##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139364_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8741230
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13128243965666	33.0	31.0	34.0	30.0	34.0
2	32.359834485535785	34.0	31.0	34.0	30.0	34.0
3	32.40215347268062	34.0	31.0	34.0	30.0	34.0
4	36.04877139716035	37.0	35.0	37.0	35.0	37.0
5	35.90388904078716	37.0	35.0	37.0	35.0	37.0
6	35.9265866474169	37.0	35.0	37.0	35.0	37.0
7	35.87928643909381	37.0	35.0	37.0	35.0	37.0
8	35.879213794854955	37.0	35.0	37.0	35.0	37.0
9	37.648528410761415	39.0	37.0	39.0	35.0	39.0
10-11	37.52814335053534	39.0	37.0	39.0	35.0	39.0
12-13	37.49403974040267	39.0	37.0	39.0	35.0	39.0
14-15	38.74812309022872	40.0	38.0	41.0	34.5	41.0
16-17	38.7336113453141	40.0	38.0	41.0	34.0	41.0
18-19	38.719964181242226	40.0	38.0	41.0	34.0	41.0
20-21	38.71286363497775	40.0	38.0	41.0	34.0	41.0
22-23	38.636257062128756	40.0	38.0	41.0	34.0	41.0
24-25	38.5713218221826	40.0	38.0	41.0	34.0	41.0
26-27	38.39581317280743	40.0	38.0	41.0	34.0	41.0
28-29	38.25872641956464	40.0	38.0	41.0	34.0	41.0
30-31	38.121482122491074	40.0	37.5	41.0	33.0	41.0
32-33	38.031033289297255	40.0	37.0	41.0	33.0	41.0
34-35	38.089335811518154	40.0	37.5	41.0	33.0	41.0
36-37	38.04650750330039	40.0	37.0	41.0	33.0	41.0
38-39	37.98563015647268	40.0	37.0	41.0	33.0	41.0
40-41	37.912647763394574	40.0	37.0	41.0	33.0	41.0
42-43	37.88274225620708	40.0	37.0	41.0	33.0	41.0
44-45	37.91567944513314	40.0	37.0	41.0	33.0	41.0
46-47	37.89987422525647	40.0	37.0	41.0	33.0	41.0
48-49	37.81996749737898	40.0	37.0	41.0	33.0	41.0
50-51	37.73724505900718	40.0	37.0	41.0	32.5	41.0
52-53	37.50672293523904	40.0	36.0	41.0	32.0	41.0
54-55	37.385677895981345	39.0	36.0	41.0	31.0	41.0
56-57	37.32293663566325	39.0	36.0	41.0	31.5	41.0
58-59	37.16358155663076	39.0	35.5	41.0	31.0	41.0
60-61	36.97009018974141	39.0	35.0	40.5	31.0	41.0
62-63	36.7213790078793	38.5	35.0	40.0	31.0	41.0
64-65	36.427655506645934	38.0	35.0	40.0	30.5	41.0
66-67	36.143819475018134	37.5	34.5	40.0	30.0	41.0
68-69	35.8649822151351	37.0	34.0	40.0	30.0	41.0
70-71	35.56301757069497	36.5	34.0	39.0	29.5	41.0
72-73	35.24986238932209	36.0	34.0	39.0	29.0	40.5
74-75	34.89657250837611	35.5	34.0	38.5	29.0	40.0
76-77	33.567511429903014	34.5	31.5	36.5	27.5	39.0
78-79	34.2940879016858	35.0	33.0	37.0	29.0	39.0
80-81	34.23873558972932	35.0	34.0	37.0	29.0	39.0
82-83	33.95300804849912	35.0	33.0	36.0	29.0	38.5
84-85	33.767263198547056	35.0	33.0	36.0	29.0	37.0
86-87	33.52787336340053	35.0	33.0	35.5	29.0	37.0
88-89	33.41495093936608	35.0	33.0	35.0	29.0	36.5
90-91	33.232174925539326	35.0	33.0	35.0	29.0	36.0
92-93	33.124934117791234	35.0	33.0	35.0	29.0	36.0
94-95	33.11854657519068	35.0	33.0	35.0	29.0	36.0
96-97	33.15674220881411	35.0	33.0	35.0	29.0	35.0
98-99	33.260861910382204	34.5	33.0	35.0	29.0	35.0
100	31.91673893416993	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	4.0
20	46.0
21	256.0
22	1260.0
23	4690.0
24	12504.0
25	27078.0
26	49933.0
27	79069.0
28	112997.0
29	151792.0
30	191174.0
31	236635.0
32	292398.0
33	368040.0
34	487268.0
35	668611.0
36	1025620.0
37	1753076.0
38	2188232.0
39	1052125.0
40	38420.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.78710890801409	20.805092647144622	14.97967677317723	23.428121671664055
