##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139346_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10424560
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60496289531645	33.0	33.0	33.0	33.0	33.0
2	32.609212091445585	33.0	33.0	33.0	33.0	33.0
3	32.658888528628545	33.0	33.0	33.0	33.0	33.0
4	36.572209474548565	37.0	37.0	37.0	37.0	37.0
5	36.5953862800924	37.0	37.0	37.0	37.0	37.0
6	36.20238839816741	37.0	37.0	37.0	33.0	37.0
7	36.06333600650771	37.0	37.0	37.0	33.0	37.0
8	35.984999942443615	37.0	37.0	37.0	33.0	37.0
9	35.97702128435157	37.0	37.0	37.0	33.0	37.0
10-11	35.91688891425633	37.0	37.0	37.0	33.0	37.0
12-13	35.824931268082295	37.0	37.0	37.0	33.0	37.0
14-15	37.52257083272579	40.0	37.0	40.0	33.0	40.0
16-17	37.46363482007874	40.0	37.0	40.0	33.0	40.0
18-19	37.49723177764817	40.0	37.0	40.0	33.0	40.0
20-21	37.40707974056788	40.0	37.0	40.0	33.0	40.0
22-23	37.287483022798554	40.0	37.0	40.0	33.0	40.0
24-25	37.27445303622774	40.0	37.0	40.0	33.0	40.0
26-27	37.12476389514411	40.0	37.0	40.0	33.0	40.0
28-29	36.965144368637425	40.0	37.0	40.0	33.0	40.0
30-31	36.78137183837342	40.0	37.0	40.0	33.0	40.0
32-33	36.563870838327574	40.0	37.0	40.0	33.0	40.0
34-35	36.46418898959976	40.0	37.0	40.0	33.0	40.0
36-37	36.282639443938756	37.0	37.0	40.0	33.0	40.0
38-39	36.30321554596163	37.0	37.0	40.0	33.0	40.0
40-41	36.33374266194019	37.0	37.0	40.0	33.0	40.0
42-43	36.24244389537849	37.0	37.0	40.0	33.0	40.0
44-45	36.11084772041478	37.0	37.0	40.0	27.0	40.0
46-47	35.934842853673956	37.0	37.0	40.0	27.0	40.0
48-49	35.71345007786494	37.0	37.0	40.0	27.0	40.0
50-51	35.6807024982062	37.0	37.0	40.0	27.0	40.0
52-53	35.55561288990974	37.0	35.0	40.0	27.0	40.0
54-55	35.347458016754345	37.0	33.0	40.0	27.0	40.0
56-57	35.11009939327687	37.0	33.0	40.0	27.0	40.0
58-59	34.975355830633504	37.0	33.0	40.0	27.0	40.0
60-61	34.83362601915698	37.0	33.0	40.0	27.0	40.0
62-63	34.65442511958889	37.0	33.0	40.0	27.0	40.0
64-65	34.452367797254354	37.0	33.0	38.5	27.0	40.0
66-67	34.17359467281034	37.0	33.0	37.0	27.0	40.0
68-69	33.94136057565123	37.0	33.0	37.0	27.0	40.0
70-71	33.707458403835034	37.0	33.0	37.0	27.0	40.0
72-73	33.417088226178905	37.0	33.0	37.0	27.0	40.0
74-75	33.07482847983741	37.0	33.0	37.0	24.5	37.0
76-77	30.8812847747636	33.0	30.0	35.0	22.0	37.0
78-79	33.041359297186894	35.0	33.0	37.0	27.0	37.0
80-81	33.43970276815951	37.0	33.0	37.0	27.0	37.0
82-83	33.40372356362463	37.0	33.0	37.0	27.0	37.0
84-85	33.31565062188317	37.0	33.0	37.0	27.0	37.0
86-87	33.17998678648129	35.0	33.0	37.0	27.0	37.0
88-89	33.0129196830016	33.0	33.0	37.0	27.0	37.0
90-91	32.922047413222174	33.0	33.0	37.0	27.0	37.0
92-93	32.965063872331015	33.0	33.0	37.0	27.0	37.0
94-95	33.01287251884115	33.0	33.0	37.0	27.0	37.0
96-97	33.11203061895971	33.0	33.0	37.0	27.0	37.0
98-99	33.24906797111979	33.0	33.0	37.0	27.0	37.0
100	33.21703521320314	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	7.0
15	18.0
16	52.0
17	127.0
18	476.0
19	1247.0
20	2992.0
21	7619.0
22	18816.0
23	41807.0
24	78780.0
25	130008.0
26	189819.0
27	250307.0
28	304384.0
29	346418.0
30	379344.0
31	409490.0
32	445557.0
33	510029.0
34	621687.0
35	845531.0
36	1390695.0
37	2646896.0
38	1762220.0
39	40231.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.52203834022731	19.46061032791792	13.239196666334118	23.778154665520653
