##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139352_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9133554
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.65807187432187	33.0	33.0	33.0	33.0	33.0
2	32.65189760743737	33.0	33.0	33.0	33.0	33.0
3	32.7314059784395	33.0	33.0	33.0	33.0	33.0
4	36.67470833368916	37.0	37.0	37.0	37.0	37.0
5	36.69136636187841	37.0	37.0	37.0	37.0	37.0
6	36.3391825350789	37.0	37.0	37.0	37.0	37.0
7	36.2304722783705	37.0	37.0	37.0	37.0	37.0
8	36.14563553245539	37.0	37.0	37.0	33.0	37.0
9	36.14321730620961	37.0	37.0	37.0	33.0	37.0
10-11	36.079409066832035	37.0	37.0	37.0	33.0	37.0
12-13	35.99026600160244	37.0	37.0	37.0	33.0	37.0
14-15	37.82164385298428	40.0	37.0	40.0	33.0	40.0
16-17	37.76342226695107	40.0	37.0	40.0	33.0	40.0
18-19	37.79346889502159	40.0	37.0	40.0	33.0	40.0
20-21	37.70945786486711	40.0	37.0	40.0	33.0	40.0
22-23	37.57633786986162	40.0	37.0	40.0	33.0	40.0
24-25	37.574236845040346	40.0	37.0	40.0	33.0	40.0
26-27	37.451067854178916	40.0	37.0	40.0	33.0	40.0
28-29	37.284397356397506	40.0	37.0	40.0	33.0	40.0
30-31	37.111390821741495	40.0	37.0	40.0	33.0	40.0
32-33	36.91832057946746	40.0	37.0	40.0	33.0	40.0
34-35	36.832260950434176	40.0	37.0	40.0	33.0	40.0
36-37	36.60456323196112	40.0	37.0	40.0	33.0	40.0
38-39	36.66170180603692	40.0	37.0	40.0	33.0	40.0
40-41	36.706240382822216	40.0	37.0	40.0	33.0	40.0
42-43	36.61727733125668	40.0	37.0	40.0	33.0	40.0
44-45	36.47286277741537	40.0	37.0	40.0	33.0	40.0
46-47	36.27122365518913	38.5	37.0	40.0	30.0	40.0
48-49	36.070662230219334	37.0	37.0	40.0	27.0	40.0
50-51	36.08326022718846	37.0	37.0	40.0	27.0	40.0
52-53	35.94910064018147	37.0	37.0	40.0	27.0	40.0
54-55	35.756961437251704	37.0	37.0	40.0	27.0	40.0
56-57	35.5086639429607	37.0	35.0	40.0	27.0	40.0
58-59	35.36908368923412	37.0	33.0	40.0	27.0	40.0
60-61	35.23854614719704	37.0	33.0	40.0	27.0	40.0
62-63	35.05640248002367	37.0	33.0	40.0	27.0	40.0
64-65	34.91202853031868	37.0	33.0	40.0	27.0	40.0
66-67	34.66459536006531	37.0	33.0	38.5	27.0	40.0
68-69	34.47785551129243	37.0	33.0	37.0	27.0	40.0
70-71	34.24036163978842	37.0	33.0	37.0	27.0	40.0
72-73	33.938357845481704	37.0	33.0	37.0	27.0	40.0
74-75	33.57925473399166	37.0	33.0	37.0	27.0	40.0
76-77	31.540493993676318	33.0	30.0	35.0	24.5	37.0
78-79	33.58425800683065	37.0	33.0	37.0	27.0	37.0
80-81	33.91665228179082	37.0	33.0	37.0	27.0	37.0
82-83	33.85483883878068	37.0	33.0	37.0	27.0	37.0
84-85	33.741990400234286	37.0	33.0	37.0	27.0	37.0
86-87	33.60237678387441	37.0	33.0	37.0	27.0	37.0
88-89	33.43664731821575	37.0	33.0	37.0	27.0	37.0
90-91	33.349090937133795	37.0	33.0	37.0	27.0	37.0
92-93	33.378569689086675	37.0	33.0	37.0	27.0	37.0
94-95	33.414420507985625	35.0	33.0	37.0	27.0	37.0
96-97	33.51519029302473	33.0	33.0	37.0	27.0	37.0
98-99	33.65394916099251	33.0	33.0	37.0	27.0	37.0
100	33.60829581440623	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	10.0
15	20.0
16	50.0
17	181.0
18	544.0
19	1384.0
20	3266.0
21	6936.0
22	14721.0
23	29723.0
24	55068.0
25	90003.0
26	134662.0
27	182521.0
28	228762.0
29	271582.0
30	308460.0
31	343265.0
32	386658.0
33	444598.0
34	537208.0
35	692909.0
36	1062852.0
37	2296995.0
38	2022346.0
39	18827.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.28312943679974	19.492532698662536	12.836470885265472	24.387866979272253
