##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139347_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9120091
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.527071933821716	33.0	33.0	33.0	33.0	33.0
2	32.52886884571656	33.0	33.0	33.0	33.0	33.0
3	32.595932869529484	33.0	33.0	33.0	33.0	33.0
4	36.4896417151978	37.0	37.0	37.0	37.0	37.0
5	36.51656885879757	37.0	37.0	37.0	37.0	37.0
6	36.012263254829364	37.0	37.0	37.0	33.0	37.0
7	35.8418345825716	37.0	37.0	37.0	33.0	37.0
8	35.73591042019208	37.0	37.0	37.0	33.0	37.0
9	35.73127000596814	37.0	37.0	37.0	33.0	37.0
10-11	35.65426693659087	37.0	37.0	37.0	33.0	37.0
12-13	35.547148762002486	37.0	37.0	37.0	33.0	37.0
14-15	37.059419692193856	40.0	37.0	40.0	33.0	40.0
16-17	36.98185089381235	40.0	37.0	40.0	33.0	40.0
18-19	37.00158934817646	40.0	37.0	40.0	33.0	40.0
20-21	36.89488892412561	40.0	37.0	40.0	33.0	40.0
22-23	36.7499402181341	37.0	37.0	40.0	33.0	40.0
24-25	36.734081094279105	37.0	37.0	40.0	33.0	40.0
26-27	36.554186411233204	37.0	37.0	40.0	33.0	40.0
28-29	36.3556816554363	37.0	37.0	40.0	33.0	40.0
30-31	36.14499147337713	37.0	37.0	40.0	30.0	40.0
32-33	35.89576468259418	37.0	37.0	40.0	27.0	40.0
34-35	35.775687950538725	37.0	37.0	40.0	27.0	40.0
36-37	35.53975869483043	37.0	37.0	40.0	27.0	40.0
38-39	35.570727735906615	37.0	37.0	40.0	27.0	40.0
40-41	35.62982917172713	37.0	37.0	40.0	27.0	40.0
42-43	35.526059513791985	37.0	33.0	40.0	27.0	40.0
44-45	35.3638814502299	37.0	33.0	40.0	27.0	40.0
46-47	35.155551846144924	37.0	33.0	40.0	27.0	40.0
48-49	34.92307362417681	37.0	33.0	40.0	27.0	40.0
50-51	34.91005847324812	37.0	33.0	40.0	27.0	40.0
52-53	34.769568229013956	37.0	33.0	40.0	27.0	40.0
54-55	34.53961383619175	37.0	33.0	40.0	27.0	40.0
56-57	34.24989479411565	37.0	33.0	40.0	22.0	40.0
58-59	34.085433358475356	37.0	33.0	40.0	22.0	40.0
60-61	33.93242959742709	37.0	33.0	40.0	22.0	40.0
62-63	33.737264349965585	37.0	33.0	37.0	22.0	40.0
64-65	33.54781571742252	37.0	33.0	37.0	22.0	40.0
66-67	33.26142063205237	37.0	33.0	37.0	22.0	40.0
68-69	33.04444435086121	37.0	33.0	37.0	22.0	40.0
70-71	32.79163895773657	37.0	33.0	37.0	22.0	40.0
72-73	32.462184262688304	37.0	33.0	37.0	22.0	37.0
74-75	32.07547312802291	33.0	33.0	37.0	22.0	37.0
76-77	30.118978916001716	33.0	27.0	35.0	22.0	37.0
78-79	32.284864869045364	33.0	33.0	37.0	24.5	37.0
80-81	32.701997429046216	33.0	33.0	37.0	27.0	37.0
82-83	32.649771063108275	33.0	33.0	37.0	27.0	37.0
84-85	32.54138633729869	33.0	33.0	37.0	27.0	37.0
86-87	32.394509968875994	33.0	33.0	37.0	27.0	37.0
88-89	32.221669517357185	33.0	33.0	37.0	27.0	37.0
90-91	32.13676856211542	33.0	33.0	37.0	24.5	37.0
92-93	32.198225976383036	33.0	33.0	37.0	27.0	37.0
94-95	32.27325183389695	33.0	33.0	37.0	27.0	37.0
96-97	32.44193820941996	33.0	33.0	37.0	27.0	37.0
98-99	32.670362036469314	33.0	33.0	37.0	27.0	37.0
100	32.677741825153994	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	2.0
15	13.0
16	55.0
17	147.0
18	402.0
19	1305.0
20	3363.0
21	8479.0
22	21175.0
23	46528.0
24	88540.0
25	146182.0
26	211463.0
27	278222.0
28	335984.0
29	378466.0
30	408961.0
31	431766.0
32	460479.0
33	509632.0
34	599701.0
35	779463.0
36	1184002.0
37	1967778.0
38	1224346.0
39	33636.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.35294461425878	19.509575068932975	13.147544251477317	23.989936065330927
