##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139357_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8595001
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60286962153931	33.0	33.0	33.0	33.0	33.0
2	32.614850888324504	33.0	33.0	33.0	33.0	33.0
3	32.666836455283715	33.0	33.0	33.0	33.0	33.0
4	36.59090382886517	37.0	37.0	37.0	37.0	37.0
5	36.59497607970028	37.0	37.0	37.0	37.0	37.0
6	36.17796810029458	37.0	37.0	37.0	33.0	37.0
7	36.049365090242574	37.0	37.0	37.0	33.0	37.0
8	35.95792484491857	37.0	37.0	37.0	33.0	37.0
9	35.967613267293395	37.0	37.0	37.0	33.0	37.0
10-11	35.88843858191523	37.0	37.0	37.0	33.0	37.0
12-13	35.8040538913259	37.0	37.0	37.0	33.0	37.0
14-15	37.524382545156186	40.0	37.0	40.0	33.0	40.0
16-17	37.46049215119346	40.0	37.0	40.0	33.0	40.0
18-19	37.491173124936225	40.0	37.0	40.0	33.0	40.0
20-21	37.408145302925995	40.0	37.0	40.0	33.0	40.0
22-23	37.29199396612722	40.0	37.0	40.0	33.0	40.0
24-25	37.27662911474045	40.0	37.0	40.0	33.0	40.0
26-27	37.12820312025178	40.0	37.0	40.0	33.0	40.0
28-29	36.971600333484915	40.0	37.0	40.0	33.0	40.0
30-31	36.78695840701267	40.0	37.0	40.0	33.0	40.0
32-33	36.57272622437073	40.0	37.0	40.0	33.0	40.0
34-35	36.48303482772512	40.0	37.0	40.0	33.0	40.0
36-37	36.306312671541505	40.0	37.0	40.0	33.0	40.0
38-39	36.33537944060535	40.0	37.0	40.0	33.0	40.0
40-41	36.361450757522995	40.0	37.0	40.0	33.0	40.0
42-43	36.27572988143696	37.0	37.0	40.0	33.0	40.0
44-45	36.152643903437145	37.0	37.0	40.0	27.0	40.0
46-47	35.987418264325534	37.0	37.0	40.0	27.0	40.0
48-49	35.79043978779628	37.0	37.0	40.0	27.0	40.0
50-51	35.778379961561875	37.0	37.0	40.0	27.0	40.0
52-53	35.66280099660088	37.0	37.0	40.0	27.0	40.0
54-55	35.47252673706191	37.0	33.0	40.0	27.0	40.0
56-57	35.233582150557744	37.0	33.0	40.0	27.0	40.0
58-59	35.109889270535966	37.0	33.0	40.0	27.0	40.0
60-61	34.98547166473479	37.0	33.0	40.0	27.0	40.0
62-63	34.814030763788224	37.0	33.0	40.0	27.0	40.0
64-65	34.63102549306488	37.0	33.0	40.0	27.0	40.0
66-67	34.3631421582777	37.0	33.0	38.5	27.0	40.0
68-69	34.149407900729756	37.0	33.0	37.0	27.0	40.0
70-71	33.913417105256585	37.0	33.0	37.0	27.0	40.0
72-73	33.61861304442398	37.0	33.0	37.0	27.0	40.0
74-75	33.26810002242719	37.0	33.0	37.0	24.5	38.5
76-77	31.167730671824586	33.0	30.0	35.0	22.0	37.0
78-79	33.25465516712555	35.0	33.0	37.0	27.0	37.0
80-81	33.60676466600014	37.0	33.0	37.0	27.0	37.0
82-83	33.55066426449862	37.0	33.0	37.0	27.0	37.0
84-85	33.449620082732494	37.0	33.0	37.0	27.0	37.0
86-87	33.31163924685645	37.0	33.0	37.0	27.0	37.0
88-89	33.14662119558864	35.0	33.0	37.0	27.0	37.0
90-91	33.06163883476863	33.0	33.0	37.0	27.0	37.0
92-93	33.10449254530272	33.0	33.0	37.0	27.0	37.0
94-95	33.15454210993522	33.0	33.0	37.0	27.0	37.0
96-97	33.264170496771484	33.0	33.0	37.0	27.0	37.0
98-99	33.41390732681409	33.0	33.0	37.0	27.0	37.0
100	33.38549969870396	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	13.0
16	28.0
17	108.0
18	330.0
19	957.0
20	2409.0
21	6478.0
22	16293.0
23	36201.0
24	68345.0
25	111639.0
26	160852.0
27	211083.0
28	254868.0
29	286570.0
30	309862.0
31	330064.0
32	357407.0
33	401991.0
34	482471.0
35	642087.0
36	1032453.0
37	2104780.0
38	1731734.0
