FastQCFastQC Report
Fri 27 May 2016
SRR2139357_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139357_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8595001
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107550.046.2723161
GTATCAA194200.035.498791
ATCAACG227600.029.446833
TCAACGC228350.029.3664864
CAACGCA233150.028.6713565
TATCAAC241150.028.103512
AACGCAG239800.027.9293696
GTACATG269400.024.7851871
TACATGG265550.024.626572
ACGCAGA275000.024.293797
CGCAGAG279500.023.9181238
ACATGGG266950.023.7790623
CATGGGG196450.021.1726634
GCAGAGT328750.020.3488149
GAGTACT187900.018.96527112-13
AGAGTAC289500.018.1190110-11
CAGAGTA319450.017.95334610-11
GTGGTAT52100.017.2799841
GTACTTT213150.016.84314714-15
ATGGGGG118450.016.6419945
Produced by FastQC (version 0.11.5)