2	15.147506593864996	21.25637625923935	40.60813555387919	22.98798159301647
3	18.372103743141377	26.480150299823357	30.033632202299938	25.114113754735328
4	12.58056735226449	17.52819959976775	38.82797009634195	31.06326295162581
5	13.22037546615211	38.19258507614725	35.485040706502716	13.101998751197918
6	30.40221801940406	37.74273409890608	18.740755241606934	13.114292640082931
7	26.853676017024696	32.47596718648623	23.286192866435123	17.38416393005395
8	25.064602453765534	36.09971540103666	21.395439278072917	17.440242867124887
9	26.48190397735646	15.887211046214869	20.695021893996575	36.9358630824321
10-11	24.564751204639645	26.31193237715429	29.14040836628469	19.982908051921378
12-13	25.559234981173436	23.922861988510267	28.966109990069384	21.55179304024692
14-15	22.52560969467638	25.204390327221603	26.512805942237495	25.757194035864522
16-17	22.25468311678017	28.117592560354925	27.123280817083906	22.50444350578099
18-19	22.32597518994249	27.367670897878305	28.69933215482166	21.607021757357543
20-21	23.056219918836575	26.69008688696196	28.489842086829135	21.763851107372325
22-23	22.728731632647804	26.65844343453282	28.409298239020025	22.203526693799354
24-25	22.488549368830636	26.74496410223564	28.525227142662544	22.241259386271174
26-27	22.496564715940824	26.96611627249616	28.359802818731506	22.17751619283151
28-29	22.413358637318943	27.053437917954188	28.428502718226234	22.104700726500635
30-31	22.749186225772558	26.942173144619876	28.233996095473202	22.074644534134364
32-33	22.429167677842127	27.16615644390183	28.243980473636892	22.16069540461915
34-35	22.579603302243903	27.17480198403855	28.22245359478569	22.02314111893186
36-37	22.623487456384215	27.118074025008056	28.151359742001958	22.107078776605768
38-39	22.420429338155863	27.243731177400342	28.260615069596078	22.075224414847717
40-41	22.64827972172175	27.0873021380429	28.142841681900293	22.121576458335056
42-43	22.52498010844264	27.12047281841593	28.01843212674853	22.336114946392904
44-45	22.575120358869015	27.257560410494396	27.83273449363339	22.334584737003194
46-47	22.649453445553643	27.214254894193036	27.570972479934742	22.56531918031858
48-49	22.561983418557823	27.272889023732745	27.6198160463583	22.545311511351137
50-51	22.544498101881953	27.414307189381816	27.546907833980068	22.494286874756174
52-53	22.709144407408537	27.34410567173276	27.42314172309468	22.523608197764023
54-55	22.628840215614698	27.255185638906955	27.468914924123837	22.64705922135451
56-57	22.603191143042675	27.400741255679712	27.428791774782972	22.567275826494637
58-59	22.602239761207883	27.42948793354898	27.43295195509436	22.535320350148783
60-61	22.62921824109126	27.44914535240792	27.44003351618072	22.4816028903201
62-63	22.582734063913325	27.573376156558695	27.370499070859584	22.473390708668394
64-65	22.579653166092914	27.482096964711978	27.383659400918166	22.55459046827695
66-67	22.632210241545327	27.48117428883477	27.3260339548399	22.560581514780008
68-69	22.541676447762693	27.51222974994224	27.388936854358647	22.557156947936416
70-71	22.589261089259498	27.290136354192235	27.434839450089537	22.685763106458726
72-73	22.592933804807096	27.336514921792414	27.344620866298452	22.72593040710204
