##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139343_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8881018
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.578319962869124	33.0	33.0	33.0	33.0	33.0
2	32.58455663528663	33.0	33.0	33.0	33.0	33.0
3	32.641541093599855	33.0	33.0	33.0	33.0	33.0
4	36.54999967346086	37.0	37.0	37.0	37.0	37.0
5	36.57408756518679	37.0	37.0	37.0	37.0	37.0
6	36.11216169137367	37.0	37.0	37.0	33.0	37.0
7	35.964863600096294	37.0	37.0	37.0	33.0	37.0
8	35.86718741027211	37.0	37.0	37.0	33.0	37.0
9	35.86864546384209	37.0	37.0	37.0	33.0	37.0
10-11	35.80000322035154	37.0	37.0	37.0	33.0	37.0
12-13	35.711280846407476	37.0	37.0	37.0	33.0	37.0
14-15	37.36675401401055	40.0	37.0	40.0	33.0	40.0
16-17	37.2963733436865	40.0	37.0	40.0	33.0	40.0
18-19	37.320230011919804	40.0	37.0	40.0	33.0	40.0
20-21	37.23221375908854	40.0	37.0	40.0	33.0	40.0
22-23	37.108242345349474	40.0	37.0	40.0	33.0	40.0
24-25	37.09436803370665	40.0	37.0	40.0	33.0	40.0
26-27	36.93497182757936	40.0	37.0	40.0	33.0	40.0
28-29	36.76433175107357	40.0	37.0	40.0	33.0	40.0
30-31	36.57026087000899	40.0	37.0	40.0	33.0	40.0
32-33	36.351212619969715	38.5	37.0	40.0	33.0	40.0
34-35	36.24999799015694	37.0	37.0	40.0	33.0	40.0
36-37	36.061457110407616	37.0	37.0	40.0	30.0	40.0
38-39	36.0976243127832	37.0	37.0	40.0	27.0	40.0
40-41	36.13905914783729	37.0	37.0	40.0	27.0	40.0
42-43	36.04956349862552	37.0	37.0	40.0	27.0	40.0
44-45	35.919797732021806	37.0	37.0	40.0	27.0	40.0
46-47	35.747500132407644	37.0	37.0	40.0	27.0	40.0
48-49	35.55671469198788	37.0	37.0	40.0	27.0	40.0
50-51	35.549538328034345	37.0	35.0	40.0	27.0	40.0
52-53	35.43692684923915	37.0	33.0	40.0	27.0	40.0
54-55	35.24896733106847	37.0	33.0	40.0	27.0	40.0
56-57	35.00824855657905	37.0	33.0	40.0	27.0	40.0
58-59	34.88161797832885	37.0	33.0	40.0	27.0	40.0
60-61	34.754539793874486	37.0	33.0	40.0	27.0	40.0
62-63	34.58432763073024	37.0	33.0	40.0	27.0	40.0
64-65	34.40184183645935	37.0	33.0	40.0	27.0	40.0
66-67	34.14462819901783	37.0	33.0	37.0	27.0	40.0
68-69	33.9411578318995	37.0	33.0	37.0	27.0	40.0
70-71	33.701718846600286	37.0	33.0	37.0	27.0	40.0
72-73	33.39710676869747	37.0	33.0	37.0	22.0	40.0
74-75	33.04217059678258	37.0	33.0	37.0	22.0	38.5
76-77	31.005397533009027	33.0	30.0	35.0	22.0	37.0
78-79	33.09646298445931	35.0	33.0	37.0	27.0	37.0
80-81	33.45348556734986	37.0	33.0	37.0	27.0	37.0
82-83	33.39150438334785	37.0	33.0	37.0	27.0	37.0
84-85	33.28396138890311	37.0	33.0	37.0	27.0	37.0
86-87	33.13904313058765	35.0	33.0	37.0	27.0	37.0
88-89	32.97455510935788	33.0	33.0	37.0	27.0	37.0
90-91	32.89348115814077	33.0	33.0	37.0	27.0	37.0
92-93	32.939904873265895	33.0	33.0	37.0	27.0	37.0
94-95	32.9945759742439	33.0	33.0	37.0	27.0	37.0
96-97	33.116101192892735	33.0	33.0	37.0	27.0	37.0
98-99	33.27710109486266	33.0	33.0	37.0	27.0	37.0
100	33.24754239470685	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	3.0
15	12.0
16	44.0
17	113.0
18	429.0
19	1102.0
20	2769.0
21	7521.0
22	18897.0
23	41780.0
24	77941.0
25	127790.0
26	182297.0
27	238609.0
28	283261.0
29	318224.0
30	341818.0
31	362140.0
32	390077.0
33	433988.0
34	516022.0
35	673479.0
36	1063631.0
37	2098121.0
38	1661896.0
39	39052.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.643771243341696	20.111782230370437	13.474817864348434	23.769628661939432
