FastQCFastQC Report
Fri 27 May 2016
SRR2139361_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139361_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8658096
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97900.051.2784921
GTATCAA180800.038.5115241
TCAACGC211100.031.9380424
ATCAACG211250.031.8944663
CAACGCA216550.031.1546345
TATCAAC225300.030.219042
AACGCAG225250.030.0493326
ACGCAGA260200.026.1149277
CGCAGAG263650.025.722968
GTACATG246000.021.2327941
GCAGAGT317000.021.1292389
TACATGG243600.020.9707322
ACATGGG247200.020.0937753
GAGTACT182200.019.81159612-13
GTGGTAT52850.019.3820841
AGAGTAC281200.019.03910310-11
CAGAGTA312100.018.61131710-11
TGGTATC50600.018.411512
CATGGGG180400.018.1603764
GTACTTT201950.017.9293214-15
Produced by FastQC (version 0.11.5)