2	14.405215284347856	21.605071597475412	41.57127772636117	22.41843539181557
3	17.790791456122307	26.72099921864543	31.059759324488663	24.428450000743602
4	12.439965542606704	18.752864299417816	39.42601899275045	29.381151165225035
5	12.944025039954333	38.23090114320296	35.82145762095266	13.003616195890052
6	29.123818959116736	38.62650908396187	19.219915275081426	13.029756681839972
7	25.97052131107407	33.14820683130406	23.55389344520165	17.32737841242022
8	24.203321500521096	36.84624475045274	21.436983124800516	17.51345062422565
9	25.51732422096204	17.068227240331165	21.4436755468052	35.9707729919016
10-11	23.823243410824336	26.982009396846895	29.311715856921737	19.883031335407033
12-13	24.790241190313033	24.648556324453192	29.30701972148084	21.254182763752926
14-15	21.92010163329417	26.03752561138421	26.981603275511567	25.06076947981005
16-17	21.358837833669902	29.22076154050669	27.630679990353645	21.789720635469763
18-19	21.368213734875972	28.37062712741994	29.431977588488202	20.82918154921589
20-21	22.235767086277797	27.49593420502361	29.274004121195386	20.994294587503212
22-23	21.97137950052291	27.417775992786808	29.13359284355927	21.47725166313101
24-25	21.73616071001939	27.562001345586868	29.15875985119917	21.543078093194566
26-27	21.6859248731276	27.741184087414766	29.061745998247794	21.51114504120984
28-29	21.57997848304185	27.84410893936702	29.092847473040507	21.48306510455063
30-31	21.89213093369457	27.638586306004115	28.991151152873044	21.47813160742827
32-33	21.675521918904387	27.77899676539815	28.943583311744035	21.60189800395343
34-35	21.856378665923813	27.800631349701966	28.919554453162327	21.423435531211894
36-37	21.80832153545651	27.806890106676573	28.84735838192874	21.537429975938178
38-39	21.69580548586981	27.87397928197598	28.887306491602523	21.542908740551688
40-41	21.898183557329226	27.747079408771025	28.76938224386189	21.585354790037854
42-43	21.791317165619624	27.87459222624929	28.5429209061226	21.791169702008485
44-45	21.903318006237473	27.935343881723888	28.38573433809794	21.775603773940695
46-47	21.947084721013226	27.93732306724881	28.157778881257588	21.957813330480374
48-49	21.893198376236096	28.093412397908292	28.12019084961952	21.893198376236096
50-51	21.90510134783207	28.175963728568526	28.012093326688227	21.906841596911175
52-53	22.04468927638047	28.042818885133908	27.92353793353807	21.988953904947543
54-55	22.026331834876846	27.967069258788918	27.990250588126365	22.016348318207875
56-57	21.999325045251798	28.060451133862824	27.958945827517862	21.98127799336752
58-59	21.972431886807612	28.09625339454659	27.94491486366099	21.986399854984807
60-61	22.04523365591301	28.055035025830783	28.003006728020296	21.896724590235912
62-63	21.986345731563294	28.146805009175264	27.94911649528672	21.91773276397472
64-65	21.96574590839211	28.105915695039492	27.864809365812874	22.063529030755525
66-67	22.033747348412394	28.121516444433105	27.74521268886967	22.09952351828484
68-69	21.971295637683397	28.110657572046026	27.828976485755653	22.089070304514923
70-71	21.96176767322052	27.963000014737116	27.88820583316284	22.187026478879524
72-73	22.026178830732704	27.962870362356735	27.798410493896476	22.212540313014085
