##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139342_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12293410
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5473015217096	33.0	33.0	33.0	33.0	33.0
2	32.55855055676171	33.0	33.0	33.0	33.0	33.0
3	32.602120323002325	33.0	33.0	33.0	33.0	33.0
4	36.501686431998934	37.0	37.0	37.0	37.0	37.0
5	36.526462551887555	37.0	37.0	37.0	37.0	37.0
6	36.111038759790816	37.0	37.0	37.0	33.0	37.0
7	35.97604204203716	37.0	37.0	37.0	33.0	37.0
8	35.89244172284175	37.0	37.0	37.0	33.0	37.0
9	35.89797167750852	37.0	37.0	37.0	33.0	37.0
10-11	35.82815301043404	37.0	37.0	37.0	33.0	37.0
12-13	35.73736257881255	37.0	37.0	37.0	33.0	37.0
14-15	37.31782800703792	40.0	37.0	40.0	33.0	40.0
16-17	37.2533441494264	40.0	37.0	40.0	33.0	40.0
18-19	37.286678635138664	40.0	37.0	40.0	33.0	40.0
20-21	37.19420153248209	40.0	37.0	40.0	33.0	40.0
22-23	37.07581393587053	40.0	37.0	40.0	33.0	40.0
24-25	37.05957022336172	40.0	37.0	40.0	33.0	40.0
26-27	36.899636440423855	40.0	37.0	40.0	33.0	40.0
28-29	36.74213969749039	40.0	37.0	40.0	33.0	40.0
30-31	36.542777436890766	37.0	37.0	40.0	33.0	40.0
32-33	36.306490484154	37.0	37.0	40.0	33.0	40.0
34-35	36.192691441440346	37.0	37.0	40.0	33.0	40.0
36-37	36.02340800806321	37.0	37.0	40.0	30.0	40.0
38-39	36.04083698211924	37.0	37.0	40.0	30.0	40.0
40-41	36.05308063757488	37.0	37.0	40.0	30.0	40.0
42-43	35.9507385471248	37.0	37.0	40.0	27.0	40.0
44-45	35.821447438377625	37.0	37.0	40.0	27.0	40.0
46-47	35.63949017415498	37.0	37.0	40.0	27.0	40.0
48-49	35.41226291972592	37.0	33.0	40.0	27.0	40.0
50-51	35.37962079074559	37.0	33.0	40.0	27.0	40.0
52-53	35.253868504186	37.0	33.0	40.0	27.0	40.0
54-55	35.039071124474916	37.0	33.0	40.0	27.0	40.0
56-57	34.80088753174886	37.0	33.0	40.0	27.0	40.0
58-59	34.67683983864727	37.0	33.0	40.0	27.0	40.0
60-61	34.54463622837618	37.0	33.0	40.0	27.0	40.0
62-63	34.366441221946964	37.0	33.0	40.0	27.0	40.0
64-65	34.15119441015116	37.0	33.0	38.5	27.0	40.0
66-67	33.859914907797716	37.0	33.0	37.0	27.0	40.0
68-69	33.62460669516686	37.0	33.0	37.0	27.0	40.0
70-71	33.3759386647242	37.0	33.0	37.0	24.5	40.0
72-73	33.07771474337998	37.0	33.0	37.0	22.0	40.0
74-75	32.724299743289365	37.0	33.0	37.0	22.0	37.0
76-77	30.62605309697931	33.0	27.0	35.0	22.0	37.0
78-79	32.76244947580447	33.0	33.0	37.0	27.0	37.0
80-81	33.140522665283555	35.0	33.0	37.0	27.0	37.0
82-83	33.08991852490434	33.0	33.0	37.0	27.0	37.0
84-85	32.9772787493667	33.0	33.0	37.0	27.0	37.0
86-87	32.80776329790336	33.0	33.0	37.0	27.0	37.0
88-89	32.61289546853229	33.0	33.0	37.0	27.0	37.0
90-91	32.49373853750781	33.0	33.0	37.0	27.0	37.0
92-93	32.51867649623735	33.0	33.0	37.0	27.0	37.0
94-95	32.561346162998646	33.0	33.0	37.0	27.0	37.0
96-97	32.66221360567276	33.0	33.0	37.0	27.0	37.0
98-99	32.82231516001603	33.0	33.0	37.0	27.0	37.0
100	32.796127409225384	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	3.0
14	5.0
15	9.0
16	33.0
17	148.0
18	446.0
19	1307.0
20	3263.0
21	9067.0
22	22715.0
23	50980.0
24	96681.0
25	159743.0
26	231155.0
27	307589.0
28	373161.0
29	428359.0
30	472945.0
31	517332.0
32	576370.0
33	670232.0
34	829048.0
35	1133910.0
36	1791520.0
37	2924184.0
38	1664958.0
39	28246.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.14141560396993	20.41939543218684	13.858579515366362	23.580609448476867
