##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139362_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7591741
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17555090459487	33.0	31.0	34.0	30.0	34.0
2	32.398880046092195	34.0	31.0	34.0	31.0	34.0
3	32.44449092770683	34.0	31.0	34.0	31.0	34.0
4	36.08041804903513	37.0	35.0	37.0	35.0	37.0
5	35.93693422892061	37.0	35.0	37.0	35.0	37.0
6	35.959857034111145	37.0	35.0	37.0	35.0	37.0
7	35.91451644622755	37.0	35.0	37.0	35.0	37.0
8	35.91230127582066	37.0	35.0	37.0	35.0	37.0
9	37.686723506505295	39.0	37.0	39.0	35.0	39.0
10-11	37.56993527571607	39.0	37.0	39.0	35.0	39.0
12-13	37.5343083358613	39.0	37.0	39.0	35.0	39.0
14-15	38.8085532027502	40.0	38.0	41.0	35.0	41.0
16-17	38.794268864019465	40.0	38.0	41.0	35.0	41.0
18-19	38.77067532994079	40.0	38.0	41.0	34.0	41.0
20-21	38.75732535048936	40.0	38.0	41.0	34.0	41.0
22-23	38.68103623499907	40.0	38.0	41.0	34.0	41.0
24-25	38.61621616491272	40.0	38.0	41.0	34.0	41.0
26-27	38.44631563894258	40.0	38.0	41.0	34.0	41.0
28-29	38.31124103388728	40.0	38.0	41.0	34.0	41.0
30-31	38.178308793807766	40.0	38.0	41.0	33.5	41.0
32-33	38.089712254268214	40.0	37.5	41.0	33.0	41.0
34-35	38.14806776373001	40.0	38.0	41.0	33.0	41.0
36-37	38.11051045179529	40.0	38.0	41.0	33.0	41.0
38-39	38.04528863762849	40.0	37.0	41.0	33.0	41.0
40-41	37.97079600906294	40.0	37.0	41.0	33.0	41.0
42-43	37.93600199904842	40.0	37.0	41.0	33.0	41.0
44-45	37.96507518991951	40.0	37.0	41.0	33.0	41.0
46-47	37.94700417684534	40.0	37.0	41.0	33.0	41.0
48-49	37.862699552404344	40.0	37.0	41.0	33.0	41.0
50-51	37.77541800810484	40.0	37.0	41.0	33.0	41.0
52-53	37.54473795771278	40.0	36.5	41.0	32.0	41.0
54-55	37.41796930621661	39.0	36.0	41.0	32.0	41.0
56-57	37.349023982796425	39.0	36.0	41.0	32.0	41.0
58-59	37.18529094687872	39.0	35.5	41.0	31.0	41.0
60-61	36.98707524211362	39.0	35.0	40.5	31.0	41.0
62-63	36.73235561006081	38.5	35.0	40.0	31.0	41.0
64-65	36.43436778871006	38.0	35.0	40.0	30.5	41.0
66-67	36.145832271839595	37.5	35.0	40.0	30.0	41.0
68-69	35.855761964206664	37.0	34.0	39.5	30.0	41.0
70-71	35.554510710515245	36.5	34.0	39.0	30.0	41.0
72-73	35.24056866671167	36.0	34.0	39.0	29.0	40.5
74-75	34.884147319743995	35.5	34.0	38.5	29.0	40.0
76-77	33.558819621514104	34.5	31.5	36.5	28.0	39.0
78-79	34.283124484351475	35.0	33.0	37.0	29.0	39.0
80-81	34.231075846872685	35.0	34.0	37.0	29.0	39.0
82-83	33.95442589660082	35.0	33.0	36.0	29.0	38.5
84-85	33.77122958651714	35.0	33.0	36.0	29.0	37.0
86-87	33.536759832575406	35.0	33.0	35.5	29.0	37.0
88-89	33.42671622460651	35.0	33.0	35.0	29.0	36.5
90-91	33.247820548731774	35.0	33.0	35.0	29.0	36.0
92-93	33.14212566523875	35.0	33.0	35.0	29.0	36.0
94-95	33.13797981973424	35.0	33.0	35.0	29.0	36.0
96-97	33.17542548663093	35.0	33.0	35.0	29.0	35.0
98-99	33.27835344392584	35.0	33.0	35.0	29.0	35.0
100	31.980639130534378	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	4.0
19	7.0
20	31.0
21	209.0
22	1165.0
23	4008.0
24	10725.0
25	23455.0
26	42842.0
27	69060.0
28	98543.0
29	131981.0
30	167241.0
31	206914.0
32	255778.0
33	323337.0
34	428917.0
35	593330.0
36	925297.0
37	1601750.0
38	1946593.0
39	739589.0
40	20963.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.34035148727433	20.30271057754876	14.682813162726857	23.67412477245006
