FastQCFastQC Report
Fri 27 May 2016
SRR2139356_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139356_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6293155
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72650.048.0140041
GTATCAA134400.037.2238921
TCAACGC161250.030.3946554
ATCAACG162650.030.1095033
CAACGCA164650.029.7176845
TATCAAC171000.028.9479582
AACGCAG170550.028.6903176
ACGCAGA196850.024.6302577
CGCAGAG198200.024.4425878
GTACATG187250.024.3105161
TACATGG185150.024.0818022
ACATGGG191350.022.9195273
CATGGGG141900.020.5748234
GCAGAGT235150.020.516149
GAGTACT137550.018.85019712-13
CAGAGTA223550.018.3290810-11
AGAGTAC208650.018.0712310-11
GTGGTAT31800.017.622291
ATGGGGG85100.016.7958815
GTACTTT158350.016.3953614-15
Produced by FastQC (version 0.11.5)