2	14.941954087129227	21.53440894817142	41.08152750929525	22.4421094554041
3	18.081401378910904	26.902746148530447	30.460126404911396	24.55572606764725
4	12.504337857829547	18.248572716651694	39.018325486820316	30.228763938698442
5	13.22083454977127	38.24171769469603	35.543255014659486	12.994192740873217
6	29.810679054875745	38.12512557540991	18.929369475190367	13.134825894523978
7	26.503671479272228	32.75923441909121	23.50104649564279	17.236047605993782
8	24.81025199680154	36.51153748227709	21.276464382136446	17.401746138784926
9	26.14062330956179	16.525992381283956	21.13739800429661	36.19598630485764
10-11	24.388272390539946	26.705080502339445	29.045441901937547	19.861205205183058
12-13	25.349920012937194	24.297060709310273	29.034030741127765	21.31898853662477
14-15	22.457072201512645	25.538079520703818	26.5829972687764	25.421851009007142
16-17	22.017564695231023	28.59112321154179	27.228535451107543	22.16277664211964
18-19	22.064085554780977	27.703375222985322	28.973344255174116	21.259194967059585
20-21	22.832401083916444	26.974185408567923	28.739655376290834	21.45375813122479
22-23	22.629169692720197	26.912746140626236	28.591284493446988	21.86679967320658
24-25	22.38388135878478	27.074302590155764	28.668581428845407	21.873234622214046
26-27	22.354424973313908	27.225235362925144	28.53634643409585	21.8839932296651
28-29	22.282827323961417	27.213442991482744	28.594130864347285	21.909598820208554
30-31	22.520573704366146	27.168862645337345	28.39904316052327	21.91152048977324
32-33	22.35348571954587	27.334273436354238	28.370769185846918	21.941471658252976
34-35	22.46860450720127	27.34563540870948	28.355826026598763	21.82993405749049
36-37	22.482614372374403	27.33244340165356	28.214052459022405	21.97088976694963
38-39	22.314126822867948	27.42986420404544	28.342772579737236	21.91323639334938
40-41	22.538225123719542	27.289131653821073	28.211132244581634	21.961510977877747
42-43	22.41666729863506	27.386213948937012	28.005318589184103	22.191800163243826
44-45	22.460474468170798	27.53207722078429	27.81410186019309	22.193346450851816
46-47	22.618645903547403	27.515188001392936	27.490544752892244	22.375621342167413
48-49	22.49624909858647	27.610890589524452	27.505194571518714	22.38766574037037
50-51	22.47839526904546	27.756875769293902	27.393726787731154	22.371002173929483
52-53	22.629075161197882	27.669693288062852	27.285184249008253	22.41604730173101
54-55	22.60901005541223	27.567831107065082	27.28059587735635	22.542562960166336
56-57	22.569841227732876	27.716283348193155	27.263732772561415	22.450142651512557
58-59	22.536819651163604	27.76949557178896	27.28729303855294	22.406391738494495
60-61	22.61060137179741	27.76324008615961	27.27004233980329	22.35611620223969
62-63	22.5783560799209	27.87083406350747	27.21888992496534	22.331919931606283
64-65	22.561148000372604	27.793141519849318	27.187831166841526	22.45787931293655
66-67	22.59657979797793	27.77192836466475	27.15632876858633	22.47516306877099
68-69	22.50077314003191	27.754121772017253	27.25829771308133	22.486807374869503
70-71	22.564361358117242	27.574509166930405	27.270972321021347	22.590157153931003
72-73	22.557953841190113	27.594012226113158	27.217341651468885	22.63069228122785
