##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139356_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6293155
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55970637939158	33.0	33.0	33.0	33.0	33.0
2	32.55687473135494	33.0	33.0	33.0	33.0	33.0
3	32.63469499797796	33.0	33.0	33.0	33.0	33.0
4	36.553170071291746	37.0	37.0	37.0	37.0	37.0
5	36.55773534896248	37.0	37.0	37.0	37.0	37.0
6	36.004691287597396	37.0	37.0	37.0	33.0	37.0
7	35.83946827942423	37.0	37.0	37.0	33.0	37.0
8	35.710772895312445	37.0	37.0	37.0	33.0	37.0
9	35.719840048433575	37.0	37.0	37.0	33.0	37.0
10-11	35.62404676191831	37.0	37.0	37.0	33.0	37.0
12-13	35.51933767720642	37.0	37.0	37.0	33.0	37.0
14-15	37.08551791589433	40.0	37.0	40.0	33.0	40.0
16-17	36.9964945246065	40.0	37.0	40.0	33.0	40.0
18-19	37.01577690363578	40.0	37.0	40.0	33.0	40.0
20-21	36.9222154688093	40.0	37.0	40.0	33.0	40.0
22-23	36.76485424575863	40.0	37.0	40.0	33.0	40.0
24-25	36.74998884878839	40.0	37.0	40.0	33.0	40.0
26-27	36.568717348968555	40.0	37.0	40.0	33.0	40.0
28-29	36.35556302076138	38.5	37.0	40.0	30.0	40.0
30-31	36.13560215723699	37.0	37.0	40.0	27.0	40.0
32-33	35.8951875386209	37.0	37.0	40.0	27.0	40.0
34-35	35.792622931848584	37.0	37.0	40.0	27.0	40.0
36-37	35.517033064010576	37.0	37.0	40.0	27.0	40.0
38-39	35.58099823471973	37.0	37.0	40.0	27.0	40.0
40-41	35.65258859479923	37.0	37.0	40.0	27.0	40.0
42-43	35.55838045750073	37.0	35.0	40.0	27.0	40.0
44-45	35.39545303925521	37.0	33.0	40.0	27.0	40.0
46-47	35.18233602563702	37.0	33.0	40.0	27.0	40.0
48-49	34.979030352071206	37.0	33.0	40.0	24.5	40.0
50-51	35.00466239865279	37.0	33.0	40.0	27.0	40.0
52-53	34.863586865577744	37.0	33.0	40.0	24.5	40.0
54-55	34.66399044590855	37.0	33.0	40.0	22.0	40.0
56-57	34.37203932367173	37.0	33.0	40.0	22.0	40.0
58-59	34.21002197421082	37.0	33.0	40.0	22.0	40.0
60-61	34.0695790648289	37.0	33.0	40.0	22.0	40.0
62-63	33.89893008432796	37.0	33.0	40.0	22.0	40.0
64-65	33.74614643807371	37.0	33.0	38.5	22.0	40.0
66-67	33.499924813923585	37.0	33.0	37.0	22.0	40.0
68-69	33.32789917142486	37.0	33.0	37.0	22.0	40.0
70-71	33.07923659459354	37.0	33.0	37.0	22.0	40.0
72-73	32.754736736957256	37.0	33.0	37.0	22.0	40.0
74-75	32.365362182088376	37.0	33.0	37.0	22.0	38.5
76-77	30.549078288296457	33.0	27.0	35.0	22.0	37.0
78-79	32.640757263778234	33.0	33.0	37.0	24.5	37.0
80-81	33.007000999588925	37.0	33.0	37.0	27.0	37.0
82-83	32.92451836965549	37.0	33.0	37.0	27.0	37.0
84-85	32.798948068411335	33.0	33.0	37.0	27.0	37.0
86-87	32.66111029512325	33.0	33.0	37.0	27.0	37.0
88-89	32.51008977429173	33.0	33.0	37.0	27.0	37.0
90-91	32.46357891336001	33.0	33.0	37.0	27.0	37.0
92-93	32.53844385664005	33.0	33.0	37.0	27.0	37.0
94-95	32.63190808358743	33.0	33.0	37.0	27.0	37.0
96-97	32.84145947075105	33.0	33.0	37.0	27.0	37.0
98-99	33.07862936615949	33.0	33.0	37.0	27.0	37.0
100	33.04527340886318	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	2.0
14	10.0
15	11.0
16	53.0
17	124.0
18	414.0
19	1032.0
20	2582.0
21	6732.0
22	15960.0
23	34329.0
24	65041.0
25	107609.0
26	154748.0
27	202123.0
28	241260.0
29	268670.0
30	285371.0
31	296718.0
32	308677.0
33	332129.0
34	377924.0
35	463162.0
36	668124.0
37	1284349.0
38	1144231.0
39	31769.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.77932134199777	19.971445165421795	13.261503967405854	23.987729525174576
