##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139348_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8163950
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.446860037114384	33.0	33.0	33.0	33.0	33.0
2	32.453820270824785	33.0	33.0	33.0	33.0	33.0
3	32.523329270757415	33.0	33.0	33.0	33.0	33.0
4	36.40345408778839	37.0	37.0	37.0	37.0	37.0
5	36.42923401049737	37.0	37.0	37.0	37.0	37.0
6	35.89386522455429	37.0	37.0	37.0	33.0	37.0
7	35.71959100680431	37.0	37.0	37.0	33.0	37.0
8	35.60910956093558	37.0	37.0	37.0	33.0	37.0
9	35.61690652196547	37.0	37.0	37.0	33.0	37.0
10-11	35.53036244709975	37.0	37.0	37.0	33.0	37.0
12-13	35.41808119843948	37.0	37.0	37.0	33.0	37.0
14-15	36.80472032533271	37.0	37.0	40.0	33.0	40.0
16-17	36.716102376913135	37.0	37.0	40.0	33.0	40.0
18-19	36.73624434250577	37.0	37.0	40.0	33.0	40.0
20-21	36.62299936550838	37.0	37.0	40.0	33.0	40.0
22-23	36.46990708917944	37.0	37.0	40.0	33.0	40.0
24-25	36.45692543324644	37.0	37.0	40.0	33.0	40.0
26-27	36.262547997924955	37.0	37.0	40.0	33.0	40.0
28-29	36.05298941495886	37.0	37.0	40.0	27.0	40.0
30-31	35.820792203659636	37.0	37.0	40.0	27.0	40.0
32-33	35.54711991537115	37.0	33.0	40.0	27.0	40.0
34-35	35.41584376362181	37.0	33.0	40.0	27.0	40.0
36-37	35.17090880614957	37.0	33.0	40.0	27.0	40.0
38-39	35.20164535779469	37.0	33.0	40.0	27.0	40.0
40-41	35.24885578466764	37.0	33.0	40.0	27.0	40.0
42-43	35.13066037669846	37.0	33.0	40.0	27.0	40.0
44-45	34.95943571725858	37.0	33.0	40.0	27.0	40.0
46-47	34.72811139835575	37.0	33.0	40.0	24.5	40.0
48-49	34.47694485900974	37.0	33.0	40.0	22.0	40.0
50-51	34.46630904949241	37.0	33.0	40.0	22.0	40.0
52-53	34.30637789651081	37.0	33.0	40.0	22.0	40.0
54-55	34.059539574040585	37.0	33.0	40.0	22.0	40.0
56-57	33.73879676000875	37.0	33.0	40.0	22.0	40.0
58-59	33.560180423176746	37.0	33.0	40.0	22.0	40.0
60-61	33.406349295661	37.0	33.0	37.0	22.0	40.0
62-63	33.203054390422565	37.0	33.0	37.0	22.0	40.0
64-65	33.01114791741847	37.0	33.0	37.0	22.0	40.0
66-67	32.71784862277336	37.0	33.0	37.0	22.0	40.0
68-69	32.502237475490645	37.0	33.0	37.0	22.0	40.0
70-71	32.23102085176838	35.0	30.0	37.0	22.0	40.0
72-73	31.87087840318692	33.0	27.0	37.0	22.0	37.0
74-75	31.452205422701752	33.0	27.0	37.0	22.0	37.0
76-77	29.64084197282923	33.0	27.0	35.0	18.5	37.0
78-79	31.79314152337168	33.0	30.0	37.0	22.0	37.0
80-81	32.20915543136894	33.0	33.0	37.0	22.0	37.0
82-83	32.13249728593586	33.0	33.0	37.0	22.0	37.0
84-85	31.994951181990004	33.0	33.0	37.0	22.0	37.0
86-87	31.828454901168527	33.0	33.0	37.0	22.0	37.0
88-89	31.646009146637507	33.0	33.0	37.0	22.0	37.0
90-91	31.571506012211007	33.0	27.0	37.0	22.0	37.0
92-93	31.64037437768964	33.0	33.0	37.0	22.0	37.0
94-95	31.727219047608852	33.0	33.0	37.0	22.0	37.0
96-97	31.932830359749794	33.0	33.0	37.0	27.0	37.0
98-99	32.19988652042614	33.0	33.0	37.0	27.0	37.0
100	32.15043013152678	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	2.0
15	10.0
16	53.0
17	136.0
18	452.0
19	1141.0
20	3129.0
21	8119.0
22	20548.0
23	45214.0
24	85599.0
25	140199.0
26	203873.0
27	267391.0
28	322227.0
29	362889.0
30	393042.0
31	417817.0
32	447165.0
33	497667.0
34	584513.0
35	750081.0
