##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139361_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8658096
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1962526172036	33.0	31.0	34.0	31.0	34.0
2	32.41793426637912	34.0	31.0	34.0	31.0	34.0
3	32.46497428533941	34.0	31.0	34.0	31.0	34.0
4	36.0921780031083	37.0	35.0	37.0	35.0	37.0
5	35.95544909642952	37.0	35.0	37.0	35.0	37.0
6	35.97783658208456	37.0	35.0	37.0	35.0	37.0
7	35.93316879369321	37.0	35.0	37.0	35.0	37.0
8	35.93323751549995	37.0	35.0	37.0	35.0	37.0
9	37.71281965457533	39.0	37.0	39.0	35.0	39.0
10-11	37.600546124690695	39.0	37.0	39.0	35.0	39.0
12-13	37.56527428201304	39.0	37.0	39.0	35.0	39.0
14-15	38.84910487248004	40.0	38.0	41.0	35.0	41.0
16-17	38.83548374838995	40.0	38.0	41.0	35.0	41.0
18-19	38.816405131105036	40.0	38.0	41.0	35.0	41.0
20-21	38.805941005304945	40.0	38.0	41.0	34.5	41.0
22-23	38.7284072672508	40.0	38.0	41.0	34.0	41.0
24-25	38.66545071644549	40.0	38.0	41.0	34.0	41.0
26-27	38.50239567607245	40.0	38.0	41.0	34.0	41.0
28-29	38.37109049853608	40.0	38.0	41.0	34.0	41.0
30-31	38.23807643500942	40.0	38.0	41.0	34.0	41.0
32-33	38.15177281159379	40.0	38.0	41.0	33.5	41.0
34-35	38.209464908515514	40.0	38.0	41.0	34.0	41.0
36-37	38.17249419559721	40.0	38.0	41.0	33.0	41.0
38-39	38.1127916725323	40.0	38.0	41.0	33.0	41.0
40-41	38.042253266509036	40.0	37.5	41.0	33.0	41.0
42-43	38.00974746517431	40.0	37.0	41.0	33.0	41.0
44-45	38.039093425056166	40.0	37.0	41.0	33.0	41.0
46-47	38.02448870048964	40.0	37.0	41.0	33.0	41.0
48-49	37.94365082028836	40.0	37.0	41.0	33.0	41.0
50-51	37.86536546070539	40.0	37.0	41.0	33.0	41.0
52-53	37.638868524701806	40.0	37.0	41.0	32.0	41.0
54-55	37.521329672333906	39.5	36.0	41.0	32.0	41.0
56-57	37.4578078975281	39.0	36.0	41.0	32.0	41.0
58-59	37.299360482805845	39.0	36.0	41.0	31.5	41.0
60-61	37.109503463234944	39.0	35.0	41.0	31.0	41.0
62-63	36.8624183462941	39.0	35.0	40.0	31.0	41.0
64-65	36.568914946500634	38.0	35.0	40.0	31.0	41.0
66-67	36.28595991812139	38.0	35.0	40.0	30.5	41.0
68-69	36.00396781470043	37.0	34.5	40.0	30.0	41.0
70-71	35.70242587394185	37.0	34.0	39.0	30.0	41.0
72-73	35.38883753741895	36.0	34.0	39.0	30.0	40.5
74-75	35.0328044028513	36.0	34.0	39.0	29.0	40.0
76-77	33.68993210011128	34.5	32.0	36.5	28.0	39.0
78-79	34.406527655168816	35.0	33.5	37.0	29.0	39.0
80-81	34.34343413937336	35.0	34.0	37.0	29.0	39.0
82-83	34.0565637892573	35.0	34.0	36.0	29.0	38.5
84-85	33.86438482905929	35.0	33.0	36.0	29.0	37.0
86-87	33.62247399034674	35.0	33.0	35.5	29.0	37.0
88-89	33.50399179635752	35.0	33.0	35.0	29.0	36.5
90-91	33.31725946390405	35.0	33.0	35.0	29.0	36.0
92-93	33.20767609861913	35.0	33.0	35.0	29.0	36.0
94-95	33.19933798019923	35.0	33.0	35.0	29.0	36.0
96-97	33.232762936923194	35.0	33.0	35.0	29.0	35.5
98-99	33.32940466328758	35.0	33.0	35.0	29.5	35.0
100	32.042443253697044	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	6.0
20	29.0
21	222.0
22	1085.0
23	4035.0
24	11249.0
25	25226.0
26	46566.0
27	74702.0
28	108343.0
29	144888.0
30	184291.0
31	228387.0
32	283685.0
33	357365.0
34	475046.0
35	657987.0
36	1022013.0
37	1795969.0
