##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139350_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7979448
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.631157192828375	33.0	33.0	33.0	33.0	33.0
2	32.631032121520185	33.0	33.0	33.0	33.0	33.0
3	32.685546168105866	33.0	33.0	33.0	33.0	33.0
4	36.61156435883785	37.0	37.0	37.0	37.0	37.0
5	36.61819000512316	37.0	37.0	37.0	37.0	37.0
6	36.19071707717125	37.0	37.0	37.0	33.0	37.0
7	36.0628751512636	37.0	37.0	37.0	33.0	37.0
8	35.964319712341	37.0	37.0	37.0	33.0	37.0
9	35.96916252853581	37.0	37.0	37.0	33.0	37.0
10-11	35.89416980974123	37.0	37.0	37.0	33.0	37.0
12-13	35.80800764664423	37.0	37.0	37.0	33.0	37.0
14-15	37.54896240942982	40.0	37.0	40.0	33.0	40.0
16-17	37.48356089293395	40.0	37.0	40.0	33.0	40.0
18-19	37.50979591570745	40.0	37.0	40.0	33.0	40.0
20-21	37.431456146835544	40.0	37.0	40.0	33.0	40.0
22-23	37.30901130720055	40.0	37.0	40.0	33.0	40.0
24-25	37.29513061589117	40.0	37.0	40.0	33.0	40.0
26-27	37.14728039829551	40.0	37.0	40.0	33.0	40.0
28-29	36.985073081908254	40.0	37.0	40.0	33.0	40.0
30-31	36.799281098054536	40.0	37.0	40.0	33.0	40.0
32-33	36.59054109231826	40.0	37.0	40.0	33.0	40.0
34-35	36.50049842348008	40.0	37.0	40.0	33.0	40.0
36-37	36.309231496718354	40.0	37.0	40.0	30.0	40.0
38-39	36.34540004557745	40.0	37.0	40.0	33.0	40.0
40-41	36.38490499848367	40.0	37.0	40.0	33.0	40.0
42-43	36.3014863228607	40.0	37.0	40.0	33.0	40.0
44-45	36.17154547509796	38.5	37.0	40.0	27.0	40.0
46-47	36.005382162076046	37.0	37.0	40.0	27.0	40.0
48-49	35.81962252927746	37.0	37.0	40.0	27.0	40.0
50-51	35.81117674700211	37.0	37.0	40.0	27.0	40.0
52-53	35.70396702127846	37.0	37.0	40.0	27.0	40.0
54-55	35.52372927455272	37.0	35.0	40.0	27.0	40.0
56-57	35.29277616034428	37.0	33.0	40.0	27.0	40.0
58-59	35.17317704765607	37.0	33.0	40.0	27.0	40.0
60-61	35.05060279952569	37.0	33.0	40.0	27.0	40.0
62-63	34.89248644766712	37.0	33.0	40.0	27.0	40.0
64-65	34.724440046655836	37.0	33.0	40.0	27.0	40.0
66-67	34.47006944572625	37.0	33.0	38.5	27.0	40.0
68-69	34.27045144297426	37.0	33.0	37.0	27.0	40.0
70-71	34.04507665993812	37.0	33.0	37.0	27.0	40.0
72-73	33.75829002515239	37.0	33.0	37.0	27.0	40.0
74-75	33.41724291430269	37.0	33.0	37.0	24.5	40.0
76-77	31.32110634703676	33.0	30.0	35.0	24.5	37.0
78-79	33.405064044991086	37.0	33.0	37.0	27.0	37.0
80-81	33.75044230951372	37.0	33.0	37.0	27.0	37.0
82-83	33.699231885042096	37.0	33.0	37.0	27.0	37.0
84-85	33.60163781999398	37.0	33.0	37.0	27.0	37.0
86-87	33.47012558508584	37.0	33.0	37.0	27.0	37.0
88-89	33.31658944466443	37.0	33.0	37.0	27.0	37.0
90-91	33.238712848291655	35.0	33.0	37.0	27.0	37.0
92-93	33.28119515935212	33.0	33.0	37.0	27.0	37.0
94-95	33.33504683095437	33.0	33.0	37.0	27.0	37.0
96-97	33.44650174397654	33.0	33.0	37.0	27.0	37.0
98-99	33.58765345807382	33.0	33.0	37.0	27.0	37.0
100	33.5627968311527	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	2.0
14	1.0
15	18.0
16	28.0
17	115.0
18	342.0
19	985.0
20	2349.0
21	6081.0
22	14855.0
23	32857.0
24	62504.0
25	103327.0
26	150164.0
27	196366.0
28	236779.0
29	267839.0
30	289783.0
31	309648.0
32	332173.0
33	371955.0
34	440083.0
35	573207.0
36	918321.0
37	1946941.0
38	1696657.0
39	26067.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.00894535673964	19.854308199553177	13.089152669351648	24.04759377435554