36	1085258.0
37	1590764.0
38	916953.0
39	19706.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.45267303204944	20.23721360370899	13.33687736941064	23.973235994830933
2	15.643665210748075	21.712601718929562	39.83940064072177	22.804332429600592
3	18.49280502043914	26.564918466371086	30.283467838546706	24.65880867464307
4	12.905925774307653	17.921153407301546	38.136796240269675	31.036124578121132
5	13.435388544291138	38.373962986285925	35.20897428657976	12.981674182843175
6	30.759620036869407	37.522780026825245	18.399426748081503	13.31817318822384
7	27.15801787125105	32.47593383104992	22.861347754457096	17.504700543241935
8	25.60510537178694	36.00330722260671	21.008617152236354	17.382970253369997
9	26.77344912695448	16.004630111649384	20.446352562178845	36.77556819921729
10-11	25.06392134190109	26.372799163149164	28.64772401561011	19.915555479339634
12-13	25.877212806856818	23.892765155565744	28.396933137492752	21.83308890008468
14-15	22.96410387509549	25.170588832269058	25.90365028344655	25.961657009188897
16-17	22.723056853606405	28.081823137084378	26.58957367450805	22.60554633480117
18-19	22.785581734332034	27.30642642348373	28.09549299052542	21.81249885165882
20-21	23.644145336900742	26.588140518802284	27.739498388053637	22.028215756243338
22-23	23.30518174263597	26.64123356668681	27.602096132996024	22.451488557681195
24-25	23.060791739371677	26.768226713957095	27.80357555396627	22.36740599270496
26-27	23.01844774217183	26.947883011915224	27.740601810449256	22.293067435463687
28-29	22.87047349716801	26.93849929609526	27.694083432015898	22.496943774720833
30-31	23.207059423634142	26.80805593314658	27.60652139880501	22.378363244414263
32-33	22.95760470044204	27.06219692682484	27.56215763516161	22.41804073757151
34-35	23.080908773047568	27.135731121564184	27.616625176563304	22.166734928824948
36-37	23.137883330538557	27.049514011932015	27.425123138527308	22.387479519002124
38-39	22.91282402317378	27.137180629236408	27.594384219279394	22.355611128310414
40-41	23.115501326227193	27.016761232413067	27.51885087150511	22.348886569854635
42-43	23.05843324496291	26.986304567014496	27.414205176843893	22.5410570111787
44-45	22.989520115666267	27.186828524970576	27.24674425172588	22.576907107637282
46-47	23.20032441720995	26.999527337392827	26.91023770622481	22.889910539172416
48-49	22.990699850695766	27.11575884732135	27.06674208820627	22.826799213776617
50-51	22.88258954579576	27.251138522714896	27.061428939780452	22.80484299170889
52-53	23.050023934830193	27.19346720257585	26.90938605833168	22.84712280426227
54-55	22.99460808934636	27.088601282858672	26.924257903084154	22.992532724710816
56-57	22.981942481498674	27.27606574377463	26.79048529342685	22.951506481299845
58-59	22.809921658112074	27.34238501025688	27.02528967808444	22.822403653546605
60-61	23.001297594850342	27.42568969890084	26.852027153340646	22.72098555290817
62-63	22.797196442850563	27.59499412391868	26.80754622113451	22.800263212096247
64-65	22.772595437013262	27.58967332250311	26.806462025129584	22.831269215354045
66-67	22.907394425792972	27.623365342768437	26.592092428138898	22.8771478032997
