FastQCFastQC Report
Fri 27 May 2016
SRR2139351_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139351_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7961904
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87500.046.1040461
GTATCAA154450.035.859431
TCAACGC181150.029.5286834
ATCAACG181750.029.4326823
CAACGCA184700.028.7778645
TATCAAC192150.028.1458262
AACGCAG191950.027.8010656
GTACATG217700.024.6109091
TACATGG217950.024.2163452
ACGCAGA225750.023.5269267
ACATGGG222400.023.1838533
CGCAGAG230350.023.0581178
CATGGGG168650.020.480454
GTGGTAT44400.019.4966551
GCAGAGT274400.019.3571559
GAGTACT156700.018.30234712-13
AGAGTAC241200.017.38728710-11
CAGAGTA263700.017.33793410-11
CTTATAC50500.017.1416131
ATGGGGG102400.017.1108285
Produced by FastQC (version 0.11.5)