##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139344_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8569160
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.63557793295959	33.0	33.0	33.0	33.0	33.0
2	32.631476013985036	33.0	33.0	33.0	33.0	33.0
3	32.7078558458472	33.0	33.0	33.0	33.0	33.0
4	36.63883857927731	37.0	37.0	37.0	37.0	37.0
5	36.6641319569246	37.0	37.0	37.0	37.0	37.0
6	36.30717304846683	37.0	37.0	37.0	37.0	37.0
7	36.1923402060412	37.0	37.0	37.0	33.0	37.0
8	36.089216329255144	37.0	37.0	37.0	33.0	37.0
9	36.07533725592707	37.0	37.0	37.0	33.0	37.0
10-11	36.04116179415485	37.0	37.0	37.0	33.0	37.0
12-13	35.94909040092611	37.0	37.0	37.0	33.0	37.0
14-15	37.73320943943163	40.0	37.0	40.0	33.0	40.0
16-17	37.68156767991262	40.0	37.0	40.0	33.0	40.0
18-19	37.71922936437177	40.0	37.0	40.0	33.0	40.0
20-21	37.62408759427318	40.0	37.0	40.0	33.0	40.0
22-23	37.50106952619004	40.0	37.0	40.0	33.0	40.0
24-25	37.49353284360426	40.0	37.0	40.0	33.0	40.0
26-27	37.35185327094639	40.0	37.0	40.0	33.0	40.0
28-29	37.19551497741044	40.0	37.0	40.0	33.0	40.0
30-31	36.99589912807092	40.0	37.0	40.0	33.0	40.0
32-33	36.801296072594596	40.0	37.0	40.0	33.0	40.0
34-35	36.717025182348245	40.0	37.0	40.0	33.0	40.0
36-37	36.50857888815697	40.0	37.0	40.0	33.0	40.0
38-39	36.54901720809406	40.0	37.0	40.0	33.0	40.0
40-41	36.587149219349385	40.0	37.0	40.0	33.0	40.0
42-43	36.491631216793294	40.0	37.0	40.0	33.0	40.0
44-45	36.34517896159164	38.5	37.0	40.0	33.0	40.0
46-47	36.14637674072327	37.0	37.0	40.0	30.0	40.0
48-49	35.92675403579578	37.0	37.0	40.0	27.0	40.0
50-51	35.919411453528106	37.0	37.0	40.0	27.0	40.0
52-53	35.776257632248836	37.0	37.0	40.0	27.0	40.0
54-55	35.57209652713248	37.0	35.0	40.0	27.0	40.0
56-57	35.31770420129868	37.0	33.0	40.0	27.0	40.0
58-59	35.16298743958643	37.0	33.0	40.0	27.0	40.0
60-61	35.01725514321325	37.0	33.0	40.0	27.0	40.0
62-63	34.82729487842694	37.0	33.0	40.0	27.0	40.0
64-65	34.65593173082442	37.0	33.0	38.5	27.0	40.0
66-67	34.39642399686663	37.0	33.0	37.0	27.0	40.0
68-69	34.19721351624101	37.0	33.0	37.0	27.0	40.0
70-71	33.97192927288275	37.0	33.0	37.0	27.0	40.0
72-73	33.687513679557426	37.0	33.0	37.0	27.0	40.0
74-75	33.342933500398146	37.0	33.0	37.0	27.0	37.0
76-77	31.211425115196143	33.0	30.0	35.0	24.5	37.0
78-79	33.34215573073823	35.0	33.0	37.0	27.0	37.0
80-81	33.72415056433457	37.0	33.0	37.0	27.0	37.0
82-83	33.688925295255956	37.0	33.0	37.0	27.0	37.0
84-85	33.59788391233268	37.0	33.0	37.0	27.0	37.0
86-87	33.47243924150895	37.0	33.0	37.0	27.0	37.0
88-89	33.30902358301455	37.0	33.0	37.0	27.0	37.0
90-91	33.22665633736615	33.0	33.0	37.0	27.0	37.0
92-93	33.26482454533834	33.0	33.0	37.0	27.0	37.0
94-95	33.31147955363335	33.0	33.0	37.0	27.0	37.0
96-97	33.411313608874394	33.0	33.0	37.0	27.0	37.0
98-99	33.53853929689408	33.0	33.0	37.0	27.0	37.0
100	33.500977136164806	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	4.0
15	15.0
16	60.0
17	194.0
18	471.0
19	1250.0
20	2891.0
21	6342.0
22	13816.0
23	28986.0
24	54307.0
25	89900.0
26	134173.0
27	181324.0
28	228618.0
29	269868.0
30	302832.0
31	334437.0
32	371483.0
33	422746.0
34	508383.0
35	665574.0
36	1065885.0
37	2258184.0
38	1609523.0
