##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139354_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8853991
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64270112766096	33.0	33.0	33.0	33.0	33.0
2	32.64094576106978	33.0	33.0	33.0	33.0	33.0
3	32.69739442924665	33.0	33.0	33.0	33.0	33.0
4	36.62517637526399	37.0	37.0	37.0	37.0	37.0
5	36.62825306689379	37.0	37.0	37.0	37.0	37.0
6	36.20052731022654	37.0	37.0	37.0	33.0	37.0
7	36.06875667707365	37.0	37.0	37.0	33.0	37.0
8	35.96844519042317	37.0	37.0	37.0	33.0	37.0
9	35.97241616803089	37.0	37.0	37.0	33.0	37.0
10-11	35.89475779905356	37.0	37.0	37.0	33.0	37.0
12-13	35.807908151250665	37.0	37.0	37.0	33.0	37.0
14-15	37.564120801568464	40.0	37.0	40.0	33.0	40.0
16-17	37.49625948343521	40.0	37.0	40.0	33.0	40.0
18-19	37.51698764997615	40.0	37.0	40.0	33.0	40.0
20-21	37.434002583021396	40.0	37.0	40.0	33.0	40.0
22-23	37.308945892918956	40.0	37.0	40.0	33.0	40.0
24-25	37.29137234029606	40.0	37.0	40.0	33.0	40.0
26-27	37.140191917243456	40.0	37.0	40.0	33.0	40.0
28-29	36.976740910685606	40.0	37.0	40.0	33.0	40.0
30-31	36.799048343466914	40.0	37.0	40.0	33.0	40.0
32-33	36.59261312329892	40.0	37.0	40.0	33.0	40.0
34-35	36.50263087776211	40.0	37.0	40.0	33.0	40.0
36-37	36.30140277180815	40.0	37.0	40.0	30.0	40.0
38-39	36.33405816000483	40.0	37.0	40.0	30.0	40.0
40-41	36.37398404938946	40.0	37.0	40.0	33.0	40.0
42-43	36.29007361468443	40.0	37.0	40.0	33.0	40.0
44-45	36.15869933588047	38.5	37.0	40.0	27.0	40.0
46-47	35.989006031075135	37.0	37.0	40.0	27.0	40.0
48-49	35.79129019281487	37.0	37.0	40.0	27.0	40.0
50-51	35.774462920751034	37.0	37.0	40.0	27.0	40.0
52-53	35.650894154567666	37.0	37.0	40.0	27.0	40.0
54-55	35.46225603119598	37.0	33.0	40.0	27.0	40.0
56-57	35.23084406039968	37.0	33.0	40.0	27.0	40.0
58-59	35.09890519386882	37.0	33.0	40.0	27.0	40.0
60-61	34.96581456989011	37.0	33.0	40.0	27.0	40.0
62-63	34.79597719641849	37.0	33.0	40.0	27.0	40.0
64-65	34.61022949762341	37.0	33.0	40.0	27.0	40.0
66-67	34.35126074669733	37.0	33.0	37.0	27.0	40.0
68-69	34.14027144118012	37.0	33.0	37.0	27.0	40.0
70-71	33.90858636735502	37.0	33.0	37.0	27.0	40.0
72-73	33.61762149244514	37.0	33.0	37.0	27.0	40.0
74-75	33.28344141976066	37.0	33.0	37.0	24.5	38.5
76-77	31.16653920631803	33.0	30.0	35.0	24.5	37.0
78-79	33.27911532364068	35.0	33.0	37.0	27.0	37.0
80-81	33.647148051061464	37.0	33.0	37.0	27.0	37.0
82-83	33.609491107357854	37.0	33.0	37.0	27.0	37.0
84-85	33.5241283090657	37.0	33.0	37.0	27.0	37.0
86-87	33.394450127299535	37.0	33.0	37.0	27.0	37.0
88-89	33.24720538010837	37.0	33.0	37.0	27.0	37.0
90-91	33.1732916301878	33.0	33.0	37.0	27.0	37.0
92-93	33.218675117888495	33.0	33.0	37.0	27.0	37.0
94-95	33.27640329186457	33.0	33.0	37.0	27.0	37.0
96-97	33.389865665157274	33.0	33.0	37.0	27.0	37.0
98-99	33.533004109422336	33.0	33.0	37.0	27.0	37.0
100	33.5058499661098	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	5.0
15	17.0
16	33.0
17	119.0
18	375.0
19	1043.0
20	2587.0
21	6850.0
22	16744.0
23	37102.0
24	70722.0
25	117340.0
26	170191.0
27	223775.0
28	269492.0
29	304344.0
30	327666.0
31	346231.0
32	370192.0
33	413629.0
34	492908.0
35	651062.0
36	1059123.0
37	2230805.0
38	1721889.0
