##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139358_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13839255
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.060323695170005	33.0	31.0	34.0	30.0	34.0
2	32.29936351342612	34.0	31.0	34.0	30.0	34.0
3	32.341428133234054	34.0	31.0	34.0	30.0	34.0
4	36.00592828154406	37.0	35.0	37.0	35.0	37.0
5	35.84931826171279	37.0	35.0	37.0	35.0	37.0
6	35.87501487616205	37.0	35.0	37.0	35.0	37.0
7	35.82675129549965	37.0	35.0	37.0	35.0	37.0
8	35.825413145433046	37.0	35.0	37.0	35.0	37.0
9	37.5864690693249	39.0	37.0	39.0	35.0	39.0
10-11	37.45978233654918	39.0	37.0	39.0	34.5	39.0
12-13	37.41944226766542	39.0	37.0	39.0	34.0	39.0
14-15	38.65593389962104	40.0	38.0	41.0	34.0	41.0
16-17	38.6332414931295	40.0	38.0	41.0	34.0	41.0
18-19	38.60519970186257	40.0	38.0	41.0	34.0	41.0
20-21	38.590410078665855	40.0	38.0	41.0	34.0	41.0
22-23	38.5074192939543	40.0	38.0	41.0	34.0	41.0
24-25	38.434659290228105	40.0	38.0	41.0	34.0	41.0
26-27	38.24678316561979	40.0	38.0	41.0	34.0	41.0
28-29	38.10064516261438	40.0	38.0	41.0	33.0	41.0
30-31	37.95578617295897	40.0	37.0	41.0	33.0	41.0
32-33	37.86544900514734	40.0	37.0	41.0	33.0	41.0
34-35	37.933161693639704	40.0	37.0	41.0	33.0	41.0
36-37	37.89126307352545	40.0	37.0	41.0	33.0	41.0
38-39	37.81941222630349	40.0	37.0	41.0	33.0	41.0
40-41	37.73850690043551	40.0	37.0	41.0	33.0	41.0
42-43	37.70103657317684	40.0	37.0	41.0	32.5	41.0
44-45	37.72697779355806	40.0	37.0	41.0	33.0	41.0
46-47	37.7040237176523	40.0	37.0	41.0	32.0	41.0
48-49	37.61292065916647	40.0	37.0	41.0	32.0	41.0
50-51	37.52004353059348	40.0	36.5	41.0	32.0	41.0
52-53	37.26773530875016	39.0	36.0	41.0	31.0	41.0
54-55	37.13629764494367	39.0	36.0	41.0	31.0	41.0
56-57	37.067124335848135	39.0	35.0	41.0	31.0	41.0
58-59	36.89717089672671	39.0	35.0	40.0	31.0	41.0
60-61	36.693982987940245	38.5	35.0	40.0	31.0	41.0
62-63	36.43236576999332	38.0	35.0	40.0	30.0	41.0
64-65	36.12343669370221	38.0	34.5	40.0	30.0	41.0
66-67	35.83079150355138	37.0	34.0	40.0	29.0	41.0
68-69	35.54056327603087	37.0	34.0	39.0	29.0	41.0
70-71	35.23626146977051	36.0	34.0	39.0	29.0	40.5
72-73	34.922634265014835	36.0	34.0	39.0	29.0	40.0
74-75	34.570631951751295	35.0	33.0	38.0	29.0	40.0
76-77	33.272688279461306	34.5	31.5	36.5	27.0	39.0
78-79	34.02756422894558	35.0	33.0	37.0	29.0	39.0
80-81	33.990147165056584	35.0	33.0	36.5	29.0	39.0
82-83	33.71686656476078	35.0	33.0	36.0	29.0	37.5
84-85	33.53982659384464	35.0	33.0	36.0	29.0	37.0
86-87	33.30592187286658	35.0	33.0	35.0	29.0	37.0
88-89	33.198694789461484	35.0	33.0	35.0	29.0	36.0
90-91	33.017585275581744	35.0	33.0	35.0	29.0	36.0
92-93	32.915035775087745	34.5	32.0	35.0	29.0	36.0
94-95	32.92169020071104	34.0	32.0	35.0	29.0	35.5
96-97	32.97639692395494	34.0	32.0	35.0	29.0	35.0
98-99	33.09979124860169	34.0	32.5	35.0	29.0	35.0
100	31.69848246797046	34.0	30.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	55.0
21	394.0
22	2294.0
23	8140.0
24	22939.0
25	49907.0
26	90410.0
27	143834.0
28	205765.0
29	271582.0
30	341635.0
31	419721.0
32	514288.0
33	643880.0
34	849200.0
35	1166734.0
36	1791031.0
37	2948551.0
38	3257640.0
39	1085720.0
40	25530.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.04139276283297	20.586122591136586	14.766112771243828	23.606371874786614