2	15.711957873873594	21.15160447195358	39.94601559357025	23.19042206060258
3	18.85298713966974	26.446079904138543	29.728400579484614	24.972532376707104
4	13.037559495215437	17.436787070315074	37.422179375518304	32.10347405895118
5	13.688394117997287	38.435219560857455	34.55871218828847	13.317674132856789
6	31.372329508554248	36.651158414976344	18.223480445535028	13.753031630934384
7	27.891026525941463	32.168056217860105	22.17280507398446	17.768112182213972
8	26.48816771674756	35.37374791545391	20.473765009581594	17.66431935821693
9	27.399441518730463	15.76375718180882	19.79118410112355	37.04561719833717
10-11	25.77433813895691	26.057060470638465	27.83995072849698	20.328650661907645
12-13	26.57066396547249	23.467768401839624	27.76378885904334	22.19777877364454
14-15	23.64905321098926	24.57583355597166	25.291156378071296	26.483956854967794
16-17	23.284175563599092	27.610585245256875	25.884242821699914	23.220996369444123
18-19	23.39925665215402	26.702880486609182	27.55757042336529	22.340292437871508
20-21	24.440015915494495	25.96786388905849	27.094620774613677	22.497499420833332
22-23	24.02277583151038	25.986614429723392	27.073567370127193	22.91704236863903
24-25	23.733590795170993	26.230226320394795	27.323594001527834	22.712588882906378
26-27	23.68839495152131	26.38889838363207	27.295369478681263	22.627337186165352
28-29	23.45436808508343	26.299989793838304	27.343861629893603	22.90178049118467
30-31	23.944435085799338	26.261263490403614	27.03520955561543	22.75909186818162
32-33	23.727299314902414	26.418291035417145	27.064244650259006	22.79016499942143
34-35	23.563947416374866	26.5455302925906	27.305090613913663	22.58543167712088
36-37	23.854233692861797	26.394800424513186	26.976502678150897	22.774463204474117
38-39	23.44477958862985	26.53807170064915	27.207777870917237	22.809370839803762
40-41	23.641832454755423	26.356821994385786	27.147175665844408	22.85416988501438
42-43	23.64885607777209	26.337076924721487	26.97117522266968	23.042891774836743
44-45	23.507007976270156	26.64141742270249	26.819121440539583	23.03245316048777
46-47	23.863467914452574	26.34739928677957	26.373882965554717	23.415249833213135
48-49	23.587795305838032	26.462340898765348	26.554217851554796	23.39564594384182
50-51	23.384330277460087	26.67770253329381	26.601030451925283	23.33693673732082
52-53	23.64931023781378	26.64481905579889	26.469733251844772	23.23613745454255
54-55	23.60052437295019	26.51674602369573	26.374552249344163	23.508177354009916
56-57	23.750951413816377	26.867112973306707	26.110810695977882	23.271124916899037
58-59	23.502026181257627	26.92055960520226	26.464935159832308	23.112479053707798
60-61	23.711229943744236	27.034521503716245	26.1560320297092	23.098216522830317
62-63	23.535256294755698	27.314578130026344	26.07876673225787	23.071398842960086
64-65	23.483315096453204	27.211822802623374	26.14318716905889	23.161674931864525
66-67	23.701533204156018	27.06067269213921	26.018000626098065	23.2197934776067
68-69	23.47901246191296	26.999018085924103	26.24205752284633	23.279911929316608
70-71	23.544578012817517	26.62875216579027	26.37263158592127	23.454038235470943
72-73	23.61629385204266	26.77990236620062	26.19277835459542	23.411025427161306
74-75	23.257722667676966	26.990382674973308	26.285128183333274	23.466766474016453