2	14.997195694577641	21.37618262474655	41.019100692299176	22.60752098837663
3	18.296039201374576	26.69777829675536	30.327477231829125	24.67870527004094
4	12.446064333304545	17.945134847752755	38.98041363542749	30.62838718351521
5	13.188605131282854	38.371852766829015	35.55055362478276	12.888988477105375
6	30.2381551304141	38.01301832740346	18.744168742817546	13.004657799364894
7	26.813964345078457	32.735154911295076	23.21659521464769	17.234285528978774
8	25.05624918224465	36.56132664070718	21.16096375438041	17.22146042266776
9	26.303673745509805	16.439376657045397	20.781164952035905	36.47578464540889
10-11	24.519281682768725	26.619972634858446	29.12767826484532	19.733067417527504
12-13	25.505328085195316	24.189724107924633	28.927557048503388	21.377390758376663
14-15	22.582367084154775	25.552054718011984	26.367224008111044	25.4983541897222
16-17	22.182614650707837	28.600414952430004	27.04456290934215	22.17240748752001
18-19	22.202437828636313	27.706998229257053	28.726453431352127	21.36411051075451
20-21	23.032807786659404	26.996232158008393	28.482527562881437	21.48843249245077
22-23	22.692637204223594	27.03235141789062	28.333396379382826	21.941614998502963
24-25	22.497634868918677	27.156751179178716	28.453435705689635	21.892178246212968
26-27	22.487682720128706	27.308721436201676	28.353279259061587	21.850316584608027
28-29	22.339036852364455	27.334124315486395	28.375456214781714	21.951382617367432
30-31	22.653898166271773	27.202760368853458	28.274950428857814	21.868391036016956
32-33	22.425824594748796	27.448632200444216	28.203707585192276	21.92183561961471
34-35	22.54072256420791	27.4726460195171	28.218396203528684	21.768235212746298
36-37	22.603735214602644	27.37786948279232	28.094871352750907	21.92352394985413
38-39	22.41031276299358	27.55338329828828	28.18653441587325	21.849769522844895
40-41	22.597708812052524	27.389533757268385	28.098701270255628	21.914056160423467
42-43	22.508548850545218	27.460386547693123	27.951983210745507	22.07908139101615
44-45	22.530947380393716	27.595961131796333	27.719443838335316	22.15364764947464
46-47	22.673579875912797	27.5212899509712	27.437063869733514	22.368066303382488
48-49	22.527138423365734	27.62475235199669	27.5381083294426	22.310000895194975
50-51	22.531201351616502	27.760402592518478	27.45107642528693	22.25731963057809
52-53	22.676565477388795	27.69394107548724	27.351382360506634	22.278111086617333
54-55	22.594172531592434	27.64027402661361	27.361786416854176	22.403767024939775
56-57	22.577561917001454	27.81317208994794	27.287485185792075	22.321780807258527
58-59	22.50193316553962	27.908068502289474	27.32535807785224	22.264640254318664
60-61	22.605849651074184	27.90961663860525	27.286003863360627	22.19852984695994
62-63	22.572243265152686	28.02250660664422	27.2292697833817	22.17598034482139
64-65	22.586083428016206	27.925550442629632	27.226182427111617	22.26218370224255
66-67	22.6191775356812	27.854969044787335	27.19268900616723	22.33316441336423
68-69	22.498832044911605	27.885444929628783	27.29111680608705	22.324606219372562
70-71	22.563991440978285	27.62079761856167	27.34431974440723	22.470891196052815
72-73	22.57622534634541	27.69787503009996	27.24578886254622	22.480110761008408