2	15.470072810928526	21.028047872451662	40.306434800826594	23.195444515793223
3	18.69350353959507	26.654870414702692	29.73989887967583	24.91172716602641
4	12.928129508459824	17.330213783316495	37.76119748050513	31.980459227718548
5	13.497288564795056	38.53371938345941	34.56240281957413	13.406589232171392
6	30.909553976378863	36.82224477579869	18.443243647693524	13.824957600128926
7	27.697946004435682	32.352588502536314	22.277813164296624	17.67165232873138
8	26.359875141013145	35.116666794569745	20.703952972595484	17.819505091821622
9	27.381769590275272	15.7464487709793	19.95323543631578	36.918546202429646
10-11	25.59985311599002	26.098037594325397	27.86099584903025	20.44111344065433
12-13	26.435624563793485	23.57970726636658	27.874298466246206	22.11036970359373
14-15	23.510136213705636	24.66346124329062	25.625529536632424	26.20087300637132
16-17	23.010457995349444	27.637420668114533	26.19779156146638	23.154329775069645
18-19	23.103795268097645	26.76286097446799	27.89277437129241	22.240569386141956
20-21	24.279391186599515	26.05128901091745	27.4407001134316	22.228619689051442
22-23	23.792608925505167	25.964061078098492	27.486052082057043	22.75727791433929
24-25	23.45775246306307	26.173198045804053	27.662385190507173	22.706664300625704
26-27	23.29517741187954	26.413162197891328	27.604403538937376	22.68725685129175
28-29	23.190971742556144	26.33811434685292	27.688478751338252	22.78243515925268
30-31	23.6206252241702	26.266695241525017	27.46985933345701	22.642820200847773
32-33	23.277482441330342	26.465997129275245	27.543083250239555	22.713437179154862
34-35	23.328310888335718	26.46162480411048	27.487768843377587	22.722295464176216
36-37	23.44761223215653	26.366512154802436	27.478619641658124	22.70725597138291
38-39	23.207946733225803	26.57759180552443	27.54827602261285	22.666185438636916
40-41	23.44350751545072	26.439641780482326	27.462664971569907	22.65418573249705
42-43	23.44182413284934	26.37372362236068	27.26852003209428	22.915932212695708
44-45	23.35329368441513	26.68963811562717	27.083533606237985	22.873534593719715
46-47	23.63374770692986	26.4711208560924	26.617382027252756	23.277749409724983
48-49	23.391622122394427	26.574789081589874	26.821077784412473	23.212511011603222
50-51	23.24600134980706	26.759950931594368	26.738437537558845	23.255610181039728
52-53	23.51502419438126	26.752549793069395	26.53775801252583	23.194668000023512
54-55	23.522471269435634	26.61153156758572	26.50497898942349	23.361018173555163
56-57	23.575000751800605	26.919651939329853	26.331016146536584	23.174331162332958
58-59	23.352386665783687	26.97914218451953	26.637831296202275	23.030639853494502
60-61	23.516685233628763	27.058930759892863	26.371091365995632	23.053292640482738
62-63	23.413983195163965	27.237175556052623	26.26772880200462	23.08111244677879
64-65	23.33270527663846	27.164317309458824	26.40589969009654	23.097077723806176
66-67	23.580637533243063	27.08655955977561	26.192788888894558	23.14001401808677
68-69	23.37162445690674	26.980381097791145	26.41925625539347	23.22873818990864
70-71	23.413442086828386	26.57453517755956	26.56552611775841	23.446496617853644
72-73	23.506333388497815	26.707778909688685	26.463196761833046	23.322690939980454