74-75	22.481739042979076	27.707368275417732	27.201848316443783	22.60904436515941
76-77	22.59342786050614	27.60832660835651	27.224467151773883	22.573778379363468
78-79	22.483168029057655	27.603591314986648	27.237774407735643	22.675466248220058
80-81	22.486376027570802	27.664221723954718	27.260428027038643	22.58897422143584
82-83	22.53164757078475	27.571168687546603	27.33676772957364	22.560416012095004
84-85	22.55722165089134	27.55402847657878	27.3195927557843	22.56915711674558
86-87	22.45184833034247	27.556989339439124	27.41739477066984	22.573767559548564
88-89	22.483026881944756	27.51371175886038	27.356439878730797	22.64682148046406
90-91	22.48391920845359	27.50425708587333	27.406734022967207	22.605089682705877
92-93	22.471557106139507	27.465506516610617	27.441934415827106	22.62100196142277
94-95	22.343597109374503	27.44734241264813	27.535710585916767	22.6733498920606
96-97	22.442081701603335	27.54938357764144	27.555281684999365	22.453253035755857
98-99	23.50483682529352	29.46032097108243	27.821293104324923	19.213549099299122
100	22.616478513534066	30.33464512363867	21.579047914433215	25.46982844839405
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2140.0
1	2023.5
2	3006.0
3	4412.0
4	6224.0
5	10895.5
6	14942.5
7	16598.0
8	16507.0
9	14833.5
10	12927.5
11	11430.5
12	12208.0
13	17201.0
14	28696.5
15	45323.0
16	60747.0
17	71278.0
18	76138.0
19	74225.5
20	69276.5
21	64343.0
22	61398.5
23	63726.0
24	71610.5
25	85125.5
26	103946.0
27	125455.0
28	149654.0
29	176764.5
30	202696.0
31	229736.5
32	259162.0
33	287066.0
34	315023.0
35	341399.0
36	367993.0
37	395593.5
38	419011.5
39	437468.0
40	455807.0
41	473879.0
42	487524.5
43	499133.0
44	509676.5
45	520889.5
46	528451.0
47	531751.0
48	533064.5
49	527886.5
50	520173.5
51	510614.0
52	495043.5
53	473035.0
54	448452.5
55	421481.0
56	392631.0
57	360378.0
58	324153.0
59	286390.0
60	244236.0
61	203081.5
62	166851.0
63	134134.0
64	104713.0
65	80491.5
66	62159.5
67	49203.0
68	39303.5
69	30234.5
70	23038.0
71	17839.0
72	13416.5
73	9798.5
74	7252.5
75	5350.0
76	3924.5
77	2863.5
78	2093.0
79	1538.5
80	1110.0
81	791.5
82	601.0
83	426.0
84	301.5
85	224.5
86	179.0
87	142.5
88	95.5
89	58.5
90	37.0
91	25.0
92	18.5
93	9.0
94	2.0
95	2.0
96	1.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	3.09108701328598E-4
3	0.0030504148157427437
4	0.0038719932061161222
5	2.4403318525941947E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	7.72771753321495E-5
12-13	0.0013991235954873384
14-15	0.0031317592108292165
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0066900488153415665
26-27	0.02057506111462923
28-29	0.009352668788543161
30-31	0.0
32-33	0.0
34-35	1.2844743063082563E-4
36-37	0.01065770985730668
38-39	0.030313565721122995
40-41	0.0347447590857545
42-43	0.0061474673904120215
44-45	2.925959960131214E-4
46-47	4.35239269306387E-6
48-49	0.030703771090142277
50-51	0.0548052834124943
52-53	0.051524760050495166
54-55	0.02564428893061772
56-57	0.001032664935981429
58-59	0.0064815081782379465
60-61	0.03044587995896899
62-63	0.032994248474526644
64-65	0.01357931409813324
66-67	0.035724069967253747
68-69	0.05132589937958072
70-71	0.06974555154761239
72-73	0.04951229356911614
74-75	0.02043737305438531
76-77	0.0039400692233498725