74-75	22.42911185682915	27.790159778075935	27.2780693635844	22.502659001510512
76-77	22.540732722883625	27.71355371654603	27.314473776140513	22.43123978442983
78-79	22.418425840335896	27.74314131582193	27.31281884904898	22.525613994793193
80-81	22.4475861304672	27.790259163393692	27.296012073561794	22.466142632577313
82-83	22.467299907763223	27.72408903696157	27.40305022234597	22.405560832929236
84-85	22.536440363643948	27.646705034332943	27.383927813940396	22.432926788082714
86-87	22.364869042288515	27.693161763643104	27.468500021907698	22.47346917216068
88-89	22.42787498033595	27.62576196975196	27.45418711824209	22.492175931669998
90-91	22.45403308121351	27.6155618630781	27.47624539508046	22.45415966062793
92-93	22.441896191068757	27.63330821246366	27.496367155532475	22.428428440935114
94-95	22.3311232342431	27.659386772687267	27.549525065945428	22.459964927124204
96-97	22.37188041978837	27.73807740868089	27.61496834815409	22.27507382337666
98-99	23.477540677777426	29.667631198122958	27.92784068480551	18.92698743929411
100	22.570118931847556	30.506437301005057	21.703335762503524	25.22010800464386
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1003.0
1	1013.5
2	1689.0
3	2542.5
4	3606.0
5	6584.0
6	9329.5
7	10480.0
8	10440.0
9	9388.5
10	8276.5
11	7398.0
12	7779.5
13	10760.0
14	17979.0
15	28426.0
16	38132.0
17	44953.0
18	48054.0
19	46864.0
20	43698.5
21	40371.0
22	38192.0
23	39216.0
24	43618.5
25	52153.5
26	64181.0
27	78020.0
28	94349.5
29	113091.5
30	132598.0
31	153844.0
32	176786.5
33	198802.0
34	221232.5
35	243391.0
36	266483.5
37	289205.0
38	307359.5
39	322212.5
40	338059.5
41	352325.5
42	362722.0
43	373777.0
44	383587.5
45	392252.5
46	396621.5
47	396422.5
48	395230.0
49	389726.5
50	382726.5
51	372205.5
52	355809.5
53	336981.5
54	316643.0
55	294786.5
56	272009.5
57	247541.5
58	221111.0
59	194278.5
60	166017.5
61	139208.5
62	114872.0
63	92322.0
64	72361.0
65	56056.0
66	43514.0
67	34613.0
68	27977.0
69	21811.0
70	16644.5
71	12743.0
72	9641.5
73	7077.5
74	5245.5
75	3962.0
76	2990.0
77	2198.0
78	1627.0
79	1206.5
80	868.5
81	621.5
82	458.5
83	309.5
84	210.5
85	160.0
86	124.5
87	95.0
88	66.0
89	47.5
90	33.5
91	23.0
92	18.0
93	13.0
94	7.5
95	6.0
96	6.0
97	5.0
98	2.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	3.4905908309160053E-4
3	0.003479330860493696
4	0.003366731156270599
5	3.3779911266929086E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.294896598565615E-4
12-13	0.001734035445035693
14-15	0.0032203515407805724
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.006417616849309107
26-27	0.02022822664956042
28-29	0.008847568977292488
30-31	0.0
32-33	0.0
34-35	2.2366949769164475E-4
36-37	0.013541073764509548
38-39	0.037740688810997486
40-41	0.042861733730053295
42-43	0.00784691317507865
44-45	3.606098207650958E-4
46-47	1.7910277747214218E-5
48-49	0.03336125289342279
50-51	0.05456183126607923
52-53	0.05285776579959793
54-55	0.028975091003855532
56-57	0.0013118730066948628
58-59	0.006165533455094197
60-61	0.028741316901836834
62-63	0.034238244293329806
64-65	0.01682508051699454
66-67	0.04430036267327203
68-69	0.061202331750628225
70-71	0.0739218640215158
72-73	0.05154792883609058
74-75	0.023592336052803673
76-77	0.005105120372794072
78-79	4.242816981563829E-4
