##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2139360_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7895135 Sequences flagged as poor quality 0 Sequence length 19-100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18660934866852 33.0 31.0 34.0 31.0 34.0 2 32.410562200646346 34.0 31.0 34.0 31.0 34.0 3 32.456041473641676 34.0 31.0 34.0 31.0 34.0 4 36.083543599951106 37.0 35.0 37.0 35.0 37.0 5 35.943058478417406 37.0 35.0 37.0 35.0 37.0 6 35.96521174115452 37.0 35.0 37.0 35.0 37.0 7 35.921990187628204 37.0 35.0 37.0 35.0 37.0 8 35.920296739701094 37.0 35.0 37.0 35.0 37.0 9 37.700280742507886 39.0 37.0 39.0 35.0 39.0 10-11 37.58620644232176 39.0 37.0 39.0 35.0 39.0 12-13 37.552678263259594 39.0 37.0 39.0 35.0 39.0 14-15 38.82812130508218 40.0 38.0 41.0 35.0 41.0 16-17 38.812407957558676 40.0 38.0 41.0 35.0 41.0 18-19 38.7966789041606 40.0 38.0 41.0 35.0 41.0 20-21 38.785696754689596 40.0 38.0 41.0 34.0 41.0 22-23 38.708482182842786 40.0 38.0 41.0 34.0 41.0 24-25 38.64321708432729 40.0 38.0 41.0 34.0 41.0 26-27 38.46972662807093 40.0 38.0 41.0 34.0 41.0 28-29 38.33295762383753 40.0 38.0 41.0 34.0 41.0 30-31 38.200609891246735 40.0 38.0 41.0 34.0 41.0 32-33 38.111682257767484 40.0 37.5 41.0 33.0 41.0 34-35 38.175262048523734 40.0 38.0 41.0 33.5 41.0 36-37 38.13543282505347 40.0 38.0 41.0 33.0 41.0 38-39 38.07017753396158 40.0 37.5 41.0 33.0 41.0 40-41 37.99685184659313 40.0 37.0 41.0 33.0 41.0 42-43 37.96652766299262 40.0 37.0 41.0 33.0 41.0 44-45 37.99354965580814 40.0 37.0 41.0 33.0 41.0 46-47 37.9761495623585 40.0 37.0 41.0 33.0 41.0 48-49 37.89331716328043 40.0 37.0 41.0 33.0 41.0 50-51 37.80572632026795 40.0 37.0 41.0 33.0 41.0 52-53 37.577449085397305 40.0 36.5 41.0 32.0 41.0 54-55 37.45752838396601 39.5 36.0 41.0 32.0 41.0 56-57 37.394415677741776 39.0 36.0 41.0 32.0 41.0 58-59 37.233718273358306 39.0 36.0 41.0 31.0 41.0 60-61 37.04060187791471 39.0 35.0 41.0 31.0 41.0 62-63 36.79308538379848 38.5 35.0 40.0 31.0 41.0 64-65 36.49851822119401 38.0 35.0 40.0 31.0 41.0 66-67 36.21624377728215 37.5 35.0 40.0 30.0 41.0 68-69 35.93064248617321 37.0 34.0 40.0 30.0 41.0 70-71 35.62754591780204 36.5 34.0 39.0 30.0 41.0 72-73 35.31686993234737 36.0 34.0 39.0 29.5 40.5 74-75 34.966327751281355 36.0 34.0 38.5 29.0 40.0 76-77 33.63500890984222 34.5 32.0 36.5 28.0 39.0 78-79 34.35215720772792 35.0 33.5 37.0 29.0 39.0 80-81 34.294800484528 35.0 34.0 37.0 29.0 39.0 82-83 34.01274181947541 35.0 33.5 36.0 29.0 38.5 84-85 33.82460146450909 35.0 33.0 36.0 29.0 37.0 86-87 33.58706951778554 35.0 33.0 35.5 29.0 37.0 88-89 33.46820815732227 35.0 33.0 35.0 29.0 36.5 90-91 33.284977072038615 35.0 33.0 35.0 29.0 36.0 92-93 33.177857931936074 35.0 33.0 35.0 29.0 36.0 94-95 33.17257945925409 35.0 33.0 35.0 29.0 36.0 96-97 33.21288294760264 35.0 33.0 35.0 29.0 35.5 98-99 33.31322945239309 35.0 33.0 35.0 29.5 35.0 100 31.99417028909493 34.0 31.0 35.0 26.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 4.0 20 31.0 21 215.0 22 1091.0 23 4125.0 24 11104.0 25 24748.0 26 45080.0 27 71090.0 28 102146.0 29 136234.0 30 171563.0 31 212476.0 32 261720.0 33 329203.0 34 437140.0 35 605266.0 36 940544.0 37 1639595.0 38 2050305.0 39 826562.0 40 24892.