2	14.740280455848238	21.35723346379556	41.07771697248154	22.824769107874666
3	18.21139215947679	26.132815675410274	30.73908241447968	24.916709750633252
4	12.714022539508088	18.276019915811943	38.84790041082414	30.162057133855836
5	13.152738351883825	37.99192225304036	35.705274597512656	13.150064797563163
6	30.002807232036695	38.13310037281631	18.696309880842573	13.167782514304418
7	26.49140434221351	32.771193247035335	23.11323839325166	17.624164017499496
8	24.53316309295551	36.7138910295388	21.067752563270208	17.685193314235484
9	25.431766377597636	17.006428452976696	21.285235368522365	36.2765698009033
10-11	24.15941465057187	26.834453877755703	28.9315031770135	20.074628294658925
12-13	24.962662368747452	24.32573140678454	29.069379095912318	21.642227128555692
14-15	22.025860496103295	25.80695637156769	26.6741670704095	25.493016061919516
16-17	21.654502332956525	28.956090471831537	27.380864293697883	22.00854290151406
18-19	21.66253981656156	28.141154351058468	29.078618617088825	21.117687215291152
20-21	22.442889788670474	27.337678101276264	28.96273702532024	21.256695084733018
22-23	22.155161710104608	27.23180801975966	28.830284647885758	21.782745622249973
24-25	21.97545158264416	27.362933449021305	28.880766972776424	21.780847995558116
26-27	21.939539447629908	27.543291593008547	28.752058594402012	21.765110364959533
28-29	21.84754199945666	27.65452374329009	28.77252448197264	21.725409775280614
30-31	22.129820707617743	27.45736578045691	28.702399185473897	21.71041432645145
32-33	21.97622983230403	27.58576343875305	28.640581480158723	21.7974252487842
34-35	22.091320533969316	27.615364841258298	28.6240401765572	21.669274448215177
36-37	22.094763454952247	27.531704560886443	28.577915529767356	21.795616454393954
38-39	21.943101066136563	27.703412247112702	28.60698032380085	21.746506362949884
40-41	22.19138881694103	27.557534468995904	28.505831651361696	21.745245062701375
42-43	22.0862442880311	27.65203527872956	28.315592272450868	21.946128160788476
44-45	22.18412610212884	27.784455960076578	28.116137854239415	21.91528008355517
46-47	22.23572062533904	27.74739372025456	27.902957971956077	22.113927682450317
48-49	22.15371278659285	27.870674691206325	27.914792722804894	22.06081979939593
50-51	22.168931378160607	27.95324731137982	27.814930122707487	22.062891187752083
52-53	22.30065055347602	27.889374353188636	27.71291997719281	22.09705511614253
54-55	22.298439712342695	27.782695035204906	27.73707437074777	22.18179088170463
56-57	22.260488759775665	27.906809823916916	27.723768533926446	22.108932882380973
58-59	22.234079160264905	27.939797743031463	27.76708479624088	22.059038300462745
60-61	22.277139704191974	27.915191224501772	27.771045369139703	22.03662370216655
62-63	22.218555132463162	27.991872831447473	27.701111885586187	22.088460150503177
64-65	22.200782669435057	27.95714053749194	27.678115905407108	22.163960887665894
66-67	22.257651920376485	27.977473630404724	27.582833302199106	22.18204114701968
68-69	22.19632755986791	27.975960249027537	27.65562113381063	22.17209105729392
70-71	22.200650813348634	27.794770755427866	27.721179752492702	22.283398678730798
72-73	22.248217885848437	27.84861049988534	27.678231862165763	22.224939752100457
74-75	22.104415253946556	28.00786016998027	27.61679055826778	22.270934017805395
