FastQCFastQC Report
Fri 27 May 2016
SRR2139364_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139364_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8741230
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110900.049.9681781
GTATCAA196600.036.8543131
TCAACGC241250.029.3288064
ATCAACG242500.029.2126753
CAACGCA249050.028.4614455
TATCAAC254000.028.0071662
AACGCAG255400.027.6872546
ACGCAGA294550.024.0649247
CGCAGAG298900.023.7431328
GTACATG258050.020.7498111
GCAGAGT348650.020.2332619
TACATGG262650.019.933372
GTGGTAT53800.019.7471831
GAGTACT201850.019.52709212-13
ACATGGG264100.019.083753
TGGTATC52150.018.9051932
AGAGTAC315450.018.71890610-11
CAGAGTA339700.018.289610-11
GTACTTT228750.017.20317314-15
CATGGGG198350.017.1183554
Produced by FastQC (version 0.11.5)