39	19746.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.00526271147102	19.017841784569242	12.589565541686229	24.387329962273512
2	15.569738301013864	20.94126113867131	40.116316433194626	23.3726841271202
3	18.87967126012022	26.099431796041582	29.383261459321975	25.637635484516224
4	12.726370375457213	17.105093141436193	38.00191830877995	32.16661817432665
5	13.607177510611319	38.125403137501074	34.84996423645884	13.417455115428764
6	31.34584166620454	36.773055224474476	18.43120238093759	13.449900728383394
7	27.827326682396674	31.939460973023355	22.509351997308332	17.723860347271643
8	25.956870749021544	35.50751293964496	20.76308864556108	17.77252766577242
9	26.980126815127775	15.630510579918141	20.03557491757107	37.35378768738301
10-11	25.31159855163449	25.957793785518273	28.35145729087612	20.379150371971118
12-13	26.237972326850244	23.461960572419713	28.223559844510632	22.07650725621941
14-15	23.242801650293842	24.6564767979384	25.764290980851385	26.336430570916374
16-17	22.965937055955894	27.57389294838904	26.37744944624407	23.082720549410997
18-19	22.967026959932532	26.882442053532696	27.941840013164686	22.20869097337009
20-21	23.923456546542912	26.156932566597025	27.604484659341246	22.31512622751882
22-23	23.531513982602974	26.11933090874875	27.54122785251909	22.807927256129183
24-25	23.241051086823596	26.2644034133372	27.691717131456056	22.80282836838315
26-27	23.229337959888436	26.428747863071848	27.6382772776531	22.70363689938661
28-29	23.120540345130745	26.45654635452768	27.631968239620434	22.790945060721132
30-31	23.418630560599958	26.35798905843566	27.461555246956692	22.761825134007687
32-33	23.136390882192778	26.5878063221452	27.478450589528798	22.797352206133226
34-35	23.28148024030711	26.584728720424692	27.45614234256036	22.677648696707834
36-37	23.330347293813876	26.504263839253884	27.367782587068838	22.797606279863402
38-39	23.138670436582178	26.69972322489258	27.513761175744044	22.6478451627812
40-41	23.350706452234007	26.532960746591172	27.416912824227442	22.699419976947375
42-43	23.193862451058912	26.56656321811183	27.30989671911085	22.929677611718404
44-45	23.26105570673295	26.71552624542362	27.098828885065355	22.924589162778076
46-47	23.37845766477372	26.610880395184317	26.811060478061027	23.19960146198094
48-49	23.18653241217375	26.742902565954367	26.921494648215837	23.149070373656045
50-51	23.171558367964764	26.902159776606617	26.807096830183376	23.119185025245248
52-53	23.36950132127612	26.833033306901704	26.645826580449512	23.15163879137267
54-55	23.316491358077542	26.6948506192695	26.710522722458368	23.278135300194585
56-57	23.30263606117612	26.936964745824625	26.631960248236624	23.12843894476263
58-59	23.23099236553473	26.963623900816675	26.72568877240567	23.079694961242925
60-61	23.285727562264817	27.00553869946046	26.69183290562244	23.016900832652286
62-63	23.25869012915361	27.164995511600093	26.590390123639985	22.985924235606312
64-65	23.216792274585057	27.042472042676184	26.621267519843993	23.11946816289476
66-67	23.297305474777698	27.038918621174762	26.536198752752515	23.127577151295025
68-69	23.217839667998806	27.018019068496812	26.610978169894373	23.15316309361001
70-71	23.228049899747038	26.685281391122125	26.74564048528159	23.341028223849246