2	15.021760378052967	21.40270754320087	40.994090917236875	22.58144116150929
3	18.204489746913147	26.938744664537285	30.173948178492015	24.682817410057552
4	12.31482340202077	17.894735522875898	39.124918224269784	30.665522850833547
5	13.071145540680392	38.39804166855694	35.53538679251667	12.995425998245993
6	29.88297309324819	38.015090519430736	19.036181329357788	13.06575505796329
7	26.6128717942484	32.727543650581296	23.46356834001153	17.196016215158767
8	24.9917694797647	36.279243411228876	21.410293219567723	17.318693889438705
9	26.490971116168826	16.057340815723194	20.843850457306125	36.60783761080185
10-11	24.439824095326042	26.507545552564466	29.168272617781042	19.88435773432845
12-13	25.468727343774578	24.163666097319833	29.02675027781193	21.34085628109366
14-15	22.562416932513955	25.374532926525887	26.530255833757877	25.532794307202284
16-17	22.08194727254316	28.370978794523126	27.22475289017486	22.322321042758848
18-19	22.13333553899969	27.584113587807074	28.908327994618173	21.374222878575058
20-21	22.99040305441195	26.89297577909553	28.600644427157018	21.515976739335503
22-23	22.67824227888717	26.89548025487745	28.430207122908964	21.996070343326412
24-25	22.40243982179713	26.99277102167663	28.629684971716042	21.975104184810196
26-27	22.391681063378822	27.158772120766123	28.512487838074165	21.93705897778089
28-29	22.310317644494937	27.23654627246389	28.498560991026494	21.95457509201468
30-31	22.52775922247214	27.17678419232267	28.3213374706496	21.974119114555585
32-33	22.381852977011356	27.304067890201583	28.29606520940927	22.018013923377787
34-35	22.47777991560357	27.291070239311566	28.335282992191395	21.895866852893473
36-37	22.491893130263957	27.3048585353153	28.189406818534763	22.01384151588598
38-39	22.31684064340784	27.44047514438193	28.29328981066209	21.94939440154814
40-41	22.503731019918686	27.28786969846162	28.22394002563869	21.984459255981008
42-43	22.413869439707305	27.36110271911192	28.03208429132345	22.19294354985733
44-45	22.489041061923825	27.456592267154463	27.814305020376352	22.240061650545364
46-47	22.546587558061994	27.43209927844917	27.554300924929098	22.467012238559743
48-49	22.50483451365033	27.580913719076726	27.53372283438341	22.380528932889533
50-51	22.46268463051979	27.6613357683382	27.468968680328025	22.40701092081398
52-53	22.573170574546115	27.584453673501642	27.374059656969372	22.46831609498287
54-55	22.497510911328938	27.532422444634236	27.411895631248413	22.558171012788414
56-57	22.54047996801896	27.6585911003352	27.31309545872423	22.487833472921608
58-59	22.484515171415502	27.692550333312273	27.363275022523737	22.45965947274849
60-61	22.52948388488374	27.707945795044125	27.380456581350515	22.382113738721614
62-63	22.522272062685424	27.884948968728263	27.278968586313113	22.313810382273196
64-65	22.522393271054668	27.774436204227378	27.25088116221724	22.452289362500714
66-67	22.54802547925598	27.698022410442768	27.246414699731105	22.50753741057015
68-69	22.44216679379538	27.73709163480894	27.38187210620118	22.438869465194504
70-71	22.52011749317625	27.494644326376093	27.411504992192377	22.573733188255282
72-73	22.55014521113243	27.52776235104885	27.30246561953888	22.61962681827984
74-75	22.401711282540244	27.641369494366774	27.30739590363356	22.649523319459423