39	45972.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.65516664861354	20.03331936785115	13.31660112663163	23.99491285690368
2	14.889525565810255	21.59204651555124	41.0603288549414	22.4580990636971
3	18.00229816423776	27.021126775312094	30.49811243831363	24.478462622136515
4	12.382913739691958	18.028442194976513	39.27951481303722	30.309129252294316
5	13.004911344375955	38.405705457324665	35.68105127179189	12.908331926507497
6	29.742125684453093	38.218110736694506	18.985687145353445	13.05407643349896
7	26.375843353595883	32.87121199869552	23.657600505223908	17.095344142484684
8	24.781218757275305	36.363055687835285	21.52650127673051	17.329224278158897
9	26.392387854288792	16.12573401678487	21.047234316784838	36.4346438121415
10-11	24.302704249801337	26.59188666886174	29.315083554489302	19.790325526847617
12-13	25.303151552157356	24.29485893688286	29.141223212528022	21.26076629843176
14-15	22.442985278153653	25.478084775402543	26.76054805804966	25.318381888394143
16-17	21.964616409003327	28.466168881190356	27.38807709271936	22.181137617086954
18-19	22.014540777831208	27.709647735933945	29.0340745742787	21.241736911956146
20-21	22.902198482127233	26.997954639118614	28.73471006458051	21.365136814173642
22-23	22.56773653844619	26.970503052745	28.573193450098405	21.888566958710403
24-25	22.312483492970053	27.078357898009898	28.703649761543577	21.905508847476472
26-27	22.241456767933744	27.26952009866878	28.632783133009514	21.856240000387963
28-29	22.216911125513555	27.365314492686387	28.570339852392124	21.847434529407934
30-31	22.50387322443547	27.219396532094216	28.4440317967266	21.832698446743713
32-33	22.287341202815576	27.418039791878773	28.389486879418946	21.90513212588671
34-35	22.413579471088678	27.43255580454051	28.366808101228276	21.78705662314254
36-37	22.455888430704942	27.375871542135283	28.3015948629708	21.866645164188974
38-39	22.225888164768904	27.55806670626921	28.379316231292805	21.83672889766908
40-41	22.436873526381888	27.430285250760118	28.239904577875002	21.89293664498299
42-43	22.379115056872276	27.451746063792942	28.03568352212176	22.13345535721302
44-45	22.381791310395617	27.59697366857392	27.853346366571014	22.167888654459453
46-47	22.473730658958242	27.518264288527632	27.62967202857853	22.3783330239356
48-49	22.38263296613643	27.646841218858725	27.6571692505104	22.313356564494445
50-51	22.35416265278138	27.7374365208782	27.54427137564936	22.36412945069106
52-53	22.483375694512617	27.678736132594228	27.422892070473377	22.414996102419778
54-55	22.466167954263497	27.54345382227525	27.46339785713766	22.526980366323592
56-57	22.430083936063898	27.657330303841604	27.435840873000465	22.47674488709404
58-59	22.37335760467176	27.68970960186447	27.489696829739184	22.447235963724584
60-61	22.43220585877658	27.711925186779744	27.497109109168033	22.358759845275642
62-63	22.352732728652313	27.823622520778112	27.4364426914332	22.387202059136374
64-65	22.340941594726708	27.8130143623753	27.39399568775736	22.45204835514063
66-67	22.4342229630451	27.869996632881854	27.236064893892664	22.459715510180377
68-69	22.300969725541698	27.846156619565658	27.350224889030432	22.502648765862208
70-71	22.329402903766827	27.570274539991207	27.408055981480018	22.69226657476195
