##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139351_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7961904
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60544412492288	33.0	33.0	33.0	33.0	33.0
2	32.60776593136516	33.0	33.0	33.0	33.0	33.0
3	32.67024822203332	33.0	33.0	33.0	33.0	33.0
4	36.59062631250013	37.0	37.0	37.0	37.0	37.0
5	36.58916372264725	37.0	37.0	37.0	37.0	37.0
6	36.10361855656637	37.0	37.0	37.0	33.0	37.0
7	35.95891284295816	37.0	37.0	37.0	33.0	37.0
8	35.84423914179322	37.0	37.0	37.0	33.0	37.0
9	35.852482270572466	37.0	37.0	37.0	33.0	37.0
10-11	35.76682436763869	37.0	37.0	37.0	33.0	37.0
12-13	35.677354436828175	37.0	37.0	37.0	33.0	37.0
14-15	37.35123439066837	40.0	37.0	40.0	33.0	40.0
16-17	37.271873599581205	40.0	37.0	40.0	33.0	40.0
18-19	37.29009731843037	40.0	37.0	40.0	33.0	40.0
20-21	37.20445851963977	40.0	37.0	40.0	33.0	40.0
22-23	37.07418820711999	40.0	37.0	40.0	33.0	40.0
24-25	37.05295992029317	40.0	37.0	40.0	33.0	40.0
26-27	36.88473119208721	40.0	37.0	40.0	33.0	40.0
28-29	36.707383794098085	40.0	37.0	40.0	33.0	40.0
30-31	36.5170887852811	40.0	37.0	40.0	33.0	40.0
32-33	36.29840777808464	38.5	37.0	40.0	33.0	40.0
34-35	36.20140456833916	37.0	37.0	40.0	27.0	40.0
36-37	35.98506067371767	37.0	37.0	40.0	27.0	40.0
38-39	36.027257770768244	37.0	37.0	40.0	27.0	40.0
40-41	36.07312641407567	37.0	37.0	40.0	27.0	40.0
42-43	35.98979647031835	37.0	37.0	40.0	27.0	40.0
44-45	35.85165933776847	37.0	37.0	40.0	27.0	40.0
46-47	35.675450500074106	37.0	37.0	40.0	27.0	40.0
48-49	35.49484231564901	37.0	33.0	40.0	27.0	40.0
50-51	35.48740552814119	37.0	33.0	40.0	27.0	40.0
52-53	35.36906712246473	37.0	33.0	40.0	27.0	40.0
54-55	35.1895779575909	37.0	33.0	40.0	27.0	40.0
56-57	34.94297341212881	37.0	33.0	40.0	27.0	40.0
58-59	34.810543555884536	37.0	33.0	40.0	27.0	40.0
60-61	34.68489957996279	37.0	33.0	40.0	27.0	40.0
62-63	34.523548136856846	37.0	33.0	40.0	27.0	40.0
64-65	34.34935827695531	37.0	33.0	40.0	27.0	40.0
66-67	34.10369898753491	37.0	33.0	37.0	27.0	40.0
68-69	33.903142795371096	37.0	33.0	37.0	27.0	40.0
70-71	33.672765749745054	37.0	33.0	37.0	27.0	40.0
72-73	33.374331015806035	37.0	33.0	37.0	22.0	40.0
74-75	33.02391893123168	37.0	33.0	37.0	22.0	38.5
76-77	31.035093574392903	33.0	30.0	35.0	22.0	37.0
78-79	33.11460337863094	35.0	33.0	37.0	27.0	37.0
80-81	33.46447782144118	37.0	33.0	37.0	27.0	37.0
82-83	33.4133827609416	37.0	33.0	37.0	27.0	37.0
84-85	33.31512578350649	37.0	33.0	37.0	27.0	37.0
86-87	33.18635407682564	37.0	33.0	37.0	27.0	37.0
88-89	33.03106868326907	33.0	33.0	37.0	27.0	37.0
90-91	32.96464190689982	33.0	33.0	37.0	27.0	37.0
92-93	33.01743291437879	33.0	33.0	37.0	27.0	37.0
94-95	33.0830163000232	33.0	33.0	37.0	27.0	37.0
96-97	33.21601996573451	33.0	33.0	37.0	27.0	37.0
98-99	33.409607725590725	33.0	33.0	37.0	27.0	37.0
100	33.42948929880784	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	9.0
16	35.0
17	111.0
18	375.0
19	1012.0
20	2686.0
21	6973.0
22	17175.0
23	38055.0
24	73030.0
25	119784.0
26	173424.0
27	225897.0
28	270172.0
29	301414.0
30	320948.0
31	335486.0
32	354219.0
33	388546.0
34	454613.0
35	582714.0
36	912667.0
37	1864959.0
38	1499666.0
39	17932.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.40591145032646	19.401113100584986	13.003447416597838	24.18952803249072
2	15.318494433340124	20.709253333326636	40.9296765356262	23.042575697707047