2	15.14814254532881	21.56878105768705	40.719956752826256	22.56311964415788
3	18.126997868728147	26.888714937781227	30.46657246610337	24.517714727387258
4	12.451831415276843	17.891143209552453	39.09944729081605	30.557578084354653
5	13.045034817999818	38.40524773511783	35.59462164673256	12.955095800149783
6	30.067716431583204	37.92395070517093	18.917855987974235	13.090476875271625
7	26.553898640665928	32.6809366684914	23.562124244516465	17.203040446326206
8	25.06461385425911	36.05579395390706	21.52487901537464	17.354713176459185
9	26.468043453561847	16.016687972884824	20.934761657705874	36.58050691584746
10-11	24.508169167258117	26.415935626458282	29.22832139004595	19.847573816237656
12-13	25.45553544239943	24.119464996579936	29.082641182716085	21.342358378304553
14-15	22.588922447502725	25.28276992284803	26.629264610292484	25.49904301935676
16-17	22.165765820164925	28.15281841937788	27.305326183766333	22.376089576690866
18-19	22.203346334231398	27.42549643223471	28.960727965543516	21.41042926799038
20-21	23.066182772631414	26.704594012125334	28.661587162577757	21.56763605266549
22-23	22.72239081037831	26.715369125834492	28.498068519513943	22.064171544273258
24-25	22.48241141078228	26.85976614848326	28.624773018008025	22.03304942272643
26-27	22.44888292930416	27.037390430329726	28.511373264680962	22.00235337568515
28-29	22.36151699533638	27.077761010327865	28.466868853847778	22.09385314048798
30-31	22.643084421917116	26.992360977449852	28.339682556030976	22.024872044602056
32-33	22.45440058098097	27.1910389438402	28.26256196415264	22.091998511026187
34-35	22.52046459076705	27.215163304653338	28.28877736985503	21.97559473472458
36-37	22.615949859590785	27.17997590558962	28.19231343242151	22.011760802398076
38-39	22.41393100947278	27.337391296921265	28.26942760606536	21.979250087540592
40-41	22.59623018685731	27.168426167941867	28.19918859902358	22.036155046177246
42-43	22.4903115367342	27.234901589467693	28.025737396002278	22.24904947779583
44-45	22.556473009496887	27.347389753178415	27.77327666384664	22.32286057347806
46-47	22.62378846176408	27.255650699518114	27.551037931818385	22.56952290689942
48-49	22.44312659736111	27.387098332050375	27.63801002340605	22.531765047182468
50-51	22.47494544686744	27.491336316539538	27.52618615397489	22.507532082618134
52-53	22.605711617554878	27.389629611985722	27.408947746635043	22.595711023824357
54-55	22.51657196969697	27.32346788783356	27.490751144843962	22.66920899762551
56-57	22.502967215439305	27.47398619097402	27.370798885477093	22.65224770810958
58-59	22.401330311814423	27.461512913378357	27.486329735677344	22.650827039129876
60-61	22.4835094902474	27.54801695544244	27.468102358789626	22.500371195520536
62-63	22.391324051195525	27.67278805361099	27.405478202347737	22.530409692845748
64-65	22.342125552320134	27.608398091614195	27.408380499603712	22.641095856461963
66-67	22.413490942903717	27.70945321790656	27.247721328094425	22.629334511095294
68-69	22.36718652589319	27.66688906150411	27.28410985659599	22.681814556006714
70-71	22.36561027999013	27.449264940679726	27.287110918393726	22.89801386093642
72-73	22.364513286418187	27.448953097268248	27.26241451638927	22.92411909992429
