FastQCFastQC Report
Fri 27 May 2016
SRR2139358_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139358_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13839255
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA157800.051.3250541
GTATCAA287900.037.8061941
TCAACGC338800.031.3029214
ATCAACG340000.031.2054633
CAACGCA347800.030.5310925
TATCAAC361650.029.7046952
AACGCAG361100.029.4065766
ACGCAGA418350.025.3294457
CGCAGAG426750.024.8204948
GTACATG384000.021.2643661
TACATGG384150.020.7142872
GCAGAGT514050.020.5449839
GTGGTAT79200.020.4634381
ACATGGG389950.019.8043383
GAGTACT299450.019.64578612-13
TGGTATC75350.019.2758432
CAGAGTA492950.018.65796310-11
AGAGTAC453150.018.57679210-11
GTACTTT332650.017.73202314-15
CATGGGG299950.017.4055484
Produced by FastQC (version 0.11.5)