38	2287741.0
39	922068.0
40	27191.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.277324861877815	20.41401717121376	14.62125328955157	23.687404677356856
2	14.44880029050267	21.38395092870303	41.519763698623805	22.64748508217049
3	18.010622658838617	26.295273233283623	30.83815425469988	24.85594985317788
4	12.384789912239365	18.38996703201258	39.42407198996177	29.801171065786285
5	12.924435118298527	38.17118682906727	35.93757796171352	12.966800090920682
6	29.530730543990273	38.467406690801305	19.050666566875673	12.95119619833275
7	26.228087561052682	32.942057930519596	23.385395588129306	17.444458920298413
8	24.31599280026463	36.854650260288174	21.346667904814176	17.48268903463302
9	25.40736439050803	17.053206617251647	21.381248255967595	36.15818073627273
10-11	23.891749634778225	26.997068812650543	29.28088515540167	19.830296397169562
12-13	24.771624153855537	24.493092938678434	29.32918507718094	21.406097830285088
14-15	21.832762076096177	25.968873526003872	26.958796714658746	25.23956768324121
16-17	21.399634060328935	29.13848482865729	27.642992430859632	21.81888868015414
18-19	21.427629700190888	28.3441069727541	29.306940298916878	20.921323028138126
20-21	22.240323322652856	27.506617402720973	29.225039972381143	21.028019302245024
22-23	21.913438148797297	27.431224057133	29.083642994382636	21.571694799687062
24-25	21.687468067686915	27.57104946882886	29.161079354354335	21.580403109129886
26-27	21.69549240484813	27.74702196265158	29.007770531970124	21.54971510053016
28-29	21.618571916123837	27.865378677034503	29.025256197724037	21.490793209117623
30-31	21.93190865442658	27.640228940675886	28.96161678520351	21.46624561969402
32-33	21.74234643985582	27.767000895760148	28.90180222864569	21.588850435738347
34-35	21.87237221365447	27.803495217639163	28.85944833471084	21.46468423399553
36-37	21.810641757149646	27.76687574736542	28.854322042394415	21.568160453090517
38-39	21.687898436178514	27.902974044258645	28.9079877799261	21.50113973963675
40-41	21.97698369923696	27.770003689907274	28.721377866202545	21.531634744653214
42-43	21.846140794509125	27.858233811270395	28.566899307720817	21.728726086499666
44-45	21.940496404411967	27.97650309538049	28.384249462922334	21.69875103728521
46-47	21.973071853123205	27.945341974952353	28.13247975690692	21.949106415017518
48-49	21.930660142250872	28.04171555493533	28.176663846288854	21.85096045652494
50-51	21.912573307810927	28.1276180827239	28.10005058525181	21.859758024213363
52-53	22.068449324048387	28.07052540820583	27.99055830004271	21.87046696770307
54-55	22.055184528765185	27.97090649281488	28.00806405945211	21.965844918967832
56-57	22.030210405018423	28.07881251910434	28.014297080859407	21.876679995017824
58-59	22.01788739547083	28.141076298806052	28.00655079650156	21.83448550922156
60-61	22.062985526276833	28.11949039943374	28.008471976638884	21.80905209765055
62-63	22.020597565423312	28.12691870828985	27.985867388942324	21.866616337344507
64-65	21.990081052247824	28.124474089997697	27.93913801123692	21.94630684651756
66-67	22.044549606200317	28.1416053408495	27.8522789497956	21.96156610315458