76-77	23.487379078476007	26.863044716027513	26.293287998201997	23.356288207294483
78-79	23.340752862929907	26.950310896660834	26.23152756454597	23.477408675863284
80-81	23.452156609094267	26.888188452335065	26.233705468594575	23.425949469976086
82-83	23.4314234280292	26.75387416118655	26.456883031984326	23.357819378799928
84-85	23.497723632095035	26.813481433602394	26.284819350120436	23.40397558418213
86-87	23.17887378396941	26.88946093606099	26.445998566184937	23.48566671378466
88-89	23.31592112927342	26.784574585772674	26.48448548855645	23.41501879639745
90-91	23.316847721084272	26.8603489314005	26.588151933876063	23.234651413639163
92-93	23.40863320664492	26.845041883567927	26.467968238950956	23.278356670836192
94-95	23.17508748083244	26.912070510067494	26.571343552098135	23.341498457001936
96-97	23.138873424314248	26.983352204457024	26.732454559482544	23.145319811746187
98-99	23.373031791394	27.884367418164892	25.756892793491083	22.98570799695003
100	21.63173009529458	27.4030037336065	26.9151581728242	24.050107998274722
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1238.0
1	1214.0
2	1799.5
3	2710.5
4	4017.0
5	7185.5
6	9918.0
7	10926.5
8	10795.0
9	9731.5
10	8500.5
11	7531.5
12	7957.0
13	10985.5
14	18260.5
15	29019.5
16	39029.5
17	45433.5
18	47559.0
19	45060.5
20	40588.5
21	35883.5
22	31999.0
23	31406.0
24	33796.0
25	39411.0
26	48750.0
27	59656.0
28	71382.0
29	84751.0
30	97633.0
31	111546.0
32	130065.0
33	151127.5
34	174575.5
35	198522.5
36	225881.5
37	251233.5
38	270316.0
39	289182.5
40	309892.0
41	327339.0
42	341228.0
43	359228.5
44	378245.0
45	396358.0
46	416020.5
47	435340.0
48	445161.0
49	446767.5
50	449015.0
51	449290.5
52	440953.5
53	427610.0
54	414269.0
55	395715.5
56	371088.0
57	342986.0
58	312854.5
59	281355.5
60	245269.5
61	208131.5
62	173134.0
63	139803.0
64	110311.5
65	86712.0
66	67820.0
67	53614.5
68	42491.5
69	32354.0
70	24397.5
71	18297.0
72	13477.0
73	9695.0
74	7014.0
75	5217.0
76	3935.5
77	2931.5
78	2121.0
79	1547.5
80	1133.0
81	823.5
82	613.5
83	407.5
84	269.5
85	197.0
86	154.5
87	121.0
88	79.5
89	48.0
90	32.0
91	22.0
92	16.5
93	11.0
94	4.5
95	2.0
96	2.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	5.482401436564614E-4
3	0.0028837431556329863
4	0.0026315526895510145
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.315776344775507E-4
12-13	0.0019078756999244854
14-15	0.003207204840390299
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.005900888284415996
26-27	0.018509484870783622
28-29	0.008247226061278604
30-31	0.0
32-33	0.0
34-35	1.9630176449486622E-4
36-37	0.014619707331628057
38-39	0.04098097804336451
40-41	0.04686045804895899
42-43	0.008614535419433699
44-45	3.737557801477398E-4
46-47	0.0
48-49	0.03265496411725281
50-51	0.04826719380968393
52-53	0.05105877152387085
54-55	0.028762377095827556
56-57	9.518775469422194E-4
58-59	0.005886274238573638
60-61	0.026276886142869472
62-63	0.034433750855483086
64-65	0.01897668398986589
66-67	0.049253858542509514
68-69	0.06540426730973473
70-71	0.07275381061288641
72-73	0.04909872402757349
74-75	0.02127464054843171
76-77	0.0045051455778709615
78-79	3.7491166143977325E-4
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.001675508717697768
92-93	0.0021189966177074574
94-95	4.980958901001228E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	3167.0
