0.0	0.4485970109002464	5.772334953358379E-4
74-75	4.2715278654852003E-4	0.0012814583596455602	0.0	0.4515062677167391	5.830058302891963E-4
76-77	4.3292512150187846E-4	0.001293003029552277	0.0	0.454259671489491	5.94550500195913E-4
78-79	4.5024212636195356E-4	0.001293003029552277	0.0	0.4560664123298922	6.003228351492714E-4
80-81	4.5024212636195356E-4	0.0012987753645056352	0.0	0.45674177551943507	6.176398400093465E-4
82-83	4.5024212636195356E-4	0.0013103200344123521	0.0	0.45722665165551724	6.349568448694217E-4
84-85	4.5024212636195356E-4	0.001327637039272427	0.0	0.45733632601963103	6.407291798227801E-4
86-87	4.5024212636195356E-4	0.001327637039272427	0.0	0.45744600038374483	6.580461846828552E-4
88	4.617867962686703E-4	0.0013622710489925775	0.0	0.45745754505365155	6.695908545895719E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10725	0.0	49.778812	1
GTATCAA	19935	0.0	38.76354	1
TCAACGC	25070	0.0	30.116693	4
ATCAACG	25200	0.0	29.961332	3
TATCAAC	25915	0.0	29.36269	2
CAACGCA	25695	0.0	29.351292	5
AACGCAG	26430	0.0	28.598927	6
ACGCAGA	30935	0.0	24.393196	7
CGCAGAG	31425	0.0	23.985981	8
GTACATG	29155	0.0	22.466219	1
TACATGG	29930	0.0	21.546064	2
GCAGAGT	36335	0.0	20.582108	9
ACATGGG	30895	0.0	20.394962	3
GTGGTAT	5010	0.0	19.796183	1
GAGTACT	18550	0.0	18.975565	12-13
AGAGTAC	32325	0.0	18.814081	10-11
CATGGGG	23865	0.0	18.232595	4
CAGAGTA	34995	0.0	18.144447	10-11
TGGTATC	5285	0.0	17.328701	2
GTACTTT	20275	0.0	17.226908	14-15
>>END_MODULE