74-75	22.307331039733054	27.47386512544237	27.223915660409048	22.99488817441553
76-77	22.34203680237936	27.398433391864245	27.308172780641872	22.951357025114525
78-79	22.306152020528426	27.412735061271054	27.291223165779332	22.98988975242119
80-81	22.349248463194254	27.474956740810818	27.25082533668305	22.924969459311882
82-83	22.34387669293327	27.408343162040683	27.32586262019457	22.92191752483148
84-85	22.355013662785282	27.4213859223309	27.338382696253067	22.885217718630752
86-87	22.243812835293408	27.464204022455853	27.37540806258705	22.916575079663698
88-89	22.21623560498469	27.331053810705804	27.51814692541124	22.934563658898263
90-91	22.21960874249351	27.28345694519377	27.640246905465414	22.856687406847307
92-93	22.24022699893904	27.257130015465936	27.659586329518138	22.843056656076886
94-95	22.063519502475856	27.32248655218666	27.781261060958112	22.83273288437937
96-97	22.03390741100399	27.45837863015951	27.942399897707748	22.565314061128756
98-99	23.183672660688607	29.28976386202317	28.191831317022224	19.334732160266
100	22.16498970775136	30.059640619647794	21.81057140387552	25.964798268725332
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1075.0
1	1013.5
2	1499.0
3	2250.0
4	3234.0
5	5739.0
6	7948.0
7	8778.5
8	8667.5
9	7821.5
10	6894.0
11	6176.0
12	6597.0
13	9153.0
14	14832.5
15	22855.5
16	30115.0
17	35026.5
18	37356.0
19	36240.0
20	33426.5
21	30613.0
22	28693.5
23	29330.5
24	32215.5
25	37789.0
26	46123.0
27	55644.5
28	66316.5
29	78542.0
30	91361.5
31	105517.0
32	121530.0
33	137180.5
34	153287.0
35	169898.5
36	186885.5
37	202992.5
38	216688.0
39	228737.0
40	241063.5
41	252131.0
42	259524.0
43	267103.0
44	274327.0
45	279901.0
46	283599.0
47	286066.5
48	286728.0
49	283608.5
50	279105.0
51	272299.0
52	262118.5
53	249359.0
54	234677.5
55	219151.0
56	203350.5
57	186092.0
58	166936.0
59	147381.0
60	126462.0
61	106129.5
62	88074.5
63	70963.5
64	55778.5
65	43665.5
66	33824.0
67	26603.5
68	21358.0
69	16423.5
70	12399.5
71	9545.0
72	7285.0
73	5360.0
74	3885.5
75	2859.5
76	2173.5
77	1618.5
78	1173.0
79	845.0
80	601.0
81	437.0
82	330.5
83	235.5
84	163.5
85	123.5
86	94.0
87	68.5
88	49.0
89	33.5
90	22.5
91	17.5
92	17.5
93	13.0
94	6.5
95	4.5
96	4.0
97	3.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.2903758130858054E-4
3	0.0031621658770521303
4	0.002431212960748623
5	3.178056157841337E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.668479482866702E-4
12-13	0.002359706697197193
14-15	0.00294764708639784
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.005346795038620435
26-27	0.016530961052576603
28-29	0.006940168614041115
30-31	0.0
32-33	0.0
34-35	1.623929576669978E-4
36-37	0.018668212800647406
38-39	0.05179948420485083
40-41	0.05910782622315612
42-43	0.010901664240234369
44-45	4.875132311511205E-4
46-47	8.501574534111591E-6
48-49	0.034592686365628116
50-51	0.03869357522827606
52-53	0.046653499817762505
54-55	0.03009609556146174
56-57	0.0010536495115263007
58-59	0.0059824302710248765
60-61	0.02729898797901922
62-63	0.039435906276867855
64-65	0.02408229876897783
66-67	0.06441501258570245
68-69	0.07959795728562163
70-71	0.0737678562325751
72-73	0.04568291038348833
74-75	0.021275470488529444
76-77	0.005396609015269553
78-79	4.5374685071326425E-4
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0023508770330588485