20-21	9459.0
22-23	11015.0
24-25	19400.0
26-27	26270.0
28-29	37931.0
30-31	40603.0
32-33	44398.0
34-35	51576.0
36-37	59533.0
38-39	62041.0
40-41	78911.0
42-43	89343.0
44-45	100688.0
46-47	108662.0
48-49	94377.0
50-51	108986.0
52-53	100038.0
54-55	111522.0
56-57	117583.0
58-59	117362.0
60-61	120840.0
62-63	110625.0
64-65	121849.0
66-67	127862.0
68-69	116072.0
70-71	120295.0
72-73	111002.0
74-75	105840.0
76-77	94975.0
78-79	122426.0
80-81	133621.0
82-83	138143.0
84-85	148649.0
86-87	159802.0
88-89	163289.0
90-91	171287.0
92-93	186378.0
94-95	221604.0
96-97	376731.0
98-99	127712.0
100-101	4748224.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.97781262746076
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.96519732906849	79.98931701535173
2	5.47175003315306	9.518416982557781
3	1.3650563259242112	3.5618884002649813
4	0.5198343456877245	1.8085621726618226
5	0.24495554027482422	1.065284854204104
6	0.130638175216918	0.6817573635606291
7	0.07997549406434187	0.486926547626185
8	0.05163266552278221	0.35927170458375235
9	0.03841168684792195	0.3006868051226929
>10	0.12768619279593346	1.7736513753220469
>50	0.0033946183446204514	0.19769552692951736
>100	0.0013838391557222803	0.17817628915926048
>500	4.187758624026081E-5	0.021672346006224936
>1k	4.187635727430004E-5	0.05669261664917018
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0964802873129227E-5	2.1929605746258454E-5
2	0.0	2.1929605746258454E-5	0.0	1.0964802873129227E-5	2.1929605746258454E-5
3	1.0964802873129227E-5	2.1929605746258454E-5	0.0	1.0964802873129227E-5	2.1929605746258454E-5
4	1.0964802873129227E-5	1.644720430969384E-4	0.0	1.0964802873129227E-5	2.1929605746258454E-5
5	3.289440861938768E-5	1.7543684597006763E-4	0.0	1.0964802873129227E-5	2.1929605746258454E-5
6	6.578881723877536E-5	1.7543684597006763E-4	0.0	1.0964802873129227E-5	2.1929605746258454E-5
7	6.578881723877536E-5	2.083312545894553E-4	0.0	1.0964802873129227E-5	2.1929605746258454E-5
8	6.578881723877536E-5	2.741200718282307E-4	0.0	1.0964802873129227E-5	3.289440861938768E-5
9	7.675362011190459E-5	4.8245132641768596E-4	0.0	1.0964802873129227E-5	3.289440861938768E-5
10-11	8.771842298503382E-5	4.8245132641768596E-4	0.0	1.0964802873129227E-5	3.289440861938768E-5
12-13	8.771842298503382E-5	4.8245132641768596E-4	0.0	1.0964802873129227E-5	3.289440861938768E-5
14-15	8.771842298503382E-5	7.127121867533997E-4	0.0	1.0964802873129227E-5	4.385921149251691E-5
16-17	1.3157763447755073E-4	7.23676989626529E-4	0.0	1.0964802873129227E-5	4.385921149251691E-5
18-19	1.3157763447755073E-4	8.22360215484692E-4	0.0	1.644720430969384E-5	4.385921149251691E-5
20-21	1.3706003591411534E-4	9.539378499622427E-4	0.0	2.741200718282307E-5	4.385921149251691E-5
22-23	1.4254243735067996E-4	0.0010471386743838413	0.0	3.289440861938768E-5	5.482401436564614E-5
24-25	1.4254243735067996E-4	0.0012445051261001671	0.0	5.482401436564614E-5	6.578881723877536E-5
26-27	1.5350724022380918E-4	0.0013267411476486364	0.0	1.4802483878724457E-4	7.127121867533998E-5
28-29	1.5350724022380918E-4	0.0013377059505217657	0.0	4.660041221079921E-4	7.675362011190459E-5
30-31	1.5350724022380918E-4	0.0015295900008015272	0.0	0.0013870475634508472	7.675362011190459E-5
32-33	1.5350724022380918E-4	0.0015460372051112209	0.0	0.0032675112561925098	7.675362011190459E-5