72-73	22.390308035467193	27.623837053611883	27.286636043114427	22.699218867806497
74-75	22.280018891331448	27.725959871000327	27.243970388099903	22.75005084956832
76-77	22.406165113005937	27.605552269880235	27.303192924147947	22.68508969296588
78-79	22.38839443690631	27.642685735450467	27.232721564757195	22.736198262886028
80-81	22.4171536812547	27.635820007072947	27.226478871130006	22.720547440542347
82-83	22.410714884941456	27.602424435855582	27.323202683344604	22.663657995858355
84-85	22.504749908007536	27.627834612726897	27.249147475763174	22.618268003502394
86-87	22.31808308670041	27.632866291773787	27.384104318009893	22.664946303515915
88-89	22.382690827436765	27.583083013375393	27.35266112735119	22.68156503183665
90-91	22.452753992964467	27.543654632714965	27.42266540222218	22.58092597209838
92-93	22.463894726359985	27.544716709527123	27.39740795779798	22.593980606314908
94-95	22.34141318962598	27.584868118982257	27.499606531087302	22.57411216030446
96-97	22.37154308225525	27.591612151753882	27.68985893794318	22.34698582804769
98-99	23.443850527923857	29.492765211405292	27.93295178431662	19.13043247635423
100	22.54256392003322	30.343539931872748	21.635095628969797	25.478800519124235
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1319.0
1	1270.5
2	1947.5
3	2894.5
4	4162.5
5	7506.5
6	10471.0
7	11702.5
8	11682.0
9	10447.5
10	8975.5
11	7831.5
12	8397.0
13	11671.0
14	19179.5
15	30102.0
16	40165.0
17	47033.5
18	50038.5
19	48511.0
20	44729.5
21	40712.5
22	38026.5
23	38646.0
24	43115.0
25	52349.0
26	64973.0
27	79354.0
28	95067.0
29	113248.0
30	131851.0
31	151216.5
32	173459.5
33	194792.0
34	216301.0
35	237137.0
36	258577.0
37	279324.0
38	295058.0
39	308359.0
40	322926.5
41	336564.0
42	346264.0
43	354946.5
44	362587.5
45	370053.0
46	374046.5
47	375957.0
48	376617.0
49	371127.0
50	363278.0
51	353536.0
52	338976.5
53	320129.0
54	299607.5
55	278593.5
56	257004.0
57	233036.0
58	208356.0
59	184228.0
60	157611.5
61	130825.5
62	106767.5
63	85659.0
64	66991.5
65	51880.5
66	40288.5
67	31626.0
68	25203.5
69	19240.5
70	14308.5
71	10831.0
72	8110.5
73	5989.0
74	4354.5
75	3196.5
76	2390.0
77	1734.5
78	1258.0
79	904.5
80	645.5
81	465.5
82	357.5
83	264.0
84	192.0
85	137.0
86	94.5
87	75.5
88	62.0
89	44.5
90	34.0
91	30.5
92	23.0
93	14.5
94	7.5
95	4.0
96	4.0
97	3.5
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	3.025014191388692E-4
3	0.0036649210395670695
4	0.004374635907546724
5	2.326933993375917E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.2798136963567544E-4
12-13	0.0014543337458599482
14-15	0.003385688960361959
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0068516427498657075
26-27	0.0210881127359992
28-29	0.009588343760339903
30-31	0.0
32-33	0.0
34-35	1.7176338988909474E-4
36-37	0.01266779774412048
38-39	0.03577896441953532
40-41	0.040710177237946926
42-43	0.007259249059999778
44-45	3.1657614618220127E-4
46-47	0.0
48-49	0.03297817817062356
50-51	0.05701944005950505
52-53	0.05320814144828217
54-55	0.02846172445280295
56-57	0.0012773160422209153
58-59	0.005772925367040337
60-61	0.028415706223391197
62-63	0.0323936990935024
64-65	0.01587936943549074
66-67	0.04220926015140114
68-69	0.05917467872435198
