FastQCFastQC Report
Fri 27 May 2016
SRR2139345_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139345_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14639158
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA176550.047.891421
GTATCAA324850.037.506651
TCAACGC400050.029.7294254
ATCAACG403800.029.4949463
TATCAAC413700.029.0264072
CAACGCA409450.029.0056825
AACGCAG423450.028.1533836
CTTATAC110350.025.6764981
TACATGG505800.024.0724752
ACGCAGA493350.024.0569257
GTACATG515850.023.9997831
CGCAGAG506000.023.4476628
ACATGGG513850.023.2019563
TATACAC142800.023.1629413
CATGGGG370250.020.8921534
GCAGAGT584750.020.2145089
GTGGTAT80500.019.9097161
GAGTACT320650.018.71872912-13
TGGTATC81500.018.2607522
AGAGTAC523300.017.94324510-11
Produced by FastQC (version 0.11.5)