34-35	1.5350724022380918E-4	0.0017598508611372408	0.0	0.005778451114139102	8.771842298503382E-5
36-37	1.5350724022380918E-4	0.001973664517163261	0.0	0.009237846420611373	8.771842298503382E-5
38-39	1.5350724022380918E-4	0.002028488531528907	0.0	0.015657738502828533	8.771842298503382E-5
40-41	1.5350724022380918E-4	0.0022806789976108793	0.0	0.022209208219523248	8.771842298503382E-5
42-43	1.5350724022380918E-4	0.0022916438004840084	0.0	0.029374706897113197	8.771842298503382E-5
44-45	1.5350724022380918E-4	0.0025054574565100286	0.0	0.038283609231530694	1.0416562729472765E-4
46-47	1.5350724022380918E-4	0.0026863767039166605	0.0	0.045991865651340544	1.2609523304098612E-4
48-49	1.589896416603738E-4	0.0026973415067897896	0.0	0.053223153146169266	1.3157763447755073E-4
50-51	1.8640164884319686E-4	0.003103039213095571	0.0	0.05990071809590496	1.3157763447755073E-4
52-53	1.8640164884319686E-4	0.0031359336217149586	0.0	0.06757608010709543	1.3157763447755073E-4
54-55	1.9188405027976147E-4	0.0031414160231515234	0.0	0.07430298666976021	1.3157763447755073E-4
56-57	2.028488531528907E-4	0.0031688280303343464	0.0	0.08038296986291035	1.4802483878724457E-4
58-59	2.1381365602601992E-4	0.0031907576360806046	0.0	0.08605177294831817	1.644720430969384E-4
60-61	2.3574326177227837E-4	0.003245581650446251	0.0	0.09154513918775592	1.644720430969384E-4
62-63	2.5219046608197223E-4	0.003289440861938768	0.0	0.09791568965704399	2.083312545894553E-4
64-65	2.5219046608197223E-4	0.0036622441596251614	0.0	0.10297046378155655	2.083312545894553E-4
66-67	2.5767286751853685E-4	0.003711585772554243	0.0	0.10793203708164753	2.083312545894553E-4
68-69	2.6315526895510146E-4	0.0037938217941027126	0.0	0.11404491468341708	2.083312545894553E-4
70-71	2.796024732647953E-4	0.003843163407031794	0.0	0.11882556873610142	2.083312545894553E-4
72-73	3.12496881884183E-4	0.0038815402170877464	0.0	0.12485072791488594	2.4122566320884298E-4
74-75	3.289440861938768E-4	0.004024082654438426	0.0	0.1295491459460218	2.4122566320884298E-4
76-77	3.4539129050357065E-4	0.004133730683169718	0.0	0.1334526157688558	2.4122566320884298E-4
78-79	3.5087369194013526E-4	0.004194037098971929	0.0	0.13582649559088827	2.4122566320884298E-4
80-81	3.563560933766999E-4	0.004215966704718188	0.0	0.13715323673853694	2.4122566320884298E-4
82-83	3.618384948132645E-4	0.00425434351477414	0.0	0.13976285982234168	2.4122566320884298E-4
84-85	3.618384948132645E-4	0.004265308317647269	0.0	0.14178586595243403	2.4122566320884298E-4
86-87	3.728032976863937E-4	0.004292720324830092	0.0	0.14277269821101565	2.4122566320884298E-4
88	3.8376810055952294E-4	0.004462674769363595	0.0	0.14289331104262007	2.5219046608197223E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10690	0.0	49.879124	1
GTATCAA	20080	0.0	37.752678	1
CTTATAC	7900	0.0	32.508667	1
TATACAC	9280	0.0	29.955336	3
TCAACGC	25460	0.0	29.162476	4
TATCAAC	26025	0.0	28.683613	2
ATCAACG	25890	0.0	28.632471	3
CAACGCA	26210	0.0	28.190228	5
AACGCAG	26910	0.0	27.56187	6
TTATACA	10560	0.0	25.492432	2
GTACATG	36935	0.0	25.066322	1
TACATGG	36590	0.0	25.00794	2
ACATGGG	37355	0.0	23.993135	3
ACGCAGA	31685	0.0	23.294788	7
TACACAT	12130	0.0	22.817408	5
CGCAGAG	32690	0.0	22.640339	8
CATGGGG	28810	0.0	21.959137	4
GTATAAT	6060	0.0	21.618048	1
ACACATC	12360	0.0	21.391933	6
ACATCTC	12840	0.0	20.87276	8
>>END_MODULE