70-71	0.0729136853155348
72-73	0.051278066646240295
74-75	0.023534288077522583
76-77	0.004742610572138653
78-79	4.925862509470514E-4
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0025426445625019688
92-93	0.0027346379634411323
94-95	6.079678554550429E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2304.0
20-21	6874.0
22-23	7923.0
24-25	14193.0
26-27	19232.0
28-29	27630.0
30-31	31218.0
32-33	33867.0
34-35	38714.0
36-37	38524.0
38-39	39441.0
40-41	49280.0
42-43	56499.0
44-45	66140.0
46-47	66686.0
48-49	53906.0
50-51	62465.0
52-53	59290.0
54-55	71494.0
56-57	76397.0
58-59	75532.0
60-61	74816.0
62-63	78933.0
64-65	92415.0
66-67	99332.0
68-69	91783.0
70-71	91458.0
72-73	82915.0
74-75	88024.0
76-77	71857.0
78-79	90397.0
80-81	102281.0
82-83	115541.0
84-85	123057.0
86-87	133401.0
88-89	146701.0
90-91	157935.0
92-93	170416.0
94-95	193801.0
96-97	356523.0
98-99	531561.0
100-101	4804245.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.8313511064303
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.14148253678361	79.13938070512043
2	6.096262955796878	10.586934983038464
3	1.51477502807275	3.9458988692951333
4	0.6044331151297119	2.099349761607257
5	0.25911646415634565	1.1249716338308204
6	0.13106961035405834	0.6828570813621752
7	0.07625106358918796	0.4634688012326066
8	0.048273969794731156	0.3353355216438009
9	0.030998267928472118	0.24224593375695244
>10	0.09559716849248465	1.2430847484623067
>50	0.001453076304733088	0.08340535574881533
>100	2.458802140173928E-4	0.03431428193423372
>500	4.08633829942011E-5	0.018752322966966054
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.653867986751834E-5	0.0	0.0	0.0
3	0.0	4.653867986751834E-5	0.0	0.0	0.0
4	0.0	9.307735973503668E-5	0.0	0.0	0.0
5	0.0	1.0471202970191627E-4	0.0	0.0	0.0
6	0.0	1.0471202970191627E-4	0.0	0.0	0.0
7	0.0	1.0471202970191627E-4	0.0	0.0	0.0
8	0.0	1.0471202970191627E-4	0.0	0.0	1.1634669966879586E-5
9	0.0	1.628853795363142E-4	0.0	0.0	1.1634669966879586E-5
10-11	5.817334983439793E-6	1.628853795363142E-4	0.0	0.0	1.1634669966879586E-5
12-13	1.1634669966879586E-5	1.6870271451975398E-4	0.0	5.817334983439793E-6	1.1634669966879586E-5
14-15	2.326933993375917E-5	2.559627392713509E-4	0.0	5.817334983439793E-5	3.490400990063876E-5
16-17	3.490400990063876E-5	2.6759740923823044E-4	0.0	8.726002475159689E-5	3.490400990063876E-5
18-19	3.490400990063876E-5	3.0250141913886917E-4	0.0	9.889469471847648E-5	3.490400990063876E-5
20-21	3.490400990063876E-5	3.490400990063875E-4	0.0	1.512507095694346E-4	3.490400990063876E-5
22-23	3.490400990063876E-5	4.0721344884078547E-4	0.0	4.188481188076651E-4	5.817334983439793E-5
24-25	3.490400990063876E-5	4.4793479372486403E-4	0.0	9.54042937284126E-4	8.726002475159689E-5
26-27	3.490400990063876E-5	4.886561386089425E-4	0.0	0.0017510178300153775	9.307735973503668E-5
28-29	3.490400990063876E-5	4.886561386089425E-4	0.0	0.007748690197941804	9.307735973503668E-5
30-31	3.490400990063876E-5	5.293774834930212E-4	0.0	0.013397322466861842	9.889469471847648E-5
32-33	4.653867986751834E-5	5.468294884433405E-4	0.0	0.02098312728526733	1.1634669966879586E-4
34-35	6.980801980127751E-5	5.991855032942987E-4	0.0	0.03108202081651881	1.2798136963567544E-4