74-75	21.962853252901322	28.08528855327254	27.767960895082144	22.183897298743993
76-77	22.092582052741417	27.99457575336037	27.709664654459377	22.20317753943884
78-79	22.035157703013855	27.997136724316153	27.721250341273358	22.246455231396638
80-81	22.03419994242481	28.025254826114544	27.725179083995492	22.215366147465154
82-83	22.080155449016864	28.007440824012104	27.80969454355829	22.10270918341275
84-85	22.124350101585836	27.961900270719216	27.80069557951113	22.113054048183816
86-87	22.0472784040144	27.977207940089738	27.830941300522355	22.144572355373505
88-89	22.065008362292804	27.956035842568955	27.797366132703132	22.18158966243511
90-91	22.13336038892714	27.898876142515938	27.87224515466868	22.095518313888242
92-93	22.11900073057706	27.819130513303865	27.940716595795745	22.121152160323334
94-95	21.983182102126776	27.942118289481314	27.956053255981395	22.118646352410515
96-97	22.050002415717444	28.015499623917616	28.063536304107807	21.870961656257133
98-99	23.05860756164658	30.021112414475414	28.252838991836114	18.66744103204189
100	22.1036000715133	31.07429074607511	22.044753675998756	24.777355506412828
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1522.0
1	1411.5
2	2067.0
3	3017.5
4	3987.0
5	6233.5
6	8311.0
7	10139.5
8	11692.5
9	12055.0
10	11367.0
11	10421.5
12	11042.5
13	14177.5
14	21672.5
15	32610.0
16	42146.5
17	48261.5
18	50711.5
19	49668.0
20	46464.0
21	42917.0
22	41367.5
23	43707.5
24	50277.0
25	61206.0
26	76372.5
27	93739.5
28	113419.0
29	135092.5
30	156761.0
31	178828.5
32	201370.5
33	222488.0
34	242768.5
35	261976.0
36	280969.5
37	298713.0
38	312427.0
39	322586.5
40	333199.0
41	344852.0
42	353309.5
43	359381.5
44	362723.5
45	365961.0
46	366670.0
47	362265.0
48	357195.0
49	349247.5
50	339542.5
51	327573.5
52	310592.0
53	290060.5
54	270796.5
55	251861.0
56	230660.5
57	207605.0
58	183193.5
59	158559.0
60	133027.0
61	108831.0
62	86414.0
63	66890.5
64	50835.0
65	38235.0
66	28765.5
67	21912.0
68	16768.0
69	12092.0
70	8500.0
71	6063.5
72	4150.5
73	2757.0
74	1788.5
75	1149.5
76	764.5
77	493.0
78	311.0
79	211.0
80	154.5
81	109.0
82	77.0
83	55.5
84	38.5
85	30.0
86	24.0
87	17.0
88	10.0
89	5.5
90	4.0
91	3.0
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0012126439871734298
18-19	9.152030091874942E-5
20-21	0.0
22-23	0.006841997231622658
24-25	0.008205380795758287
26-27	0.020378334075768705
28-29	0.016379624251419672
30-31	0.0018348155680660673
32-33	0.006137344106862425
34-35	0.032619928955408446
36-37	0.005474478427169617
38-39	0.0028805887713951365
40-41	0.0074609947801732674
42-43	0.007384432307316008
44-45	0.008767315969487599
46-47	0.005108886520884299
48-49	9.994119823318497E-4
50-51	0.0027049455747243044
52-53	0.00116229314410627
54-55	0.005963742673978723
56-57	0.001023404640091677
58-59	0.001403069008973979
60-61	8.057976314752422E-4
62-63	0.0015929470191579068
64-65	3.8062824175106024E-4
66-67	0.005839629706280948
68-69	0.004180474227474984
70-71	0.0018255102915300107
72-73	0.0016290191186510488
74-75	6.093433871381288E-4
76-77	8.070102104573203E-4
78-79	7.67016997649473E-4
80-81	0.0010341067986373268
82-83	0.007272361309567812
84-85	7.149349773787425E-4
86-87	7.238050955010157E-5
88-89	5.575564414018818E-4
90-91	2.3837470760921118E-4
92-93	0.0025301385712958964