74-75	23.122712261523162	26.976672038587456	26.505940286896955	23.39467541299242
76-77	23.309405090157153	26.892972343982724	26.44241327996177	23.355209285898354
78-79	23.301861791400164	26.92856923474174	26.34399686599722	23.425572107860877
80-81	23.346450220284545	26.858415607017072	26.36971918913917	23.425414983559214
82-83	23.336034977497622	26.76927787952359	26.59163081319781	23.303056329780976
84-85	23.502426115489058	26.72615738252715	26.423515055810604	23.347901446173186
86-87	23.0595289996425	26.871806053832124	26.57954949942438	23.489115447100993
88-89	23.279901620386052	26.743272999132827	26.584622851820498	23.392202528660626
90-91	23.30026724860018	26.703405458169833	26.686685511327358	23.30964178190263
92-93	23.35744852028325	26.72944105754493	26.58902228418052	23.324088137991303
94-95	23.197004695476515	26.801153437252722	26.620950283576168	23.38089158369459
96-97	23.146234833735697	26.870515493256597	26.82838980901962	23.154859863988086
98-99	24.300910631689828	28.85650749111555	26.893497193414294	19.949084683780324
100	23.438412506632865	29.67519659454474	20.58536980985723	26.30102108896516
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1586.0
1	1521.5
2	2262.5
3	3477.5
4	5208.0
5	9275.5
6	12660.0
7	13911.0
8	13743.0
9	12242.5
10	10396.0
11	9021.5
12	9747.5
13	14169.0
14	24444.0
15	38986.5
16	51763.0
17	59612.0
18	61680.0
19	57211.0
20	49876.0
21	42183.0
22	36491.5
23	35462.5
24	38176.0
25	45151.5
26	57199.5
27	71503.0
28	83931.0
29	99182.5
30	114902.5
31	130356.5
32	152701.5
33	177039.0
34	204016.5
35	231614.5
36	262395.5
37	291657.0
38	308561.5
39	323069.0
40	345718.0
41	364673.5
42	376307.0
43	393608.5
44	410993.0
45	426589.0
46	440728.0
47	453844.5
48	471750.0
49	480397.5
50	476537.0
51	473560.0
52	468922.0
53	457956.5
54	442615.5
55	421744.5
56	396832.5
57	368096.5
58	336199.5
59	303801.0
60	265292.5
61	222198.0
62	180613.0
63	143880.0
64	112535.5
65	87177.0
66	67629.5
67	53472.5
68	42609.5
69	32235.0
70	23555.5
71	17264.0
72	12655.0
73	9073.0
74	6477.5
75	4794.5
76	3648.5
77	2705.5
78	1951.5
79	1411.5
80	1001.5
81	695.5
82	534.0
83	382.0
84	272.0
85	206.0
86	151.5
87	112.0
88	73.0
89	47.0
90	36.0
91	28.5
92	22.5
93	13.0
94	5.5
95	3.5
96	3.0
97	3.0
98	2.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	2.494110063158541E-4
3	0.0033094921991911413
4	0.0039042415219443316
5	6.714911708503765E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	7.674184809718588E-5
12-13	0.0016163751755469775
14-15	0.0033766413162761785
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.006664866287202071
26-27	0.020966855313763303
28-29	0.009237726663108467
30-31	0.0
32-33	0.0
34-35	2.0518344181349332E-4
36-37	0.011793145576444097
38-39	0.03313180779764985
40-41	0.0379581602733503
42-43	0.0065631731619621445
44-45	2.66750214847166E-4
46-47	0.0
48-49	0.03433279103285062
50-51	0.05862103780467769
52-53	0.05517622194352053
54-55	0.02918520099989378
56-57	0.0012627950298527955
58-59	0.005913238561738079
60-61	0.029060743305583708
62-63	0.03385902714642936
64-65	0.014727699491388156
66-67	0.039205332061171876
68-69	0.05592216341768996
70-71	0.07369207457651886
72-73	0.05252568239008314
74-75	0.02297392153784637
76-77	0.004888264424118312
78-79	3.4598222076275725E-4