68-69	22.797972082555017	27.551124516020604	26.667236510997405	22.983666890426967
70-71	22.796415839644656	27.293243950261047	26.71154632704118	23.198793883053117
72-73	22.862222850054874	27.29212802184679	26.60734038651989	23.238308741578447
74-75	22.747715127741785	27.419465651363232	26.573128159470073	23.25969106142491
76-77	22.872651455176793	27.273184109889208	26.58343283595088	23.27073159898311
78-79	22.830849886118003	27.32182370805542	26.56116181765781	23.286164588168766
80-81	22.823683075089207	27.313205711224224	26.546504737799935	23.316606475886633
82-83	22.879104060572743	27.227159074115516	26.685737550175663	23.207999315136078
84-85	22.935840251021617	27.360811547577335	26.602411607103306	23.100936594297742
86-87	22.714917408459506	27.309552040432482	26.776579630648413	23.198950920459595
88-89	22.700386338347506	27.24209791113852	26.868382741250063	23.189133009263905
90-91	22.68495199911352	27.18636776502903	27.019155862689594	23.109524373167854
92-93	22.663642648308127	27.239049650941897	27.025287831940965	23.072019868809008
94-95	22.514251504655817	27.326431996759986	27.10291096057191	23.05640553801228
96-97	22.458027348015854	27.39828636287143	27.317509819577566	22.82617646953514
98-99	23.636330444288614	29.43477850000913	27.434819306736653	19.494071748965602
100	22.808475137405118	30.272561034036993	21.068469319311767	25.85049450924612
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1228.0
1	1156.5
2	1650.0
3	2439.5
4	3477.0
5	6128.5
6	8484.5
7	9436.5
8	9455.0
9	8698.5
10	7757.5
11	6959.5
12	7398.0
13	10006.0
14	15914.0
15	24806.5
16	33232.5
17	38795.5
18	41335.5
19	40671.5
20	38472.0
21	35912.5
22	34429.5
23	35641.0
24	39392.0
25	46582.5
26	56880.5
27	68553.0
28	81203.0
29	95881.5
30	111257.0
31	127251.5
32	145562.5
33	164666.0
34	185478.5
35	207364.0
36	230998.5
37	253678.0
38	273297.5
39	292355.0
40	312803.0
41	331773.0
42	345708.5
43	359777.5
44	374119.5
45	386403.0
46	397592.0
47	407754.0
48	411930.0
49	408978.0
50	405238.0
51	398832.0
52	385142.5
53	366574.0
54	346626.5
55	325267.5
56	301775.0
57	276051.5
58	248442.5
59	218999.0
60	187931.5
61	157928.0
62	130800.5
63	106352.0
64	84270.0
65	66058.0
66	51739.0
67	41238.0
68	33051.0
69	25558.5
70	19397.5
71	14695.5
72	11217.5
73	8457.0
74	6225.5
75	4619.5
76	3456.5
77	2541.5
78	1876.0
79	1365.0
80	985.0
81	748.5
82	588.0
83	425.0
84	297.0
85	206.5
86	153.0
87	120.5
88	85.0
89	57.5
90	40.0
91	27.5
92	19.5
93	8.5
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	3.919671237574949E-4
3	0.002474292468719186
4	0.0021190722628139564
5	3.6746917852265136E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.2248972617421713E-4
12-13	0.002045578427109426
14-15	0.002958126887107344
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.00519020345106148
26-27	0.01678716655282337
28-29	0.007202227427811568
30-31	0.0
32-33	0.0
34-35	2.6326933882538247E-4
36-37	0.015774950603068345
38-39	0.044179961662742774
40-41	0.05053200020390218
42-43	0.009081311869281114
44-45	3.62246128856744E-4
46-47	6.687359586346685E-6
48-49	0.03140295323498266
50-51	0.04300869435285377
52-53	0.048607521254530724
