FastQCFastQC Report
Fri 27 May 2016
SRR2139348_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139348_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8163950
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87000.047.2078171
GTATCAA155750.034.475751
TCAACGC195200.026.7090364
ATCAACG197250.026.4518553
CAACGCA199850.025.9699655
TATCAAC203650.025.9649812
AACGCAG207150.025.0930146
GTACATG250500.023.2399621
TACATGG247400.023.0114022
ACATGGG257100.021.764313
ACGCAGA240800.021.473187
CGCAGAG245600.021.0700198
CATGGGG197600.019.7786184
GCAGAGT288100.017.6921659
GTGGTAT41350.017.3394341
GTATAAT46500.017.1788251
GAGTACT155250.017.11843112-13
ATGGGGG118750.016.7999365
AGAGTAC253350.016.46577810-11
CAGAGTA276450.016.30217610-11
Produced by FastQC (version 0.11.5)