##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139355_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6783769
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.58388928042803	33.0	33.0	33.0	33.0	33.0
2	32.581683869247314	33.0	33.0	33.0	33.0	33.0
3	32.654005317692864	33.0	33.0	33.0	33.0	33.0
4	36.573823636978204	37.0	37.0	37.0	37.0	37.0
5	36.575675557348724	37.0	37.0	37.0	37.0	37.0
6	36.05736280230061	37.0	37.0	37.0	33.0	37.0
7	35.89947652993491	37.0	37.0	37.0	33.0	37.0
8	35.781657807039124	37.0	37.0	37.0	33.0	37.0
9	35.784984129029155	37.0	37.0	37.0	33.0	37.0
10-11	35.69325834945146	37.0	37.0	37.0	33.0	37.0
12-13	35.591669542403345	37.0	37.0	37.0	33.0	37.0
14-15	37.21071413251247	40.0	37.0	40.0	33.0	40.0
16-17	37.126491040599994	40.0	37.0	40.0	33.0	40.0
18-19	37.14478699083061	40.0	37.0	40.0	33.0	40.0
20-21	37.053764617875025	40.0	37.0	40.0	33.0	40.0
22-23	36.90436856608402	40.0	37.0	40.0	33.0	40.0
24-25	36.88544882511974	40.0	37.0	40.0	33.0	40.0
26-27	36.71265234320086	40.0	37.0	40.0	33.0	40.0
28-29	36.51092369299987	40.0	37.0	40.0	33.0	40.0
30-31	36.301197628108056	38.5	37.0	40.0	30.0	40.0
32-33	36.07243960079062	37.0	37.0	40.0	27.0	40.0
34-35	35.973212374174224	37.0	37.0	40.0	27.0	40.0
36-37	35.71346506052815	37.0	37.0	40.0	27.0	40.0
38-39	35.766839007660806	37.0	37.0	40.0	27.0	40.0
40-41	35.83664946015115	37.0	37.0	40.0	27.0	40.0
42-43	35.743511338353755	37.0	37.0	40.0	27.0	40.0
44-45	35.58902546373544	37.0	35.0	40.0	27.0	40.0
46-47	35.39193760624856	37.0	33.0	40.0	27.0	40.0
48-49	35.19473420462033	37.0	33.0	40.0	27.0	40.0
50-51	35.2138039797507	37.0	33.0	40.0	27.0	40.0
52-53	35.08473939561827	37.0	33.0	40.0	27.0	40.0
54-55	34.885609791707424	37.0	33.0	40.0	27.0	40.0
56-57	34.61120397654841	37.0	33.0	40.0	27.0	40.0
58-59	34.466033192844705	37.0	33.0	40.0	24.5	40.0
60-61	34.337436257043024	37.0	33.0	40.0	22.0	40.0
62-63	34.16538945820943	37.0	33.0	40.0	22.0	40.0
64-65	34.00612031136327	37.0	33.0	38.5	22.0	40.0
66-67	33.75555174655569	37.0	33.0	37.0	22.0	40.0
68-69	33.569638240690466	37.0	33.0	37.0	22.0	40.0
70-71	33.33150416865074	37.0	33.0	37.0	22.0	40.0
72-73	33.017230320936015	37.0	33.0	37.0	22.0	40.0
74-75	32.64602028362944	37.0	33.0	37.0	22.0	37.0
76-77	30.71971890682075	33.0	27.0	35.0	22.0	37.0
78-79	32.826812591808476	35.0	33.0	37.0	27.0	37.0
80-81	33.21158993602414	37.0	33.0	37.0	27.0	37.0
82-83	33.166059271957174	37.0	33.0	37.0	27.0	37.0
84-85	33.06966666530993	37.0	33.0	37.0	27.0	37.0
86-87	32.94057333607062	35.0	33.0	37.0	27.0	37.0
88-89	32.785137963804665	33.0	33.0	37.0	27.0	37.0
90-91	32.71613092078934	33.0	33.0	37.0	27.0	37.0
92-93	32.78015009187333	33.0	33.0	37.0	27.0	37.0
94-95	32.86174629110662	33.0	33.0	37.0	27.0	37.0
96-97	33.02841374321841	33.0	33.0	37.0	27.0	37.0
98-99	33.230104448703514	33.0	33.0	37.0	27.0	37.0
100	33.202295970949756	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	2.0
15	13.0
16	44.0
17	131.0
18	394.0
19	1058.0
20	2588.0
21	6569.0
22	15836.0
23	34940.0
24	65619.0
25	108006.0
26	156826.0
27	205633.0
28	246180.0
29	277367.0
30	294819.0
31	308479.0
32	322097.0
33	350952.0
34	401478.0
35	502614.0
36	755536.0
37	1502446.0
38	1202755.0