54-55	0.026696368323774686
56-57	5.467084303950609E-4
58-59	0.0059371871917616404
60-61	0.028256897606913075
62-63	0.03761245605856128
64-65	0.02157304908805118
66-67	0.05675493867053621
68-69	0.07143119765986965
70-71	0.0759111613997851
72-73	0.047552994822369694
74-75	0.019088343447510127
76-77	0.0037883587654130683
78-79	2.3106052426033306E-4
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0013221475453701226
92-93	0.001988493036308253
94-95	5.32297972250514E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2658.0
20-21	7748.0
22-23	9440.0
24-25	16633.0
26-27	22601.0
28-29	33845.0
30-31	37588.0
32-33	43793.0
34-35	53084.0
36-37	65043.0
38-39	72827.0
40-41	84344.0
42-43	95418.0
44-45	111331.0
46-47	114604.0
48-49	102777.0
50-51	105930.0
52-53	97133.0
54-55	108283.0
56-57	111715.0
58-59	116514.0
60-61	116068.0
62-63	116502.0
64-65	126857.0
66-67	130551.0
68-69	128652.0
70-71	125079.0
72-73	117247.0
74-75	121131.0
76-77	110982.0
78-79	128676.0
80-81	138748.0
82-83	139578.0
84-85	143695.0
86-87	149813.0
88-89	151903.0
90-91	160925.0
92-93	178024.0
94-95	210645.0
96-97	337911.0
98-99	431976.0
100-101	3485678.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.30957990117551
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.9018671863806	82.97026730450453
2	5.016881033330761	8.961110750018912
3	1.16880627180678	3.1315679136276806
4	0.4155621295280596	1.4845471684395557
5	0.18926437591218923	0.8451560951487892
6	0.10516141159851661	0.5635152895006876
7	0.06643202850217798	0.4153111590258704
8	0.03346258400283046	0.2390823455760468
9	0.024161239770560585	0.19420471564203062
>10	0.07671230934402208	1.0658330751288514
>50	0.0014300113455027177	0.08820424946934498
>100	2.3985837529024776E-4	0.028884256814102904
>500	1.9560102685868488E-5	0.012315677103576325
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	3.674691785226514E-5	0.0	0.0	0.0
5	0.0	4.899589046968685E-5	0.0	1.2248972617421713E-5	0.0
6	0.0	4.899589046968685E-5	0.0	1.2248972617421713E-5	0.0
7	0.0	6.124486308710856E-5	0.0	1.2248972617421713E-5	0.0
8	0.0	6.124486308710856E-5	0.0	1.2248972617421713E-5	0.0
9	0.0	1.3473869879163886E-4	0.0	2.4497945234843426E-5	0.0
10-11	0.0	1.5923664402648228E-4	0.0	7.349383570453029E-5	0.0
12-13	0.0	1.5923664402648228E-4	0.0	8.5742808321952E-5	0.0
14-15	0.0	2.2048150711359083E-4	0.0	9.186729463066285E-5	3.062243154355428E-5
16-17	0.0	2.511039386571451E-4	0.0	9.79917809393737E-5	8.5742808321952E-5
18-19	0.0	2.7560188389198857E-4	0.0	1.2248972617421715E-4	9.79917809393737E-5
20-21	0.0	2.9397534281812114E-4	0.0	1.71485616643904E-4	1.0411626724808457E-4
22-23	0.0	3.490957195965188E-4	0.0	2.817263702006994E-4	1.3473869879163886E-4
24-25	0.0	3.919671237574948E-4	0.0	7.778097612062787E-4	1.5923664402648228E-4
26-27	0.0	4.1646506899233824E-4	0.0	0.001580117467647401	1.5923664402648228E-4
28-29	0.0	4.348385279184708E-4	0.0	0.005285431684417469	1.5923664402648228E-4
30-31	0.0	5.450792814752662E-4	0.0	0.00930309470293179	1.5923664402648228E-4
32-33	0.0	5.695772267101096E-4	0.0	0.01829996509042804	1.71485616643904E-4