94-95	0.0036380989133438825
96-97	1.1106734528814638E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	197.0
20-21	629.0
22-23	1356.0
24-25	3359.0
26-27	3731.0
28-29	7622.0
30-31	18024.0
32-33	23565.0
34-35	32056.0
36-37	45754.0
38-39	53021.0
40-41	57730.0
42-43	69087.0
44-45	77156.0
46-47	75692.0
48-49	67790.0
50-51	60139.0
52-53	55796.0
54-55	59813.0
56-57	65315.0
58-59	66268.0
60-61	65371.0
62-63	64573.0
64-65	68900.0
66-67	73055.0
68-69	74334.0
70-71	74247.0
72-73	73221.0
74-75	74663.0
76-77	73146.0
78-79	79415.0
80-81	81281.0
82-83	80908.0
84-85	84030.0
86-87	90185.0
88-89	100300.0
90-91	109608.0
92-93	122365.0
94-95	148054.0
96-97	343162.0
98-99	624308.0
100-101	5392004.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.83563956491852
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.62265255541617	76.03195049043211
2	7.218118768231719	12.247074443169597
3	1.7166909104963828	4.369097139817288
4	0.6629099696037909	2.249535649811932
5	0.30154956972034636	1.2791075303875787
6	0.15737665457130648	0.8010689485886426
7	0.09000185995385793	0.5344755745852436
8	0.06168176202227771	0.41862493845208254
9	0.04454912824692933	0.34014184081990445
>10	0.12233348216177883	1.5802772855987746
>50	0.0018838027978457094	0.10388885929943703
>100	2.5153677764412765E-4	0.04475729903733167
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1440037614843678E-5
2	0.0	2.2880075229687356E-5	0.0	0.0	1.1440037614843678E-5
3	0.0	2.2880075229687356E-5	0.0	0.0	1.1440037614843678E-5
4	0.0	4.576015045937471E-5	0.0	0.0	1.1440037614843678E-5
5	0.0	4.576015045937471E-5	0.0	1.1440037614843678E-5	1.1440037614843678E-5
6	3.432011284453103E-5	4.576015045937471E-5	0.0	1.1440037614843678E-5	1.1440037614843678E-5
7	3.432011284453103E-5	4.576015045937471E-5	0.0	1.1440037614843678E-5	1.1440037614843678E-5
8	3.432011284453103E-5	6.864022568906206E-5	0.0	2.2880075229687356E-5	1.1440037614843678E-5
9	3.432011284453103E-5	1.029603385335931E-4	0.0	2.2880075229687356E-5	1.1440037614843678E-5
10-11	3.432011284453103E-5	1.029603385335931E-4	0.0	2.2880075229687356E-5	1.1440037614843678E-5
12-13	4.576015045937471E-5	1.029603385335931E-4	0.0	6.292020688164022E-5	1.1440037614843678E-5
14-15	5.148016926679655E-5	1.601605266078115E-4	0.0	8.008026330390574E-5	2.2880075229687356E-5
16-17	1.4300047018554597E-4	1.601605266078115E-4	0.0	1.1440037614843677E-4	2.2880075229687356E-5
18-19	1.487204889929678E-4	1.6588054541523333E-4	0.0	1.2584041376328045E-4	2.8600094037109192E-5
20-21	1.487204889929678E-4	1.7160056422265517E-4	0.0	2.0020065825976437E-4	3.432011284453103E-5
22-23	1.5444050780038965E-4	1.9448063945234253E-4	0.0	4.747615610160126E-4	3.432011284453103E-5
24-25	1.601605266078115E-4	2.1736071468202988E-4	0.0	0.001418564664240616	4.576015045937471E-5
26-27	1.601605266078115E-4	2.2880075229687356E-4	0.0	0.0038896127890468506	4.576015045937471E-5
28-29	1.601605266078115E-4	2.4024078991171722E-4	0.0	0.012332360548801484	5.148016926679655E-5
30-31	1.601605266078115E-4	2.688408839488264E-4	0.0	0.013796685363501475	5.720018807421839E-5
32-33	1.601605266078115E-4	2.7456090275624826E-4	0.0	0.01733737700529559	5.720018807421839E-5
34-35	1.6588054541523333E-4	3.088810156007793E-4	0.0	0.02165027118609166	6.864022568906206E-5