3	18.98288828797847	25.713161751065076	29.467810771748425	25.83613918920803
4	12.60636632973424	17.261326692152217	38.249060871408844	31.883246106704703
5	13.545666966092531	37.91532779094046	35.22232872940945	13.31667651355756
6	31.327267949977795	37.0667619202643	18.304001153492933	13.301968976264975
7	27.67171018389571	31.888653769249164	22.52934725161218	17.910288795242945
8	25.276014380479843	36.366715800642666	20.740214903369846	17.617054915507644
9	26.200428942624782	16.245297607205515	20.352217258585384	37.202056191584326
10-11	24.898557415931112	26.260469714928348	28.593708807691836	20.247264061448703
12-13	25.80707920919545	23.731823282902724	28.577915826453054	21.883181681448775
14-15	22.758999487732552	25.172936578627464	25.99792141376521	26.07014251987478
16-17	22.561695041788997	28.13978917605638	26.676408055158667	22.622107726995957
18-19	22.534697730593084	27.33182289060506	28.285715326384242	21.847764052417613
20-21	23.304725695288756	26.594057427649947	28.159183629871325	21.942033247189975
22-23	22.938797841699472	26.598783754889503	28.022444564629527	22.43997383878149
24-25	22.771625148435323	26.71066317545864	28.17479683141513	22.342914844690906
26-27	22.768073269910825	26.909900575245025	28.010735046531998	22.31129110831215
28-29	22.607690566851613	26.88305657276603	28.105633025562827	22.403619834819533
30-31	22.893057551864597	26.793325437558913	27.983520724629912	22.33009628594658
32-33	22.67520405659001	27.01145956853484	27.944888740812523	22.368447634062626
34-35	22.79581662750792	27.045786292067064	27.965001349095132	22.19339573132988
36-37	22.85846230978297	27.008840920663747	27.79676416412932	22.335932605423963
38-39	22.65734621937466	27.098889578607675	27.933804837249316	22.30995936476835
40-41	22.84114987180039	26.965214959737015	27.885225760146408	22.308409408316184
42-43	22.727135666986577	27.038489757145882	27.745776728104104	22.488597847763437
44-45	22.788969841535984	27.172506909798788	27.52436356561435	22.514159683050877
46-47	22.88608509862296	27.063070782595073	27.250705200279274	22.800138918502697
48-49	22.740655187671145	27.17436437430037	27.350336577280604	22.73464386074788
50-51	22.72683542460402	27.321839158947547	27.26960499928099	22.681720417167448
52-53	22.882496218040423	27.24373266750944	27.182436196023037	22.6913349184271
54-55	22.8019466410675	27.187864379637457	27.20260354348455	22.807585435810495
56-57	22.839685482223388	27.391178834790047	27.108435946816655	22.660699736169914
58-59	22.734331771439685	27.448843257263245	27.159209171680782	22.657615799616288
60-61	22.76736572955761	27.475972780319445	27.177652470254998	22.579009019867943
62-63	22.73109576881307	27.63635633032237	27.086017113352394	22.546530787512165
64-65	22.69927128560244	27.563097952931297	27.092149320436036	22.645481441030224
66-67	22.76853048882538	27.52499999092395	27.041355221871406	22.665114298379272
68-69	22.702141114228912	27.50460159558159	27.12250360827135	22.67075368191815
70-71	22.706399708917537	27.242461883634622	27.195987216100843	22.855151191346998
72-73	22.683741333357	27.292092110784772	27.185850248493047	22.83831630736518
74-75	22.594232907343077	27.41083927061404	27.130895654884352	22.864032167158534
76-77	22.669774891398298	27.323473260027402	27.207577892917044	22.799173955657253
78-79	22.638044204208754	27.299759065003563	27.195163457560795	22.867033273226888