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.25434207268147 20.326555530716067 14.756163130839434 23.66293926576303 2 14.539143409200731 21.30648810944968 41.36344723681103 22.790921244538566 3 18.021794434167372 26.13396731024865 30.87160637531847 24.972631880265507 4 12.431149055715956 18.355455606522245 39.1643081467258 30.049087191036 5 13.028998237522222 37.999856873884994 35.796753317074376 13.17439157151841 6 29.685230208223167 38.35733017864799 18.87262219075418 13.084817422374664 7 26.368098835548725 32.933027744300766 23.174714555229265 17.524158864921247 8 24.490233542555004 36.67985411269092 21.184970238000997 17.644942106753085 9 25.503781759273274 16.96867754636241 21.25054226431847 36.27699843004584 10-11 23.95132572147278 26.903092854017064 29.087381026416903 20.058200398093256 12-13 24.84631358425157 24.453407066503612 29.17084381711016 21.529435532134663 14-15 21.971175920361084 25.916282622146426 26.841035650435362 25.27150580705713 16-17 21.491218820528868 29.11303092442171 27.540765000702656 21.854985254346765 18-19 21.479991908937293 28.22927193988295 29.263003995753838 21.02773215542592 20-21 22.354076757570763 27.395707770926165 29.19059536633512 21.05962010516795 22-23 22.000137451254687 27.32902124888717 29.063003664627807 21.60783763523034 24-25 21.821417855452193 27.456295469589293 29.104902276811917 21.617384398146598 26-27 21.72813944591086 27.635464027408073 29.015768314242617 21.620628212438444 28-29 21.645557949980514 27.70341364287909 29.05851754504215 21.592510862098244 30-31 21.98340781748257 27.498885101112396 28.96190398907618 21.555803092328855 32-33 21.78664708777545 27.6296324435 28.93854730149502 21.64517316722953 34-35 21.908296269595922 27.694401339182072 28.905018572598085 21.492283818623918 36-37 21.91571421567337 27.608730260790942 28.796575059186623 21.678980464349067 38-39 21.76224083255267 27.76937413433984 28.87169121722446 21.59669381588303 40-41 21.985372394410856 27.63271150713567 28.746190667585324 21.63572543086815 42-43 21.8693708208591 27.73378321591924 28.60939449660834 21.787451466613327 44-45 21.99506446398542 27.819649132069262 28.386675476149435 21.798610927795885 46-47 22.037518926918597 27.808428200186714 28.124111543008844 22.02994132988584 48-49 21.967187372623943 27.92411294620884 28.212324814716972 21.89637486645024 50-51 21.988332910003603 28.103527890775148 27.988493398814168 21.91964580040708 52-53 22.11273721184532 28.044053586034057 27.87819764026619 21.96501156185444 54-55 22.08411770963642 27.928516384039643 27.95972924842666 22.027636657897276 56-57 22.07639397102151 28.045423154663563 27.92702742872001 21.95115544559492 58-59 22.087278917705447 28.033676945314724 27.959830559561432 21.9192135774184 60-61 22.119201491641878 28.033476399402975 27.976080765889265 21.87124134306588 62-63 22.051336448211558 28.105814360626407 27.908841936968148 21.934007254193887 64-65 22.05826987416185 28.021032189007556 27.917886944048792 22.0028109927818 66-67 22.099670580550534 28.06199003781233 27.79836170900843 22.039977672628705 68-69 22.085601818164598 28.044217077783856 27.850789324967824 22.019391779083723 70-71 22.035487341151757 27.86081570333117 27.923469760989622 22.180227194527447 72-73 22.063876019102143 27.882177036653605 27.90057657448169 22.153370369762566 74-75 