2	14.593979772753018	21.313743621356895	41.29544742048595	22.796829185404135
3	18.08691840251136	26.226197653476323	30.728629967750482	24.958253976261837
4	12.60953351932495	18.303616825655254	39.01867029165909	30.068179363360702
5	13.111933086231472	38.06890935820913	35.68766373879193	13.13149381676746
6	29.78231212050042	38.16540896218667	18.882045633537814	13.170233283775094
7	26.450928713189768	32.866558540392774	23.171997042575608	17.510515703841847
8	24.543727788359455	36.58077067971243	21.20118707964182	17.6743144522863
9	25.63544062362515	16.944231493702368	21.24747422856947	36.17285365410301
10-11	24.089615819298345	26.83864963763248	29.036149815457325	20.03558472761185
12-13	24.955375848570178	24.335188726801928	29.124887163563667	21.584548261064228
14-15	22.058227486949306	25.77576737667947	26.783546751660786	25.38245838471044
16-17	21.644720419987472	28.945374888611248	27.403886240878965	22.006018450522316
18-19	21.692775974720938	28.130130166521838	29.094408828967598	21.082685029789623
20-21	22.50760321242311	27.30252065196888	28.9565684273311	21.23330770827691
22-23	22.196253063403393	27.21361443526005	28.831793628120945	21.75833887321561
24-25	22.031390256256994	27.328705189814233	28.85958205710846	21.780322496820308
26-27	21.937532650091544	27.535388991336706	28.750596971389868	21.776481387181885
28-29	21.870408850667665	27.649955344684702	28.776341492621714	21.703294312025918
30-31	22.19627692798362	27.398367069510027	28.71482977436281	21.690526228143543
32-33	22.011423629668776	27.53073744299431	28.64899649710499	21.80884243023192
34-35	22.117068964889768	27.56310733896905	28.63391308346807	21.685910612673116
36-37	22.105662724940437	27.539746289996085	28.573398050543293	21.78119293452019
38-39	21.936207088095415	27.668308113627678	28.650199572947383	21.745285225329525
40-41	22.24436936276483	27.496318485892683	28.502923351462766	21.756388799879716
42-43	22.094476472850168	27.615361622003814	28.32578557214799	21.964376332998032
44-45	22.195055921390146	27.693110705397224	28.16173779392839	21.95009557928424
46-47	22.266896868306034	27.687941545998303	27.92840268036172	22.116758905333942
48-49	22.16317513145497	27.803852575041137	27.953204852653197	22.079767440850688
50-51	22.149804713884535	27.898382415264123	27.885323148058642	22.0664897227927
52-53	22.30920097004612	27.790024453542234	27.78880738315919	22.11196719325245
54-55	22.309621169405645	27.671176789989794	27.813146987983554	22.206055052621004
56-57	22.267809092138023	27.84403020542961	27.780373030082924	22.107787672349446
58-59	22.25773144372548	27.87519172142626	27.801589237942707	22.06548759690555
60-61	22.297263671217948	27.83170549295344	27.825012024610835	22.04601881121778
62-63	22.23823238158227	27.896355832944984	27.729571184841323	22.135840600631415
64-65	22.248222379928865	27.85680533196566	27.70612026109765	22.188852027007826
66-67	22.30904713996957	27.86813212549233	27.64076253181601	22.182058202722086
68-69	22.253771417389395	27.8524720878431	27.684386830800374	22.20936966396713
70-71	22.233227885986654	27.70054181418123	27.735293536044097	22.33093676378802
72-73	22.300813458448072	27.718156055484805	27.690511787500068	22.29051869856706
