##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139359_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6988775
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.195332086095206	33.0	31.0	34.0	31.0	34.0
2	32.41291971769015	34.0	31.0	34.0	31.0	34.0
3	32.4610752241988	34.0	31.0	34.0	31.0	34.0
4	36.087402012512925	37.0	35.0	37.0	35.0	37.0
5	35.947236389782184	37.0	35.0	37.0	35.0	37.0
6	35.97096544101076	37.0	35.0	37.0	35.0	37.0
7	35.92454600412805	37.0	35.0	37.0	35.0	37.0
8	35.92674452962071	37.0	35.0	37.0	35.0	37.0
9	37.703749655697884	39.0	37.0	39.0	35.0	39.0
10-11	37.590944764998156	39.0	37.0	39.0	35.0	39.0
12-13	37.554905616506474	39.0	37.0	39.0	35.0	39.0
14-15	38.833016086510156	40.0	38.0	41.0	35.0	41.0
16-17	38.81685023484087	40.0	38.0	41.0	35.0	41.0
18-19	38.80083312168441	40.0	38.0	41.0	35.0	41.0
20-21	38.78985893662774	40.0	38.0	41.0	34.5	41.0
22-23	38.711035049087045	40.0	38.0	41.0	34.0	41.0
24-25	38.64688790796437	40.0	38.0	41.0	34.0	41.0
26-27	38.474179035423205	40.0	38.0	41.0	34.0	41.0
28-29	38.33981663973526	40.0	38.0	41.0	34.0	41.0
30-31	38.20597754139909	40.0	38.0	41.0	34.0	41.0
32-33	38.121251137584494	40.0	37.5	41.0	33.0	41.0
34-35	38.18100734217123	40.0	38.0	41.0	33.5	41.0
36-37	38.14193781127082	40.0	38.0	41.0	33.0	41.0
38-39	38.07736564969365	40.0	37.5	41.0	33.0	41.0
40-41	38.007183170478484	40.0	37.0	41.0	33.0	41.0
42-43	37.97226268467582	40.0	37.0	41.0	33.0	41.0
44-45	37.99937247960294	40.0	37.0	41.0	33.0	41.0
46-47	37.98085367190964	40.0	37.0	41.0	33.0	41.0
48-49	37.8959273032614	40.0	37.0	41.0	33.0	41.0
50-51	37.81167858472513	40.0	37.0	41.0	33.0	41.0
52-53	37.579582804715855	40.0	36.5	41.0	32.0	41.0
54-55	37.457585692516616	39.5	36.0	41.0	32.0	41.0
56-57	37.39067657047572	39.0	36.0	41.0	32.0	41.0
58-59	37.22425324283533	39.0	35.5	41.0	31.0	41.0
60-61	37.02793028177645	39.0	35.0	40.5	31.0	41.0
62-63	36.77517017745053	38.5	35.0	40.0	31.0	41.0
64-65	36.47737302991318	38.0	35.0	40.0	31.0	41.0
66-67	36.19156915879996	37.5	35.0	40.0	30.0	41.0
68-69	35.90357726403447	37.0	34.0	40.0	30.0	41.0
70-71	35.59645547290221	36.5	34.0	39.0	30.0	41.0
72-73	35.28461970553409	36.0	34.0	39.0	29.5	40.5
74-75	34.92958184249696	35.5	34.0	38.5	29.0	40.0
76-77	33.60052246892516	34.5	32.0	36.5	28.0	39.0
78-79	34.31554226072019	35.0	33.5	37.0	29.0	39.0
80-81	34.25835039093434	35.0	34.0	37.0	29.0	39.0
82-83	33.98370858031152	35.0	33.5	36.0	29.0	38.5
84-85	33.79741380406411	35.0	33.0	36.0	29.0	37.0
86-87	33.56380001792621	35.0	33.0	35.5	29.0	37.0
88-89	33.454027779986674	35.0	33.0	35.0	29.0	36.5
90-91	33.27450715918174	35.0	33.0	35.0	29.0	36.0
92-93	33.16913153257633	35.0	33.0	35.0	29.0	36.0
94-95	33.16727521551205	35.0	33.0	35.0	29.0	36.0
96-97	33.20500416936946	35.0	33.0	35.0	29.0	35.0
98-99	33.307215315875226	35.0	33.0	35.0	29.0	35.0
100	31.98008974180448	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	6.0
20	44.0
21	187.0
22	1025.0
23	3589.0
24	9940.0
25	21660.0
26	39425.0
27	63140.0
28	89554.0
29	119421.0
30	151002.0
31	186604.0
32	230371.0
33	290602.0
34	384312.0
35	534272.0
36	831831.0
37	1452381.0
38	1804168.0
39	750510.0
40	24730.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.301959213166825	20.371681732492462	14.6660323161069	23.66032673823381