34-35	0.0	7.226893844278811E-4	0.0	0.03094702931791596	1.837345892613257E-4
36-37	0.0	8.941750010717851E-4	0.0	0.052345984480551694	1.959835618787474E-4
38-39	0.0	9.676688367763154E-4	0.0	0.10230341930070616	1.959835618787474E-4
40-41	0.0	0.001028913699863424	0.0	0.16305219899680914	1.959835618787474E-4
42-43	0.0	0.0010534116450982675	0.0	0.2135179661805866	1.959835618787474E-4
44-45	0.0	0.001151403426037641	0.0	0.2782047905731907	1.959835618787474E-4
46-47	0.0	0.0012555196932857258	0.0	0.34639482113437736	2.0210804818745825E-4
48-49	0.0	0.0012677686659031474	0.0	0.4146767189901947	2.0823253449616912E-4
50-51	6.124486308710857E-6	0.0013902583920773646	0.0	0.4803312122195751	2.0823253449616912E-4
52-53	1.2248972617421713E-5	0.001408631851003497	0.0	0.5915763815309991	2.1435702080488E-4
54-55	1.2248972617421713E-5	0.001457627741473184	0.0	0.7233998248396916	2.2048150711359086E-4
56-57	1.2248972617421713E-5	0.0015127481182515817	0.0	0.8644773669608461	2.2048150711359086E-4
58-59	1.837345892613257E-5	0.0015494950361038468	0.0	0.9916400761886097	2.2660599342230173E-4
60-61	2.4497945234843426E-5	0.0015984909265735338	0.0	1.0500615510874025	2.4497945234843425E-4
62-63	2.4497945234843426E-5	0.0016229888718083771	0.0	1.1194519809650965	2.694773975832777E-4
64-65	2.4497945234843426E-5	0.0017883500021435702	0.0	1.1458913883598014	2.694773975832777E-4
66-67	2.4497945234843426E-5	0.001806723461069703	0.0	1.155978417310248	2.694773975832777E-4
68-69	2.4497945234843426E-5	0.0018495948652306787	0.0	1.1650304080745228	2.694773975832777E-4
70-71	2.4497945234843426E-5	0.0018679683241568114	0.0	1.1725084058574589	2.7560188389198857E-4
72-73	2.4497945234843426E-5	0.0018740928104655221	0.0	1.1809479479908622	3.062243154355428E-4
74-75	2.4497945234843426E-5	0.001886341783082944	0.0	1.187605264608431	3.062243154355428E-4
76-77	2.4497945234843426E-5	0.0019414621598613415	0.0	1.1939012365337858	3.123488017442537E-4
78-79	2.4497945234843426E-5	0.0019843335640223176	0.0	1.1978760281481389	3.1847328805296456E-4
80-81	2.4497945234843426E-5	0.002008831509257161	0.0	1.1994010252390082	3.245977743616754E-4
82-83	2.4497945234843426E-5	0.002014955995565872	0.0	1.2029716007569866	3.3072226067038625E-4
84-85	2.4497945234843426E-5	0.002039453940800715	0.0	1.2055500094929537	3.3072226067038625E-4
86-87	2.4497945234843426E-5	0.0020700763723442697	0.0	1.2069708903165748	3.490957195965189E-4
88	2.4497945234843426E-5	0.0021190722628139564	0.0	1.2071240024742924	3.552202059052297E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8700	0.0	47.207817	1
GTATCAA	15575	0.0	34.47575	1
TCAACGC	19520	0.0	26.709036	4
ATCAACG	19725	0.0	26.451855	3
CAACGCA	19985	0.0	25.969965	5
TATCAAC	20365	0.0	25.964981	2
AACGCAG	20715	0.0	25.093014	6
GTACATG	25050	0.0	23.239962	1
TACATGG	24740	0.0	23.011402	2
ACATGGG	25710	0.0	21.76431	3
ACGCAGA	24080	0.0	21.47318	7
CGCAGAG	24560	0.0	21.070019	8
CATGGGG	19760	0.0	19.778618	4
GCAGAGT	28810	0.0	17.692165	9
GTGGTAT	4135	0.0	17.339434	1
GTATAAT	4650	0.0	17.178825	1
GAGTACT	15525	0.0	17.118431	12-13
ATGGGGG	11875	0.0	16.799936	5
AGAGTAC	25335	0.0	16.465778	10-11
CAGAGTA	27645	0.0	16.302176	10-11
>>END_MODULE