74-75	22.14607054857261	27.835563001118473	27.659722316625455	22.358644133683462
76-77	22.25214573448588	27.732812828758412	27.64556375901958	22.369477677736125
78-79	22.255533465910307	27.794512826543627	27.583014819157363	22.366938888388702
80-81	22.226938959605466	27.773733452564574	27.65479777905955	22.344529808770414
82-83	22.27367028294256	27.739098172244265	27.716668612797324	22.270562932015856
84-85	22.323800166897616	27.717037363615916	27.687770529380213	22.27139194010626
86-87	22.18435449474319	27.718023105814332	27.73661423512907	22.361008164313407
88-89	22.26724079278741	27.686709816892407	27.708746944966013	22.337302445354172
90-91	22.276183528062173	27.67070013208398	27.76248467021107	22.290631669642778
92-93	22.261860736063593	27.633559976938038	27.825314931454635	22.279264355543734
94-95	22.11763819737726	27.729982399423704	27.79818913988752	22.354190263311512
96-97	22.155822802749796	27.881964624333687	27.89984837404856	22.062364198867954
98-99	22.287559033730023	28.707453714613308	26.99573548091442	22.009251770742257
100	20.52102036752825	28.569920760641455	28.037218006029736	22.87184086580056
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1112.0
1	996.5
2	1470.5
3	2214.0
4	2925.0
5	4649.5
6	6248.0
7	7642.0
8	8957.0
9	9209.0
10	8495.0
11	7696.5
12	7985.0
13	10087.5
14	15760.5
15	24252.5
16	31497.5
17	35928.0
18	37343.0
19	35751.5
20	32569.5
21	29453.0
22	27898.0
23	29094.5
24	33456.0
25	41645.5
26	53610.0
27	67407.0
28	82422.5
29	99103.5
30	115662.5
31	133044.5
32	152934.5
33	172065.5
34	190028.5
35	205752.0
36	222393.5
37	239418.0
38	251411.0
39	259730.5
40	269934.0
41	281409.5
42	287908.5
43	292882.0
44	297003.5
45	301615.0
46	304855.0
47	303422.0
48	300562.0
49	294802.0
50	286594.5
51	276547.5
52	264061.0
53	249866.0
54	233862.0
55	216215.5
56	198572.5
57	179874.0
58	159856.0
59	138880.5
60	116873.0
61	95853.5
62	75770.5
63	58268.0
64	43929.5
65	32547.0
66	24557.0
67	19035.0
68	14584.5
69	10569.0
70	7488.5
71	5337.0
72	3758.0
73	2578.5
74	1745.0
75	1140.0
76	713.0
77	437.5
78	278.0
79	189.5
80	142.5
81	111.5
82	84.5
83	57.5
84	37.5
85	23.5
86	16.0
87	12.0
88	8.0
89	7.0
90	5.5
91	3.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.3172209115142364E-5
2	1.3172209115142364E-5
3	0.0
4	1.3172209115142364E-5
5	0.0
6	0.0
7	0.0
8	1.3172209115142364E-5
9	1.3172209115142364E-5
10-11	1.3172209115142364E-5
12-13	0.0
14-15	0.0
16-17	0.0011328099839022433
18-19	5.268883646056946E-5
20-21	0.0
22-23	0.007232283967133025
24-25	0.008472308398350285
26-27	0.021438582251331887
28-29	0.016653948746187097
30-31	0.0020254689191464476
32-33	0.006631604151357778
34-35	0.033953627005417104
36-37	0.005719684050750498
38-39	0.0025779833313444764
40-41	0.007690334103033151
42-43	0.0075374945207698435
44-45	0.008727663832157954
46-47	0.0052060062592265656
48-49	0.0010447901538975896
50-51	0.0025205044742019895
52-53	0.0010324479237711563
54-55	0.005201711816106998
56-57	8.336891440513559E-4
58-59	0.0014876655572833234
60-61	8.757732347852125E-4
62-63	0.0013685566294070143
64-65	4.2096504130894864E-4
66-67	0.005840500996482579
68-69	0.0039731534024595805
70-71	0.001699007658169939
72-73	0.0014798947320704466
74-75	7.520064834528205E-4
76-77	6.843588474931061E-4
78-79	7.918190429547167E-4
