FastQCFastQC Report
Fri 27 May 2016
SRR2139363_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139363_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7740441
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86650.050.2128371
GTATCAA154650.037.75141
TCAACGC181800.031.161194
ATCAACG181950.031.15993
CAACGCA186100.030.4650425
AACGCAG193450.029.3993456
TATCAAC198050.029.0303442
ACGCAGA221500.025.6763117
CGCAGAG225600.025.1506398
GTACATG214900.021.7338451
TACATGG212650.021.4000952
GCAGAGT271950.020.6191869
GTGGTAT45450.020.5136361
ACATGGG216700.020.4674533
GAGTACT156800.019.62205312-13
TGGTATC42650.019.5702342
AGAGTAC242300.019.10204510-11
CAGAGTA264800.018.4765510-11
CATGGGG159900.017.4368654
GTACTTT177250.017.37126714-15
Produced by FastQC (version 0.11.5)