FastQCFastQC Report
Fri 27 May 2016
SRR2139352_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139352_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9133554
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97950.049.2711141
GTATCAA182500.038.624311
TCAACGC215200.032.0382584
ATCAACG219500.031.5093733
CAACGCA221550.031.0609155
TATCAAC228550.030.4866222
AACGCAG226550.030.3007566
ACGCAGA264500.025.8078167
CGCAGAG269000.025.361318
GTACATG260200.024.544051
TACATGG259800.024.186952
CTCGTAT24850.023.738769
ACATGGG261550.023.4036773
AATCTCG25450.023.1773266
TCTCGTA28150.020.9551948
GCAGAGT325600.020.9271079
CATGGGG197150.020.9221974
GAGTACT194450.019.07044812-13
CAGAGTA312650.018.37579510-11
AGAGTAC288800.018.30955710-11
Produced by FastQC (version 0.11.5)