76-77	22.21096661689603	27.908562176102343	27.587788709562616	22.29268249743901
78-79	22.202006151093094	27.952741633157164	27.53128661018821	22.313965605561535
80-81	22.170784239516667	27.955900726695624	27.579310477743068	22.294004556044637
82-83	22.209809535572074	27.930919780216556	27.67471242305673	22.184558261154635
84-85	22.28523350943312	27.94572518862274	27.613075981096426	22.15596532084772
86-87	22.122196201855843	27.961941844999476	27.68297863738287	22.23288331576181
88-89	22.183541626163866	27.957587276820057	27.60173398851934	22.25713710849674
90-91	22.17993302916063	28.001124002732947	27.66084244446152	22.1581005236449
92-93	22.167445357207246	27.97021093263955	27.693645640673214	22.168698069479987
94-95	21.983439857440587	28.091754660842234	27.747653435106745	22.177152046610438
96-97	22.009300848653528	28.2310509095415	27.79428118571765	21.965367056087324
98-99	22.165619918222333	29.053674947283408	26.899524946359175	21.881180188135083
100	20.441530583437952	28.90105471378739	27.907326457235765	22.75008824553889
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3372.0
1	2726.0
2	3118.5
3	4402.5
4	5706.0
5	8871.0
6	11785.5
7	14353.5
8	16634.0
9	17108.0
10	15792.5
11	14035.5
12	14683.5
13	19060.5
14	29973.0
15	45887.0
16	59418.5
17	67619.5
18	70419.0
19	68042.5
20	62763.0
21	57054.5
22	53775.0
23	56349.0
24	64820.0
25	79307.0
26	100482.5
27	125250.0
28	152562.0
29	182377.5
30	211465.5
31	243072.0
32	277521.0
33	309214.5
34	340029.5
35	369758.0
36	402820.0
37	433731.5
38	453242.5
39	468079.0
40	487098.0
41	506304.5
42	518383.0
43	528158.5
44	534729.0
45	542782.5
46	549450.5
47	549857.5
48	547433.5
49	537968.5
50	525523.5
51	508650.5
52	484597.0
53	457917.0
54	430102.5
55	399381.0
56	366860.5
57	331653.5
58	292667.5
59	253114.0
60	211682.5
61	171888.5
62	136273.0
63	104385.5
64	77760.0
65	57211.5
66	42707.5
67	32427.5
68	24627.5
69	17922.5
70	12535.0
71	8880.5
72	6111.5
73	4021.5
74	2617.5
75	1723.5
76	1126.5
77	731.5
78	488.0
79	344.0
80	247.5
81	172.0
82	131.0
83	88.5
84	55.0
85	38.0
86	32.0
87	24.5
88	17.0
89	16.0
90	14.0
91	11.5
92	9.5
93	4.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.001177809065589152
18-19	5.4193668662077545E-5
20-21	0.0
22-23	0.006485964679568232
24-25	0.008297848526172357
26-27	0.020411824281307476
28-29	0.01622161511225195
30-31	0.001867612952851351
32-33	0.006167044286407393
34-35	0.03243278235099281
36-37	0.0054462759597488945
38-39	0.002777126926618138
40-41	0.007258835603821087
42-43	0.007427653553991009
44-45	0.008102087332027224
46-47	0.00522778151205435
48-49	0.00101915668652522
50-51	0.002498296005280703
52-53	9.144377807914249E-4
54-55	0.005299374211229144
56-57	9.552384331763898E-4
58-59	0.0012397689207220095
60-61	8.149145819101681E-4
62-63	0.0013655770165355312
64-65	3.458875792346776E-4
66-67	0.005501472551805115
68-69	0.003842414238984801
70-71	0.0017195690119125879
72-73	0.001584618115881196
74-75	6.805843798208472E-4
76-77	6.054171692724498E-4
78-79	7.980140278033701E-4
80-81	9.454464852845093E-4
82-83	0.006639271592886709
84-85	6.736737776630889E-4
86-87	4.1466702134519226E-5
88-89	3.897473481674247E-4
90-91	2.8006634177556546E-4
92-93	0.00221695023325545
94-95	0.0033469289911339673
