##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139341_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24070499
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.636632501885398	31.0	31.0	34.0	30.0	34.0
2	31.718936030366468	31.0	31.0	34.0	30.0	34.0
3	31.801955455929683	31.0	31.0	34.0	30.0	34.0
4	35.571472199226115	37.0	35.0	37.0	33.0	37.0
5	35.42583957233292	37.0	35.0	37.0	33.0	37.0
6	35.457316235945086	37.0	35.0	37.0	33.0	37.0
7	35.36247599187703	37.0	35.0	37.0	32.0	37.0
8	35.33716392834233	37.0	35.0	37.0	32.0	37.0
9	36.945372009113726	39.0	37.0	39.0	33.0	39.0
10-11	36.835315815430334	39.0	37.0	39.0	32.5	39.0
12-13	36.79284108318652	39.0	37.0	39.0	32.0	39.0
14-15	37.91498634905741	40.0	37.0	41.0	32.5	41.0
16-17	37.87312880385238	40.0	37.0	41.0	32.0	41.0
18-19	37.894374520445126	40.0	37.0	41.0	32.0	41.0
20-21	37.85991457446025	40.0	37.0	41.0	32.0	41.0
22-23	37.775860184822825	40.0	37.0	41.0	32.0	41.0
24-25	37.67121753088817	39.5	36.5	41.0	32.0	41.0
26-27	37.44958226204295	39.0	36.0	41.0	32.0	41.0
28-29	37.29907285564427	39.0	36.0	41.0	31.0	41.0
30-31	37.11606368305163	39.0	36.0	40.5	31.0	41.0
32-33	36.92191916563534	39.0	35.5	40.0	30.0	41.0
34-35	36.774883094947334	38.5	35.0	40.0	30.0	41.0
36-37	36.59671932848997	38.0	35.0	40.0	30.0	41.0
38-39	36.442350867113916	38.0	35.0	40.0	30.0	41.0
40-41	36.28880653519976	38.0	35.0	40.0	30.0	41.0
42-43	36.08343571204487	38.0	34.5	40.0	29.5	41.0
44-45	36.165939182055624	38.0	34.5	40.0	29.5	41.0
46-47	36.63184798170268	38.5	35.0	40.0	30.0	41.0
48-49	36.729078808589705	39.0	35.0	40.0	30.0	41.0
50-51	36.68474311544756	39.0	35.0	40.0	30.0	41.0
52-53	36.562449137713415	39.0	35.0	40.0	30.0	41.0
54-55	36.401744352844574	38.0	35.0	40.0	29.5	41.0
56-57	36.2254262515561	38.0	34.0	40.0	29.0	41.0
58-59	36.0501857368621	38.0	34.0	40.0	29.0	41.0
60-61	35.85038252461143	38.0	34.0	40.0	28.0	41.0
62-63	35.62750408291194	37.5	34.0	40.0	28.0	41.0
64-65	35.35060504249823	37.0	33.5	40.0	28.0	41.0
66-67	35.077690583111206	36.5	33.0	39.0	28.0	41.0
68-69	34.79128785111163	36.0	33.0	39.0	28.0	41.0
70-71	34.480980042780196	35.5	32.5	39.0	27.5	40.0
72-73	34.17273783866122	35.0	32.0	38.0	27.0	40.0
74-75	33.85505275451256	35.0	32.0	37.5	26.0	40.0
76-77	32.04200398544546	33.5	30.0	35.5	25.0	38.5
78-79	33.1675671226899	34.5	31.5	36.0	26.0	39.0
80-81	33.24089429200025	35.0	32.0	36.0	26.0	39.0
82-83	33.039667100499095	34.5	32.0	36.0	26.0	37.5
84-85	32.82103794613768	34.0	31.5	35.0	26.0	37.0
86-87	32.61947225105896	34.0	31.0	35.0	26.0	37.0
88-89	32.46179411796609	34.0	31.0	35.0	26.0	36.0
90-91	32.3315957727158	34.0	31.0	35.0	26.0	36.0
92-93	32.26610584246059	34.0	31.0	35.0	26.0	36.0
94-95	32.2803701520002	34.0	31.0	35.0	26.0	35.0
96-97	32.37881231329982	34.0	31.0	35.0	27.0	35.0
98-99	32.53708661707887	34.0	31.0	35.0	27.0	35.0
100	30.937564733928188	33.0	29.0	34.0	24.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	59.0
20	263.0
21	1826.0
22	8800.0
23	29907.0
24	75660.0
25	152345.0
26	263178.0
27	395732.0
28	542455.0
29	691072.0
30	843432.0
31	1007281.0
32	1200960.0
33	1454026.0
34	1810466.0
35	2312226.0
36	3117577.0
37	4193706.0
38	4069497.0
39	1835923.0
40	64106.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.77742218804853	21.088366302667843	14.635654208913577	23.498557300370052
2	14.724149630038088	22.04332470374511	40.991699089325806	22.240826576890996
3	17.845638258086723	27.221435136074046	31.280412229111338	23.652514376727893
4	12.571953743044546	18.735727082350888	39.14352170264522	29.548797471959347
5	12.532590205130356	38.62725072712452	36.164430990815774	12.675728076929357
6	29.226818272442127	39.21298848021389	18.714996311459934	12.845196935884045
7	25.53319004866455	33.318326027155734	23.959192701372444	17.18929122280727
8	24.20894971890695	37.31318989273966	21.214790769397844	17.26306961895555
9	25.496409526034334	16.423186739917604	21.337334967588333	36.74306876645972