80-81	22.594755507686934	27.433182300550886	27.171520688690336	22.800541503071848
82-83	22.58277673292992	27.32993655769407	27.32299120571232	22.764295503663693
84-85	22.627891532011326	27.323166767590518	27.30419181007723	22.744749890320925
86-87	22.524766476852882	27.36836500651325	27.353720932079533	22.753147584554334
88-89	22.567119961941255	27.26023825079252	27.32627387910991	22.846367908156324
90-91	22.550517844415293	27.284349002053354	27.397529059875954	22.7676040936554
92-93	22.58223413584505	27.249425819083473	27.415725352503806	22.752614692567676
94-95	22.417221508173935	27.312592024984017	27.509238695823594	22.76094777101845
96-97	22.421635112875514	27.41647320873433	27.59334331772284	22.568548360667315
98-99	22.608672563180857	28.117342931629384	26.72601000797942	22.547974497210337
100	20.7802252233435	27.6080413605702	27.937780111835863	23.67395330425044
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	979.0
1	897.0
2	1253.0
3	1892.0
4	2844.5
5	5208.5
6	7283.5
7	8151.0
8	8217.5
9	7464.5
10	6520.0
11	5799.5
12	6204.5
13	8686.5
14	14554.0
15	23189.0
16	31169.5
17	36564.0
18	38946.0
19	38137.0
20	35806.0
21	32691.0
22	30200.0
23	30562.0
24	33730.0
25	39841.0
26	48595.5
27	58973.5
28	71265.5
29	85865.5
30	101827.5
31	118762.0
32	137216.0
33	156189.0
34	176238.0
35	196487.0
36	217358.5
37	237590.0
38	255056.5
39	269905.5
40	284160.0
41	298543.5
42	309620.0
43	320058.5
44	329193.5
45	338045.0
46	346259.0
47	352251.0
48	356880.5
49	356937.5
50	354545.5
51	348199.0
52	335959.5
53	320169.0
54	303525.0
55	284888.5
56	264229.5
57	242277.0
58	217428.0
59	192154.0
60	165452.5
61	138314.0
62	114009.5
63	92094.5
64	72216.0
65	55987.5
66	43577.0
67	34564.5
68	27691.5
69	21577.0
70	16668.0
71	12952.0
72	9992.5
73	7467.0
74	5408.0
75	3988.5
76	3016.0
77	2253.5
78	1668.5
79	1218.5
80	880.5
81	643.5
82	495.0
83	355.5
84	252.5
85	190.5
86	150.0
87	122.5
88	86.5
89	55.5
90	35.0
91	20.0
92	13.0
93	8.5
94	5.0
95	3.0
96	3.0
97	2.5
98	1.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.77272772944763E-4
3	0.0035293065578283784
4	0.0033660290302420124
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.3815790795769454E-4
12-13	0.0017458135641926858
14-15	0.003045753879976448
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.006025837051417517
26-27	0.01862447407311696
28-29	0.007865780797078866
30-31	0.0
32-33	0.0
34-35	1.7919637593249315E-4
36-37	0.01422972590384319
38-39	0.041107658191308266
40-41	0.046888545142065405
42-43	0.008208077847372394
44-45	3.0317605921476606E-4
46-47	0.0
48-49	0.03231385512541136
50-51	0.04722911275763146
52-53	0.04831049177587447
54-55	0.027753572173809866
56-57	9.560224809379163E-4
58-59	0.006369865877614389
60-61	0.030170335331912157
62-63	0.03612335184536414
64-65	0.01840331912232049
66-67	0.04750690014863869
68-69	0.06269886092549821
70-71	0.0722449836709632
72-73	0.04829486118525831
74-75	0.021314395223771078
76-77	0.004735920883903181
78-79	4.124535932422381E-4
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.002079602271838677
92-93	0.0024693480262717835
94-95	6.147955083218363E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2315.0
20-21	7170.0
22-23	8280.0
24-25	14517.0
26-27	19909.0
28-29	27928.0
30-31	28636.0
32-33	31331.0
34-35	36096.0
36-37	37660.0
38-39	40832.0
40-41	48378.0
42-43	56417.0
44-45	66023.0