76-77	22.520548405623586	27.579628359982923	27.306845668432654	22.59297756596084
78-79	22.38422677336881	27.5649194967942	27.357772683713154	22.693081046123844
80-81	22.382678450275495	27.624971087373474	27.369327477826573	22.623022984524457
82-83	22.455378808494032	27.552623606396075	27.445199501964133	22.546798083145756
84-85	22.48415979454855	27.549125507232493	27.401242021118794	22.565472677100164
86-87	22.327345205732744	27.55021577173144	27.523774604783817	22.598664417751994
88-89	22.381271513824373	27.47119745231239	27.480808002913548	22.666723030949687
90-91	22.408676714644425	27.440514035203222	27.525559482499762	22.62524976765259
92-93	22.406898304672005	27.430373536843987	27.576267747898616	22.586460410585392
94-95	22.319354520614972	27.426846745543454	27.611752666331075	22.6420460675105
96-97	22.36384457263164	27.490912564138114	27.694696887330945	22.4505459758993
98-99	23.385055630002615	29.41125007367098	28.004333179227576	19.199361117098825
100	22.53993119799855	30.230867115069987	21.6367268140839	25.592474872847564
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1184.0
1	1152.5
2	1750.5
3	2591.0
4	3782.0
5	6861.0
6	9500.0
7	10499.0
8	10387.5
9	9319.0
10	8066.0
11	7012.0
12	7532.0
13	10756.0
14	18055.0
15	28287.0
16	37462.5
17	43743.0
18	46309.0
19	44288.5
20	40167.5
21	36112.5
22	33646.5
23	34281.5
24	38208.5
25	45842.5
26	56261.5
27	68729.0
28	83155.0
29	99614.0
30	116841.0
31	135189.5
32	155317.0
33	174973.5
34	194483.0
35	213464.5
36	233161.0
37	252682.5
38	268730.5
39	281904.5
40	294402.5
41	306919.0
42	316763.5
43	325037.0
44	332756.0
45	340448.0
46	345907.0
47	348092.5
48	347963.0
49	343533.5
50	337829.0
51	330493.5
52	317894.5
53	300665.0
54	283600.0
55	265262.0
56	244568.5
57	222794.5
58	199685.5
59	175930.5
60	149840.5
61	124466.0
62	101684.0
63	81295.5
64	63443.5
65	48619.5
66	37305.5
67	29519.5
68	23737.0
69	18118.0
70	13600.0
71	10397.5
72	7823.5
73	5751.0
74	4244.5
75	3182.5
76	2385.5
77	1735.0
78	1212.0
79	861.5
80	623.5
81	457.0
82	366.0
83	278.0
84	200.0
85	145.5
86	106.5
87	77.5
88	55.0
89	36.0
90	23.0
91	16.5
92	13.0
93	8.0
94	4.0
95	2.5
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.2532195209493187E-5
2	4.6369122275124796E-4
3	0.0036343366107530247
4	0.0038599161245239017
5	2.5064390418986375E-5
6	1.2532195209493187E-5
7	1.2532195209493187E-5
8	1.2532195209493187E-5
9	1.2532195209493187E-5
10-11	1.5665244011866484E-4
12-13	0.001616653182024621
14-15	0.0030453234359068444
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.006362457855782613
26-27	0.019837756970678248
28-29	0.00897131224179087
30-31	0.0
32-33	0.0
34-35	1.2754661972440873E-4
36-37	0.013022854308595013
38-39	0.0374908324103806
40-41	0.042503806743148076
42-43	0.00763685945515793
44-45	3.3454282590948914E-4
46-47	6.6169394841844226E-6
48-49	0.03452222468259029
50-51	0.055824051834392596
52-53	0.05327720572817752
54-55	0.02910071740516233
56-57	0.0013380431532711188
58-59	0.005983679253615772
60-61	0.029494894131909526
62-63	0.03411330384793527
64-65	0.016618486879618796
66-67	0.04379101881147283
68-69	0.06009806835674856
70-71	0.07238586963606088
72-73	0.05012174919109396
74-75	0.022668558289447548
76-77	0.0053440901440519935
78-79	3.9684602046244924E-4