92-93	0.0029360138520390243
94-95	8.090969206927052E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2058.0
20-21	6212.0
22-23	7099.0
24-25	12799.0
26-27	17785.0
28-29	26375.0
30-31	25959.0
32-33	28598.0
34-35	32378.0
36-37	33584.0
38-39	36589.0
40-41	47541.0
42-43	52141.0
44-45	64277.0
46-47	65859.0
48-49	50299.0
50-51	59354.0
52-53	47952.0
54-55	58796.0
56-57	63626.0
58-59	72835.0
60-61	66288.0
62-63	65941.0
64-65	75713.0
66-67	82945.0
68-69	71479.0
70-71	66732.0
72-73	62405.0
74-75	64747.0
76-77	55416.0
78-79	86268.0
80-81	101909.0
82-83	116372.0
84-85	122272.0
86-87	120798.0
88-89	120180.0
90-91	131465.0
92-93	139999.0
94-95	156174.0
96-97	270487.0
98-99	379739.0
100-101	3123710.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.40108257965414
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.26140651747563	84.3093211208101
2	4.9793291606521795	9.002734932867952
3	1.0578965453522038	2.8690497887134625
4	0.3322179021029153	1.201314320097804
5	0.14844821969163924	0.6709939883573259
6	0.07683845240319642	0.41677675685965104
7	0.042252424608036306	0.26737654483271905
8	0.026836780792045243	0.19408592292430063
9	0.015849731129156115	0.1289549567404941
>10	0.057812231382891405	0.822432726881893
>50	8.181777413687492E-4	0.048803220781923055
>100	2.701866370669803E-4	0.05499597168893027
>500	2.3670031749601024E-5	0.013159748443381114
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5890280789206687E-5
2	0.0	4.767084236762006E-5	0.0	0.0	1.5890280789206687E-5
3	0.0	4.767084236762006E-5	0.0	0.0	1.5890280789206687E-5
4	0.0	2.224639310488936E-4	0.0	1.5890280789206687E-5	3.1780561578413374E-5
5	0.0	2.3835421183810029E-4	0.0	1.5890280789206687E-5	3.1780561578413374E-5
6	1.5890280789206687E-5	2.3835421183810029E-4	0.0	1.5890280789206687E-5	3.1780561578413374E-5
7	1.5890280789206687E-5	2.3835421183810029E-4	0.0	3.1780561578413374E-5	3.1780561578413374E-5
8	1.5890280789206687E-5	2.7013477341651367E-4	0.0	3.1780561578413374E-5	4.767084236762006E-5
9	1.5890280789206687E-5	3.8136673894096046E-4	0.0	6.356112315682675E-5	4.767084236762006E-5
10-11	4.767084236762006E-5	3.8136673894096046E-4	0.0	6.356112315682675E-5	4.767084236762006E-5
12-13	4.767084236762006E-5	3.8136673894096046E-4	0.0	6.356112315682675E-5	5.56159827622234E-5
14-15	4.767084236762006E-5	5.482146872276307E-4	0.0	8.739654434063678E-5	7.945140394603344E-5
16-17	6.356112315682675E-5	5.79995248806044E-4	0.0	1.0328682512984345E-4	9.534168473524011E-5
18-19	6.356112315682675E-5	6.356112315682675E-4	0.0	1.271222463136535E-4	1.112319655244468E-4
20-21	6.356112315682675E-5	6.912272143304908E-4	0.0	1.668479482866702E-4	1.271222463136535E-4
22-23	7.945140394603342E-5	7.945140394603344E-4	0.0	3.2575075617873706E-4	1.271222463136535E-4
24-25	1.1123196552444679E-4	8.898557241955744E-4	0.0	6.197209507790608E-4	1.3506738670825683E-4
26-27	1.7479308868127355E-4	8.898557241955744E-4	0.0	0.0012314967611635182	1.4301252710286017E-4
28-29	1.9068336947048023E-4	8.898557241955744E-4	0.0	0.003932844495328654	1.4301252710286017E-4
30-31	1.9068336947048023E-4	0.0010090328301146246	0.0	0.007452541690137936	1.4301252710286017E-4
32-33	1.9068336947048023E-4	0.001016977970509228	0.0	0.016057128737493355	1.4301252710286017E-4