39	17890.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.16845991905858	19.192499614898075	13.014636207049465	23.62440425899388
2	15.486713735715703	20.785325155590783	40.202924153293345	23.525036955400168
3	18.60903128710394	26.964042015518096	29.458535432008603	24.968391265369362
4	13.282587600574871	17.13261908082342	37.61841270693377	31.966380611667937
5	13.514038021947552	38.6897262694249	34.34152675012568	13.454708958501865
6	30.73940736314878	37.030875838471914	18.474914694089037	13.75480210429027
7	27.74491315368134	32.56393858907991	22.257327439328943	17.433820817909805
8	26.250320918269704	34.815174416162144	21.06687236555275	17.867632300015405
9	27.32034411774316	15.594165589159264	19.93509282123335	37.150397471864224
10-11	25.572160082640643	25.8044468593089	27.989844732539954	20.633548325510503
12-13	26.681811941041726	23.435275687347527	27.94137992261533	21.941532448995417
14-15	23.46998781405191	24.681901474707445	25.736005823860886	26.11210488737976
16-17	23.14148644674624	27.325035359358445	26.10202166840157	23.431456525493747
18-19	23.164930985067382	26.550968823081845	27.859510150353128	22.42459004149765
20-21	24.17250471183588	25.752453263287496	27.69675070863618	22.378291316240446
22-23	23.751590555634102	25.689965215176326	27.907448065218333	22.650996163971243
24-25	23.321032178734928	25.96908877254604	27.85351188235622	22.856367166362812
26-27	23.458628722768132	26.170217901815462	27.502137607681583	22.869015767734822
28-29	23.144578226201965	26.132149115028884	28.020614599807338	22.70265805896181
30-31	23.852030496829478	26.268475313500428	27.29502418630957	22.584470003360526
32-33	23.22139275249645	26.864568968941704	27.282277201783405	22.631761076778442
34-35	23.349684040160536	26.734328419853064	27.40490302828794	22.511084511698456
36-37	23.4762586419872	26.530215560834453	27.301871351774782	22.691654445403564
38-39	23.26083050117738	26.547254122063475	27.53818599968456	22.653729377074587
40-41	23.531574413400634	26.280400369220402	27.483784708393483	22.704240508985478
42-43	23.4493902192757	26.223525835626283	27.280381917293568	23.046702027804457
44-45	23.415661861403354	26.542308241346042	27.03890566673349	23.003124230517106
46-47	23.656348494287684	26.3590797012006	26.62941079712179	23.35516100738993
48-49	23.483319624071143	26.463312472709948	26.756758235254917	23.296609667963992
50-51	23.393747985013196	26.731924590194723	26.584435381133776	23.28989204365831
52-53	23.58886827158768	26.64539526166117	26.38135340086787	23.38438306588328
54-55	23.660945698882745	26.504235327151054	26.38078808430548	23.454030889660725
56-57	23.58266610891791	26.824593551664545	26.28471098043595	23.3080293589816
58-59	23.429746835239172	26.85827611408825	26.464309653579438	23.24766739709313
60-61	23.569956852629463	26.90265676450267	26.349735051172335	23.177651331695532
62-63	23.550336156305583	26.97486022223889	26.24196187083322	23.232841750622313
64-65	23.447800427929362	26.970088113377226	26.271705815535622	23.310405643157793
66-67	23.636037681690627	26.896775144160106	26.12949780030487	23.337689373844402
68-69	23.425595306852806	26.838619875600866	26.361646394633333	23.374138422912992
70-71	23.503246337495792	26.49105571857442	26.4841856648945	23.521512279035292