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.002332504815228011
92-93	0.0025651071421855355
94-95	5.260326890978299E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2071.0
20-21	6459.0
22-23	7259.0
24-25	12667.0
26-27	17864.0
28-29	26157.0
30-31	27557.0
32-33	30304.0
34-35	34472.0
36-37	35404.0
38-39	39468.0
40-41	46673.0
42-43	54951.0
44-45	63891.0
46-47	64534.0
48-49	52249.0
50-51	63208.0
52-53	66670.0
54-55	61156.0
56-57	68726.0
58-59	78225.0
60-61	59221.0
62-63	53049.0
64-65	71494.0
66-67	78751.0
68-69	52980.0
70-71	58264.0
72-73	67082.0
74-75	61506.0
76-77	48319.0
78-79	70351.0
80-81	89490.0
82-83	88166.0
84-85	109394.0
86-87	122487.0
88-89	135617.0
90-91	146055.0
92-93	155875.0
94-95	178329.0
96-97	334906.0
98-99	506305.0
100-101	4631842.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.89175896871535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.15203728419216	79.20360853205382
2	6.136399902213678	10.664051624775988
3	1.5198987288387695	3.962000220093457
4	0.5690207484472856	1.9777285488911822
5	0.24929746307116316	1.0830947536345863
6	0.1317690697704309	0.6869787750014472
7	0.07250174040984242	0.4409862626753079
8	0.043411034087793905	0.3017648888431419
9	0.029902761115077624	0.2338473160179359
>10	0.09385331639402986	1.2785466999715305
>50	0.0015337012581811318	0.08903108489925805
>100	3.300099423364388E-4	0.05523895247623003
>500	4.4240259212811084E-5	0.02312234066607176
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.2532195209493187E-5	0.0	1.2532195209493187E-5	0.0
3	0.0	1.2532195209493187E-5	0.0	1.2532195209493187E-5	0.0
4	0.0	2.5064390418986375E-5	0.0	2.5064390418986375E-5	0.0
5	0.0	2.5064390418986375E-5	0.0	2.5064390418986375E-5	0.0
6	8.772536646645232E-5	2.5064390418986375E-5	0.0	2.5064390418986375E-5	1.2532195209493187E-5
7	8.772536646645232E-5	2.5064390418986375E-5	0.0	7.519317125695913E-5	1.2532195209493187E-5
8	8.772536646645232E-5	3.7596585628479566E-5	0.0	7.519317125695913E-5	1.2532195209493187E-5
9	8.772536646645232E-5	1.3785414730442506E-4	0.0	8.772536646645232E-5	1.2532195209493187E-5
10-11	8.772536646645232E-5	1.3785414730442506E-4	0.0	1.1278975688543869E-4	1.2532195209493187E-5
12-13	8.772536646645232E-5	1.3785414730442506E-4	0.0	1.3785414730442506E-4	1.2532195209493187E-5
14-15	8.772536646645232E-5	1.8798292814239782E-4	0.0	1.3785414730442506E-4	1.2532195209493187E-5
16-17	1.2532195209493188E-4	1.8798292814239782E-4	0.0	1.4412024490917166E-4	1.2532195209493187E-5
18-19	1.2532195209493188E-4	2.3184561137562396E-4	0.0	1.5038634251391826E-4	1.879829281423978E-5
20-21	1.2532195209493188E-4	2.757082946088501E-4	0.0	1.5038634251391826E-4	3.7596585628479566E-5
22-23	1.3158804969967848E-4	3.195709778420763E-4	0.0	1.6918463532815804E-4	4.386268323322616E-5
24-25	1.3785414730442506E-4	3.7596585628479564E-4	0.0	2.4437780658511716E-4	5.012878083797275E-5
26-27	1.3785414730442506E-4	4.135624419132752E-4	0.0	3.8849805149428885E-4	5.012878083797275E-5
28-29	1.3785414730442506E-4	4.135624419132752E-4	0.0	0.0012845500089730517	5.012878083797275E-5
30-31	1.3785414730442506E-4	4.6369122275124796E-4	0.0	0.0032207741688397495	5.012878083797275E-5
32-33	1.3785414730442506E-4	4.6995732035599457E-4	0.0	0.007801291517909509	5.012878083797275E-5