74-75	22.48558191159517	27.446768283875272	27.306296778016044	22.761353026513515
76-77	22.581909411186103	27.365050326055684	27.330279132755024	22.72276113000318
78-79	22.490332761592143	27.353191941917192	27.360127846707254	22.796347449783408
80-81	22.45262735437298	27.411001856042745	27.39962682107504	22.736743968509238
82-83	22.50645599604744	27.38387535426413	27.453126330443723	22.65654231924471
84-85	22.597031370453593	27.334547426512103	27.414104189456882	22.654317013577426
86-87	22.416029754831353	27.36786621110535	27.496837066280396	22.7192669677829
88-89	22.432773879926337	27.33759780843456	27.48574355561193	22.74388475602717
90-91	22.471917046931818	27.317295990762545	27.545111425684322	22.665675536621315
92-93	22.438600711712713	27.292217608631496	27.598790616599512	22.67039106305628
94-95	22.31926625400772	27.324072128784476	27.67369878214831	22.682962835059488
96-97	22.329207994527348	27.42724177256572	27.811916670812497	22.431633562094433
98-99	23.414410944931248	29.326864808320313	28.103220271145517	19.155503975602922
100	22.445026486514102	30.197705801647633	21.812330181274675	25.544937530563587
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1289.0
1	1229.0
2	2056.5
3	3135.5
4	4237.5
5	7103.0
6	9687.0
7	11328.0
8	12377.0
9	12054.0
10	10750.5
11	9449.5
12	9874.5
13	12693.0
14	19477.0
15	29023.0
16	37429.0
17	43069.5
18	44489.5
19	42128.0
20	38300.5
21	34274.0
22	31891.0
23	32935.5
24	37189.0
25	44832.5
26	56232.0
27	69731.5
28	84181.5
29	101175.5
30	119388.0
31	138253.5
32	160147.5
33	182019.0
34	204765.5
35	227443.5
36	250714.0
37	275416.0
38	296000.5
39	313822.5
40	333076.5
41	349822.0
42	360297.5
43	369223.5
44	377634.5
45	385957.5
46	392585.0
47	396651.5
48	398546.0
49	394702.5
50	388433.5
51	379109.5
52	364170.5
53	346081.0
54	325961.0
55	303523.5
56	279204.0
57	252836.0
58	225440.5
59	198336.5
60	169181.0
61	140862.0
62	115311.5
63	91809.5
64	71186.0
65	54805.0
66	42532.0
67	33628.5
68	26814.0
69	20473.5
70	15475.5
71	11937.5
72	9054.5
73	6683.0
74	4915.0
75	3667.0
76	2715.5
77	1945.5
78	1381.5
79	1032.0
80	772.0
81	553.5
82	421.0
83	308.0
84	210.0
85	149.0
86	114.0
87	91.5
88	62.5
89	39.0
90	27.5
91	20.0
92	14.0
93	7.5
94	3.5
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	7.007129973721072E-4
3	0.0037553837202911377
4	0.003908664688466286
5	1.0948640583939176E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.4780664788317888E-4
12-13	0.0020145498674448083
14-15	0.0032189003316781175
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.006157878912850879
26-27	0.019190302127284797
28-29	0.008437497162045353
30-31	0.0
32-33	1.1068738136734005E-5
34-35	1.6104363382853695E-4
36-37	0.016395397447903918
38-39	0.043654574903708825
40-41	0.04997990678367684
42-43	0.00894672401166128
44-45	3.3577555987586774E-4
46-47	0.0
48-49	0.04275907292093608
50-51	0.053930533987214195
52-53	0.05779498290460637
54-55	0.03589584139093858
56-57	0.0014971888356344672
58-59	0.006081044947686022
60-61	0.02850465207691248
62-63	0.0416620058279671
64-65	0.02225271849076199
66-67	0.05529599984295789
68-69	0.0699043837727774
70-71	0.07324341016677657
72-73	0.04675238733856347
74-75	0.021386542459542364
76-77	0.0052048829456269605
78-79	5.45878415665606E-4
