FastQCFastQC Report
Fri 27 May 2016
SRR2139359_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139359_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6988775
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81650.049.0185781
GTATCAA145700.037.5983161
TCAACGC171950.031.1200084
ATCAACG173350.030.9444583
CAACGCA177550.030.2124615
AACGCAG181850.029.618466
TATCAAC183850.029.367712
ACGCAGA210400.025.5994157
CGCAGAG214400.025.1013898
TACATGG200000.021.4349942
GTACATG203150.021.4259571
GCAGAGT259450.020.675369
ACATGGG204200.020.329343
GTGGTAT40550.020.3019471
GAGTACT144800.020.03551112-13
AGAGTAC228350.018.62092810-11
CAGAGTA249750.018.52452710-11
CATGGGG153350.018.3610674
TGGTATC40800.017.8163452
GTACTTT164000.017.70419114-15
Produced by FastQC (version 0.11.5)