FastQCFastQC Report
Fri 27 May 2016
SRR2139341_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139341_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24070499
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA319150.051.3943331
GTATCAA567450.034.5121041
TCAACGC710600.026.9517464
ATCAACG715800.026.7907583
CAACGCA727050.026.3419445
TATCAAC745900.025.859022
AACGCAG743900.025.767646
ACGCAGA854800.022.4004127
CGCAGAG864550.022.1285488
GTACATG709900.021.4746781
TACATGG719450.020.8249822
ACATGGG717900.020.258783
GCAGAGT1000550.018.9298279
GAGTACT608850.018.83332412-13
GTGGTAT147100.018.7116851
AGAGTAC907600.018.23590510-11
CATGGGG528350.018.1596874
CAGAGTA981950.017.9309610-11
TGGTATC145150.017.4138892
GTACTTT685700.016.72675514-15
Produced by FastQC (version 0.11.5)