72-73	23.55595384845887	26.596943849696153	26.337462870410295	23.509639431434678
74-75	23.27344735222772	26.87567550133803	26.36965638513688	23.481220761297372
76-77	23.470096558869503	26.725701578479153	26.336915454768818	23.467286407882522
78-79	23.3114923529774	26.76671452208389	26.318415113579086	23.60337801135962
80-81	23.39985071777715	26.702287334450723	26.379485184668074	23.518376763104058
82-83	23.375526046484996	26.603676974438635	26.622974686228346	23.397822292848026
84-85	23.543392594617828	26.61626358489451	26.355933504251126	23.484410316236534
86-87	23.19030871721974	26.765057467878016	26.545320630618356	23.49931318428389
88-89	23.38249849574472	26.623054464591373	26.47514823100105	23.51929880866286
90-91	23.37694867717711	26.55865387612139	26.629522540795158	23.43487490590634
92-93	23.401415306378315	26.591228950130574	26.56210476638811	23.445250977102997
94-95	23.19343399601157	26.627030114381572	26.66777074890302	23.511765140703837
96-97	23.234874129343	26.667362172412197	26.824578236789275	23.273185461455522
98-99	24.333069431845328	28.7302699678109	26.89182285550384	20.04483774483993
100	23.577592337270296	29.448642359189286	20.64579365170719	26.327971651833227
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1201.0
1	1219.0
2	1918.5
3	2911.0
4	4383.5
5	7854.5
6	10619.0
7	11574.5
8	11348.5
9	10014.0
10	8430.5
11	7237.5
12	8075.0
13	12047.0
14	20835.0
15	33371.0
16	44468.0
17	50949.0
18	51991.5
19	47269.0
20	40485.0
21	33700.5
22	28494.0
23	27042.5
24	28549.5
25	33539.0
26	42264.0
27	52979.0
28	62088.5
29	73654.5
30	86809.5
31	100111.5
32	119093.0
33	139192.0
34	159997.5
35	181566.5
36	207662.0
37	236123.5
38	255630.5
39	271168.5
40	291598.5
41	305373.5
42	307655.5
43	312411.5
44	322754.5
45	336463.0
46	347582.0
47	357606.5
48	367812.0
49	372933.5
50	375826.5
51	378390.0
52	375437.0
53	366985.0
54	357475.5
55	341395.5
56	321096.0
57	297494.0
58	271703.5
59	245838.0
60	212993.5
61	178639.0
62	145884.5
63	116017.5
64	91269.0
65	70515.0
66	54550.5
67	42962.0
68	33543.5
69	24892.0
70	18376.5
71	13566.5
72	9611.0
73	6624.5
74	4704.5
75	3511.5
76	2618.0
77	1900.0
78	1404.0
79	1046.5
80	758.5
81	558.0
82	420.0
83	272.5
84	179.0
85	131.0
86	96.5
87	70.0
88	46.5
89	32.5
90	21.5
91	15.0
92	11.5
93	7.0
94	3.0
95	1.5
96	1.0
97	1.5
98	1.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	5.484784973089545E-4
3	0.003804340215377003
4	0.0034425778022583314
5	4.667902104757059E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.2836730788081912E-4
12-13	0.0017562981669148434
14-15	0.003197512941758585
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0063921055566462485
26-27	0.01982833291284771
28-29	0.008898585377492088
30-31	0.0
32-33	0.0
34-35	1.2461659627213605E-4
36-37	0.014198883601595429
38-39	0.03884168478568446
40-41	0.04443283874712554
42-43	0.00780973351500984
44-45	2.5075981753737106E-4
46-47	6.167577268333154E-6
48-49	0.041533245362058796
50-51	0.055309146218237126
52-53	0.059168510076544105
54-55	0.03602074972751381
56-57	0.001533189332784111
58-59	0.006454564855718896
60-61	0.02955093298140662
62-63	0.04150091645404396
64-65	0.018997165778375097
66-67	0.04828448778715624
68-69	0.06437701413072774
70-71	0.07374107026917387
72-73	0.049773971947938436