34-35	1.9862850986508357E-4	0.001136155076428278	0.0	0.028713737386096484	1.5890280789206685E-4
36-37	2.065736502596869E-4	0.0012553321823473283	0.0	0.04861631407457785	1.827382290758769E-4
38-39	2.065736502596869E-4	0.0012950578843203449	0.0	0.08812749725694027	2.065736502596869E-4
40-41	2.065736502596869E-4	0.001414234990239395	0.0	0.1361638160827121	2.065736502596869E-4
42-43	2.065736502596869E-4	0.0014460155518178084	0.0	0.17817771848937458	2.065736502596869E-4
44-45	2.065736502596869E-4	0.0015651926577368584	0.0	0.23268932673674808	2.065736502596869E-4
46-47	2.3835421183810029E-4	0.0016605343424720986	0.0	0.29323129654362556	2.3040907144349695E-4
48-49	2.3835421183810029E-4	0.001668479482866702	0.0	0.352811904362756	2.54244492627307E-4
50-51	2.54244492627307E-4	0.0020101205198346456	0.0	0.4057027039696305	2.54244492627307E-4
52-53	2.54244492627307E-4	0.0020498462218076626	0.0	0.4880699744404834	2.9397019460032365E-4
54-55	2.54244492627307E-4	0.002065736502596869	0.0	0.5811075684612885	3.336958965733404E-4
56-57	2.54244492627307E-4	0.0020975170641752824	0.0	0.6842196005024507	3.336958965733404E-4
58-59	2.621896330219103E-4	0.0021213524853590923	0.0	0.7807053854545136	3.495861773625471E-4
60-61	2.7013477341651367E-4	0.002129297625753696	0.0	0.831737022209051	3.8136673894096046E-4
62-63	2.7013477341651367E-4	0.002169023327726713	0.0	0.8880839578875779	3.8136673894096046E-4
64-65	2.7013477341651367E-4	0.002478883803116243	0.0	0.9125232097413778	3.8136673894096046E-4
66-67	2.7013477341651367E-4	0.0024868289435108467	0.0	0.9215568343700418	3.8136673894096046E-4
68-69	2.7013477341651367E-4	0.0025186095050892597	0.0	0.9293271816759638	3.8136673894096046E-4
70-71	2.7013477341651367E-4	0.0025424449262730696	0.0	0.9350556279004728	4.131473005193738E-4
72-73	3.178056157841337E-4	0.0025583352070622763	0.0	0.942063241728513	4.290375813085805E-4
74-75	3.178056157841337E-4	0.0025901157686406898	0.0	0.9480062067436763	4.290375813085805E-4
76-77	3.178056157841337E-4	0.00268545745337593	0.0	0.953099041736617	4.290375813085805E-4
78-79	3.178056157841337E-4	0.002717238014954343	0.0	0.9563009333156421	4.290375813085805E-4
80-81	3.178056157841337E-4	0.002717238014954343	0.0	0.9576516071827248	4.6876328328159726E-4
82-83	3.178056157841337E-4	0.002717238014954343	0.0	0.960448296601625	5.164341256492172E-4
84-85	3.178056157841337E-4	0.0027490185765327566	0.0	0.9628477290007953	5.32324406438424E-4
86-87	3.178056157841337E-4	0.0027887442785057732	0.0	0.9642619639910347	5.402695468330273E-4
88	3.178056157841337E-4	0.002892031103635617	0.0	0.9644447022201106	5.402695468330273E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7265	0.0	48.014004	1
GTATCAA	13440	0.0	37.223892	1
TCAACGC	16125	0.0	30.394655	4
ATCAACG	16265	0.0	30.109503	3
CAACGCA	16465	0.0	29.717684	5
TATCAAC	17100	0.0	28.947958	2
AACGCAG	17055	0.0	28.690317	6
ACGCAGA	19685	0.0	24.630257	7
CGCAGAG	19820	0.0	24.442587	8
GTACATG	18725	0.0	24.310516	1
TACATGG	18515	0.0	24.081802	2
ACATGGG	19135	0.0	22.919527	3
CATGGGG	14190	0.0	20.574823	4
GCAGAGT	23515	0.0	20.51614	9
GAGTACT	13755	0.0	18.850197	12-13
CAGAGTA	22355	0.0	18.32908	10-11
AGAGTAC	20865	0.0	18.07123	10-11
GTGGTAT	3180	0.0	17.62229	1
ATGGGGG	8510	0.0	16.795881	5
GTACTTT	15835	0.0	16.39536	14-15
>>END_MODULE