2	14.531759857771927	21.341794520498944	41.37825298424974	22.748192637479388
3	17.976798509037707	26.40156536732117	30.79508211381823	24.826554009822896
4	12.556063115495919	18.33181065351224	39.18808374858255	29.92404248240929
5	13.066567459962583	38.05231961252151	35.789491005219084	13.091621922296826
6	29.661607363236048	38.29794205708439	18.93775375512876	13.102696824550797
7	26.270955925752364	32.91853007143598	23.32370122088635	17.486812781925302
8	24.440062815014077	36.590160650471645	21.27626086116666	17.69351567334762
9	25.563850603288845	17.039381007401154	21.284373870957356	36.112394518352644
10-11	24.055395974258722	26.838029554535662	29.1189228441322	19.987651627073415
12-13	24.926299673404852	24.377018862390045	29.170577103998912	21.52610436020619
14-15	22.01834370114934	25.837918662426535	26.82952305661579	25.314214579808336
16-17	21.5779918775937	28.979769821035912	27.464078581222452	21.97815972014794
18-19	21.61308012243924	28.164924527161244	29.14522543121843	21.07676991918109
20-21	22.476833962193414	27.360929723687434	29.030627291330376	21.131609022788776
22-23	22.124870267890724	27.249403074872813	28.919161940591998	21.706564716644465
24-25	21.916894843894855	27.389727182891505	28.936317529085574	21.757060444128065
26-27	21.866634052891456	27.57196339055996	28.819511897523814	21.741890659024772
28-29	21.83304786664299	27.698809922306882	28.828229376109093	21.639912834941036
30-31	22.114264636670303	27.451656701185666	28.769418280547033	21.664660381597
32-33	21.93378271583316	27.595349027506316	28.70276513077002	21.768103125890505
34-35	22.053521789257235	27.641030878453265	28.66335775579029	21.642089576499206
36-37	22.06116345881286	27.550879277784063	28.65269275285295	21.735264510550127
38-39	21.872080870057616	27.748002049861846	28.685847103673613	21.694069976406922
40-41	22.157828069693153	27.595992386882017	28.5494174118505	21.69676213157434
42-43	22.05807146103937	27.676494282247983	28.361491790817833	21.90394246589481
44-45	22.14001978018402	27.77688849525922	28.16798468596869	21.91510703858807
46-47	22.151945632890772	27.759949137192514	27.968431791791037	22.119673438125677
48-49	22.087886834564312	27.907964209078035	27.975133550789565	22.029015405568085
50-51	22.061421523984865	28.01251067394011	27.884792326882053	22.041275475192972
52-53	22.259983987314445	27.882281528409564	27.75821428830816	22.099520195967838
54-55	22.278450674484404	27.757270620726054	27.8143524756396	22.149926229149948
56-57	22.235448121335125	27.899250403014413	27.773322101399806	22.091979374250656
58-59	22.18280512497267	27.97232177851889	27.81929293085933	22.02558016564911
60-61	22.21827861172442	27.914384428434385	27.844003339336915	22.023333620504282
62-63	22.14645923608739	27.97368503389923	27.787893261365927	22.09196246864745
64-65	22.156241545310255	27.93251264158549	27.783806754720576	22.127439058383686
66-67	22.24583659512858	27.952400397244443	27.626855137534722	22.17490787009226
68-69	22.195628446045227	27.919400693442466	27.745511144454326	22.13945971605798
70-71	22.15161845712362	27.73707229083493	27.795080799538773	22.316228452502674
72-73	22.225765318798	27.76896773696539	27.736968566320286	22.268298377916327
