##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139349_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7690822
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61370735143786	33.0	33.0	33.0	33.0	33.0
2	32.615925189791156	33.0	33.0	33.0	33.0	33.0
3	32.667168997020084	33.0	33.0	33.0	33.0	33.0
4	36.5802040406084	37.0	37.0	37.0	37.0	37.0
5	36.60397640200228	37.0	37.0	37.0	37.0	37.0
6	36.202701219713575	37.0	37.0	37.0	33.0	37.0
7	36.06811599592345	37.0	37.0	37.0	33.0	37.0
8	35.984311689959796	37.0	37.0	37.0	33.0	37.0
9	35.98201596656378	37.0	37.0	37.0	33.0	37.0
10-11	35.91941349052156	37.0	37.0	37.0	33.0	37.0
12-13	35.83279082521998	37.0	37.0	37.0	33.0	37.0
14-15	37.570841842393435	40.0	37.0	40.0	33.0	40.0
16-17	37.5104436170802	40.0	37.0	40.0	33.0	40.0
18-19	37.533425295761624	40.0	37.0	40.0	33.0	40.0
20-21	37.447082086778764	40.0	37.0	40.0	33.0	40.0
22-23	37.336701001869926	40.0	37.0	40.0	33.0	40.0
24-25	37.32562498749495	40.0	37.0	40.0	33.0	40.0
26-27	37.17242630229482	40.0	37.0	40.0	33.0	40.0
28-29	37.01927841184096	40.0	37.0	40.0	33.0	40.0
30-31	36.845348788989384	40.0	37.0	40.0	33.0	40.0
32-33	36.636319148630285	40.0	37.0	40.0	33.0	40.0
34-35	36.54031546118006	40.0	37.0	40.0	33.0	40.0
36-37	36.375538182323496	40.0	37.0	40.0	33.0	40.0
38-39	36.4025321193932	40.0	37.0	40.0	33.0	40.0
40-41	36.4267235107106	40.0	37.0	40.0	33.0	40.0
42-43	36.33522145691531	40.0	37.0	40.0	33.0	40.0
44-45	36.21340824551349	37.0	37.0	40.0	30.0	40.0
46-47	36.04650020629752	37.0	37.0	40.0	27.0	40.0
48-49	35.8499375330958	37.0	37.0	40.0	27.0	40.0
50-51	35.825137379907	37.0	37.0	40.0	27.0	40.0
52-53	35.70869205997886	37.0	37.0	40.0	27.0	40.0
54-55	35.51148601719936	37.0	33.0	40.0	27.0	40.0
56-57	35.273036839325144	37.0	33.0	40.0	27.0	40.0
58-59	35.14120565841522	37.0	33.0	40.0	27.0	40.0
60-61	35.005949726209764	37.0	33.0	40.0	27.0	40.0
62-63	34.83095914091983	37.0	33.0	40.0	27.0	40.0
64-65	34.634187156203836	37.0	33.0	40.0	27.0	40.0
66-67	34.364914188696304	37.0	33.0	37.0	27.0	40.0
68-69	34.14358991175089	37.0	33.0	37.0	27.0	40.0
70-71	33.91842876970016	37.0	33.0	37.0	27.0	40.0
72-73	33.63172252368927	37.0	33.0	37.0	27.0	40.0
74-75	33.29245389770257	37.0	33.0	37.0	24.5	38.5
76-77	31.13475259883984	33.0	30.0	35.0	22.0	37.0
78-79	33.25037760053847	35.0	33.0	37.0	27.0	37.0
80-81	33.624011168109604	37.0	33.0	37.0	27.0	37.0
82-83	33.586060872469204	37.0	33.0	37.0	27.0	37.0
84-85	33.497590918394515	37.0	33.0	37.0	27.0	37.0
86-87	33.37028178600758	37.0	33.0	37.0	27.0	37.0
88-89	33.21193250339492	35.0	33.0	37.0	27.0	37.0
90-91	33.13230060009023	33.0	33.0	37.0	27.0	37.0
92-93	33.1825761276169	33.0	33.0	37.0	27.0	37.0
94-95	33.23807199123921	33.0	33.0	37.0	27.0	37.0
96-97	33.34182541953547	33.0	33.0	37.0	27.0	37.0
98-99	33.47543667815422	33.0	33.0	37.0	27.0	37.0
100	33.451779154645706	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	10.0
16	39.0
17	89.0
18	295.0
19	873.0
20	2182.0
21	5683.0
22	14257.0
23	31534.0
24	59933.0
25	98496.0
26	142714.0
27	186924.0
28	225496.0
29	253846.0
30	274417.0
31	292832.0
32	316460.0
33	357833.0
34	430307.0
35	577544.0
36	945922.0
37	1944201.0
38	1498033.0
39	30900.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.738867410904504	19.32339863325385	12.71087963170642	24.226854324135225
