FastQCFastQC Report
Fri 27 May 2016
SRR2139360_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139360_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7895135
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92100.052.3564641
GTATCAA161950.037.8853151
ATCAACG199050.030.071163
TCAACGC199350.030.0037964
CAACGCA203800.029.348665
TATCAAC210700.028.6804182
AACGCAG211800.028.1984946
ACGCAGA244250.024.4521647
CGCAGAG248250.024.0404198
GTGGTAT46350.020.8260541
GTACATG225600.020.6708891
TACATGG218700.020.6579722
GCAGAGT293950.020.1529399
ACATGGG219600.019.8507333
GAGTACT175550.019.78533712-13
TGGTATC45450.019.2988642
CATGGGG165050.018.8539244
AGAGTAC265150.018.5270610-11
CAGAGTA287700.018.5227110-11
TATACCG5250.018.4703875
Produced by FastQC (version 0.11.5)