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0022862884006463313
92-93	0.002807054663819913
94-95	6.181823467505078E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2429.0
20-21	7525.0
22-23	9093.0
24-25	15569.0
26-27	21324.0
28-29	30363.0
30-31	31827.0
32-33	34503.0
34-35	40387.0
36-37	41736.0
38-39	45951.0
40-41	58483.0
42-43	65087.0
44-45	79602.0
46-47	83048.0
48-49	63608.0
50-51	72550.0
52-53	68775.0
54-55	83233.0
56-57	87069.0
58-59	89467.0
60-61	90712.0
62-63	85731.0
64-65	82598.0
66-67	96308.0
68-69	79057.0
70-71	85776.0
72-73	80640.0
74-75	80187.0
76-77	73861.0
78-79	96946.0
80-81	110578.0
82-83	119292.0
84-85	126372.0
86-87	137847.0
88-89	144309.0
90-91	155150.0
92-93	171648.0
94-95	198447.0
96-97	371299.0
98-99	539139.0
100-101	4923492.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.8884590367134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.49462943584817	79.49827361815966
2	5.77458620180194	10.034897932984766
3	1.4680065359897356	3.8265847730391505
4	0.5627717064355005	1.9559346544656913
5	0.2631522011019573	1.1432444622934193
6	0.15128299696851474	0.7886847891030018
7	0.08359438874628061	0.5084371335596207
8	0.05096949808641661	0.35429289172827616
9	0.03342639175164888	0.26139309034124514
>10	0.1156955794154254	1.496548951242104
>50	0.001661683051942372	0.09516135025523009
>100	2.2338080242478888E-4	0.03654635282785718
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1259970422309694E-5
2	0.0	2.2519940844619388E-5	0.0	0.0	1.1259970422309694E-5
3	0.0	2.2519940844619388E-5	0.0	0.0	1.1259970422309694E-5
4	0.0	1.2385967464540663E-4	0.0	0.0	1.1259970422309694E-5
5	0.0	1.2385967464540663E-4	0.0	0.0	1.1259970422309694E-5
6	1.1259970422309694E-5	1.2385967464540663E-4	0.0	0.0	2.2519940844619388E-5
7	1.1259970422309694E-5	1.2385967464540663E-4	0.0	0.0	2.2519940844619388E-5
8	1.1259970422309694E-5	1.4637961549002603E-4	0.0	1.1259970422309694E-5	2.2519940844619388E-5
9	1.1259970422309694E-5	2.8149926055774237E-4	0.0	1.1259970422309694E-5	2.2519940844619388E-5
10-11	1.1259970422309694E-5	2.8149926055774237E-4	0.0	1.1259970422309694E-5	2.2519940844619388E-5
12-13	1.1259970422309694E-5	2.8149926055774237E-4	0.0	1.688995563346454E-5	2.2519940844619388E-5
14-15	1.1259970422309694E-5	3.884689795696845E-4	0.0	2.2519940844619388E-5	3.3779911266929086E-5
16-17	1.1259970422309694E-5	3.940989647808393E-4	0.0	2.2519940844619388E-5	4.5039881689238777E-5
18-19	1.1259970422309694E-5	4.447688316812329E-4	0.0	2.8149926055774237E-5	4.5039881689238777E-5
20-21	1.1259970422309694E-5	5.123286542150911E-4	0.0	6.755982253385817E-5	5.6299852111548474E-5
22-23	1.688995563346454E-5	5.911484471712589E-4	0.0	7.318980774501303E-5	6.755982253385817E-5
24-25	2.2519940844619388E-5	6.418183140716526E-4	0.0	1.0133973380078725E-4	6.755982253385817E-5
26-27	2.2519940844619388E-5	6.418183140716526E-4	0.0	1.294896598565615E-4	6.755982253385817E-5
28-29	2.2519940844619388E-5	6.418183140716526E-4	0.0	3.772090091473748E-4	6.755982253385817E-5
30-31	2.2519940844619388E-5	6.868581957608914E-4	0.0	9.90877397163253E-4	6.755982253385817E-5
32-33	2.2519940844619388E-5	6.868581957608914E-4	0.0	0.002139394380238842	1.0133973380078725E-4
34-35	3.3779911266929086E-5	8.10717870406298E-4	0.0	0.003732680194995664	1.1259970422309695E-4