2	15.53044704345298	21.16721078026254	40.03778022456973	23.26456195171475
3	18.698011541208672	26.232499740915095	29.883379933942127	25.18610878393411
4	12.812176507555249	17.206648062715452	37.996648064015766	31.98452736571354
5	13.442661063103667	38.250987768406	35.051693440791304	13.254657727699032
6	31.15547336812632	37.024039823051424	18.343617886358572	13.476868922463684
7	27.601015860203237	32.30762589486533	22.616581166486498	17.47477707844493
8	25.937773621597277	35.667110745769435	20.84943586004201	17.545679772591278
9	26.98009393534267	15.775088800650957	20.17411142788118	37.07070583612519
10-11	25.19634462619951	26.095976316681174	28.47701816738774	20.23066088973157
12-13	26.058923826138784	23.643483325139464	28.383182876897305	21.914409971824444
14-15	23.094123945828514	24.889515774335543	25.84995730164607	26.16640297818987
16-17	22.795976814962042	27.876344037087325	26.467742979879134	22.859936168071503
18-19	22.845457871733345	27.120235002188327	28.06909976592879	21.965207360149538
20-21	23.79746800862539	26.448503409360548	27.69743798418956	22.056590597824503
22-23	23.39943550785643	26.37447275367532	27.63410301190714	22.59198872656111
24-25	23.153307378389236	26.545511731830686	27.759092306460037	22.542088583320044
26-27	23.092111818917807	26.726289335478825	27.730150253618035	22.451448591985333
28-29	23.015614530616464	26.70024834380183	27.73499127179718	22.54914585378452
30-31	23.403279279298207	26.60534915887831	27.538671688058713	22.452699873764768
32-33	23.130008166917428	26.874152711143147	27.543978306769713	22.45186081516971
34-35	23.16405325241238	26.855555870853763	27.57515017069429	22.405240706039564
36-37	23.25864396510259	26.708417153198354	27.53014857707751	22.50279030462154
38-39	22.9911168291778	26.928572953841147	27.588504728526775	22.49180548845428
40-41	23.20111156923675	26.766245302815463	27.51951782835038	22.51312529959741
42-43	23.135950323372853	26.743144612782288	27.39008578476472	22.730819279080137
44-45	23.077405828075655	26.92504002411522	27.209389847060628	22.788164300748498
46-47	23.276587255248096	26.769001405946653	26.93144690534487	23.022964433460384
48-49	23.047449927235828	26.92317785307774	27.061308903801486	22.968063315884947
50-51	23.000356229448933	27.07919382162079	26.996748582796926	22.923701366133347
52-53	23.23666620649	27.017223525823347	26.764091444016923	22.98201882366973
54-55	23.166954169767564	26.88590023156098	26.84225272594773	23.10489287272372
56-57	23.10399273470675	27.111589299915707	26.78164327909322	23.00277468628432
58-59	23.011978291439796	27.184408843596415	26.865044506784567	22.93856835817922
60-61	23.117085076965246	27.1822929942651	26.825456192148287	22.875165736621366
62-63	23.037413446748484	27.328975584022686	26.738697527945142	22.894913441283688
64-65	23.012155603560355	27.272905918547398	26.76240574952637	22.952532728365878
66-67	23.10637263492483	27.28492087848146	26.608763957956604	22.999942528637103
68-69	22.964168975411127	27.248013088341093	26.72847859281915	23.05933934342863
70-71	22.990969963550956	26.951953866330296	26.832823775568805	23.224252394549943
72-73	23.08853581637953	27.023392418718284	26.670262481913248	23.217809282988938
74-75	22.878679164666863	27.217872578821623	26.662107711778237	23.241340544733276