21.955903407143193 28.01226034947471 27.84698774665212 22.184848496729977 76-77 22.075084779359297 27.94682799924943 27.78834378388944 22.18974343750183 78-79 22.056135393718655 27.97171123500651 27.76053859926343 22.21161477201141 80-81 22.04001612568119 27.979532216304996 27.79956142100511 22.180890237008697 82-83 22.09730697894145 27.89097845422769 27.88802362361787 22.123690943212996 84-85 22.199913987273032 27.89207589529773 27.830992280722217 22.077017836707025 86-87 22.015285220044788 27.912446280783676 27.91670104956607 22.155567449605464 88-89 22.091484259587048 27.869122196340225 27.877271045071616 22.162122499001118 90-91 22.08632296291829 27.86672747695114 27.936307509065006 22.11064205106556 92-93 22.089768596669522 27.81410397211486 27.983361365720782 22.11276606549483 94-95 21.944646158794672 27.910629144140604 28.02394709194043 22.1207776051243 96-97 21.95688138785172 28.03181878425335 28.116786791466104 21.89451303642883 98-99 22.089417204348106 28.805908584645834 27.234736772165256 21.869937438840807 100 20.309510127683108 28.718928429761625 28.267791938170532 22.703769504384734 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1287.0 1 1138.0 2 1602.5 3 2400.0 4 3222.5 5 5096.5 6 6863.5 7 8392.5 8 9820.5 9 10113.0 10 9228.0 11 8335.5 12 8880.5 13 11524.5 14 18038.5 15 27584.5 16 35619.5 17 40527.0 18 42421.5 19 41089.5 20 37750.0 21 34353.0 22 32589.0 23 34068.5 24 39207.0 25 48240.5 26 61254.0 27 76057.0 28 92299.0 29 110482.5 30 127943.5 31 146018.0 32 165480.0 33 184070.0 34 201612.5 35 217348.0 36 234628.5 37 251522.0 38 263526.5 39 272200.5 40 282618.5 41 293647.5 42 300364.0 43 304894.5 44 308771.5 45 313255.0 46 315588.5 47 314556.5 48 311690.0 49 305637.5 50 297292.5 51 286693.5 52 273143.5 53 257435.0 54 240529.0 55 221885.0 56 201819.0 57 181006.0 58 159864.0 59 138388.5 60 115607.0 61 93640.5 62 73786.0 63 56955.5 64 43158.5 65 31790.0 66 23743.0 67 18339.0 68 14022.0 69 10213.5 70 7325.0 71 5350.5 72 3871.5 73 2655.5 74 1777.5 75 1164.5 76 754.5 77 470.5 78 307.0 79 208.5 80 142.5 81 97.0 82 68.0 83 43.0 84 24.5 85 16.5 86 12.5 87 10.5 88 8.0 89 5.5 90 4.5 91 4.5 92 5.0 93 5.5 94 4.0 95 3.0 96 3.0 97 2.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0012222716900977628 18-19 5.066411150664302E-5 20-21 0.0 22-23 0.00697975623676372 24-25 0.008172074235882551 26-27 0.021178307279114753 28-29 0.01602062101810381 30-31 0.0019921540851244902 32-33 0.006237650943848056 34-35 0.03285272335334949 36-37 0.005514621269199329 38-39 0.002614572116323528 40-41 0.007105567115640603 42-43 0.007158739446411847 44-45 0.008274380260886874 46-47 0.005118681209184696 48-49 0.0010459640398870543 50-51 0.0023508037450536267 52-53 8.834320623602826E-4 54-55 0.005050734395315884 56-57 8.77785814542344E-4 58-59 0.0012427847492137215 60-61 8.258779384636333E-4 62-63 0.001349523931816383 64-65 4.205009400569806E-4 66-67 0.005272561413062829 68-69 0.003788385715315167 70-71 0.0016992983762789552 72-73 0.0014602594561187166 74-75 6.791499087593605E-4 76-77 7.756675624216708E-4 78-79 7.743007726598233E-4 80-81 0.001081431660824071 82-83 0.007079532990659857 84-85 7.216269933396745E-4 86-87 5.152831510359694E-5 88-89 4.465102655314689E-4 90-91 3.0907611044450346E-4 92-93 0.002317283531876606 