36-37	3.3779911266929086E-5	9.23317574629395E-4	0.0	0.006508262904095004	1.1259970422309695E-4
38-39	3.3779911266929086E-5	9.57097485896324E-4	0.0	0.01141761000822203	1.1259970422309695E-4
40-41	3.3779911266929086E-5	0.0010246573084301822	0.0	0.01701381530810995	1.2385967464540663E-4
42-43	3.3779911266929086E-5	0.001069697190119421	0.0	0.02250868087419708	1.2948965985656147E-4
44-45	3.3779911266929086E-5	0.0012217067908206018	0.0	0.02933785293532791	1.4074963027887119E-4
46-47	3.3779911266929086E-5	0.0013174165394102342	0.0	0.03665120372461805	1.6326957112349058E-4
48-49	3.3779911266929086E-5	0.0013174165394102342	0.0	0.04289485732378878	1.6889955633464542E-4
50-51	3.3779911266929086E-5	0.00147505612532257	0.0	0.04873315198775636	1.6889955633464542E-4
52-53	3.3779911266929086E-5	0.0014863160957448798	0.0	0.05493176570523785	1.8578951196810995E-4
54-55	3.3779911266929086E-5	0.0014863160957448798	0.0	0.061901687396647545	2.026794676015745E-4
56-57	3.3779911266929086E-5	0.0015088360365894992	0.0	0.06842684025637602	2.026794676015745E-4
58-59	3.3779911266929086E-5	0.0015088360365894992	0.0	0.07387666594077391	2.026794676015745E-4
60-61	5.0669866900393625E-5	0.001520096007011809	0.0	0.07952917109277338	2.0830945281272937E-4
62-63	5.6299852111548474E-5	0.0015651358887010475	0.0	0.0855982951503983	2.139394380238842E-4
64-65	5.6299852111548474E-5	0.0016889955633464541	0.0	0.09096367105662886	2.139394380238842E-4
66-67	6.192983732270332E-5	0.0017227754746133833	0.0	0.09686389555791915	2.251994084461939E-4
68-69	6.755982253385817E-5	0.0017396654302468477	0.0	0.10316384900920142	2.251994084461939E-4
70-71	6.755982253385817E-5	0.0017509254006691575	0.0	0.10776917691192608	2.251994084461939E-4
72-73	7.318980774501303E-5	0.0017565553858803124	0.0	0.11414232017095338	3.0401920140236174E-4
74-75	7.881979295616787E-5	0.0017678153563026222	0.0	0.11933316653563814	3.0401920140236174E-4
76-77	7.881979295616787E-5	0.001812855237991861	0.0	0.12406235411300821	3.096491866135166E-4
78-79	7.881979295616787E-5	0.00184663514925879	0.0	0.12668592722140637	3.152791718246715E-4
80-81	7.881979295616787E-5	0.00184663514925879	0.0	0.12761487478124695	3.2653914224698116E-4
82-83	7.881979295616787E-5	0.00184663514925879	0.0	0.129799309043175	3.4905908309160053E-4
84-85	7.881979295616787E-5	0.0018522651344699448	0.0	0.13150519456215493	3.4905908309160053E-4
86-87	7.881979295616787E-5	0.0019310849274261125	0.0	0.1324285121367843	3.4905908309160053E-4
88	7.881979295616787E-5	0.0020155347055934355	0.0	0.1325298518705851	3.4905908309160053E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10105	0.0	46.64154	1
GTATCAA	18075	0.0	36.87708	1
TCAACGC	21515	0.0	30.209171	4
ATCAACG	21800	0.0	29.796059	3
CAACGCA	22000	0.0	29.504353	5
AACGCAG	22490	0.0	28.917143	6
TATCAAC	22940	0.0	28.514277	2
ACGCAGA	26365	0.0	24.572851	7
CGCAGAG	26750	0.0	24.281712	8
GTACATG	24805	0.0	23.796854	1
TACATGG	24930	0.0	22.979181	2
ACATGGG	25410	0.0	21.908754	3
GCAGAGT	32025	0.0	20.218227	9
CATGGGG	19155	0.0	20.168232	4
GAGTACT	18295	0.0	19.202316	12-13
AGAGTAC	27930	0.0	18.247728	10-11
CAGAGTA	30595	0.0	17.931833	10-11
GTACTTT	20995	0.0	16.751692	14-15
ATGGGGG	11770	0.0	16.058514	5
AGTACTT	20460	0.0	15.914275	12-13
>>END_MODULE