FastQCFastQC Report
Fri 27 May 2016
SRR2139349_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139349_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7690822
Sequences flagged as poor quality0
Sequence length19-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83750.047.726811
GTATCAA152150.037.1549841
TCAACGC182350.030.1016314
ATCAACG187550.029.3800373
CAACGCA188850.028.9982365
TATCAAC193250.028.92672
AACGCAG195000.028.2137286
GTACATG265950.027.4515291
TACATGG260950.027.3976762
ACATGGG264300.026.4461123
CATGGGG207900.023.9965524
ACGCAGA227600.023.9509727
CGCAGAG235250.023.190748
GCAGAGT275450.019.7910849
ATGGGGG121550.019.5015135
GTGGTAT40850.019.4462781
GAGTACT144650.018.04429212-13
AGAGTAC240700.017.87245210-11
CAGAGTA264200.017.46794910-11
GTATAAT50200.017.4151131
Produced by FastQC (version 0.11.5)