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.002328386027327397
92-93	0.002806065090806347
94-95	7.661053766159298E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1519.0
20-21	5122.0
22-23	5874.0
24-25	11209.0
26-27	16599.0
28-29	26899.0
30-31	25351.0
32-33	28310.0
34-35	34173.0
36-37	38396.0
38-39	40534.0
40-41	45875.0
42-43	52414.0
44-45	63742.0
46-47	65861.0
48-49	50539.0
50-51	52421.0
52-53	50179.0
54-55	54808.0
56-57	58554.0
58-59	65332.0
60-61	52050.0
62-63	55541.0
64-65	55588.0
66-67	81228.0
68-69	59008.0
70-71	67207.0
72-73	64080.0
74-75	55877.0
76-77	45240.0
78-79	71850.0
80-81	72743.0
82-83	89592.0
84-85	119782.0
86-87	132976.0
88-89	148051.0
90-91	159948.0
92-93	173986.0
94-95	201254.0
96-97	400426.0
98-99	616029.0
100-101	5617387.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.03176049039631
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.82037370852485	78.13436638540095
2	6.281001675732857	10.807312636128541
3	1.5830943352044664	4.085891780400415
4	0.5999629273285452	2.064634674681858
5	0.2644649051446248	1.137619068875887
6	0.1437307665794165	0.741924653127685
7	0.08934300041071881	0.5380434928979837
8	0.056460181744868554	0.38858950664950215
9	0.03920705854005549	0.3035747042865901
>10	0.11975505531281819	1.5682389981599802
>50	0.0022085198412106666	0.12853638338743317
>100	3.162097714716046E-4	0.04619215432200723
>500	6.124241821611238E-5	0.03540606918508144
>1k	2.041344588487342E-5	0.01966949249609227
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0948640583939176E-5
2	0.0	7.664048408757424E-5	0.0	0.0	1.0948640583939176E-5
3	0.0	7.664048408757424E-5	0.0	0.0	1.0948640583939176E-5
4	0.0	9.853776525545259E-5	0.0	1.0948640583939176E-5	1.0948640583939176E-5
5	0.0	1.2043504642333094E-4	0.0	1.0948640583939176E-5	1.0948640583939176E-5
6	0.0	1.2043504642333094E-4	0.0	1.0948640583939176E-5	2.189728116787835E-5
7	0.0	1.2043504642333094E-4	0.0	1.0948640583939176E-5	2.189728116787835E-5
8	0.0	1.313836870072701E-4	0.0	1.0948640583939176E-5	2.189728116787835E-5
9	0.0	2.1897281167878352E-4	0.0	2.189728116787835E-5	3.284592175181753E-5
10-11	0.0	2.1897281167878352E-4	0.0	2.737160145984794E-5	3.832024204378711E-5
12-13	1.6422960875908764E-5	2.1897281167878352E-4	0.0	4.37945623357567E-5	4.9268882627726294E-5
14-15	2.189728116787835E-5	3.667794595619624E-4	0.0	4.37945623357567E-5	5.474320291969588E-5
16-17	2.737160145984794E-5	3.72253779853932E-4	0.0	6.569184350363505E-5	5.474320291969588E-5
18-19	3.284592175181753E-5	4.269969827736279E-4	0.0	8.75891246715134E-5	7.664048408757424E-5
20-21	3.284592175181753E-5	4.926888262772629E-4	0.0	1.313836870072701E-4	7.664048408757424E-5
22-23	4.37945623357567E-5	4.981631465692325E-4	0.0	1.5328096817514847E-4	1.0948640583939176E-4
24-25	5.474320291969588E-5	6.076495524086243E-4	0.0	2.0802417109484436E-4	1.368580072992397E-4
26-27	6.569184350363505E-5	6.569184350363506E-4	0.0	2.846646551824186E-4	1.5328096817514847E-4
28-29	6.569184350363505E-5	6.569184350363506E-4	0.0	7.992507626275599E-4	1.5328096817514847E-4
30-31	6.569184350363505E-5	6.897643567881681E-4	0.0	0.0022773172414593487	1.5328096817514847E-4
32-33	6.569184350363505E-5	7.007129973721072E-4	0.0	0.005611178299268828	1.5328096817514847E-4
