FastQCFastQC Report
Fri 27 May 2016
SRR2139344_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139344_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8569160
Sequences flagged as poor quality0
Sequence length19-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111000.052.886631
AATCTCG81250.043.431466
CTCGTAT85450.041.5476579
GTATCAA213600.040.5422061
TCTCGTA86900.040.3659178
ATCTCGT90200.039.0751657
TCAACGC276000.030.9457534
TATCAAC278650.030.8199042
ATCAACG277550.030.6982363
CAACGCA278350.030.6376935
GAAATCT120000.029.7590664
AAATCTC120850.029.723395
AACGCAG287650.029.7216426
GTACATG389950.026.3151841
TACATGG383200.026.2603512
ACGCAGA334800.025.3970837
ACATGGG390750.025.2985323
CGCAGAG344950.024.6261448
CATGGGG302300.022.9271244
GCAGAAA172800.021.8900091
Produced by FastQC (version 0.11.5)