68-69	22.026132960161547	28.11531262689767	27.926115628329434	21.932438784611357
70-71	21.981394399516173	27.922760811927304	27.969682142504777	22.126162646051743
72-73	22.008733481001375	27.974695875305876	27.951953093264738	22.06461755042801
74-75	21.938600552533973	28.082838121791514	27.919233880609923	22.05932744506459
76-77	22.034810080780527	28.00843706864122	27.854776161899704	22.101976688678544
78-79	22.01420197846125	28.018365537124033	27.811387476798426	22.15604500761629
80-81	22.003158369249316	28.011544877482482	27.847286005656557	22.13801074761165
82-83	22.033147710213854	27.968977344814576	27.962070915972177	22.035804028999394
84-85	22.138612438548385	27.970434547757865	27.875999718673793	22.01495329501995
86-87	21.953415200691005	27.990195852464524	27.959958736959745	22.096430209884733
88-89	22.026545632367096	27.951131194950552	27.932917431004313	22.089405741678043
90-91	22.05600459038546	27.948738871759147	27.95587971455507	22.039376823300323
92-93	22.044378350878567	27.866937942637925	28.047913487000876	22.040770219482635
94-95	21.916545271135348	27.977688534046806	28.062346655954784	22.04341953886306
96-97	21.93415989269329	28.097000035357244	28.150549270104264	21.8182908018452
98-99	22.05519974233508	28.902184767346302	27.269777859696713	21.772837630621904
100	20.27229110606558	28.798625709323943	28.268090851129823	22.66099233348065
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1210.0
1	1069.0
2	1613.0
3	2446.5
4	3282.5
5	5195.5
6	6890.5
7	8347.5
8	9660.0
9	10030.0
10	9385.5
11	8420.0
12	8945.5
13	11746.5
14	18383.0
15	28076.5
16	36530.5
17	42093.0
18	44163.5
19	42722.0
20	39445.5
21	35988.0
22	34293.5
23	36183.0
24	41803.0
25	51290.0
26	65677.5
27	82567.5
28	101024.5
29	121369.5
30	140865.5
31	161029.5
32	183707.5
33	205723.5
34	226857.5
35	245904.0
36	265143.5
37	284243.5
38	297186.5
39	307451.5
40	319772.5
41	331333.5
42	337693.5
43	342655.5
44	346413.5
45	348660.5
46	349252.0
47	345495.0
48	339596.0
49	331070.5
50	320665.5
51	308730.5
52	292605.0
53	273182.0
54	254084.0
55	233982.5
56	213042.5
57	191207.0
58	168929.0
59	146392.5
60	121599.0
61	98404.0
62	77360.5
63	58744.5
64	43811.0
65	32566.5
66	24599.0
67	18841.0
68	14287.5
69	10418.0
70	7595.0
71	5548.5
72	3953.0
73	2680.0
74	1772.0
75	1137.5
76	740.5
77	489.0
78	329.0
79	212.5
80	136.0
81	92.5
82	68.0
83	43.5
84	28.5
85	20.0
86	13.0
87	9.5
88	7.0
89	5.0
90	3.0
91	2.0
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.1549883484775405E-5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	5.774941742387703E-6
12-13	0.0
14-15	0.0
16-17	0.0012878120085524577
18-19	4.042459219671392E-5
20-21	0.0
22-23	0.006884412390856507
24-25	0.008323949875380477
26-27	0.02151837067334992
28-29	0.016232042363780765
30-31	0.0019610011819226002
32-33	0.006069048046070561
34-35	0.03325561703262925
36-37	0.005637160393775994
38-39	0.0024471712535687183
40-41	0.00693745617333658
42-43	0.007391775171766376
44-45	0.008304665341731675
46-47	0.005047595208931637
48-49	8.572985997218067E-4
50-51	0.002666401222240058
52-53	8.581762350716342E-4
