FastQCFastQC Report
Fri 27 May 2016
SRR2139343_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139343_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8881018
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101050.046.641541
GTATCAA180750.036.877081
TCAACGC215150.030.2091714
ATCAACG218000.029.7960593
CAACGCA220000.029.5043535
AACGCAG224900.028.9171436
TATCAAC229400.028.5142772
ACGCAGA263650.024.5728517
CGCAGAG267500.024.2817128
GTACATG248050.023.7968541
TACATGG249300.022.9791812
ACATGGG254100.021.9087543
GCAGAGT320250.020.2182279
CATGGGG191550.020.1682324
GAGTACT182950.019.20231612-13
AGAGTAC279300.018.24772810-11
CAGAGTA305950.017.93183310-11
GTACTTT209950.016.75169214-15
ATGGGGG117700.016.0585145
AGTACTT204600.015.91427512-13
Produced by FastQC (version 0.11.5)