72-73	23.25756431749897	26.773327508826334	26.640949132786286	23.328159040888412
74-75	23.004860604004534	26.97926620826237	26.6794534383597	23.336419749373395
76-77	23.161630289769565	26.889613207438035	26.676669203619962	23.272087299172437
78-79	23.054036104619495	26.908707378334544	26.650546331841586	23.38671018520437
80-81	23.096591871563547	26.943744714955812	26.64208440260478	23.317579010875853
82-83	23.1083707725952	26.83551796284415	26.81413201634144	23.241979248219206
84-85	23.200152168499493	26.849856291551923	26.717748794372408	23.23224274557618
86-87	22.959744344828593	26.906393180317973	26.829241796653108	23.304620678200326
88-89	23.04458965943361	26.844886696053678	26.812184547932688	23.29833909658003
90-91	23.055198214114327	26.85781498844507	26.86273673234477	23.224250065095834
92-93	23.11597858443921	26.81889087039895	26.873760523515212	23.19137002164663
94-95	22.928470277145955	26.882483974660225	26.929048317261	23.25999743093282
96-97	22.939820672912944	26.921995739707423	27.0889748337725	23.049208753607136
98-99	24.07424251408613	28.90859905341483	27.228763209982475	19.78839522251656
100	23.141488331978923	29.762170124464827	20.900530596158884	26.19581094739737
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	970.0
1	952.5
2	1532.0
3	2375.0
4	3577.5
5	6572.5
6	9077.0
7	9988.0
8	9910.0
9	8901.5
10	7563.0
11	6516.5
12	7009.5
13	9995.5
14	16850.0
15	26509.0
16	35253.0
17	40888.0
18	42563.0
19	40078.5
20	36019.0
21	31872.5
22	28939.0
23	28870.0
24	31489.0
25	37343.0
26	46533.5
27	57525.0
28	68616.5
29	82068.0
30	96710.5
31	112453.5
32	132381.5
33	152742.0
34	173515.0
35	195414.5
36	218757.0
37	241207.0
38	259369.0
39	274871.0
40	292719.0
41	309761.5
42	322676.5
43	336907.0
44	351162.5
45	365237.0
46	376778.0
47	385624.0
48	395145.5
49	398638.5
50	397764.5
51	396061.5
52	388885.5
53	377420.0
54	363662.0
55	344946.5
56	322947.5
57	298678.0
58	271736.0
59	242975.5
60	209665.5
61	175416.0
62	143678.5
63	115050.0
64	89846.0
65	69049.0
66	53311.5
67	41810.0
68	32809.5
69	24884.0
70	18535.5
71	13832.5
72	10209.0
73	7306.0
74	5308.0
75	3947.5
76	2943.0
77	2172.0
78	1639.0
79	1246.5
80	886.5
81	622.0
82	482.0
83	341.0
84	226.0
85	166.5
86	126.0
87	98.5
88	70.0
89	48.0
90	33.0
91	25.5
92	21.5
93	13.5
94	4.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.517736690719473E-4
3	0.0034334798849467996
4	0.004054668679920727
5	2.2588683453597368E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.1859058813138616E-4
12-13	0.001558619158298218
14-15	0.0033318308094056116
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.006635094486689389
26-27	0.020600581830309515
28-29	0.009043995625891423
30-31	0.0
32-33	0.0
34-35	1.2645911545182664E-4
36-37	0.01315108629531532
38-39	0.03566520329745707
40-41	0.040782763347745686
42-43	0.007520940367266922
44-45	3.0700485297921346E-4
46-47	5.954727398534422E-6
48-49	0.03487829714627418
50-51	0.056762074121778194
52-53	0.05335973540013944
54-55	0.03012343228564171
56-57	0.0013444874990233878
58-59	0.006609219348745767
60-61	0.030072158683677762
62-63	0.035133868420519576
64-65	0.01579155556164126
66-67	0.04199905145087368
68-69	0.05786351696451629
70-71	0.07201571323671828
72-73	0.049955300185729204