46-47	67810.0
48-49	53194.0
50-51	61581.0
52-53	55413.0
54-55	64318.0
56-57	65381.0
58-59	67098.0
60-61	66881.0
62-63	61803.0
64-65	60443.0
66-67	75387.0
68-69	56024.0
70-71	60340.0
72-73	57037.0
74-75	53602.0
76-77	47011.0
78-79	68563.0
80-81	84988.0
82-83	100382.0
84-85	110142.0
86-87	120119.0
88-89	128537.0
90-91	142414.0
92-93	154907.0
94-95	175607.0
96-97	341748.0
98-99	108672.0
100-101	5027010.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.21258739917346
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.37856450182731	79.69361043026623
2	5.996338315559168	10.45912358841433
3	1.4770007939011294	3.8643918248025244
4	0.5446869824979759	1.9001424426518712
5	0.23393139569795723	1.0200881146359364
6	0.12270373621633628	0.6420786191383412
7	0.06911747644490597	0.4219539768683173
8	0.04689234496177487	0.3271682186664786
9	0.0314204715621937	0.24662345600169563
>10	0.09755627460321523	1.2719589232881692
>50	0.0015097401715923019	0.08594382550782827
>100	2.343025910656903E-4	0.03807064404669677
>500	4.366396529752088E-5	0.028845935711654395
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.2559809814335867E-5	0.0	0.0	0.0
3	0.0	1.2559809814335867E-5	0.0	0.0	0.0
4	0.0	3.76794294430076E-5	0.0	0.0	0.0
5	1.2559809814335867E-5	3.76794294430076E-5	0.0	0.0	0.0
6	1.2559809814335867E-5	3.76794294430076E-5	0.0	2.5119619628671734E-5	1.2559809814335867E-5
7	1.2559809814335867E-5	3.76794294430076E-5	0.0	3.76794294430076E-5	1.2559809814335867E-5
8	1.2559809814335867E-5	3.76794294430076E-5	0.0	3.76794294430076E-5	1.2559809814335867E-5
9	1.2559809814335867E-5	7.53588588860152E-5	0.0	3.76794294430076E-5	1.2559809814335867E-5
10-11	2.5119619628671734E-5	1.130382883290228E-4	0.0	5.023923925734347E-5	1.2559809814335867E-5
12-13	2.5119619628671734E-5	1.130382883290228E-4	0.0	5.023923925734347E-5	1.2559809814335867E-5
14-15	3.76794294430076E-5	1.695574324935342E-4	0.0	5.023923925734347E-5	4.3959334350175534E-5
16-17	3.76794294430076E-5	1.8839714721503802E-4	0.0	5.65191441645114E-5	5.023923925734347E-5
18-19	6.907895397884727E-5	1.9467705212220594E-4	0.0	6.279904907167934E-5	6.279904907167934E-5
20-21	9.4198573607519E-5	2.1351676684370975E-4	0.0	7.53588588860152E-5	8.791866870035107E-5
22-23	1.130382883290228E-4	2.386363864723815E-4	0.0	8.163876379318314E-5	1.1931819323619073E-4
24-25	1.1931819323619075E-4	2.574761011938853E-4	1.2559809814335867E-5	1.3815790795769454E-4	1.507177177720304E-4
26-27	1.255980981433587E-4	2.6375600610105323E-4	1.2559809814335867E-5	2.009569570293739E-4	1.5699762267919833E-4
28-29	1.3815790795769454E-4	2.763158159153891E-4	1.2559809814335867E-5	4.521531533160912E-4	1.7583733740070213E-4
30-31	1.3815790795769454E-4	3.139952453583967E-4	1.2559809814335867E-5	0.0011555025029188998	1.8839714721503802E-4
32-33	1.4443781286486247E-4	3.139952453583967E-4	1.2559809814335867E-5	0.0026124404413818605	2.072368619365418E-4
34-35	1.5699762267919833E-4	3.7679429443007603E-4	1.2559809814335867E-5	0.0042389358123383555	2.1351676684370975E-4
36-37	1.6327752758636628E-4	4.584330582232592E-4	1.2559809814335867E-5	0.006688098726133849	2.1351676684370975E-4
38-39	1.6327752758636628E-4	5.086722974806026E-4	1.2559809814335867E-5	0.011730862366589701	2.1979667175087768E-4
40-41	1.9467705212220594E-4	5.714713465522819E-4	1.2559809814335867E-5	0.016679427433438032	2.260765766580456E-4
