FastQCFastQC Report
Fri 27 May 2016
SRR2139354_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139354_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8853991
Sequences flagged as poor quality0
Sequence length19-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100050.044.905181
GTATCAA176000.039.0710561
TCAACGC217850.030.7302194
ATCAACG221250.030.2779353
CAACGCA222500.029.9543515
TATCAAC231100.029.426352
AACGCAG230250.029.0023256
GTACATG304250.027.2968331
TACATGG297950.027.2356722
ACATGGG306250.026.097313
ACGCAGA269400.024.6621157
CGCAGAG276750.024.023228
CATGGGG234900.023.299944
GCAGAGT329150.020.0330859
ATGGGGG134550.019.6315465
AGAGTAC284150.018.40833910-11
GAGTACT172950.018.06060812-13
CAGAGTA310050.017.5931410-11
GTGGTAT52250.017.5530971
GTATAAT52400.017.502851
Produced by FastQC (version 0.11.5)