54-55	0.00495771096641449
56-57	7.13808473692982E-4
58-59	0.0012820468418598865
60-61	7.87488384546328E-4
62-63	0.0013432694380748188
64-65	4.1918511646257074E-4
66-67	0.005238113480722069
68-69	0.0038977951949976503
70-71	0.0015941449941399481
72-73	0.0013519137666010588
74-75	7.94960838639167E-4
76-77	6.404097802869317E-4
78-79	7.805563289442414E-4
80-81	9.884437914935047E-4
82-83	0.007004320380237533
84-85	6.351633160368217E-4
86-87	7.348829565276647E-5
88-89	3.78185733632037E-4
90-91	3.4199321718012503E-4
92-93	0.00237992233682007
94-95	0.0031826940481857057
96-97	7.953566212787975E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	145.0
20-21	479.0
22-23	1161.0
24-25	2474.0
26-27	3203.0
28-29	5003.0
30-31	8896.0
32-33	12063.0
34-35	16148.0
36-37	21007.0
38-39	25407.0
40-41	29532.0
42-43	38894.0
44-45	43099.0
46-47	42608.0
48-49	36394.0
50-51	32236.0
52-53	30992.0
54-55	33721.0
56-57	36229.0
58-59	37943.0
60-61	38780.0
62-63	40149.0
64-65	43799.0
66-67	48085.0
68-69	50051.0
70-71	52021.0
72-73	52109.0
74-75	54837.0
76-77	54552.0
78-79	61438.0
80-81	64424.0
82-83	66133.0
84-85	70814.0
86-87	77911.0
88-89	89782.0
90-91	101674.0
92-93	115640.0
94-95	144767.0
96-97	357480.0
98-99	164979.0
100-101	6451037.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.67081221683698
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.35470364602581	73.92709817240906
2	8.111896678756224	13.574579674611911
3	2.018963277646109	5.067848918298519
4	0.7330048197058818	2.453244344945891
5	0.31429149035329723	1.3148512135350283
6	0.16747730979591513	0.8407777523109027
7	0.09134079894364679	0.5349791185304812
8	0.05741564730796482	0.38432110753702925
9	0.0429953709250812	0.3237711846179149
>10	0.10562475800717677	1.299018709615441
>50	0.0015820943107000037	0.0866682200533221
>100	5.705008387660755E-4	0.08535544247548044
>500	6.680417323808439E-5	0.034114915263215626
>1k	6.68032102161245E-5	0.07337122579573897
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	1.1549883484775405E-5	0.0	0.0	0.0
4	0.0	2.309976696955081E-5	0.0	0.0	1.1549883484775405E-5
5	0.0	2.309976696955081E-5	0.0	0.0	1.1549883484775405E-5
6	0.0	2.309976696955081E-5	0.0	0.0	1.1549883484775405E-5
7	0.0	3.464965045432622E-5	0.0	1.1549883484775405E-5	1.1549883484775405E-5
8	0.0	4.619953393910162E-5	0.0	1.1549883484775405E-5	1.1549883484775405E-5
9	0.0	1.3859860181730487E-4	0.0	1.1549883484775405E-5	2.309976696955081E-5
10-11	0.0	1.3859860181730487E-4	0.0	2.309976696955081E-5	2.8874708711938516E-5
12-13	0.0	1.4437354355969259E-4	0.0	2.309976696955081E-5	3.464965045432622E-5
14-15	0.0	2.13672844468345E-4	0.0	2.8874708711938516E-5	6.352435916626473E-5
16-17	0.0	2.194477862107327E-4	0.0	3.464965045432622E-5	6.929930090865243E-5
18-19	0.0	2.2522272795312038E-4	0.0	3.464965045432622E-5	7.507424265104014E-5
20-21	0.0	2.309976696955081E-4	0.0	6.929930090865243E-5	1.0394895136297865E-4
22-23	0.0	2.598723784074466E-4	0.0	1.5592342704446796E-4	1.0972389310536635E-4
24-25	0.0	2.656473201498343E-4	0.0	4.100208637095269E-4	1.1549883484775405E-4
26-27	0.0	2.656473201498343E-4	0.0	0.0011376635232503774	1.2704871833252947E-4