39	21385.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.30047843617294	19.506548573005446	13.070243557857522	24.1227294329641
2	15.47206436099518	21.23810537438367	40.29545076636237	22.994379498258777
3	18.547540766591922	26.473397363654495	30.00129578037009	24.97776608938349
4	12.750849660099107	17.483325581789597	38.33748919267733	31.42833556543396
5	13.473150459494033	38.214260338578896	35.06317877120172	13.249410430725353
6	30.911709994842102	37.18316469797247	18.506673797412617	13.398451509772812
7	27.200513460879932	32.27517033672579	22.997304300898218	17.527011901496056
8	25.632609247160392	35.60205248734148	21.072843134841413	17.69249513065672
9	26.855425059432303	15.735353016884861	20.37293722707834	37.0362846966045
10-11	25.14612654736263	25.998566724164274	28.67674205184573	20.178564676627367
12-13	25.966823952194318	23.68925983422737	28.550367362366075	21.793548851212236
14-15	23.109969067832154	24.792656591612094	26.06326655072939	26.034107789826365
16-17	22.733159103737172	27.68126391096159	26.7101665755423	22.87541040975894
18-19	22.79236660328499	26.950925068350646	28.328713728312387	21.927994600051978
20-21	23.740055720635432	26.22037402902438	27.999785241694177	22.039785008646014
22-23	23.32000530020924	26.1970371239795	27.89031371997548	22.592643855835785
24-25	23.057207456452097	26.378635275436537	28.059571187102865	22.504586081008505
26-27	23.04277586747155	26.56556628137733	27.934696053842558	22.45696179730857
28-29	22.9803897080442	26.581013116025176	27.89738820602199	22.54120896990863
30-31	23.267856286043536	26.475636373187903	27.778123109047016	22.47838423172154
32-33	23.046418734660588	26.6700301802167	27.77852117387722	22.505029911245497
34-35	23.128748251073368	26.68309110708919	27.788372086370668	22.399788555466777
36-37	23.171677734427032	26.667096541385433	27.6233407441313	22.537884980056237
38-39	22.985636322358467	26.834061389616497	27.723688655800604	22.456613632224432
40-41	23.246644962733036	26.643616606242826	27.60327177725721	22.506466653766925
42-43	23.056567174179538	26.718502799124938	27.50706286837617	22.717867158319358
44-45	23.096946041478393	26.841280492750606	27.265673184576723	22.796100281194278
46-47	23.21008632126484	26.711507991332294	27.03157913256487	23.046826554837992
48-49	23.030236283079816	26.922408147837864	27.08149431578557	22.96586125329675
50-51	23.041105025021196	27.028785372564535	26.95069563223812	22.97941397017615
52-53	23.246295112552822	26.936064361920366	26.802549227849052	23.01509129767776
54-55	23.115554551756436	26.831949687502345	26.897082562151624	23.155413198589596
56-57	23.172587351200878	27.03212244343244	26.770165357375138	23.025124847991545
58-59	23.08651757877555	27.085360657228623	26.835678947946935	22.992442816048893
60-61	23.060325212033177	27.13531748575328	26.869385408712215	22.934971893501327
62-63	23.097132060252758	27.324555158687712	26.722182558146663	22.856130222912864
64-65	23.040110039525256	27.159793837470303	26.80583015587672	22.99426596712772
66-67	23.0207881008899	27.190953130637936	26.7911132235204	22.99714554495176
68-69	22.996997024040493	27.199477046211644	26.830163888342234	22.97336204140563
70-71	23.066461608516015	26.891060997359396	26.890873498614965	23.151603895509627