80-81	0.0011083594241141769
82-83	0.007309564606268552
84-85	7.66613817913435E-4
86-87	6.0705691522814416E-5
88-89	5.522674760248799E-4
90-91	3.573183537193811E-4
92-93	0.0024343990512619047
94-95	0.003373363377901794
96-97	4.1055423261296664E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	133.0
20-21	428.0
22-23	1104.0
24-25	2580.0
26-27	2778.0
28-29	4389.0
30-31	8134.0
32-33	10287.0
34-35	13697.0
36-37	17770.0
38-39	21632.0
40-41	24730.0
42-43	33284.0
44-45	36642.0
46-47	36161.0
48-49	31105.0
50-51	27738.0
52-53	26140.0
54-55	28361.0
56-57	30616.0
58-59	32103.0
60-61	32893.0
62-63	33702.0
64-65	36936.0
66-67	40702.0
68-69	42652.0
70-71	44023.0
72-73	44585.0
74-75	47517.0
76-77	46378.0
78-79	52025.0
80-81	56022.0
82-83	56969.0
84-85	61961.0
86-87	68065.0
88-89	78732.0
90-91	87931.0
92-93	101564.0
94-95	128104.0
96-97	317427.0
98-99	148531.0
100-101	5675210.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.12733698459941
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.79191990191795	74.69827767098211
2	7.831554537063017	13.176956553055378
3	1.902255350500973	4.800950307070579
4	0.6998849959621608	2.355178436230948
5	0.31554378713448317	1.3272929256829684
6	0.15804915628652813	0.7977752778629021
7	0.09223226569736065	0.5431478428022478
8	0.05997931641688528	0.40367201314473794
9	0.03577633217933093	0.2708790797991187
>10	0.1107672131760199	1.3876939412515803
>50	0.001384262660821636	0.07581652758867394
>100	5.770555706118097E-4	0.09944134997337474
>500	5.0550333969806476E-5	0.03246907415214594
>1k	2.527509986663055E-5	0.030449000403207863
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.3172209115142363E-5	0.0	0.0	0.0
3	0.0	1.3172209115142363E-5	0.0	0.0	0.0
4	0.0	3.951662734542709E-5	0.0	0.0	0.0
5	2.6344418230284725E-5	3.951662734542709E-5	0.0	0.0	0.0
6	1.3172209115142365E-4	3.951662734542709E-5	0.0	0.0	0.0
7	1.3172209115142365E-4	3.951662734542709E-5	0.0	0.0	0.0
8	1.3172209115142365E-4	3.951662734542709E-5	0.0	0.0	0.0
9	1.44894300266566E-4	1.053776729211389E-4	0.0	1.3172209115142363E-5	0.0
10-11	1.778248230544219E-4	1.053776729211389E-4	0.0	1.3172209115142363E-5	0.0
12-13	2.0416924128470663E-4	1.053776729211389E-4	0.0	1.3172209115142363E-5	6.586104557571181E-6
14-15	2.2392755495742017E-4	1.2513598659385247E-4	0.0	3.951662734542709E-5	1.9758313672713545E-5
16-17	2.634441823028473E-4	1.3172209115142365E-4	0.0	5.268883646056945E-5	2.6344418230284725E-5
18-19	2.8320249597556084E-4	1.3172209115142365E-4	0.0	7.903325469085418E-5	3.2930522787855905E-5
20-21	3.1613301876341673E-4	1.3172209115142365E-4	0.0	1.053776729211389E-4	6.586104557571182E-5
22-23	3.293052278785591E-4	1.5148040482413716E-4	0.0	1.9099703216956425E-4	6.586104557571182E-5
24-25	3.556496461088438E-4	1.8441092761199308E-4	0.0	4.280967962421268E-4	8.561935924842535E-5
26-27	3.6882185522398616E-4	2.107553458422778E-4	0.0	0.001014260101865962	9.220546380599654E-5
28-29	3.6882185522398616E-4	2.17341450399849E-4	0.0	0.0033062244879007332	9.879156836356773E-5
30-31	3.885801688966997E-4	2.502719731877049E-4	0.0	0.0038989738980821395	1.053776729211389E-4
32-33	3.951662734542709E-4	2.502719731877049E-4	0.0	0.005005439463754098	1.053776729211389E-4
