FastQCFastQC Report
Fri 27 May 2016
SRR2139342_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139342_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12293410
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA146600.048.8425331
GTATCAA270200.037.0757681
TCAACGC332200.029.4009284
ATCAACG334300.029.2541523
CAACGCA339550.028.690915
TATCAAC347600.028.3712792
AACGCAG348850.028.020646
ACGCAGA400900.024.1368687
CGCAGAG407050.023.8027388
GTACATG357000.022.7020241
TACATGG360500.022.253842
ACATGGG363750.021.5017663
GCAGAGT478200.020.0390429
CATGGGG279800.019.4857254
GAGTACT279450.018.69536812-13
AGAGTAC426950.018.31907810-11
GTGGTAT68250.017.7461851
CAGAGTA465050.017.71876110-11
TGGTATC67650.016.5683982
GTACTTT318750.016.37884714-15
Produced by FastQC (version 0.11.5)