72-73	23.041385000918474	26.935293658503763	26.824281002210753	23.19904033836701
74-75	22.892984309596823	27.081496070124878	26.793258867404663	23.232260752873636
76-77	22.96940448888525	26.951849698020265	26.88413171466273	23.19461409843176
78-79	22.80832018230214	26.995973272469016	26.920203572463524	23.27550297276532
80-81	22.892153187875255	27.001932985244103	26.926578041336786	23.179335785543856
82-83	22.868366969491856	26.984153478499106	27.039427133935302	23.108052418073736
84-85	22.902244678800507	26.992005621989833	26.95369965520718	23.152050044002475
86-87	22.76806600995001	27.022617951428263	26.997607201413267	23.211708837208462
88-89	22.783883507573528	26.899154307888896	27.061159717535993	23.255802467001587
90-91	22.78592922212232	26.965973033539868	27.121717783693327	23.126379960644485
92-93	22.864677428054627	26.880041875642707	27.107141242725646	23.14813945357702
94-95	22.684321140774262	26.934478588518378	27.21301175033898	23.16818852036838
96-97	22.609255238702737	27.07868953168346	27.378459400443777	22.933595829170024
98-99	23.836634198814952	28.900701339683998	27.540709363235482	19.72195509826556
100	22.738496785568152	29.56175561057749	21.273740062372447	26.426007541481916
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	974.0
1	913.0
2	1370.5
3	2116.0
4	3107.0
5	5481.5
6	7537.5
7	8350.0
8	8271.5
9	7457.5
10	6516.0
11	5764.0
12	6157.5
13	8593.0
14	14122.5
15	22332.0
16	30050.0
17	34991.5
18	36664.5
19	34775.0
20	31555.0
21	28281.0
22	25790.5
23	25674.5
24	27629.5
25	32146.5
26	39146.0
27	47421.0
28	56700.0
29	67786.0
30	79832.0
31	92648.0
32	107681.0
33	123023.0
34	139445.0
35	156602.0
36	174934.5
37	193670.0
38	209726.0
39	225315.5
40	241295.0
41	255126.0
42	266928.5
43	278677.0
44	289418.0
45	299612.5
46	307389.5
47	312572.5
48	316720.0
49	316529.0
50	313806.0
51	309546.0
52	300613.5
53	287969.5
54	273850.5
55	257658.0
56	240301.0
57	221415.0
58	199836.0
59	177250.5
60	152825.5
61	128438.0
62	106242.5
63	85618.5
64	67268.5
65	52807.5
66	41284.5
67	32707.0
68	26147.0
69	19996.0
70	15234.5
71	11676.0
72	8807.0
73	6524.5
74	4806.5
75	3562.0
76	2643.5
77	1945.0
78	1428.5
79	1064.5
80	783.5
81	564.0
82	428.0
83	297.0
84	204.0
85	162.5
86	138.0
87	107.0
88	68.0
89	42.5
90	27.5
91	17.0
92	13.5
93	7.5
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.4741067981530622E-5
2	4.8645524339051055E-4
3	0.0031251064120844914
4	0.002564945828786328
5	4.4223203944591866E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.2529907784301027E-4
12-13	0.002063749517414287
14-15	0.003184070684010614
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.005743591017289762
26-27	0.018468603818000466
28-29	0.008191501547576828
30-31	0.0
32-33	0.0
34-35	1.355025790657549E-4
36-37	0.01694993232134166
38-39	0.0469210317058526
40-41	0.053494839278620185
42-43	0.009637001893246844
44-45	3.0328669457941134E-4
46-47	0.0
48-49	0.033425146140889726
50-51	0.04229071592848751
52-53	0.047016764871928365
54-55	0.028916042636745593
56-57	8.64320029554806E-4
58-59	0.005849167687566916
60-61	0.027583242104002613
62-63	0.036816691777211086
64-65	0.020992418067915585
66-67	0.05539679149938935
68-69	0.07065193081016184