74-75	0.021916903130896434
76-77	0.00503060869827216
78-79	4.402223136229098E-4
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0024645341620918907
92-93	0.00266905712506706
94-95	5.169437720259167E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2524.0
20-21	7459.0
22-23	8632.0
24-25	15327.0
26-27	20761.0
28-29	29366.0
30-31	29521.0
32-33	32432.0
34-35	37361.0
36-37	37664.0
38-39	40613.0
40-41	49198.0
42-43	57332.0
44-45	68190.0
46-47	78550.0
48-49	58712.0
50-51	69883.0
52-53	63376.0
54-55	61305.0
56-57	62807.0
58-59	74001.0
60-61	61175.0
62-63	57435.0
64-65	68462.0
66-67	79610.0
68-69	60752.0
70-71	67800.0
72-73	57880.0
74-75	51766.0
76-77	42944.0
78-79	72199.0
80-81	90946.0
82-83	93821.0
84-85	117107.0
86-87	128592.0
88-89	143841.0
90-91	157031.0
92-93	168838.0
94-95	191805.0
96-97	379134.0
98-99	577552.0
100-101	5280287.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.28707553336584
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.62652208050616	77.29271034006376
2	6.2546052445327405	10.668739798436999
3	1.6750293228658386	4.285750571395843
4	0.635750144354591	2.1688508232767294
5	0.30522258691766024	1.3015770912467906
6	0.16734540593153124	0.8563440165506564
7	0.08868816806970897	0.529476814235398
8	0.06155912810399644	0.42001584066989506
9	0.042409046439823225	0.3255249192310101
>10	0.1386625276694924	1.837376933270021
>50	0.003350824714168556	0.193865714164824
>100	8.103761704445337E-4	0.09693560660182783
>500	4.514372389740841E-5	0.022831530856222207
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1294341726798684E-5
2	0.0	4.5177366907194735E-5	0.0	0.0	1.1294341726798684E-5
3	0.0	4.5177366907194735E-5	0.0	0.0	1.1294341726798684E-5
4	0.0	1.0164907554118815E-4	0.0	0.0	1.1294341726798684E-5
5	0.0	1.1294341726798684E-4	0.0	0.0	1.1294341726798684E-5
6	0.0	1.1294341726798684E-4	0.0	0.0	4.5177366907194735E-5
7	0.0	1.1294341726798684E-4	0.0	1.1294341726798684E-5	5.647170863399342E-5
8	0.0	1.355321007215842E-4	0.0	1.1294341726798684E-5	5.647170863399342E-5
9	0.0	2.2588683453597368E-4	0.0	1.1294341726798684E-5	9.035473381438947E-5
10-11	0.0	2.2588683453597368E-4	0.0	1.1294341726798684E-5	9.035473381438947E-5
12-13	0.0	2.2588683453597368E-4	0.0	1.6941512590198026E-5	9.035473381438947E-5
14-15	0.0	3.275359100771618E-4	0.0	2.823585431699671E-5	1.4682644244838288E-4
16-17	0.0	3.275359100771618E-4	0.0	4.5177366907194735E-5	1.6941512590198024E-4
18-19	0.0	4.0094913130135325E-4	0.0	4.5177366907194735E-5	1.9200380935557762E-4
20-21	0.0	4.743623525255447E-4	0.0	4.5177366907194735E-5	1.9200380935557762E-4
22-23	0.0	5.251868902961389E-4	0.0	7.341322122419144E-5	2.0894532194577563E-4
24-25	0.0	5.873057697935315E-4	0.0	1.1294341726798682E-4	2.48475517989571E-4
26-27	5.647170863399342E-6	6.211887949739276E-4	0.0	2.2588683453597368E-4	2.48475517989571E-4
28-29	1.1294341726798684E-5	6.211887949739276E-4	0.0	4.6306801079874603E-4	2.541226888529704E-4
30-31	1.1294341726798684E-5	6.77660503607921E-4	0.0	0.0012932021277184491	2.5976985971636974E-4
32-33	1.1294341726798684E-5	7.115435287883171E-4	0.0	0.00317371002523043	2.5976985971636974E-4
34-35	1.1294341726798684E-5	7.962510917393071E-4	0.0	0.005986001115203302	2.710642014431684E-4