34-35	4.1492458712698445E-4	3.0954691420584553E-4	0.0	0.0065795184530136105	1.053776729211389E-4
36-37	4.34682900799698E-4	3.819940643391285E-4	0.0	0.008799035688915099	1.053776729211389E-4
38-39	4.544412144724115E-4	3.951662734542709E-4	0.0	0.013336861729081642	1.053776729211389E-4
40-41	4.8737173726026744E-4	4.34682900799698E-4	0.0	0.017920790501151185	1.053776729211389E-4
42-43	4.939578418178386E-4	4.412690053572692E-4	0.0	0.022228102881802738	1.1196377747871009E-4
44-45	5.07130050932981E-4	5.137161554905522E-4	0.0	0.02770774187370196	1.3830819570899482E-4
46-47	5.334744691632657E-4	5.79577201066264E-4	0.0	0.03330593074763746	1.5806650938170836E-4
48-49	5.598188873935505E-4	5.79577201066264E-4	0.0	0.038838258575997264	1.5806650938170836E-4
50-51	5.927494101814064E-4	7.2447150133283E-4	0.0	0.04428496704510863	1.5806650938170836E-4
52-53	6.256799329692623E-4	7.376437104479723E-4	0.0	0.05525083113346464	1.6465261393927954E-4
54-55	6.388521420844047E-4	7.442298150055435E-4	0.0	0.0681266655435163	1.712387184968507E-4
56-57	6.454382466419757E-4	7.63988128678257E-4	0.0	0.08344594474442688	1.8441092761199308E-4
58-59	6.586104557571181E-4	7.771603377933994E-4	0.0	0.09706600896948407	1.9758313672713545E-4
60-61	6.651965603146893E-4	8.035047560236841E-4	0.0	0.10345453039032812	1.9758313672713545E-4
62-63	7.2447150133283E-4	8.166769651388265E-4	0.0	0.11152909457791038	2.2392755495742017E-4
64-65	7.376437104479723E-4	8.69365801599396E-4	0.0	0.11537537963953196	2.2392755495742017E-4
66-67	7.376437104479723E-4	8.825380107145383E-4	0.0	0.11837864331778442	2.2392755495742017E-4
68-69	7.63988128678257E-4	9.08882428944823E-4	0.0	0.1215136290871883	2.2392755495742017E-4
70-71	7.96918651466113E-4	9.286407426175366E-4	0.0	0.1239570738780472	2.502719731877049E-4
72-73	8.496074879266824E-4	9.352268471751078E-4	0.0	0.12619634942762142	2.8320249597556084E-4
74-75	8.627796970418248E-4	9.549851608478214E-4	0.0	0.12826438625869876	2.89788600533132E-4
76-77	8.69365801599396E-4	9.747434745205348E-4	0.0	0.13035218140344884	2.963747050907032E-4
78-79	8.69365801599396E-4	9.879156836356772E-4	0.0	0.13182746882434476	3.029608096482744E-4
80-81	8.825380107145383E-4	9.879156836356772E-4	0.0	0.1322358073069142	3.2271912332098793E-4
82-83	8.891241152721095E-4	9.879156836356772E-4	0.0	0.13258487084846546	3.358913324361302E-4
84-85	8.957102198296807E-4	9.879156836356772E-4	0.0	0.13269683462594417	3.424774369937014E-4
86-87	9.088824289448231E-4	0.001014260101865962	0.0	0.13279562619430774	3.490635415512726E-4
88	9.088824289448231E-4	0.0010669489383265313	0.0	0.13281538450798044	3.556496461088438E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9030	0.0	51.064346	1
GTATCAA	16270	0.0	37.75198	1
TCAACGC	19275	0.0	31.085802	4
ATCAACG	19460	0.0	30.813019	3
CAACGCA	19900	0.0	30.10949	5
TATCAAC	20575	0.0	29.573359	2
AACGCAG	20695	0.0	28.995598	6
ACGCAGA	23900	0.0	25.107277	7
CGCAGAG	24240	0.0	24.755112	8
GTACATG	23365	0.0	22.197273	1
GTGGTAT	4530	0.0	22.175095	1
TACATGG	23125	0.0	21.968376	2
TGGTATC	4330	0.0	21.768549	2
ACATGGG	23740	0.0	20.840057	3
GCAGAGT	28635	0.0	20.739252	9
GAGTACT	15865	0.0	20.125315	12-13
CATGGGG	18315	0.0	18.991245	4
AGAGTAC	25795	0.0	18.716972	10-11
CAGAGTA	27840	0.0	18.653496	10-11
GTACTTT	17765	0.0	17.873762	14-15
>>END_MODULE