70-71	0.0712166256827566
72-73	0.045617918023896156
74-75	0.021022502310828584
76-77	0.004883580608067638
78-79	4.3808098159966666E-4
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0019165936560961764
92-93	0.0020090121665340063
94-95	4.629964225056915E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2294.0
20-21	6538.0
22-23	7619.0
24-25	13672.0
26-27	18848.0
28-29	27068.0
30-31	27167.0
32-33	29719.0
34-35	34156.0
36-37	34867.0
38-39	37916.0
40-41	45334.0
42-43	53814.0
44-45	61369.0
46-47	62274.0
48-49	51658.0
50-51	63074.0
52-53	53834.0
54-55	59200.0
56-57	66936.0
58-59	64147.0
60-61	73994.0
62-63	55744.0
64-65	68127.0
66-67	81116.0
68-69	59128.0
70-71	77691.0
72-73	66653.0
74-75	53577.0
76-77	42650.0
78-79	75225.0
80-81	76093.0
82-83	92091.0
84-85	116524.0
86-87	130542.0
88-89	135295.0
90-91	142287.0
92-93	148769.0
94-95	165247.0
96-97	295033.0
98-99	421242.0
100-101	3585237.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.50495830514913
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.2603734755588	81.65500507671818
2	5.519558664765489	9.770166189757882
3	1.2437837899735213	3.3024309741668056
4	0.4600420982383522	1.6286402689279473
5	0.19316081317623676	0.8547844858175767
6	0.1038628209364387	0.5515424781860806
7	0.06740160434120371	0.4175763327342875
8	0.03691483729750329	0.26137169086855144
9	0.026503603722157893	0.21111303081292015
>10	0.0861796884789874	1.1556752156147805
>50	0.0018123700803925778	0.10004059857526096
>100	3.575688073143507E-4	0.06365776642062214
>500	4.866462365815346E-5	0.027995891399111985
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.896427192612248E-5	0.0	0.0	0.0
3	0.0	5.896427192612248E-5	0.0	0.0	0.0
4	0.0	1.7689281577836747E-4	0.0	0.0	0.0
5	0.0	1.7689281577836747E-4	0.0	0.0	0.0
6	0.0	1.7689281577836747E-4	0.0	0.0	0.0
7	0.0	1.7689281577836747E-4	0.0	1.474106798153062E-5	0.0
8	0.0	2.5059815568602054E-4	0.0	1.474106798153062E-5	0.0
9	0.0	3.8326776751979616E-4	0.0	2.948213596306124E-5	0.0
10-11	1.474106798153062E-5	3.8326776751979616E-4	0.0	4.422320394459186E-5	0.0
12-13	3.685266995382655E-5	3.8326776751979616E-4	0.0	7.37053399076531E-5	0.0
14-15	5.1593737935357174E-5	5.085668453628065E-4	0.0	8.844640788918373E-5	3.685266995382655E-5
16-17	5.896427192612248E-5	5.159373793535717E-4	0.0	8.844640788918373E-5	6.63348059168878E-5
18-19	5.896427192612248E-5	5.527900493073983E-4	0.0	1.0318747587071435E-4	8.844640788918373E-5
20-21	7.37053399076531E-5	5.896427192612248E-4	0.0	1.326696118337756E-4	1.1055800986147966E-4
22-23	7.37053399076531E-5	6.559775251781127E-4	0.0	1.4004014582454089E-4	1.326696118337756E-4
24-25	7.37053399076531E-5	7.591650010488271E-4	0.0	1.6952228178760214E-4	1.5478121380607153E-4
26-27	7.37053399076531E-5	7.81276603021123E-4	0.0	2.727097576583165E-4	1.6215174779683682E-4
28-29	7.37053399076531E-5	7.81276603021123E-4	0.0	7.002007291227045E-4	1.6215174779683682E-4
30-31	7.37053399076531E-5	8.402408749472454E-4	0.0	0.001614146943977603	1.6952228178760214E-4
32-33	9.581694187994904E-5	8.402408749472454E-4	0.0	0.0037294901993272472	1.7689281577836747E-4
34-35	1.326696118337756E-4	9.72910486781021E-4	0.0	0.006346029766048932	1.9163388375989808E-4
