FastQCFastQC Report
Fri 27 May 2016
SRR2139346_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139346_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10424560
Sequences flagged as poor quality0
Sequence length19-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA139700.051.4986531
GTATCAA262050.038.9183581
TCAACGC331700.030.1597864
ATCAACG335250.029.828953
CAACGCA337150.029.6473165
TATCAAC340850.029.5451182
AACGCAG348650.028.7413566
CTTATAC74850.026.8310681
TATACAC86700.025.9281333
TACATGG456800.025.0489982
GTGGTAT57350.024.93081
GTACATG471150.024.8035431
ACGCAGA411550.024.2783327
ACATGGG468100.023.9939753
CGCAGAG423650.023.565638
TGGTATC57750.022.754572
CATGGGG347000.022.030344
GCAGAGT481550.020.5270149
GAGTACT233450.019.41831212-13
GTATAAT78900.019.2736661
Produced by FastQC (version 0.11.5)