76-77	23.02928222910324	27.057411509087103	26.676020509653142	23.23728575215651
78-79	22.990936495808324	27.134032231184392	26.603570432778646	23.27146084022864
80-81	23.049647303043212	27.106992835431676	26.573782702538974	23.269577158986138
82-83	23.075037429719977	26.98022598247042	26.78150478052408	23.163231807285523
84-85	23.140138393932595	27.087970693049567	26.655103380101515	23.116787532916323
86-87	22.913537540627516	27.104460839772106	26.760291528068418	23.22171009153196
88-89	22.988946944643146	27.05004198675085	26.755426312388337	23.205584756217668
90-91	23.039351751347873	27.04864650345436	26.84982695253506	23.062174792662706
92-93	23.09120579420181	26.998467153893614	26.83451863517936	23.075808416725216
94-95	22.920316108573992	27.04830199544447	26.918725134617468	23.112656761364075
96-97	22.95693444262347	27.08240361836686	27.075326827842183	22.885335111167485
98-99	24.117121435642147	29.02779967350796	27.225642228468377	19.62943666238151
100	23.225679368382632	29.767349926760446	20.995135229141315	26.01183547571561
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	865.0
1	864.5
2	1388.0
3	2119.5
4	3150.0
5	5791.0
6	8093.0
7	9055.5
8	9044.0
9	8092.0
10	7002.5
11	6126.5
12	6458.5
13	8974.0
14	14900.5
15	23412.5
16	31238.5
17	36396.0
18	38080.5
19	36055.5
20	32520.0
21	28997.0
22	26624.5
23	26791.5
24	29730.0
25	35921.5
26	45122.0
27	55991.0
28	67169.0
29	80204.0
30	93938.5
31	108243.0
32	125632.0
33	143415.5
34	161200.5
35	179128.0
36	199412.5
37	219308.0
38	233395.0
39	245334.5
40	260403.0
41	274268.5
42	283706.5
43	294674.0
44	305347.0
45	314996.0
46	323088.5
47	329165.0
48	336226.5
49	338458.5
50	336617.5
51	335222.5
52	329057.5
53	318697.5
54	306748.5
55	290384.0
56	272073.5
57	251958.0
58	229900.5
59	206359.0
60	178035.0
61	148682.5
62	121629.0
63	97283.5
64	76171.5
65	58934.5
66	45930.0
67	36439.0
68	28594.0
69	21268.5
70	15735.5
71	11665.5
72	8445.5
73	6069.5
74	4271.0
75	3138.0
76	2356.5
77	1730.5
78	1285.0
79	934.0
80	652.0
81	452.5
82	341.0
83	241.5
84	173.0
85	133.0
86	103.0
87	82.5
88	57.5
89	36.5
90	23.5
91	16.0
92	14.0
93	7.5
94	2.5
95	2.0
96	1.5
97	2.0
98	1.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.3002511305033456E-5
2	3.380652939308698E-4
3	0.0038097358123748023
4	0.004433856355016408
5	1.3002511305033456E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.0402009044026764E-4
12-13	0.0014692837774687804
14-15	0.0031791140140806796
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.006894831724072224
26-27	0.02151819482192323
28-29	0.009740334338285346
30-31	0.0
32-33	0.0
34-35	1.5213666688142192E-4
36-37	0.011963660497487665
38-39	0.033687575421811805
40-41	0.038132793263966056
42-43	0.006658838712008056
44-45	3.2566025751449174E-4
46-47	2.051079117163587E-5
48-49	0.03366219788194614
50-51	0.05887458680366507
52-53	0.05381658030842183
54-55	0.028080351275002587
56-57	0.0012408363350346026
58-59	0.006217883362382321
60-61	0.030540999306982005
62-63	0.03328773569100823
64-65	0.014445475504874152
66-67	0.03910399105853863
68-69	0.05573657872916225
70-71	0.07321548414797416
72-73	0.05218972480001135
74-75	0.022940194196847747
76-77	0.005128331978119747
78-79	3.5797272884202186E-4
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0022017869667652194
92-93	0.002765647968196318
