FastQCFastQC Report
Fri 27 May 2016
SRR2139347_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139347_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9120091
Sequences flagged as poor quality0
Sequence length19-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106900.049.8791241
GTATCAA200800.037.7526781
CTTATAC79000.032.5086671
TATACAC92800.029.9553363
TCAACGC254600.029.1624764
TATCAAC260250.028.6836132
ATCAACG258900.028.6324713
CAACGCA262100.028.1902285
AACGCAG269100.027.561876
TTATACA105600.025.4924322
GTACATG369350.025.0663221
TACATGG365900.025.007942
ACATGGG373550.023.9931353
ACGCAGA316850.023.2947887
TACACAT121300.022.8174085
CGCAGAG326900.022.6403398
CATGGGG288100.021.9591374
GTATAAT60600.021.6180481
ACACATC123600.021.3919336
ACATCTC128400.020.872768
Produced by FastQC (version 0.11.5)