74-75	22.07567027253024	27.92191086740963	27.724877895623784	22.277540964436348
76-77	22.1984675966082	27.82009701056552	27.642353004229665	22.33908238859662
78-79	22.21202666459248	27.85348598572781	27.614005192194853	22.32048215748486
80-81	22.177746202265887	27.857661342348113	27.64882741440222	22.31576504098378
82-83	22.244959205550067	27.78156006538245	27.74384579377359	22.22963493529389
84-85	22.303090055115447	27.783080868232357	27.68964856158978	22.224180515062418
86-87	22.12714202840575	27.79775879946739	27.767156583674378	22.30794258845248
88-89	22.209011487017612	27.76272312124422	27.724167367000803	22.304098024737364
90-91	22.254242457533437	27.745476182251817	27.79986960469063	22.20041175552412
92-93	22.238420159077737	27.68471358732495	27.81846737001617	22.258398883581144
94-95	22.074235634144948	27.811441478544424	27.85623734892121	22.258085538389416
96-97	22.129516654975507	27.923957222730543	27.955883019993482	21.990643102300464
98-99	22.248495351588627	28.724557971157555	27.071459905014176	21.95548677223964
100	20.46670930720145	28.541091865177236	28.120488619618893	22.871710208002415
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1068.0
1	943.0
2	1410.5
3	2180.0
4	2949.5
5	4649.5
6	6203.0
7	7460.5
8	8484.0
9	8713.0
10	8162.5
11	7347.0
12	7641.0
13	9851.0
14	15436.5
15	23403.5
16	30265.0
17	34751.5
18	36138.0
19	34751.5
20	31911.0
21	29017.5
22	27590.5
23	28983.5
24	33536.0
25	41462.5
26	53146.0
27	66079.5
28	80058.0
29	95948.0
30	111315.5
31	126604.5
32	143392.5
33	160954.5
34	178348.0
35	193645.0
36	209041.5
37	223618.5
38	234731.0
39	243116.0
40	253277.5
41	263416.5
42	269000.0
43	273290.5
44	276718.0
45	279896.0
46	281336.5
47	280369.0
48	278435.0
49	273858.5
50	266587.0
51	257395.0
52	245845.5
53	231371.5
54	216889.5
55	201875.0
56	184896.5
57	166476.5
58	147385.5
59	128215.0
60	107873.5
61	87862.0
62	68775.5
63	52764.5
64	40019.0
65	29880.0
66	22504.5
67	17435.0
68	13485.5
69	9789.0
70	7024.5
71	5088.5
72	3525.5
73	2408.0
74	1657.0
75	1118.0
76	728.0
77	448.0
78	295.5
79	189.5
80	121.5
81	91.0
82	77.0
83	57.5
84	42.0
85	30.5
86	23.0
87	20.0
88	12.5
89	7.5
90	4.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.001180464387535727
18-19	3.5771648107143243E-5
20-21	0.0
22-23	0.00737694085632474
24-25	0.008866973921506885
26-27	0.02153420161696941
28-29	0.017117191382589925
30-31	0.002121642661761276
32-33	0.0066677963880353176
34-35	0.03440303897105795
36-37	0.005967274687274808
38-39	0.0025738291318705693
40-41	0.007488894013717738
42-43	0.007317767188881191
44-45	0.008405944842491136
46-47	0.004983988200629118
48-49	9.195100227704792E-4
50-51	0.0025861998290275965
52-53	8.91000001497479E-4
54-55	0.0050098327370303435
56-57	8.392125464543837E-4
58-59	0.001451894368679461
60-61	7.41491802005483E-4
62-63	0.0013914201802973057
64-65	3.7114476277238644E-4
66-67	0.005408470240262146
68-69	0.004098080625152404
70-71	0.0017126028311455687
72-73	0.0015899956203570638
74-75	7.368658468446764E-4
76-77	7.910074399899783E-4
78-79	7.810687167984774E-4
80-81	9.110342249294256E-4
82-83	0.007148054006488411
84-85	7.608299969683851E-4
86-87	1.182529711481333E-4
88-89	4.5279098655467065E-4