34-35	6.569184350363505E-5	8.101994032114991E-4	0.0	0.01077346233459615	1.5328096817514847E-4
36-37	6.569184350363505E-5	9.361087699267996E-4	0.0	0.017983142159120096	1.5328096817514847E-4
38-39	6.569184350363505E-5	9.799033322625563E-4	0.0	0.029725559185394865	1.6422960875908763E-4
40-41	6.569184350363505E-5	0.0010839154178099784	0.0	0.041489873492837503	1.9707553051090518E-4
42-43	6.569184350363505E-5	0.0011277099801457352	0.0	0.05175422404028049	2.0254985080287477E-4
44-45	6.569184350363505E-5	0.0012536193468610356	0.0	0.06378678004202964	2.1349849138681395E-4
46-47	6.569184350363505E-5	0.0013466827918245186	0.0	0.07555109434947228	2.572930537225706E-4
48-49	6.569184350363505E-5	0.001352157112116488	0.0	0.08412935424698864	2.737160145984794E-4
50-51	6.569184350363505E-5	0.0015656556035033022	0.0	0.09143209751647606	2.79190334890449E-4
52-53	6.569184350363505E-5	0.0015875528846711806	0.0	0.09944650242391953	3.2298489722620567E-4
54-55	6.569184350363505E-5	0.001609450165839059	0.0	0.1061306475004144	3.613051392699928E-4
56-57	6.569184350363505E-5	0.0016532447281748157	0.0	0.11149548138654461	3.613051392699928E-4
58-59	6.569184350363505E-5	0.0016587190484667852	0.0	0.11595157810420785	3.667794595619624E-4
60-61	6.569184350363505E-5	0.001713462251386481	0.0	0.11972338478537489	3.72253779853932E-4
62-63	6.569184350363505E-5	0.0017517824934302681	0.0	0.12376890748114042	4.160483421896887E-4
64-65	6.569184350363505E-5	0.0019105377818973862	0.0	0.1272067806244973	4.160483421896887E-4
66-67	6.569184350363505E-5	0.0019214864224813256	0.0	0.131586236858073	4.324713030655975E-4
68-69	6.569184350363505E-5	0.001954332344233143	0.0	0.1362065631844953	4.3794562335756703E-4
70-71	6.569184350363505E-5	0.00199265258627693	0.0	0.13935429735237784	4.65317224817415E-4
72-73	6.569184350363505E-5	0.0020090755471528386	0.0	0.14353120373515063	5.58380669780898E-4
74-75	6.569184350363505E-5	0.0020583444297805654	0.0	0.14729206177573373	5.58380669780898E-4
76-77	6.569184350363505E-5	0.002195202437079805	0.0	0.15024819473339732	5.638549900728676E-4
78-79	7.664048408757424E-5	0.002271842921167379	0.0	0.1521970527573385	5.693293103648372E-4
80-81	7.664048408757424E-5	0.0022827915617513184	0.0	0.15294703463733833	5.967009118246851E-4
82-83	7.664048408757424E-5	0.0023046888429191963	0.0	0.1543758322335424	6.350211538684723E-4
84-85	7.664048408757424E-5	0.0023320604443790444	0.0	0.15555281109631586	6.350211538684723E-4
86-87	7.664048408757424E-5	0.002364906366130862	0.0	0.1561933065704763	6.350211538684723E-4
88	7.664048408757424E-5	0.002441546850218436	0.0	0.15626994705456387	6.350211538684723E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9795	0.0	49.271114	1
GTATCAA	18250	0.0	38.62431	1
TCAACGC	21520	0.0	32.038258	4
ATCAACG	21950	0.0	31.509373	3
CAACGCA	22155	0.0	31.060915	5
TATCAAC	22855	0.0	30.486622	2
AACGCAG	22655	0.0	30.300756	6
ACGCAGA	26450	0.0	25.807816	7
CGCAGAG	26900	0.0	25.36131	8
GTACATG	26020	0.0	24.54405	1
TACATGG	25980	0.0	24.18695	2
CTCGTAT	2485	0.0	23.73876	9
ACATGGG	26155	0.0	23.403677	3
AATCTCG	2545	0.0	23.177326	6
TCTCGTA	2815	0.0	20.955194	8
GCAGAGT	32560	0.0	20.927107	9
CATGGGG	19715	0.0	20.922197	4
GAGTACT	19445	0.0	19.070448	12-13
CAGAGTA	31265	0.0	18.375795	10-11
AGAGTAC	28880	0.0	18.309557	10-11
>>END_MODULE