78-79	2.2462253749707601E-4
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0016845084183308204
92-93	0.001930051364385477
94-95	3.4367669253406575E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	3143.0
20-21	9769.0
22-23	11406.0
24-25	19999.0
26-27	27312.0
28-29	39377.0
30-31	46347.0
32-33	53226.0
34-35	63608.0
36-37	74799.0
38-39	85228.0
40-41	96142.0
42-43	111206.0
44-45	128761.0
46-47	131121.0
48-49	112904.0
50-51	120375.0
52-53	113187.0
54-55	120169.0
56-57	124932.0
58-59	124859.0
60-61	120496.0
62-63	115962.0
64-65	122309.0
66-67	127191.0
68-69	122612.0
70-71	123246.0
72-73	119080.0
74-75	117253.0
76-77	105376.0
78-79	126116.0
80-81	141362.0
82-83	146800.0
84-85	158172.0
86-87	174567.0
88-89	194304.0
90-91	211468.0
92-93	232963.0
94-95	281362.0
96-97	511705.0
98-99	749111.0
100-101	6674085.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.94130193096751
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.48124127652841	79.53498218842344
2	5.901930060639066	10.262429667549489
3	1.411705185156999	3.682064602207412
4	0.5484056272371006	1.9071639687304953
5	0.2510327714723867	1.0912557989574168
6	0.12935084246093093	0.6747558389652486
7	0.07775275295277885	0.4731947899302031
8	0.04885394203086146	0.3397940259698488
9	0.03352257163265175	0.2623046419635176
>10	0.11287831577007626	1.545556315595524
>50	0.0027710355996195973	0.15495045674566316
>100	5.55618519106311E-4	0.07154770496174875
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.134439508647316E-6
2	0.0	1.6268879017294633E-5	0.0	0.0	8.134439508647316E-6
3	0.0	1.6268879017294633E-5	0.0	0.0	8.134439508647316E-6
4	0.0	5.694107656053121E-5	0.0	0.0	8.134439508647316E-6
5	0.0	8.947883459512047E-5	0.0	0.0	8.134439508647316E-6
6	5.694107656053121E-5	8.947883459512047E-5	0.0	0.0	1.6268879017294633E-5
7	5.694107656053121E-5	8.947883459512047E-5	0.0	0.0	1.6268879017294633E-5
8	5.694107656053121E-5	9.761327410376779E-5	0.0	0.0	1.6268879017294633E-5
9	5.694107656053121E-5	1.8709210869888827E-4	0.0	0.0	2.4403318525941947E-5
10-11	6.100829631485487E-5	1.8709210869888827E-4	0.0	0.0	2.4403318525941947E-5
12-13	6.507551606917853E-5	1.9115932845321194E-4	0.0	0.0	2.4403318525941947E-5
14-15	6.507551606917853E-5	2.6436928403103776E-4	0.0	0.0	2.4403318525941947E-5
16-17	9.761327410376779E-5	2.8470538280265606E-4	0.0	8.134439508647316E-6	2.4403318525941947E-5
18-19	9.761327410376779E-5	3.131759210829217E-4	0.0	1.6268879017294633E-5	3.6604977788912924E-5
20-21	9.761327410376779E-5	3.4978089887183455E-4	0.0	2.0336098771618288E-5	6.507551606917853E-5
22-23	1.057477136124151E-4	4.1078919518668946E-4	0.0	3.2537758034589265E-5	7.72771753321495E-5
24-25	1.057477136124151E-4	4.3925973346695504E-4	0.0	6.914273582350218E-5	8.134439508647315E-5
26-27	1.057477136124151E-4	4.4739417297560237E-4	0.0	1.830248889445646E-4	8.134439508647315E-5
28-29	1.057477136124151E-4	4.5146139272992606E-4	0.0	0.001224233146051421	8.541161484079682E-5
30-31	1.0981493336673876E-4	4.962008100274863E-4	0.0	0.003359523517071341	8.947883459512047E-5
32-33	1.1388215312106242E-4	5.043352495361336E-4	0.0	0.0076301042591111826	8.947883459512047E-5
34-35	1.1388215312106242E-4	5.938140841312541E-4	0.0	0.013389287431233483	1.057477136124151E-4
