FastQCFastQC Report
Fri 27 May 2016
SRR2139353_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139353_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8541151
Sequences flagged as poor quality0
Sequence length19-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96250.045.742851
GTATCAA166200.036.79261
TCAACGC197850.029.9899984
ATCAACG201350.029.5109253
CAACGCA204150.028.980535
TATCAAC210850.028.541312
AACGCAG212000.028.0474916
GTACATG282350.027.3030951
TACATGG281950.026.8183292
ACATGGG289600.025.5856633
ACGCAGA248800.023.763397
CATGGGG223400.023.6152484
CGCAGAG253450.023.3608238
GTGGTAT47000.020.9065111
GCAGAGT306450.019.3902079
ATGGGGG137050.019.2308735
TGGTATC48950.018.5229112
GAGTACT161800.018.32321212-13
CAGAGTA293100.017.28777310-11
AGAGTAC265100.017.15700710-11
Produced by FastQC (version 0.11.5)