96-97	4.497724488738035E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	281.0
20-21	965.0
22-23	2104.0
24-25	4388.0
26-27	5588.0
28-29	8232.0
30-31	14222.0
32-33	18647.0
34-35	25000.0
36-37	31490.0
38-39	37947.0
40-41	44104.0
42-43	59850.0
44-45	65356.0
46-47	64307.0
48-49	55171.0
50-51	48542.0
52-53	46769.0
54-55	50263.0
56-57	54009.0
58-59	56900.0
60-61	58861.0
62-63	60348.0
64-65	66203.0
66-67	71701.0
68-69	74611.0
70-71	77817.0
72-73	78997.0
74-75	83795.0
76-77	81594.0
78-79	91962.0
80-81	98643.0
82-83	102045.0
84-85	110579.0
86-87	122831.0
88-89	142708.0
90-91	161809.0
92-93	189473.0
94-95	253551.0
96-97	606009.0
98-99	310779.0
100-101	1.0300804E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.15975508043745
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.79563439889033	69.5751679546624
2	8.573006917701285	13.744202696516616
3	2.453470568740386	5.900087996618727
4	0.9814883170454554	3.1470345243469775
5	0.45512332298146424	1.82412870507945
6	0.23812938495338487	1.145303590519112
7	0.15379248559312195	0.8629577584849452
8	0.08520307837330958	0.546388631560305
9	0.06057751583678115	0.43702909495719267
>10	0.19926779746621434	2.4042125751640624
>50	0.0033148972235928667	0.1769169898037189
>100	8.689006841810269E-4	0.12009903804781803
>500	6.995657892654642E-5	0.038733734599495306
>1k	5.2457931565045836E-5	0.07773670963917838
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	7.225822488277007E-6	0.0
3	0.0	7.225822488277007E-6	0.0	1.4451644976554013E-5	0.0
4	0.0	2.1677467464831017E-5	0.0	1.4451644976554013E-5	0.0
5	0.0	2.1677467464831017E-5	0.0	2.1677467464831017E-5	0.0
6	5.0580757417939044E-5	2.1677467464831017E-5	0.0	2.1677467464831017E-5	1.4451644976554013E-5
7	5.0580757417939044E-5	2.1677467464831017E-5	0.0	2.1677467464831017E-5	1.4451644976554013E-5
8	5.0580757417939044E-5	2.1677467464831017E-5	0.0	2.8903289953108027E-5	2.1677467464831017E-5
9	5.0580757417939044E-5	5.780657990621605E-5	0.0	5.0580757417939044E-5	3.612911244138503E-5
10-11	5.780657990621605E-5	5.780657990621605E-5	0.0	6.141949115035456E-5	3.612911244138503E-5
12-13	5.780657990621605E-5	5.780657990621605E-5	0.0	7.225822488277006E-5	3.612911244138503E-5
14-15	5.780657990621605E-5	9.393569234760109E-5	0.0	7.948404737104707E-5	5.0580757417939044E-5
16-17	9.393569234760109E-5	9.393569234760109E-5	0.0	8.309695861518557E-5	7.225822488277006E-5
18-19	9.393569234760109E-5	1.0838733732415509E-4	0.0	8.670986985932407E-5	7.225822488277006E-5
20-21	9.393569234760109E-5	1.2645189354484762E-4	0.0	9.393569234760109E-5	7.225822488277006E-5
22-23	9.393569234760109E-5	1.7341973971864814E-4	0.0	1.0477442608001659E-4	1.0116151483587809E-4
24-25	9.393569234760109E-5	2.0232302967175618E-4	0.0	1.156131598124321E-4	1.156131598124321E-4
26-27	9.393569234760109E-5	2.0954885216003318E-4	0.0	1.192260710565706E-4	1.2645189354484762E-4
28-29	9.393569234760109E-5	2.0954885216003318E-4	0.0	1.7703265096278664E-4	1.3367771603312463E-4
30-31	9.754860359173959E-5	2.2400049713658718E-4	0.0	4.299364380524819E-4	1.4090353852140163E-4
32-33	1.0477442608001659E-4	2.2400049713658718E-4	0.0	0.0010874862844856895	1.5896809474209413E-4
34-35	1.156131598124321E-4	2.745812545545262E-4	0.0	0.002012391562985146	1.6619391723037114E-4
