FastQCFastQC Report
Fri 27 May 2016
SRR2139355_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139355_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6783769
Sequences flagged as poor quality0
Sequence length19-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72750.047.2755131
GTATCAA138350.038.8948061
TCAACGC170050.030.7246464
ATCAACG171950.030.3853683
CAACGCA174300.029.8806295
TATCAAC179050.029.4791742
AACGCAG180850.028.844766
GTACATG215600.025.9700261
TACATGG213200.025.8764442
ACGCAGA212100.024.4979027
ACATGGG219500.024.4777583
CGCAGAG218150.023.8382328
CATGGGG169350.021.960544
GCAGAGT254800.020.4258299
GAGTACT145450.018.52719912-13
AGAGTAC223850.018.50806410-11
ATGGGGG101250.018.1620925
GTGGTAT38350.017.8076131
CAGAGTA241600.017.55700110-11
GTATAAT36450.016.8171231
Produced by FastQC (version 0.11.5)