28-29	0.0	2.656473201498343E-4	0.0	0.0036093385889923143	1.3282366007491715E-4
30-31	0.0	3.0029697060416055E-4	0.0	0.003932735326566026	1.4437354355969259E-4
32-33	0.0	3.0029697060416055E-4	0.0	0.0046430531608797135	1.6169836878685568E-4
34-35	0.0	3.1762179583132367E-4	0.0	0.005422670296102053	1.6169836878685568E-4
36-37	0.0	3.407215628008745E-4	0.0	0.0067509068968512245	1.7324825227163108E-4
38-39	0.0	3.4649650454326216E-4	0.0	0.010625892805993374	1.7324825227163108E-4
40-41	0.0	3.753712132552007E-4	0.0	0.014229456453243298	1.7324825227163108E-4
42-43	0.0	3.9847098022475146E-4	0.0	0.01745187394549564	1.7902319401401877E-4
44-45	0.0	4.331206306790777E-4	0.0	0.02115938654410854	1.8479813575640648E-4
46-47	0.0	4.619953393910162E-4	0.0	0.025432843433475443	1.8479813575640648E-4
48-49	0.0	4.619953393910162E-4	0.0	0.029775599623750995	1.963480192411819E-4
50-51	0.0	5.717192324963825E-4	0.0	0.03426850429932863	1.963480192411819E-4
52-53	0.0	5.774941742387702E-4	0.0	0.04429380316411368	2.194477862107327E-4
54-55	0.0	5.890440577235457E-4	0.0	0.05654822954146038	2.4254755318028353E-4
56-57	0.0	6.005939412083211E-4	0.0	0.07040808972319088	2.4254755318028353E-4
58-59	0.0	6.005939412083211E-4	0.0	0.0828877388284907	2.4254755318028353E-4
60-61	0.0	6.179187664354842E-4	0.0	0.08921707497814763	2.5409743666505893E-4
62-63	0.0	6.23693708177872E-4	0.0	0.09623362919514868	2.7719720363460974E-4
64-65	0.0	6.929930090865243E-4	0.0	0.09969281929883891	2.7719720363460974E-4
66-67	0.0	6.98767950828912E-4	0.0	0.10301918574245422	2.7719720363460974E-4
68-69	0.0	7.103178343136874E-4	0.0	0.10632245241909999	2.8297214537699743E-4
70-71	0.0	7.160927760560752E-4	0.0	0.10914639893112758	2.9452202886177286E-4
72-73	0.0	7.160927760560752E-4	0.0	0.11135820161846208	3.11846854088936E-4
74-75	0.0	7.218677177984629E-4	0.0	0.11336788134481299	3.11846854088936E-4
76-77	0.0	7.738421934799522E-4	0.0	0.115244737411089	3.11846854088936E-4
78-79	0.0	8.200417274190538E-4	0.0	0.11661917354577728	3.11846854088936E-4
80-81	0.0	8.258166691614415E-4	0.0	0.11712736841910738	3.11846854088936E-4
82-83	0.0	8.315916109038292E-4	0.0	0.11743921527319633	3.2339673757371135E-4
84-85	0.0	8.315916109038292E-4	0.0	0.11752006445758975	3.291716793160991E-4
86-87	0.0	8.315916109038292E-4	0.0	0.11754893916630169	3.4649650454326216E-4
88	0.0	8.777911448429308E-4	0.0	0.11755471410804408	3.4649650454326216E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9790	0.0	51.278492	1
GTATCAA	18080	0.0	38.511524	1
TCAACGC	21110	0.0	31.938042	4
ATCAACG	21125	0.0	31.894466	3
CAACGCA	21655	0.0	31.154634	5
TATCAAC	22530	0.0	30.21904	2
AACGCAG	22525	0.0	30.049332	6
ACGCAGA	26020	0.0	26.114927	7
CGCAGAG	26365	0.0	25.72296	8
GTACATG	24600	0.0	21.232794	1
GCAGAGT	31700	0.0	21.129238	9
TACATGG	24360	0.0	20.970732	2
ACATGGG	24720	0.0	20.093775	3
GAGTACT	18220	0.0	19.811596	12-13
GTGGTAT	5285	0.0	19.382084	1
AGAGTAC	28120	0.0	19.039103	10-11
CAGAGTA	31210	0.0	18.611317	10-11
TGGTATC	5060	0.0	18.41151	2
CATGGGG	18040	0.0	18.160376	4
GTACTTT	20195	0.0	17.92932	14-15
>>END_MODULE