94-95 0.003532307817751901 96-97 5.5821278130235985E-5 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 143.0 20-21 496.0 22-23 1193.0 24-25 2395.0 26-27 3118.0 28-29 4866.0 30-31 8659.0 32-33 11527.0 34-35 15325.0 36-37 19978.0 38-39 24109.0 40-41 27694.0 42-43 36689.0 44-45 41131.0 46-47 40215.0 48-49 35229.0 50-51 30991.0 52-53 29979.0 54-55 33252.0 56-57 36013.0 58-59 36285.0 60-61 36631.0 62-63 37277.0 64-65 40556.0 66-67 44524.0 68-69 46541.0 70-71 47895.0 72-73 48569.0 74-75 51150.0 76-77 50080.0 78-79 56377.0 80-81 59750.0 82-83 61286.0 84-85 65309.0 86-87 71230.0 88-89 82834.0 90-91 92369.0 92-93 106584.0 94-95 133448.0 96-97 325658.0 98-99 154094.0 100-101 5843686.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.97259329010663 #Duplication Level Percentage of deduplicated Percentage of total 1 88.63688588591451 74.4306916899949 2 7.941199408357385 13.336862163072599 3 1.950725122675696 4.914223420417187 4 0.6987518982700209 2.347040358564737 5 0.2992404863600145 1.2563999828521597 6 0.16905828014095184 0.8517757320360635 7 0.08987983411336181 0.5283209928488507 8 0.05597790216575407 0.37604876894385963 9 0.04001191391972083 0.3023913756905526 >10 0.11627795648230202 1.4377634932250065 >50 0.0014796664724645422 0.08211671701149083 >100 4.385607313805552E-4 0.0700849875572106 >500 2.4361770236828717E-5 0.010678663744884544 >1k 4.8722626175471574E-5 0.05560165404043879 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.2666027876660755E-5 0.0 0.0 0.0 3 0.0 1.2666027876660755E-5 0.0 2.533205575332151E-5 0.0 4 0.0 2.533205575332151E-5 0.0 2.533205575332151E-5 1.2666027876660755E-5 5 0.0 2.533205575332151E-5 0.0 3.7998083629982264E-5 1.2666027876660755E-5 6 1.2666027876660755E-5 2.533205575332151E-5 0.0 6.333013938330377E-5 2.533205575332151E-5 7 1.2666027876660755E-5 2.533205575332151E-5 0.0 7.599616725996453E-5 2.533205575332151E-5 8 1.2666027876660755E-5 2.533205575332151E-5 0.0 7.599616725996453E-5 2.533205575332151E-5 9 1.2666027876660755E-5 3.7998083629982264E-5 0.0 8.866219513662528E-5 3.7998083629982264E-5 10-11 2.533205575332151E-5 3.7998083629982264E-5 0.0 8.866219513662528E-5 3.7998083629982264E-5 12-13 3.7998083629982264E-5 3.7998083629982264E-5 0.0 9.499520907495565E-5 3.7998083629982264E-5 14-15 3.7998083629982264E-5 7.599616725996453E-5 0.0 1.1399425088994678E-4 3.7998083629982264E-5 16-17 7.599616725996453E-5 7.599616725996453E-5 0.0 1.4565932058159867E-4 3.7998083629982264E-5 18-19 7.599616725996453E-5 7.599616725996453E-5 0.0 1.7732439027325056E-4 5.066411150664302E-5 20-21 8.866219513662528E-5 7.599616725996453E-5 0.0 1.8365740421158092E-4 5.066411150664302E-5 22-23 1.0132822301328604E-4 8.232918119829491E-5 0.0 2.4698754359488465E-4 5.6997125444973396E-5 24-25 1.2666027876660755E-4 9.499520907495565E-5 0.0 5.953033102030555E-4 6.966315332163414E-5 26-27 1.5199233451992905E-4 1.0132822301328604E-4 0.0 0.0012666027876660753 7.599616725996453E-5 28-29 1.646583623965898E-4 1.0132822301328604E-4 0.0 0.003958133711456486 8.232918119829491E-5 30-31 1.7732439027325056E-4 1.1399425088994678E-4 0.0 0.00437611263138629 9.499520907495565E-5 32-33 1.8365740421158092E-4 1.1399425088994678E-4 0.0 0.005250068554875882 1.1399425088994678E-4 34-35 2.0265644602657207E-4 