FastQCFastQC Report
Fri 27 May 2016
SRR2139362_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139362_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7591741
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90300.051.0643461
GTATCAA162700.037.751981
TCAACGC192750.031.0858024
ATCAACG194600.030.8130193
CAACGCA199000.030.109495
TATCAAC205750.029.5733592
AACGCAG206950.028.9955986
ACGCAGA239000.025.1072777
CGCAGAG242400.024.7551128
GTACATG233650.022.1972731
GTGGTAT45300.022.1750951
TACATGG231250.021.9683762
TGGTATC43300.021.7685492
ACATGGG237400.020.8400573
GCAGAGT286350.020.7392529
GAGTACT158650.020.12531512-13
CATGGGG183150.018.9912454
AGAGTAC257950.018.71697210-11
CAGAGTA278400.018.65349610-11
GTACTTT177650.017.87376214-15
Produced by FastQC (version 0.11.5)