FastQCFastQC Report
Fri 27 May 2016
SRR2139350_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139350_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7979448
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93950.047.382431
GTATCAA171500.038.053551
TCAACGC209000.030.6822644
ATCAACG209850.030.4386793
CAACGCA213750.029.86165
TATCAAC219400.029.4201932
AACGCAG220750.029.0110746
GTACATG258300.025.2333891
ACGCAGA254300.024.9524257
CGCAGAG256950.024.728398
TACATGG260750.024.5131232
ACATGGG265650.023.5233213
GCAGAGT305750.020.7135289
CATGGGG188250.020.5010054
GAGTACT170600.019.15182712-13
AGAGTAC274350.018.41534210-11
GTGGTAT44250.018.0321961
CAGAGTA296100.017.94201910-11
ATGGGGG114400.017.1421975
AGTACTT190550.016.6838612-13
Produced by FastQC (version 0.11.5)