90-91	3.289739380786208E-4
92-93	0.0023720878009849964
94-95	0.0032806914946776813
96-97	6.414478687986194E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	107.0
20-21	468.0
22-23	1041.0
24-25	2283.0
26-27	2633.0
28-29	4545.0
30-31	8655.0
32-33	11340.0
34-35	15047.0
36-37	20818.0
38-39	24899.0
40-41	28030.0
42-43	36369.0
44-45	40645.0
46-47	40120.0
48-49	35110.0
50-51	31356.0
52-53	30303.0
54-55	32749.0
56-57	35675.0
58-59	36434.0
60-61	36742.0
62-63	37213.0
64-65	40263.0
66-67	43803.0
68-69	45061.0
70-71	46312.0
72-73	46182.0
74-75	48536.0
76-77	47499.0
78-79	52783.0
80-81	55327.0
82-83	55805.0
84-85	59281.0
86-87	64828.0
88-89	74288.0
90-91	82652.0
92-93	93608.0
94-95	116692.0
96-97	285374.0
98-99	133535.0
100-101	5084364.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.28167019919283
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.46245453401946	76.2950754278053
2	7.541127342499916	12.862398699063867
3	1.7303051725999747	4.426899452208854
4	0.6145350120274786	2.096342888863377
5	0.25708579185073066	1.0962352856756177
6	0.1381371361551992	0.7068339412709217
7	0.08028829937108291	0.4792984187473127
8	0.04227324423510064	0.28841062984862265
9	0.029799983227746088	0.22872531074131083
>10	0.10191248237917432	1.300178587894352
>50	0.0015347906842905557	0.08068592991174703
>100	4.6788871785739214E-4	0.06597591578005843
>500	2.610753431706991E-5	0.011622299365607668
>1k	5.221469773800722E-5	0.0613172128229948
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.723463697142918E-5	0.0	0.0	0.0
3	0.0	5.723463697142918E-5	0.0	0.0	0.0
4	0.0	1.0016061470000107E-4	0.0	0.0	0.0
5	1.4308659242857296E-5	1.1446927394285837E-4	0.0	0.0	0.0
6	2.861731848571459E-5	1.1446927394285837E-4	0.0	0.0	0.0
7	2.861731848571459E-5	1.1446927394285837E-4	0.0	0.0	0.0
8	2.861731848571459E-5	1.1446927394285837E-4	0.0	0.0	1.4308659242857296E-5
9	2.861731848571459E-5	1.2877793318571566E-4	0.0	0.0	1.4308659242857296E-5
10-11	2.861731848571459E-5	1.2877793318571566E-4	0.0	1.4308659242857296E-5	1.4308659242857296E-5
12-13	2.861731848571459E-5	1.2877793318571566E-4	0.0	1.4308659242857296E-5	2.1462988864285943E-5
14-15	4.2925977728571885E-5	1.7885824053571618E-4	0.0	1.4308659242857296E-5	2.861731848571459E-5
16-17	7.869762583571513E-5	1.8601257015714485E-4	0.0	2.1462988864285943E-5	4.2925977728571885E-5
18-19	8.585195545714377E-5	2.074755590214308E-4	0.0	2.861731848571459E-5	5.723463697142919E-5
20-21	8.585195545714377E-5	2.1462988864285945E-4	0.0	6.438896659285783E-5	7.154329621428649E-5
22-23	8.585195545714377E-5	2.575558663714313E-4	0.0	2.3609287750714537E-4	7.869762583571513E-5
24-25	1.0731494432142971E-4	2.790188552357173E-4	0.0	5.365747216071485E-4	1.0016061470000107E-4
26-27	1.1446927394285837E-4	2.8617318485714595E-4	0.0	0.0013664769576928718	1.0016061470000107E-4
28-29	1.1446927394285837E-4	2.8617318485714595E-4	0.0	0.0043212150913429035	1.0016061470000107E-4
30-31	1.1446927394285837E-4	3.577164810714324E-4	0.0	0.004764783527871479	1.0016061470000107E-4
32-33	1.1446927394285837E-4	3.720251403142897E-4	0.0	0.005673383389792917	1.1446927394285837E-4
34-35	1.5024092205000162E-4	4.435684365285762E-4	0.0	0.006889619425435789	1.2877793318571566E-4