36-37	1.1294341726798684E-5	8.809586546902972E-4	0.0	0.009972903744763237	2.710642014431684E-4
38-39	1.1294341726798684E-5	9.204888507340927E-4	0.0	0.017184340937324197	2.8800571403336643E-4
40-41	1.1294341726798684E-5	0.0010051964136850828	0.0	0.024920965020181297	3.0494722662356444E-4
42-43	1.1294341726798684E-5	0.001039079438865479	0.0	0.03217193240878605	3.105943974869638E-4
44-45	1.1294341726798684E-5	0.0011520228561334657	0.0	0.04056362831179747	3.2188873921376246E-4
46-47	1.1294341726798684E-5	0.0012480247608112546	0.0	0.048989207239989285	3.3318308094056114E-4
48-49	1.1294341726798684E-5	0.001253671931674654	0.0	0.055777106617795294	3.501245935307592E-4
50-51	1.1294341726798684E-5	0.0014795587662106277	0.0	0.061825226612495995	3.501245935307592E-4
52-53	1.1294341726798684E-5	0.0014908531079374262	0.0	0.0678338164111529	3.783604478477559E-4
54-55	1.1294341726798684E-5	0.0015077946205276243	0.0	0.07275250223317373	4.065963021647526E-4
56-57	1.1294341726798684E-5	0.0015247361331178222	0.0	0.07685234828000165	4.065963021647526E-4
58-59	1.1294341726798684E-5	0.0015303833039812214	0.0	0.08039312441135303	4.065963021647526E-4
60-61	1.1294341726798684E-5	0.0015642663291616176	0.0	0.08367977785385144	4.065963021647526E-4
62-63	1.1294341726798684E-5	0.0015812078417518157	0.0	0.08795468619744475	4.1789064389155127E-4
64-65	1.1294341726798684E-5	0.001750622967653796	0.0	0.09147852081620593	4.1789064389155127E-4
66-67	1.1294341726798684E-5	0.0017845059928341918	0.0	0.09575342915979923	4.235378147549506E-4
68-69	1.1294341726798684E-5	0.0018409777014681853	0.0	0.09977421481453957	4.2918498561834995E-4
70-71	1.1294341726798684E-5	0.0018805078975119808	0.0	0.10248485682897125	4.404793273451486E-4
72-73	1.1294341726798684E-5	0.0018918022392387795	0.0	0.10620069525708803	4.96951035979142E-4
74-75	1.1294341726798684E-5	0.0019369796061459743	0.0	0.10912028259346548	4.96951035979142E-4
76-77	1.1294341726798684E-5	0.001999098485643367	0.0	0.1114582113309128	4.96951035979142E-4
78-79	1.1294341726798684E-5	0.002032981510823763	0.0	0.11301118331834763	5.025982068425414E-4
80-81	1.1294341726798684E-5	0.002032981510823763	0.0	0.11359284191727775	5.195397194327395E-4
82-83	1.1294341726798684E-5	0.0020386286816871622	0.0	0.11517404975902956	5.534227446131355E-4
84-85	1.1294341726798684E-5	0.0020442758525505617	0.0	0.11643336886156763	5.534227446131355E-4
86-87	1.1294341726798684E-5	0.002078158877730958	0.0	0.11730303317453113	5.647170863399342E-4
88	1.1294341726798684E-5	0.0022588683453597367	0.0	0.11738209356661872	5.647170863399342E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10005	0.0	44.90518	1
GTATCAA	17600	0.0	39.071056	1
TCAACGC	21785	0.0	30.730219	4
ATCAACG	22125	0.0	30.277935	3
CAACGCA	22250	0.0	29.954351	5
TATCAAC	23110	0.0	29.42635	2
AACGCAG	23025	0.0	29.002325	6
GTACATG	30425	0.0	27.296833	1
TACATGG	29795	0.0	27.235672	2
ACATGGG	30625	0.0	26.09731	3
ACGCAGA	26940	0.0	24.662115	7
CGCAGAG	27675	0.0	24.02322	8
CATGGGG	23490	0.0	23.29994	4
GCAGAGT	32915	0.0	20.033085	9
ATGGGGG	13455	0.0	19.631546	5
AGAGTAC	28415	0.0	18.408339	10-11
GAGTACT	17295	0.0	18.060608	12-13
CAGAGTA	31005	0.0	17.59314	10-11
GTGGTAT	5225	0.0	17.553097	1
GTATAAT	5240	0.0	17.50285	1
>>END_MODULE