42-43	2.1351676684370975E-4	6.028708710881217E-4	1.2559809814335867E-5	0.02176615040824406	2.3235648156521356E-4
44-45	2.1351676684370975E-4	6.970694446956406E-4	1.2559809814335867E-5	0.027700660545517758	2.6375600610105323E-4
46-47	2.1351676684370975E-4	7.787082084888238E-4	1.2559809814335867E-5	0.03436363965202294	2.82595720822557E-4
48-49	2.1351676684370975E-4	7.912680183031597E-4	1.2559809814335867E-5	0.03980203730163036	3.014354355440608E-4
50-51	2.1979667175087768E-4	8.917464968178465E-4	1.2559809814335867E-5	0.04395933435017553	3.0771534045122875E-4
52-53	2.260765766580456E-4	9.168661164465183E-4	1.2559809814335867E-5	0.04951077028811199	3.4539476989423637E-4
54-55	2.260765766580456E-4	9.231460213536863E-4	1.2559809814335867E-5	0.05623026853878168	3.7679429443007603E-4
56-57	2.260765766580456E-4	9.54545545889526E-4	1.2559809814335867E-5	0.06237201553799192	3.9563400915157984E-4
58-59	2.386363864723815E-4	9.796651655181977E-4	1.2559809814335867E-5	0.06764085575510581	4.270335336874195E-4
60-61	2.386363864723815E-4	0.0010110646900540372	1.2559809814335867E-5	0.07168511451532197	4.270335336874195E-4
62-63	2.386363864723815E-4	0.0010675838342185488	1.2559809814335867E-5	0.07643272262514092	4.521531533160912E-4
64-65	2.449162913795494E-4	0.0011178230734758922	1.2559809814335867E-5	0.08043930195591406	4.521531533160912E-4
66-67	2.511961962867174E-4	0.0011303828832902282	1.2559809814335867E-5	0.08529994835406204	4.521531533160912E-4
68-69	2.574761011938853E-4	0.0011429426931045638	1.2559809814335867E-5	0.09056878857117594	4.521531533160912E-4
70-71	2.6375600610105323E-4	0.0011492225980117318	1.2559809814335867E-5	0.09392853769651079	4.521531533160912E-4
72-73	2.700359110082212E-4	0.0011617824078260677	1.2559809814335867E-5	0.09939833487065405	5.526316318307782E-4
74-75	2.763158159153891E-4	0.0011743422176404036	1.2559809814335867E-5	0.1034049142014272	5.526316318307782E-4
76-77	2.763158159153891E-4	0.0012245814568977473	1.2559809814335867E-5	0.10646322789121798	5.526316318307782E-4
78-79	2.763158159153891E-4	0.0012559809814335868	1.2559809814335867E-5	0.10827812040938951	5.526316318307782E-4
80-81	2.763158159153891E-4	0.0012559809814335868	1.2559809814335867E-5	0.10900030947371384	5.777512514594499E-4
82-83	2.763158159153891E-4	0.0012559809814335868	1.2559809814335867E-5	0.11062052493976315	6.279904907167934E-4
84-85	2.763158159153891E-4	0.0012622608863407546	1.2559809814335867E-5	0.1121779413567408	6.279904907167934E-4
86-87	2.763158159153891E-4	0.0012936604108765943	1.2559809814335867E-5	0.11306340794865148	6.342703956239613E-4
88	2.763158159153891E-4	0.0013062202206909302	1.2559809814335867E-5	0.1131764462369805	6.405503005311292E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8750	0.0	46.104046	1
GTATCAA	15445	0.0	35.85943	1
TCAACGC	18115	0.0	29.528683	4
ATCAACG	18175	0.0	29.432682	3
CAACGCA	18470	0.0	28.777864	5
TATCAAC	19215	0.0	28.145826	2
AACGCAG	19195	0.0	27.801065	6
GTACATG	21770	0.0	24.610909	1
TACATGG	21795	0.0	24.216345	2
ACGCAGA	22575	0.0	23.526926	7
ACATGGG	22240	0.0	23.183853	3
CGCAGAG	23035	0.0	23.058117	8
CATGGGG	16865	0.0	20.48045	4
GTGGTAT	4440	0.0	19.496655	1
GCAGAGT	27440	0.0	19.357155	9
GAGTACT	15670	0.0	18.302347	12-13
AGAGTAC	24120	0.0	17.387287	10-11
CAGAGTA	26370	0.0	17.337934	10-11
CTTATAC	5050	0.0	17.141613	1
ATGGGGG	10240	0.0	17.110828	5
>>END_MODULE