34-35	1.3785414730442506E-4	5.702148820319401E-4	0.0	0.013772882535233014	5.012878083797275E-5
36-37	1.3785414730442506E-4	6.64206346103139E-4	0.0	0.021135547220810263	5.012878083797275E-5
38-39	1.3785414730442506E-4	7.018029317316186E-4	0.0	0.03357375096623225	5.012878083797275E-5
40-41	1.3785414730442506E-4	7.519317125695913E-4	0.0	0.04691427276673775	5.012878083797275E-5
42-43	1.3785414730442506E-4	7.581978101743379E-4	0.0	0.05915196138880785	5.012878083797275E-5
44-45	1.5665244011866484E-4	7.957943958028174E-4	0.0	0.07380836368631014	5.012878083797275E-5
46-47	1.7545073293290464E-4	8.271248838265504E-4	0.0	0.08704236182753494	5.012878083797275E-5
48-49	1.7545073293290464E-4	8.33390981431297E-4	0.0	0.09709318238554848	5.012878083797275E-5
50-51	2.00515123351891E-4	9.524468359214822E-4	0.0	0.10582812244656523	5.012878083797275E-5
52-53	2.130473185613842E-4	9.649790311309754E-4	0.0	0.1166371408147531	5.6394878442719345E-5
54-55	2.130473185613842E-4	9.649790311309754E-4	0.0	0.12616787527157267	6.266097604746594E-5
56-57	2.2557951377087738E-4	9.775112263404686E-4	0.0	0.1331232436128414	7.519317125695913E-5
58-59	2.2557951377087738E-4	9.775112263404686E-4	0.0	0.13904470584932693	8.772536646645232E-5
60-61	2.694421970041035E-4	0.001002575616759455	0.0	0.14410771271396217	8.772536646645232E-5
62-63	2.882404898183433E-4	0.0010151078119689481	0.0	0.1491707195785974	8.772536646645232E-5
64-65	2.882404898183433E-4	0.0010527043975974279	0.0	0.1535381896091058	8.772536646645232E-5
66-67	2.882404898183433E-4	0.0010527043975974279	0.0	0.15855733379050782	8.772536646645232E-5
68-69	2.882404898183433E-4	0.001077768788016414	0.0	0.16380205748568072	8.772536646645232E-5
70-71	2.945065874230899E-4	0.0011153653736448938	0.0	0.1675805143413429	8.772536646645232E-5
72-73	3.258370754468229E-4	0.0011153653736448938	0.0	0.17333279194250029	1.1278975688543869E-4
74-75	3.258370754468229E-4	0.0011466958616686267	0.0	0.1784459275879735	1.1278975688543869E-4
76-77	3.258370754468229E-4	0.0011842924472971062	0.0	0.18251889103105878	1.1278975688543869E-4
78-79	3.321031730515695E-4	0.0011905585449018528	0.0	0.18470575909511536	1.1278975688543869E-4
80-81	3.383692706563161E-4	0.0011905585449018528	0.0	0.18578352788313174	1.1278975688543869E-4
82-83	3.383692706563161E-4	0.0011905585449018528	0.0	0.18768842155497473	1.1278975688543869E-4
84-85	3.509014658658093E-4	0.001203090740111346	0.0	0.18925494595616138	1.1278975688543869E-4
86-87	3.509014658658093E-4	0.001234421228135079	0.0	0.1901321996208259	1.1278975688543869E-4
88	3.6343366107530244E-4	0.001340944887415771	0.0	0.1902261910848971	1.1278975688543869E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9395	0.0	47.38243	1
GTATCAA	17150	0.0	38.05355	1
TCAACGC	20900	0.0	30.682264	4
ATCAACG	20985	0.0	30.438679	3
CAACGCA	21375	0.0	29.8616	5
TATCAAC	21940	0.0	29.420193	2
AACGCAG	22075	0.0	29.011074	6
GTACATG	25830	0.0	25.233389	1
ACGCAGA	25430	0.0	24.952425	7
CGCAGAG	25695	0.0	24.72839	8
TACATGG	26075	0.0	24.513123	2
ACATGGG	26565	0.0	23.523321	3
GCAGAGT	30575	0.0	20.713528	9
CATGGGG	18825	0.0	20.501005	4
GAGTACT	17060	0.0	19.151827	12-13
AGAGTAC	27435	0.0	18.415342	10-11
GTGGTAT	4425	0.0	18.032196	1
CAGAGTA	29610	0.0	17.942019	10-11
ATGGGGG	11440	0.0	17.142197	5
AGTACTT	19055	0.0	16.68386	12-13
>>END_MODULE