1.899904181499113E-4 0.0 0.006497672300726967 1.1399425088994678E-4 36-37 2.1532247390323281E-4 2.533205575332151E-4 0.0 0.008631897997944303 1.1399425088994678E-4 38-39 2.1532247390323281E-4 2.659865854098758E-4 0.0 0.013058674740837238 1.2666027876660755E-4 40-41 2.2798850177989356E-4 3.293167247931796E-4 0.0 0.017751438069140046 1.393263066432683E-4 42-43 2.2798850177989356E-4 3.293167247931796E-4 0.0 0.021937560282376423 1.393263066432683E-4 44-45 2.3432151571822394E-4 3.6098179448483146E-4 0.0 0.02703563650273238 1.393263066432683E-4 46-47 2.4065452965655432E-4 3.926468641764834E-4 0.0 0.03236170122486823 1.5199233451992905E-4 48-49 2.4065452965655432E-4 3.926468641764834E-4 0.0 0.03768776594700407 1.899904181499113E-4 50-51 2.659865854098758E-4 5.003081011280998E-4 0.0 0.043279817254549796 1.899904181499113E-4 52-53 2.723195993482062E-4 5.066411150664302E-4 0.0 0.05536954086282248 1.899904181499113E-4 54-55 2.786526132865366E-4 5.066411150664302E-4 0.0 0.0681748950461265 1.899904181499113E-4 56-57 2.786526132865366E-4 5.066411150664302E-4 0.0 0.08298781464788126 1.899904181499113E-4 58-59 2.786526132865366E-4 5.193071429430908E-4 0.0 0.09664812571285988 1.899904181499113E-4 60-61 2.976516551015277E-4 5.573052265730731E-4 0.0 0.10386776160255651 2.0265644602657207E-4 62-63 3.166506969165188E-4 5.699712544497339E-4 0.0 0.11150537641218294 2.1532247390323281E-4 64-65 3.166506969165188E-4 6.143023520180466E-4 0.0 0.11564716752785101 2.1532247390323281E-4 66-67 3.166506969165188E-4 6.333013938330376E-4 0.0 0.11884533956670786 2.2165548784156317E-4 68-69 3.166506969165188E-4 6.776324914013503E-4 0.0 0.12215117284251631 2.2798850177989356E-4 70-71 3.166506969165188E-4 6.902985192780111E-4 0.0 0.1252733487141132 2.469875435948847E-4 72-73 3.166506969165188E-4 6.966315332163415E-4 0.0 0.1275025696204055 3.039846690398581E-4 74-75 3.166506969165188E-4 7.029645471546719E-4 0.0 0.12934547667645963 3.039846690398581E-4 76-77 3.166506969165188E-4 7.282966029079933E-4 0.0 0.13141003922035532 3.039846690398581E-4 78-79 3.166506969165188E-4 7.472956447229845E-4 0.0 0.13278430324497303 3.039846690398581E-4 80-81 3.293167247931796E-4 7.472956447229845E-4 0.0 0.13328461134610112 3.2298371085484926E-4 82-83 3.4198275266984036E-4 7.472956447229845E-4 0.0 0.1336582591684626 3.5464878054650113E-4 84-85 3.5464878054650113E-4 7.472956447229845E-4 0.0 0.13379125246116755 3.5464878054650113E-4 86-87 3.5464878054650113E-4 7.599616725996452E-4 0.0 0.13386091561448918 3.5464878054650113E-4 88 3.6731480842316184E-4 7.72627700476306E-4 0.0 0.1338672486284275 3.6731480842316184E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9210 0.0 52.356464 1 GTATCAA 16195 0.0 37.885315 1 ATCAACG 19905 0.0 30.07116 3 TCAACGC 19935 0.0 30.003796 4 CAACGCA 20380 0.0 29.34866 5 TATCAAC 21070 0.0 28.680418 2 AACGCAG 21180 0.0 28.198494 6 ACGCAGA 24425 0.0 24.452164 7 CGCAGAG 24825 0.0 24.040419 8 GTGGTAT 4635 0.0 20.826054 1 GTACATG 22560 0.0 20.670889 1 TACATGG 21870 0.0 20.657972 2 GCAGAGT 29395 0.0 20.152939 9 ACATGGG 21960 0.0 19.850733 3 GAGTACT 17555 0.0 19.785337 12-13 TGGTATC 4545 0.0 19.298864 2 CATGGGG 16505 0.0 18.853924 4 AGAGTAC 26515 0.0 18.52706 10-11 CAGAGTA 28770 0.0 18.52271 10-11 TATACCG 525 0.0 18.470387 5 >>END_MODULE