74-75	0.023350017100838727
76-77	0.0056754889992302006
78-79	4.823031105894471E-4
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.002425688346640076
92-93	0.002744077000215909
94-95	5.897380092933016E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2296.0
20-21	7020.0
22-23	7257.0
24-25	12760.0
26-27	18219.0
28-29	28881.0
30-31	27380.0
32-33	30030.0
34-35	37603.0
36-37	54499.0
38-39	48005.0
40-41	48802.0
42-43	54995.0
44-45	65753.0
46-47	66426.0
48-49	50155.0
50-51	62773.0
52-53	48345.0
54-55	55944.0
56-57	60710.0
58-59	69314.0
60-61	52501.0
62-63	58778.0
64-65	55623.0
66-67	79611.0
68-69	60162.0
70-71	68501.0
72-73	64880.0
74-75	55213.0
76-77	44267.0
78-79	74270.0
80-81	74750.0
82-83	90387.0
84-85	113785.0
86-87	126244.0
88-89	141106.0
90-91	153067.0
92-93	163982.0
94-95	189646.0
96-97	365837.0
98-99	553690.0
100-101	5125693.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.91177550690965
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.83966980700345	75.38606204460162
2	6.629521189840886	11.12589787600458
3	1.8918954296561292	4.762569137275605
4	0.726383929652877	2.4380866094743627
5	0.3247505002109246	1.362519553472786
6	0.19337176016559748	0.9735700637034954
7	0.11819719037616946	0.6942695273074816
8	0.07361003962078268	0.49413992957710723
9	0.0447753741063435	0.33814630262244516
>10	0.15325756239884722	1.9765711184219559
>50	0.003332359171603057	0.1959759582175143
>100	0.0011131723940773636	0.15447416362643018
>500	7.301125111218052E-5	0.0485219072593681
>1k	4.8674151203415066E-5	0.04919580843511292
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	1.1669755261892648E-5	0.0	0.0	0.0
2	0.0	7.001853157135588E-5	0.0	0.0	0.0
3	0.0	7.001853157135588E-5	0.0	0.0	0.0
4	0.0	1.9838583945217501E-4	0.0	0.0	0.0
5	0.0	2.1005559471406766E-4	0.0	0.0	0.0
6	0.0	2.1005559471406766E-4	0.0	0.0	1.1669755261892648E-5
7	0.0	2.1005559471406766E-4	0.0	0.0	1.1669755261892648E-5
8	0.0	2.3339510523785295E-4	0.0	0.0	1.1669755261892648E-5
9	0.0	5.484784973089545E-4	0.0	0.0	1.1669755261892648E-5
10-11	1.1669755261892648E-5	5.718180078327398E-4	0.0	0.0	1.1669755261892648E-5
12-13	1.1669755261892648E-5	5.718180078327398E-4	0.0	0.0	1.1669755261892648E-5
14-15	1.1669755261892648E-5	8.577270117491096E-4	0.0	0.0	1.1669755261892648E-5
16-17	1.1669755261892648E-5	8.635618893800559E-4	0.0	0.0	2.3339510523785296E-5
18-19	1.1669755261892648E-5	9.627548091061433E-4	0.0	1.1669755261892648E-5	2.917438815473162E-5
20-21	1.1669755261892648E-5	0.0010619477288322308	0.0	2.917438815473162E-5	4.084414341662427E-5
22-23	1.1669755261892648E-5	0.001172810403820211	0.0	4.667902104757059E-5	5.834877630946324E-5
24-25	1.1669755261892648E-5	0.0013245172222248155	0.0	8.168828683324853E-5	8.168828683324853E-5
26-27	1.1669755261892648E-5	0.001388700876165225	0.0	1.8088120655933602E-4	8.168828683324853E-5
28-29	1.1669755261892648E-5	0.0013945357537961714	0.0	5.718180078327398E-4	8.168828683324853E-5
30-31	1.1669755261892648E-5	0.0015637472050936147	0.0	0.001767967922176736	8.752316446419486E-5
32-33	1.1669755261892648E-5	0.0015870867156174001	0.0	0.004422837244257314	1.0502779735703383E-4
34-35	2.3339510523785296E-5	0.0018788305971647163	0.0	0.008291361113574727	1.1669755261892647E-4