36-37	1.6215174779683682E-4	0.0011055800986147966	0.0	0.010746238558535823	1.9163388375989808E-4
38-39	1.6215174779683682E-4	0.0011350622345778578	0.0	0.019340281191768175	2.063749517414287E-4
40-41	1.6215174779683682E-4	0.001208767574485511	0.0	0.02824388625261267	2.211160197229593E-4
42-43	1.6215174779683682E-4	0.0012382497104485722	0.0	0.03639569684639911	2.2848655371372463E-4
44-45	1.6215174779683682E-4	0.0013193255843469906	0.0	0.04640488200585839	2.5059815568602054E-4
46-47	1.6215174779683682E-4	0.0013709193222823477	0.0	0.05583916551403799	2.948213596306124E-4
48-49	1.6215174779683682E-4	0.0013709193222823477	0.0	0.06362244940828617	3.2430349559367365E-4
50-51	1.6215174779683682E-4	0.0015772942740237765	0.0	0.07133202796262667	3.2430349559367365E-4
52-53	1.990044177506634E-4	0.0015994058759960725	0.0	0.08017666875154505	3.390445635752043E-4
54-55	2.063749517414287E-4	0.0016067764099868377	0.0	0.08745875633442117	3.6115616554750025E-4
56-57	2.063749517414287E-4	0.0016215174779683683	0.0	0.09440179935372209	3.9063830151056143E-4
58-59	2.063749517414287E-4	0.0016215174779683683	0.0	0.10035719081826047	4.127499034828574E-4
60-61	2.063749517414287E-4	0.0016362585459498988	0.0	0.10537652446597165	4.127499034828574E-4
62-63	2.063749517414287E-4	0.0016952228178760214	0.0	0.11189207651380817	4.2749097146438803E-4
64-65	2.063749517414287E-4	0.0017689281577836745	0.0	0.11711041457927002	4.2749097146438803E-4
66-67	2.063749517414287E-4	0.0017762986917744398	0.0	0.12277098468417777	4.2749097146438803E-4
68-69	2.211160197229593E-4	0.0017910397597559705	0.0	0.1289696037704114	4.2749097146438803E-4
70-71	2.211160197229593E-4	0.0017984102937467358	0.0	0.1331339554751938	4.4960257343668394E-4
72-73	2.211160197229593E-4	0.001805780827737501	0.0	0.13890508358996304	5.23307913344337E-4
74-75	2.211160197229593E-4	0.0018426334976913276	0.0	0.14334214505240378	5.380489813258677E-4
76-77	2.3585708770448993E-4	0.0019163388375989807	0.0	0.1472632691354909	5.45419515316633E-4
78-79	2.5059815568602054E-4	0.0019310799055805114	0.0	0.14991666137216642	5.45419515316633E-4
80-81	2.5059815568602054E-4	0.0019310799055805114	0.0	0.15130969229642105	5.896427192612248E-4
82-83	2.5059815568602054E-4	0.001945820973562042	0.0	0.15362403996952137	6.338659232058168E-4
84-85	2.5059815568602054E-4	0.0019605620415435727	0.0	0.1555035261371665	6.338659232058168E-4
86-87	2.5059815568602054E-4	0.0019605620415435727	0.0	0.15666807050770742	6.338659232058168E-4
88	2.5059815568602054E-4	0.002034267381451226	0.0	0.15683022225550428	6.338659232058168E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7275	0.0	47.275513	1
GTATCAA	13835	0.0	38.894806	1
TCAACGC	17005	0.0	30.724646	4
ATCAACG	17195	0.0	30.385368	3
CAACGCA	17430	0.0	29.880629	5
TATCAAC	17905	0.0	29.479174	2
AACGCAG	18085	0.0	28.84476	6
GTACATG	21560	0.0	25.970026	1
TACATGG	21320	0.0	25.876444	2
ACGCAGA	21210	0.0	24.497902	7
ACATGGG	21950	0.0	24.477758	3
CGCAGAG	21815	0.0	23.838232	8
CATGGGG	16935	0.0	21.96054	4
GCAGAGT	25480	0.0	20.425829	9
GAGTACT	14545	0.0	18.527199	12-13
AGAGTAC	22385	0.0	18.508064	10-11
ATGGGGG	10125	0.0	18.162092	5
GTGGTAT	3835	0.0	17.807613	1
CAGAGTA	24160	0.0	17.557001	10-11
GTATAAT	3645	0.0	16.817123	1
>>END_MODULE