94-95	6.06454844118713E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2119.0
20-21	6141.0
22-23	7061.0
24-25	12784.0
26-27	17165.0
28-29	24458.0
30-31	25952.0
32-33	27873.0
34-35	32216.0
36-37	32006.0
38-39	32835.0
40-41	40303.0
42-43	46686.0
44-45	54634.0
46-47	55227.0
48-49	44896.0
50-51	52117.0
52-53	48693.0
54-55	59067.0
56-57	62920.0
58-59	63283.0
60-61	63406.0
62-63	66613.0
64-65	78512.0
66-67	84795.0
68-69	75226.0
70-71	73882.0
72-73	65618.0
74-75	72603.0
76-77	56293.0
78-79	74548.0
80-81	85599.0
82-83	99938.0
84-85	107771.0
86-87	118161.0
88-89	129958.0
90-91	139345.0
92-93	149648.0
94-95	170905.0
96-97	324189.0
98-99	483585.0
100-101	4421791.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.06477170158293
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.68331439113767	78.04638750216132
2	6.409091646189682	11.031940186876746
3	1.626544328320857	4.199644988383173
4	0.5925253651061617	2.0398224110103547
5	0.2778962173752143	1.195853725256564
6	0.13852246951334282	0.7153142828523198
7	0.07775200343920048	0.4684195897734836
8	0.05015469231115485	0.34532417148181427
9	0.03780915497136502	0.2928632661756269
>10	0.10348311187572765	1.3688342735940942
>50	0.0024058111423949655	0.1465162215876242
>100	3.813812790995092E-4	0.04810358963628822
>500	7.165647372364583E-5	0.04236794765749871
>1k	4.7770864347846936E-5	0.05860784355306779
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.3002511305033454E-5	1.3002511305033454E-5
2	0.0	5.2010045220133815E-5	0.0	2.6005022610066908E-5	1.3002511305033454E-5
3	0.0	5.2010045220133815E-5	0.0	2.6005022610066908E-5	1.3002511305033454E-5
4	0.0	1.8203515827046836E-4	0.0	2.6005022610066908E-5	3.9007533915100365E-5
5	0.0	1.8203515827046836E-4	0.0	2.6005022610066908E-5	3.9007533915100365E-5
6	3.9007533915100365E-5	1.8203515827046836E-4	0.0	2.6005022610066908E-5	5.2010045220133815E-5
7	3.9007533915100365E-5	1.8203515827046836E-4	0.0	2.6005022610066908E-5	5.2010045220133815E-5
8	3.9007533915100365E-5	1.9503766957550182E-4	0.0	2.6005022610066908E-5	6.501255652516727E-5
9	3.9007533915100365E-5	3.120602713208029E-4	0.0	2.6005022610066908E-5	7.801506783020073E-5
10-11	3.9007533915100365E-5	3.120602713208029E-4	0.0	2.6005022610066908E-5	7.801506783020073E-5
12-13	3.9007533915100365E-5	3.120602713208029E-4	0.0	2.6005022610066908E-5	7.801506783020073E-5
14-15	3.9007533915100365E-5	4.940954295912713E-4	0.0	3.250627826258363E-5	1.4952888000788471E-4
16-17	6.501255652516727E-5	5.070979408963047E-4	0.0	3.9007533915100365E-5	1.690326469654349E-4
18-19	6.501255652516727E-5	5.201004522013381E-4	0.0	3.9007533915100365E-5	1.690326469654349E-4
20-21	7.1513812177684E-5	5.461054748114051E-4	0.0	7.1513812177684E-5	1.690326469654349E-4
22-23	7.801506783020073E-5	6.241205426416058E-4	0.0	2.210426921855687E-4	1.7553390261795164E-4
24-25	7.801506783020073E-5	7.476444000394237E-4	0.0	6.176192869890891E-4	1.9503766957550182E-4
26-27	7.801506783020073E-5	8.32160723522141E-4	0.0	0.0012677448522407618	1.9503766957550182E-4
28-29	7.801506783020073E-5	8.32160723522141E-4	0.0	0.005396042191588884	1.9503766957550182E-4
30-31	7.801506783020073E-5	0.001001193370487576	0.0	0.00935530688397157	2.0153892522801854E-4
32-33	7.801506783020073E-5	0.0010076946261400929	0.0	0.014543308894679918	2.0804018088053526E-4