36-37	1.156131598124321E-4	3.2877492321660374E-4	0.0	0.003302200877142592	1.6619391723037114E-4
38-39	1.156131598124321E-4	3.323878344607423E-4	0.0	0.00683201516266591	1.6980682847450964E-4
40-41	1.192260710565706E-4	3.540653019255733E-4	0.0	0.010148667684785055	1.7341973971864814E-4
42-43	1.3006480478898612E-4	3.685169469021273E-4	0.0	0.012970351366457227	1.7341973971864814E-4
44-45	1.3006480478898612E-4	4.3354934929662036E-4	0.0	0.01668081121418747	1.7703265096278664E-4
46-47	1.3006480478898612E-4	4.8413010671455943E-4	0.0	0.020597207002833608	1.9148429593934067E-4
48-49	1.3006480478898612E-4	4.8413010671455943E-4	0.0	0.024585861016362516	2.0954885216003318E-4
50-51	1.3729062727726313E-4	5.347108641324985E-4	0.0	0.02843361149137002	2.0954885216003318E-4
52-53	1.3729062727726313E-4	5.419366866207755E-4	0.0	0.03813789109312604	2.203875858924487E-4
54-55	1.3729062727726313E-4	5.419366866207755E-4	0.0	0.050060498198783096	2.348392308690027E-4
56-57	1.3729062727726313E-4	5.491625091090525E-4	0.0	0.06333072119850382	2.3845214211314121E-4
58-59	1.3729062727726313E-4	5.52775420353191E-4	0.0	0.07572661967714303	2.456779646014182E-4
60-61	1.5174227225381713E-4	5.852916215504376E-4	0.0	0.08105566376224732	2.456779646014182E-4
62-63	2.2400049713658718E-4	6.141949115035456E-4	0.0	0.08776483994261253	2.7458125455452625E-4
64-65	2.2400049713658718E-4	7.081306038511466E-4	0.0	0.09117542815707927	2.7458125455452625E-4
66-67	2.348392308690027E-4	7.153564263394236E-4	0.0	0.09368640147175553	2.7458125455452625E-4
68-69	2.3845214211314121E-4	7.334209825601162E-4	0.0	0.09655144008835735	2.7458125455452625E-4
70-71	2.3845214211314121E-4	7.442597162925316E-4	0.0	0.09911299416045155	2.7458125455452625E-4
72-73	2.7458125455452625E-4	7.478726275366701E-4	0.0	0.10102422420860083	2.8180707704280326E-4
74-75	2.7458125455452625E-4	7.803888287339167E-4	0.0	0.10255971148735968	2.8180707704280326E-4
76-77	2.7458125455452625E-4	8.092921186870247E-4	0.0	0.10415300534602477	2.8180707704280326E-4
78-79	2.7458125455452625E-4	8.309695861518558E-4	0.0	0.10526939492046355	2.8541998828694173E-4
80-81	2.7458125455452625E-4	8.309695861518558E-4	0.0	0.10569210553602777	3.070974557517728E-4
82-83	2.781941657986648E-4	8.309695861518558E-4	0.0	0.1059775255243147	3.396136569490193E-4
84-85	2.8180707704280326E-4	8.309695861518558E-4	0.0	0.10611842906283611	3.396136569490193E-4
86-87	2.8180707704280326E-4	8.454212311284098E-4	0.0	0.10619791311020715	3.576782131697118E-4
88	2.8180707704280326E-4	9.176794560111798E-4	0.0	0.10620513893269544	3.7574276939040434E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15780	0.0	51.325054	1
GTATCAA	28790	0.0	37.806194	1
TCAACGC	33880	0.0	31.302921	4
ATCAACG	34000	0.0	31.205463	3
CAACGCA	34780	0.0	30.531092	5
TATCAAC	36165	0.0	29.704695	2
AACGCAG	36110	0.0	29.406576	6
ACGCAGA	41835	0.0	25.329445	7
CGCAGAG	42675	0.0	24.820494	8
GTACATG	38400	0.0	21.264366	1
TACATGG	38415	0.0	20.714287	2
GCAGAGT	51405	0.0	20.544983	9
GTGGTAT	7920	0.0	20.463438	1
ACATGGG	38995	0.0	19.804338	3
GAGTACT	29945	0.0	19.645786	12-13
TGGTATC	7535	0.0	19.275843	2
CAGAGTA	49295	0.0	18.657963	10-11
AGAGTAC	45315	0.0	18.576792	10-11
GTACTTT	33265	0.0	17.732023	14-15
CATGGGG	29995	0.0	17.405548	4
>>END_MODULE