36-37	1.8601257015714485E-4	5.151117327428627E-4	0.0	0.008907140378678666	1.2877793318571566E-4
38-39	1.8601257015714485E-4	5.2942039198572E-4	0.0	0.01375777586200729	1.2877793318571566E-4
40-41	1.8601257015714485E-4	5.580377104714346E-4	0.0	0.0185797940268502	1.5739525167143026E-4
42-43	1.8601257015714485E-4	5.651920400928632E-4	0.0	0.02328734291775025	1.5739525167143026E-4
44-45	1.8601257015714485E-4	6.29581006685721E-4	0.0	0.02873178775965745	1.645495812928589E-4
46-47	1.8601257015714485E-4	6.939699732785788E-4	0.0	0.03479150494900751	1.7885824053571618E-4
48-49	1.8601257015714485E-4	7.082786325214362E-4	0.0	0.04078683317176472	1.8601257015714485E-4
50-51	1.8601257015714485E-4	8.012849176000085E-4	0.0	0.04664622913171478	1.8601257015714485E-4
52-53	1.8601257015714485E-4	8.155935768428659E-4	0.0	0.06048270261955779	1.931668997785735E-4
54-55	1.8601257015714485E-4	8.227479064642946E-4	0.0	0.07632954273102224	2.0032122940000214E-4
56-57	1.8601257015714485E-4	8.72828213814295E-4	0.0	0.09374318102957957	2.0032122940000214E-4
58-59	1.8601257015714485E-4	8.72828213814295E-4	0.0	0.11006220689605833	2.0032122940000214E-4
60-61	1.8601257015714485E-4	9.014455323000097E-4	0.0	0.11755994433931555	2.0032122940000214E-4
62-63	2.0032122940000214E-4	9.157541915428669E-4	0.0	0.12635261544405135	2.0032122940000214E-4
64-65	2.0032122940000214E-4	0.0010731494432142973	0.0	0.1307811454797157	2.0032122940000214E-4
66-67	2.0032122940000214E-4	0.0010874581024571544	0.0	0.13445131657550857	2.0032122940000214E-4
68-69	2.0032122940000214E-4	0.0011160754209428691	0.0	0.1383790435376729	2.1462988864285942E-4
70-71	2.0032122940000214E-4	0.0011446927394285836	0.0	0.1416199548561801	2.3609287750714537E-4
72-73	2.0032122940000214E-4	0.0011661557282928695	0.0	0.14438868041967298	2.7186452561428866E-4
74-75	2.0032122940000214E-4	0.0011876187171571556	0.0	0.14663513992080157	2.8617318485714595E-4
76-77	2.0032122940000214E-4	0.0012233903652642988	0.0	0.14869558685177303	2.8617318485714595E-4
78-79	2.1462988864285945E-4	0.001259162013371442	0.0	0.15015507009454446	2.8617318485714595E-4
80-81	2.1462988864285945E-4	0.001259162013371442	0.0	0.15060579286069448	2.9332751447857456E-4
82-83	2.1462988864285945E-4	0.001259162013371442	0.0	0.15099212666025164	3.290991625857178E-4
84-85	2.1462988864285945E-4	0.001259162013371442	0.0	0.1511352132526802	3.290991625857178E-4
86-87	2.1462988864285945E-4	0.0013163966503428712	0.0	0.1511852935600302	3.290991625857178E-4
88	2.1462988864285945E-4	0.0013879399465571577	0.0	0.15119960221927306	3.290991625857178E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8165	0.0	49.018578	1
GTATCAA	14570	0.0	37.598316	1
TCAACGC	17195	0.0	31.120008	4
ATCAACG	17335	0.0	30.944458	3
CAACGCA	17755	0.0	30.212461	5
AACGCAG	18185	0.0	29.61846	6
TATCAAC	18385	0.0	29.36771	2
ACGCAGA	21040	0.0	25.599415	7
CGCAGAG	21440	0.0	25.101389	8
TACATGG	20000	0.0	21.434994	2
GTACATG	20315	0.0	21.425957	1
GCAGAGT	25945	0.0	20.67536	9
ACATGGG	20420	0.0	20.32934	3
GTGGTAT	4055	0.0	20.301947	1
GAGTACT	14480	0.0	20.035511	12-13
AGAGTAC	22835	0.0	18.620928	10-11
CAGAGTA	24975	0.0	18.524527	10-11
CATGGGG	15335	0.0	18.361067	4
TGGTATC	4080	0.0	17.816345	2
GTACTTT	16400	0.0	17.704191	14-15
>>END_MODULE