36-37	1.1388215312106242E-4	6.954945779893455E-4	0.0	0.021885709498015604	1.057477136124151E-4
38-39	1.1388215312106242E-4	7.890406323387896E-4	0.0	0.03488047661307969	1.057477136124151E-4
40-41	1.1388215312106242E-4	8.663178076709392E-4	0.0	0.04876596485434066	1.1794937287538609E-4
42-43	1.1388215312106242E-4	8.907211261968811E-4	0.0	0.061707858112598546	1.2201659262970974E-4
44-45	1.1388215312106242E-4	9.88334400300649E-4	0.0	0.07599600110953755	1.3015103213835706E-4
46-47	1.1388215312106242E-4	0.001081880454650093	0.0	0.08890128939000652	1.54554350664299E-4
48-49	1.1388215312106242E-4	0.0010900148941587404	0.0	0.09943132133395047	1.7082322968159364E-4
50-51	1.1388215312106242E-4	0.0011957626077711554	0.0	0.10857849856142437	1.7082322968159364E-4
52-53	1.1388215312106242E-4	0.0012038970472798027	0.0	0.11823814547794306	1.8709210869888825E-4
54-55	1.1388215312106242E-4	0.0012323675855600684	0.0	0.1273568521671367	1.9522654820753558E-4
56-57	1.1388215312106242E-4	0.0013015103213835704	0.0	0.1336000344900235	1.9929376796185924E-4
58-59	1.1388215312106242E-4	0.0013259136399095125	0.0	0.13857017702980703	2.0742820747050655E-4
60-61	1.3015103213835706E-4	0.0013584513979441018	0.0	0.14332068970285705	2.1556264697915388E-4
62-63	1.3828547164700437E-4	0.0013828547164700437	0.0	0.147843438069665	2.1962986673347752E-4
64-65	1.4235269140132803E-4	0.0015292746276256953	0.0	0.15192692670300592	2.1962986673347752E-4
66-67	1.7082322968159364E-4	0.0015414762868886664	0.0	0.15655135556367192	2.1962986673347752E-4
68-69	1.7082322968159364E-4	0.0015496107263973137	0.0	0.16151336366394678	2.2776430624212485E-4
70-71	1.8709210869888825E-4	0.001557745165905961	0.0	0.16517386144283808	2.2776430624212485E-4
72-73	1.9522654820753558E-4	0.0015618123856602846	0.0	0.170249751696234	2.3589874575077215E-4
74-75	1.9522654820753558E-4	0.0015780812646775792	0.0	0.1747887689420592	2.3589874575077215E-4
76-77	2.033609877161829E-4	0.0016350223412381105	0.0	0.17881938371859396	2.3589874575077215E-4
78-79	2.033609877161829E-4	0.001687896198044318	0.0	0.18107262346248926	2.3996596550509582E-4
80-81	2.033609877161829E-4	0.0016919634177986417	0.0	0.18195521014917748	2.4403318525941949E-4
82-83	2.033609877161829E-4	0.001700097857307289	0.0	0.18378952625837747	2.4403318525941949E-4
84-85	2.033609877161829E-4	0.0017163667363245838	0.0	0.18521712039214505	2.4403318525941949E-4
86-87	2.033609877161829E-4	0.0017326356153418783	0.0	0.18604276600227276	2.521676247680668E-4
88	2.033609877161829E-4	0.001773307812885115	0.0	0.18612411039735924	2.684365037853614E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14660	0.0	48.842533	1
GTATCAA	27020	0.0	37.075768	1
TCAACGC	33220	0.0	29.400928	4
ATCAACG	33430	0.0	29.254152	3
CAACGCA	33955	0.0	28.69091	5
TATCAAC	34760	0.0	28.371279	2
AACGCAG	34885	0.0	28.02064	6
ACGCAGA	40090	0.0	24.136868	7
CGCAGAG	40705	0.0	23.802738	8
GTACATG	35700	0.0	22.702024	1
TACATGG	36050	0.0	22.25384	2
ACATGGG	36375	0.0	21.501766	3
GCAGAGT	47820	0.0	20.039042	9
CATGGGG	27980	0.0	19.485725	4
GAGTACT	27945	0.0	18.695368	12-13
AGAGTAC	42695	0.0	18.319078	10-11
GTGGTAT	6825	0.0	17.746185	1
CAGAGTA	46505	0.0	17.718761	10-11
TGGTATC	6765	0.0	16.568398	2
GTACTTT	31875	0.0	16.378847	14-15
>>END_MODULE