34-35	7.801506783020073E-5	0.001215734807020628	0.0	0.02139563235243255	2.0804018088053526E-4
36-37	7.801506783020073E-5	0.0014172737322486464	0.0	0.032265731803440514	2.2104269218556873E-4
38-39	7.801506783020073E-5	0.0014432787548587134	0.0	0.054935610263766345	2.2104269218556873E-4
40-41	7.801506783020073E-5	0.0016123114018241483	0.0	0.08092763036252823	2.2104269218556873E-4
42-43	7.801506783020073E-5	0.001618812657476665	0.0	0.10459870219334162	2.2754394783808544E-4
44-45	7.801506783020073E-5	0.0017943465600946166	0.0	0.13324323459833032	2.3404520349060216E-4
46-47	7.801506783020073E-5	0.001950376695755018	0.0	0.16641914219312318	2.3404520349060216E-4
48-49	7.801506783020073E-5	0.001950376695755018	0.0	0.19858735516177595	2.470477147956356E-4
50-51	8.451632348271745E-5	0.0021779206435931036	0.0	0.2299754174521267	2.470477147956356E-4
52-53	1.0402009044026763E-4	0.0021844218992456204	0.0	0.2855936595594073	2.86055248710736E-4
54-55	1.0402009044026763E-4	0.0022039256662031706	0.0	0.35492304983784567	3.4456654958338654E-4
56-57	1.0402009044026763E-4	0.0022234294331607207	0.0	0.4316898765827632	3.510678052359033E-4
58-59	1.0402009044026763E-4	0.002236431944465754	0.0	0.492632647069455	3.705715721934534E-4
60-61	1.0402009044026763E-4	0.0022559357114233045	0.0	0.5380439178022843	3.9007533915100365E-4
62-63	1.3002511305033453E-4	0.002301444500990921	0.0	0.5784752267052858	4.030778504560371E-4
64-65	1.3002511305033453E-4	0.002450973380998806	0.0	0.5917637932590301	4.030778504560371E-4
66-67	1.3002511305033453E-4	0.0024704771479563564	0.0	0.6001699168177341	4.030778504560371E-4
68-69	1.3002511305033453E-4	0.0025354897044815236	0.0	0.6080429374129319	4.030778504560371E-4
70-71	1.43027624355368E-4	0.0025549934714390737	0.0	0.6136275160184438	4.030778504560371E-4
72-73	1.690326469654349E-4	0.0025614947270915906	0.0	0.619719192564852	4.810929182862378E-4
74-75	1.690326469654349E-4	0.002646011050574308	0.0	0.6251997510799236	4.810929182862378E-4
76-77	1.8203515827046836E-4	0.002737028629709542	0.0	0.6296661137132026	4.875941739387545E-4
78-79	1.8203515827046836E-4	0.0027695349079721256	0.0	0.632201603417684	4.940954295912713E-4
80-81	1.8203515827046836E-4	0.0027695349079721256	0.0	0.6331637892542565	5.00596685243788E-4
82-83	1.8203515827046836E-4	0.0027695349079721256	0.0	0.6351856797621893	5.070979408963047E-4
84-85	1.8853641392298508E-4	0.0027695349079721256	0.0	0.6369085125101062	5.070979408963047E-4
86-87	1.9503766957550182E-4	0.0027695349079721256	0.0	0.6378186883014585	5.201004522013381E-4
88	2.0804018088053526E-4	0.002886557509717427	0.0	0.6378771996023312	5.331029635063716E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8375	0.0	47.72681	1
GTATCAA	15215	0.0	37.154984	1
TCAACGC	18235	0.0	30.101631	4
ATCAACG	18755	0.0	29.380037	3
CAACGCA	18885	0.0	28.998236	5
TATCAAC	19325	0.0	28.9267	2
AACGCAG	19500	0.0	28.213728	6
GTACATG	26595	0.0	27.451529	1
TACATGG	26095	0.0	27.397676	2
ACATGGG	26430	0.0	26.446112	3
CATGGGG	20790	0.0	23.996552	4
ACGCAGA	22760	0.0	23.950972	7
CGCAGAG	23525	0.0	23.19074	8
GCAGAGT	27545	0.0	19.791084	9
ATGGGGG	12155	0.0	19.501513	5
GTGGTAT	4085	0.0	19.446278	1
GAGTACT	14465	0.0	18.044292	12-13
AGAGTAC	24070	0.0	17.872452	10-11
CAGAGTA	26420	0.0	17.467949	10-11
GTATAAT	5020	0.0	17.415113	1
>>END_MODULE