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0020020247504367933
92-93	0.0024551528873638278
94-95	4.997289327489075E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	3039.0
20-21	9071.0
22-23	10455.0
24-25	18121.0
26-27	24504.0
28-29	35153.0
30-31	37071.0
32-33	40513.0
34-35	46509.0
36-37	47040.0
38-39	49920.0
40-41	67289.0
42-43	77766.0
44-45	96001.0
46-47	112305.0
48-49	87693.0
50-51	106699.0
52-53	93441.0
54-55	92590.0
56-57	99358.0
58-59	112017.0
60-61	100585.0
62-63	92389.0
64-65	109332.0
66-67	116245.0
68-69	102083.0
70-71	107509.0
72-73	95907.0
74-75	89371.0
76-77	79947.0
78-79	109493.0
80-81	126774.0
82-83	127556.0
84-85	140524.0
86-87	153424.0
88-89	168948.0
90-91	181469.0
92-93	195956.0
94-95	230643.0
96-97	427158.0
98-99	632186.0
100-101	5770506.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.01148356281028
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.2083872060629	75.78540438989779
2	6.372944137907623	10.708009833770687
3	1.7671749396682024	4.453889651896363
4	0.7130899464872259	2.396309772724674
5	0.34514895569729737	1.4498237909142317
6	0.18593406874130713	0.9372358175896057
7	0.11287147049876306	0.6637749781961942
8	0.07143832484060605	0.4801311722481022
9	0.04959160014779518	0.37496375106029595
>10	0.16729468162836578	2.1952675028075475
>50	0.004448126341880034	0.24889809568626672
>100	0.0015922787110291164	0.19805207830471605
>500	0.0	0.0
>1k	8.426326706182558E-5	0.10823916490359342
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.592731012148235E-6
2	0.0	2.8778193036444703E-5	0.0	0.0	9.592731012148235E-6
3	0.0	3.837092404859294E-5	0.0	0.0	9.592731012148235E-6
4	0.0	7.674184809718588E-5	0.0	0.0	1.918546202429647E-5
5	0.0	8.633457910933411E-5	0.0	0.0	1.918546202429647E-5
6	2.8778193036444703E-5	8.633457910933411E-5	0.0	0.0	1.918546202429647E-5
7	2.8778193036444703E-5	8.633457910933411E-5	0.0	0.0	1.918546202429647E-5
8	2.8778193036444703E-5	1.0552004113363058E-4	0.0	0.0	3.837092404859294E-5
9	2.8778193036444703E-5	2.0144735125511294E-4	0.0	0.0	7.674184809718588E-5
10-11	2.8778193036444703E-5	2.0144735125511294E-4	0.0	4.7963655060741175E-6	7.674184809718588E-5
12-13	2.8778193036444703E-5	2.0144735125511294E-4	0.0	9.592731012148235E-6	8.153821360326E-5
14-15	2.8778193036444703E-5	3.1656012340089174E-4	0.0	9.592731012148235E-6	1.4868733068829765E-4
16-17	4.796365506074117E-5	3.3574558542518823E-4	0.0	9.592731012148235E-6	1.918546202429647E-4
18-19	4.796365506074117E-5	3.69320143967707E-4	0.0	1.4389096518222353E-5	1.918546202429647E-4
20-21	4.796365506074117E-5	4.028947025102259E-4	0.0	3.357455854251882E-5	1.966509857490388E-4
22-23	5.276002056681529E-5	5.036183781377823E-4	0.0	7.194548259111176E-5	2.5900373732800236E-4
24-25	5.7556386072889406E-5	5.803602262349682E-4	0.0	1.2470550315792705E-4	3.117637578948176E-4
26-27	5.7556386072889406E-5	6.091384192714129E-4	0.0	1.678727927125941E-4	3.3574558542518823E-4
28-29	5.7556386072889406E-5	6.13934784777487E-4	0.0	3.7411650947378116E-4	3.3574558542518823E-4
30-31	5.7556386072889406E-5	7.002693638868212E-4	0.0	0.001151127721457788	3.3574558542518823E-4
32-33	5.7556386072889406E-5	7.098620948989693E-4	0.0	0.0031943794270453624	3.4533831643733645E-4