36-37	1.7160056422265517E-4	3.4320112844531033E-4	0.0	0.02913777580500685	6.864022568906206E-5
38-39	1.7160056422265517E-4	3.4892114725273217E-4	0.0	0.049003401123182896	7.43602444964839E-5
40-41	2.059206770671862E-4	4.232813917492161E-4	0.0	0.07049351178266675	9.152030091874942E-5
42-43	2.059206770671862E-4	4.3472142936405977E-4	0.0	0.08479355880122133	9.152030091874942E-5
44-45	2.059206770671862E-4	4.862015986308563E-4	0.0	0.10108989238356617	9.152030091874942E-5
46-47	2.059206770671862E-4	5.376817678976528E-4	0.0	0.11876475049849963	9.152030091874942E-5
48-49	2.059206770671862E-4	5.491218055124965E-4	0.0	0.13524984470148937	9.152030091874942E-5
50-51	2.1736071468202988E-4	6.40642106431246E-4	0.0	0.15132309755034473	9.152030091874942E-5
52-53	2.1736071468202988E-4	6.520821440460896E-4	0.0	0.1950011611638179	9.152030091874942E-5
54-55	2.1736071468202988E-4	6.635221816609333E-4	0.0	0.24580636821133867	9.724031972617126E-5
56-57	2.1736071468202988E-4	6.692422004683551E-4	0.0	0.29534173108361184	1.029603385335931E-4
58-59	2.1736071468202988E-4	6.864022568906207E-4	0.0	0.32701919523911394	1.029603385335931E-4
60-61	2.2308073348945172E-4	6.978422945054643E-4	0.0	0.3493673087197111	1.0868035734101494E-4
62-63	2.2880075229687356E-4	7.09282332120308E-4	0.0	0.3666932456873918	1.2584041376328045E-4
64-65	2.2880075229687356E-4	7.893625954242138E-4	0.0	0.3745239514347523	1.2584041376328045E-4
66-67	2.2880075229687356E-4	7.893625954242138E-4	0.0	0.38112485313851713	1.2584041376328045E-4
68-69	2.4024078991171722E-4	8.17962689461323E-4	0.0	0.38781155512439325	1.3728045137812413E-4
70-71	2.4024078991171722E-4	8.408427646910103E-4	0.0	0.3928623317313467	1.601605266078115E-4
72-73	2.8600094037109193E-4	8.465627834984322E-4	0.0	0.3972495861566393	1.7160056422265517E-4
74-75	2.8600094037109193E-4	8.694428587281195E-4	0.0	0.4006930374787072	1.7160056422265517E-4
76-77	2.8600094037109193E-4	9.037629715726506E-4	0.0	0.40378184763471503	1.7160056422265517E-4
78-79	2.8600094037109193E-4	9.152030091874943E-4	0.0	0.40572665402923846	1.7160056422265517E-4
80-81	2.8600094037109193E-4	9.209230279949161E-4	0.0	0.40638445619209196	1.77320583030077E-4
82-83	2.8600094037109193E-4	9.266430468023379E-4	0.0	0.40680201756503376	1.8304060183749885E-4
84-85	2.8600094037109193E-4	9.266430468023379E-4	0.0	0.4070250982985232	1.8304060183749885E-4
86-87	2.8600094037109193E-4	9.609631596468689E-4	0.0	0.4070880185054049	1.8304060183749885E-4
88	2.8600094037109193E-4	9.838432348765562E-4	0.0	0.40709373852421227	1.8304060183749885E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11090	0.0	49.968178	1
GTATCAA	19660	0.0	36.854313	1
TCAACGC	24125	0.0	29.328806	4
ATCAACG	24250	0.0	29.212675	3
CAACGCA	24905	0.0	28.461445	5
TATCAAC	25400	0.0	28.007166	2
AACGCAG	25540	0.0	27.687254	6
ACGCAGA	29455	0.0	24.064924	7
CGCAGAG	29890	0.0	23.743132	8
GTACATG	25805	0.0	20.749811	1
GCAGAGT	34865	0.0	20.233261	9
TACATGG	26265	0.0	19.93337	2
GTGGTAT	5380	0.0	19.747183	1
GAGTACT	20185	0.0	19.527092	12-13
ACATGGG	26410	0.0	19.08375	3
TGGTATC	5215	0.0	18.905193	2
AGAGTAC	31545	0.0	18.718906	10-11
CAGAGTA	33970	0.0	18.2896	10-11
GTACTTT	22875	0.0	17.203173	14-15
CATGGGG	19835	0.0	17.118355	4
>>END_MODULE