36-37	6.980801980127751E-5	6.515415181452568E-4	0.0	0.04592785969425717	1.2798136963567544E-4
38-39	6.980801980127751E-5	6.573588531286966E-4	0.0	0.07583477884412113	1.2798136963567544E-4
40-41	6.980801980127751E-5	7.56253547847173E-4	0.0	0.11118672353848476	1.6870271451975398E-4
42-43	6.980801980127751E-5	7.678882178140526E-4	0.0	0.14238509105467234	1.803373844866336E-4
44-45	8.14426897681571E-5	8.493309075822097E-4	0.0	0.18114599404933168	1.8615471947007337E-4
46-47	9.889469471847648E-5	8.958695874497281E-4	0.0	0.22248397644165485	1.9197205445351315E-4
48-49	1.0471202970191627E-4	9.016869224331679E-4	0.0	0.26347291873497164	1.9778938943695295E-4
50-51	1.0471202970191627E-4	0.0010180336221019637	0.0	0.30378123283522596	1.9778938943695295E-4
52-53	1.0471202970191627E-4	0.0010238509570854035	0.0	0.37280972974872256	2.0942405940383254E-4
54-55	1.0471202970191627E-4	0.0010296682920688431	0.0	0.4552937224789153	2.2105872937071213E-4
56-57	1.0471202970191627E-4	0.001035485627052283	0.0	0.5436125021974982	2.2105872937071213E-4
58-59	1.0471202970191627E-4	0.0010587549669860423	0.0	0.6123675843667732	2.2105872937071213E-4
60-61	1.1634669966879586E-4	0.0010762069719363616	0.0	0.6643571071137746	2.2105872937071213E-4
62-63	1.2798136963567544E-4	0.001093658976886681	0.0	0.7096566946298203	2.326933993375917E-4
64-65	1.2798136963567544E-4	0.001210005676555477	0.0	0.7241883974184529	2.326933993375917E-4
66-67	1.3379870461911525E-4	0.0012216403465223563	0.0	0.7329318518985629	2.326933993375917E-4
68-69	1.3961603960255503E-4	0.0012216403465223563	0.0	0.741861461098143	2.326933993375917E-4
70-71	1.454333745859948E-4	0.0012274576815057962	0.0	0.7479987495056719	2.7923207920511005E-4
72-73	1.512507095694346E-4	0.0012449096864561157	0.0	0.7551133501904188	3.141360891057488E-4
74-75	1.512507095694346E-4	0.0012681790263898746	0.0	0.7607387131194051	3.141360891057488E-4
76-77	1.628853795363142E-4	0.0013496217161580318	0.0	0.7655089278058258	3.141360891057488E-4
78-79	1.7452004950319378E-4	0.0013845257260586706	0.0	0.7686677407018335	3.141360891057488E-4
80-81	1.7452004950319378E-4	0.0013845257260586706	0.0	0.769732313003803	3.315880940560682E-4
82-83	1.7452004950319378E-4	0.00139616039602555	0.0	0.7715473215186361	3.4904009900638757E-4
84-85	1.7452004950319378E-4	0.0014077950659924297	0.0	0.7733448780285191	3.4904009900638757E-4
86-87	1.7452004950319378E-4	0.0014194297359593094	0.0	0.774240747615969	3.4904009900638757E-4
88	1.7452004950319378E-4	0.0014776030857937073	0.0	0.7743338249757039	3.4904009900638757E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10755	0.0	46.272316	1
GTATCAA	19420	0.0	35.49879	1
ATCAACG	22760	0.0	29.44683	3
TCAACGC	22835	0.0	29.366486	4
CAACGCA	23315	0.0	28.671356	5
TATCAAC	24115	0.0	28.10351	2
AACGCAG	23980	0.0	27.929369	6
GTACATG	26940	0.0	24.785187	1
TACATGG	26555	0.0	24.62657	2
ACGCAGA	27500	0.0	24.29379	7
CGCAGAG	27950	0.0	23.918123	8
ACATGGG	26695	0.0	23.779062	3
CATGGGG	19645	0.0	21.172663	4
GCAGAGT	32875	0.0	20.348814	9
GAGTACT	18790	0.0	18.965271	12-13
AGAGTAC	28950	0.0	18.11901	10-11
CAGAGTA	31945	0.0	17.953346	10-11
GTGGTAT	5210	0.0	17.279984	1
GTACTTT	21315	0.0	16.843147	14-15
ATGGGGG	11845	0.0	16.641994	5
>>END_MODULE