34-35	5.7556386072889406E-5	9.304949081783787E-4	0.0	0.005736453145264645	3.5493104744948467E-4
36-37	5.7556386072889406E-5	0.0011463313559517138	0.0	0.009343320005832382	3.645237784616329E-4
38-39	5.7556386072889406E-5	0.0011942950110124552	0.0	0.015895155287129623	3.8850560599200347E-4
40-41	5.7556386072889406E-5	0.0013717605347371975	0.0	0.022873867098467466	3.933019714980776E-4
42-43	5.7556386072889406E-5	0.0014053350932797164	0.0	0.02957438971045301	3.933019714980776E-4
44-45	6.235275157896352E-5	0.0015636151549801624	0.0	0.03745481823693278	3.9809833700415174E-4
46-47	6.714911708503765E-5	0.0016883206581380893	0.0	0.04546474863207656	4.2687653004059645E-4
48-49	6.714911708503765E-5	0.0016883206581380893	0.0	0.05235232949879899	4.988220126317082E-4
50-51	8.633457910933411E-5	0.001928138933441795	0.0	0.0585588264636589	4.988220126317082E-4
52-53	9.592731012148235E-5	0.0019473243954660916	0.0	0.06464541429086695	5.563783987045976E-4
54-55	9.592731012148235E-5	0.00195691712647824	0.0	0.07054014749783205	6.235275157896352E-4
56-57	9.592731012148235E-5	0.001976102588502536	0.0	0.07550438579661875	6.427129778139317E-4
58-59	9.592731012148235E-5	0.0019904916850207585	0.0	0.07953333282172101	6.57102074332154E-4
60-61	1.0552004113363058E-4	0.0020336589745754254	0.0	0.08411386188002179	6.618984398382282E-4
62-63	1.342982341700753E-4	0.0021295862846969077	0.0	0.0890589147167842	7.098620948989693E-4
64-65	1.342982341700753E-4	0.0023118481739277247	0.0	0.09323654907257477	7.098620948989693E-4
66-67	1.390945996761494E-4	0.002326237270445947	0.0	0.0980281182131428	7.242511914171918E-4
68-69	1.438909651822235E-4	0.002374200925506688	0.0	0.10340004757994582	7.290475569232658E-4
70-71	1.4868733068829765E-4	0.0023981827530370585	0.0	0.10717958359873223	7.626221154657846E-4
72-73	1.5348369619437176E-4	0.0024173682150613555	0.0	0.11208146914593997	9.113094461540823E-4
74-75	1.5348369619437176E-4	0.0024605355046160224	0.0	0.11577467058561705	9.113094461540823E-4
76-77	1.5348369619437176E-4	0.002532480987207134	0.0	0.11900742093671099	9.113094461540823E-4
78-79	1.5348369619437176E-4	0.002580444642267875	0.0	0.12076289071193412	9.113094461540823E-4
80-81	1.5348369619437176E-4	0.0025852410077739493	0.0	0.12165021833055784	9.400876391905269E-4
82-83	1.5348369619437176E-4	0.0025900373732800235	0.0	0.12341048447128704	9.88051294251268E-4
84-85	1.5348369619437176E-4	0.0026092228353043197	0.0	0.1250700269363887	9.88051294251268E-4
86-87	1.5828006170044587E-4	0.0026475937593529127	0.0	0.12597654001703668	9.88051294251268E-4
88	1.6307642720651998E-4	0.0027627065314986916	0.0	0.12607726369266425	9.88051294251268E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13970	0.0	51.498653	1
GTATCAA	26205	0.0	38.918358	1
TCAACGC	33170	0.0	30.159786	4
ATCAACG	33525	0.0	29.82895	3
CAACGCA	33715	0.0	29.647316	5
TATCAAC	34085	0.0	29.545118	2
AACGCAG	34865	0.0	28.741356	6
CTTATAC	7485	0.0	26.831068	1
TATACAC	8670	0.0	25.928133	3
TACATGG	45680	0.0	25.048998	2
GTGGTAT	5735	0.0	24.9308	1
GTACATG	47115	0.0	24.803543	1
ACGCAGA	41155	0.0	24.278332	7
ACATGGG	46810	0.0	23.993975	3
CGCAGAG	42365	0.0	23.56563	8
TGGTATC	5775	0.0	22.75457	2
CATGGGG	34700	0.0	22.03034	4
GCAGAGT	48155	0.0	20.527014	9
GAGTACT	23345	0.0	19.418312	12-13
GTATAAT	7890	0.0	19.273666	1
>>END_MODULE