36-37	2.3339510523785296E-5	0.0021705744787120325	0.0	0.014283780440556601	1.1669755261892647E-4
38-39	2.3339510523785296E-5	0.0022289232550214955	0.0	0.025288359652521367	1.1669755261892647E-4
40-41	2.3339510523785296E-5	0.0025206671365688117	0.0	0.03672471980917616	1.1669755261892647E-4
42-43	2.3339510523785296E-5	0.002631529811556792	0.0	0.04733252734223658	1.1669755261892647E-4
44-45	2.3339510523785296E-5	0.002958282958889786	0.0	0.059282356730414654	1.225324302498728E-4
46-47	2.3339510523785296E-5	0.003185843186496693	0.0	0.0700710454700344	1.3420218551176544E-4
48-49	2.3339510523785296E-5	0.0031975129417585856	0.0	0.07908593140984647	1.517068184046044E-4
50-51	2.3339510523785296E-5	0.003658468274603345	0.0	0.08725476009317132	1.517068184046044E-4
52-53	2.3339510523785296E-5	0.0037051472956509154	0.0	0.09545276316465091	1.6337657366649708E-4
54-55	2.3339510523785296E-5	0.003716817050912808	0.0	0.10204617488762026	1.7504632892838972E-4
56-57	2.3339510523785296E-5	0.003781000704853218	0.0	0.10772934570016197	1.7504632892838972E-4
58-59	2.3339510523785296E-5	0.003781000704853218	0.0	0.11264814754304972	1.8671608419028237E-4
60-61	2.3339510523785296E-5	0.003839349481162681	0.0	0.1166625433531408	1.9838583945217501E-4
62-63	2.3339510523785296E-5	0.0039210377679959295	0.0	0.1214996569091953	2.1005559471406766E-4
64-65	2.3339510523785296E-5	0.0043178094469002795	0.0	0.1256424200271672	2.1005559471406766E-4
66-67	2.3339510523785296E-5	0.00436448846794785	0.0	0.12961597169384165	2.1005559471406766E-4
68-69	2.3339510523785296E-5	0.004469516265304884	0.0	0.13452893865909843	2.1005559471406766E-4
70-71	2.3339510523785296E-5	0.00453953479687624	0.0	0.13797151646135677	2.2756022760690663E-4
72-73	2.3339510523785296E-5	0.0045570394297690785	0.0	0.14304786000028008	2.5673461576163824E-4
74-75	2.3339510523785296E-5	0.004662067227126113	0.0	0.1470272465445855	2.5673461576163824E-4
76-77	2.3339510523785296E-5	0.004819608923161663	0.0	0.15015474095477271	2.5673461576163824E-4
78-79	2.3339510523785296E-5	0.004901297209994912	0.0	0.1523486549440085	2.5673461576163824E-4
80-81	2.3339510523785296E-5	0.004901297209994912	0.0	0.15327056560969804	2.684043710235309E-4
82-83	2.3339510523785296E-5	0.004942141353411537	0.0	0.1548809918358392	2.800741262854235E-4
84-85	2.3339510523785296E-5	0.004977150619197214	0.0	0.15629886710015917	2.800741262854235E-4
86-87	2.3339510523785296E-5	0.005006325007351946	0.0	0.15701655704876558	2.9174388154731617E-4
88	2.3339510523785296E-5	0.005251389867851691	0.0	0.15709824533559882	2.9174388154731617E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11100	0.0	52.88663	1
AATCTCG	8125	0.0	43.43146	6
CTCGTAT	8545	0.0	41.547657	9
GTATCAA	21360	0.0	40.542206	1
TCTCGTA	8690	0.0	40.365917	8
ATCTCGT	9020	0.0	39.075165	7
TCAACGC	27600	0.0	30.945753	4
TATCAAC	27865	0.0	30.819904	2
ATCAACG	27755	0.0	30.698236	3
CAACGCA	27835	0.0	30.637693	5
GAAATCT	12000	0.0	29.759066	4
AAATCTC	12085	0.0	29.72339	5
AACGCAG	28765	0.0	29.721642	6
GTACATG	38995	0.0	26.315184	1
TACATGG	38320	0.0	26.260351	2
ACGCAGA	33480	0.0	25.397083	7
ACATGGG	39075	0.0	25.298532	3
CGCAGAG	34495	0.0	24.626144	8
CATGGGG	30230	0.0	22.927124	4
GCAGAAA	17280	0.0	21.890009	1
>>END_MODULE