10-11	23.888841689466066	26.96486915800684	29.533226327971917	19.613062824555175
12-13	24.739639283570796	24.681825545633252	29.49925829037307	21.07927688042288
14-15	21.98094256763129	26.01962817737083	26.980380879435874	25.019048375562008
16-17	21.391384879596384	29.087714262585735	27.65518928812069	21.86571156969719
18-19	21.442072249456533	28.194234708065412	29.425097504782826	20.938595537695225
20-21	22.271619890241894	27.364391819920677	29.267123931515183	21.09686435832225
22-23	22.028414960929528	27.248842122620093	29.16380360275337	21.55893931369701
24-25	21.79276152726764	27.485756061397947	29.16680540329168	21.554677008042734
26-27	21.753728769976142	27.555100375994552	29.09518101886811	21.5959898351612
28-29	21.763894199307032	27.569963086803924	29.104760245295978	21.561382468593063
30-31	22.02280688190711	27.47898527141441	28.927515995015035	21.570691851663444
32-33	21.846810265091424	27.68229786462567	28.862242079820273	21.608649790462632
34-35	21.947071107505035	27.65972504595069	28.916632799399554	21.476571047144724
36-37	21.939078361476643	27.61417118684284	28.803358125671114	21.643392326009405
38-39	21.78879380705034	27.727259043998586	28.84586651222169	21.638080636729384
40-41	22.03850517315069	27.57299319668199	28.72326400593419	21.66523762423313
42-43	21.913715556714784	27.7138026263903	28.45210682644029	21.920374990454633
44-45	22.008689282946104	27.82296153632471	28.26161694954018	21.90673223118901
46-47	22.129561943353593	27.81618214348328	27.971903774583573	22.082352138579555
48-49	22.012730353209157	27.979776520073962	27.916846232234764	22.090646894482116
50-51	22.02375117882582	28.057414354698924	27.827884256142806	22.090950210332455
52-53	22.171372988002492	27.93023796412483	27.73506404990386	22.163324997968818
54-55	22.145850381262637	27.82566121617615	27.814902546316382	22.213585856244826
56-57	22.1334073629376	27.93429505228972	27.80188447133758	22.1304131134351
58-59	22.108509956727083	27.970702626618436	27.79415827576981	22.126629140884667
60-61	22.167788434996098	27.995975337624817	27.77858751766027	22.057648709718812
62-63	22.154765277986833	28.051276780264402	27.726710134640097	22.06724780710867
64-65	22.11415062284918	28.01641262629446	27.655766859798902	22.213669891057453
66-67	22.132869403978653	28.073909035982393	27.540542313060367	22.25267924697859
68-69	22.08668640434046	28.016462321999143	27.62268523874833	22.274166034912074
70-71	22.10763632390717	27.780486260526683	27.708266031661687	22.40361138390446
72-73	22.193948424527775	27.80209451837156	27.585580856235715	22.418376200864948
74-75	22.031764684835096	27.95766431445761	27.655684871639053	22.354886129068248
76-77	22.23200188728083	27.851764791429233	27.6030056434572	22.313227677832735
78-79	22.154220608120916	27.88382739847301	27.51726168827464	22.444690305131438
80-81	22.194374814128086	27.894643319008562	27.517942902005206	22.393038964858146
82-83	22.21766603808034	27.816784265534267	27.65735914242769	22.3081905539577
84-85	22.274399618216222	27.813326956768442	27.64239513214189	22.269878292873447
86-87	22.144193498400767	27.845209847960696	27.751517513689546	22.259079139948994
88-89	22.19233319364074	27.7776112747279	27.716828848506015	22.31322668312535
90-91	22.248794382701064	27.76682564746189	27.800877052907403	22.18350291692964
92-93	22.300890363278377	27.69457708706445	27.79848823038071	22.20604431927647
94-95	22.058978674969325	27.666529286796482	27.991872563073972	22.282619475160224
96-97	22.075211032909618	27.81373529765338	28.090173003546848	22.020880665890154
98-99	23.102065464988055	29.822527038435236	28.300873717952385	18.774533778624328
100	22.63071269231779	30.30257174291635	22.080026475793588	24.98668908897227
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5942.0
1	5256.0
2	7418.0
3	10810.5
4	13996.0
5	22496.5
6	30439.0
7	37001.5
8	42139.5
9	42057.0
10	37761.0
11	32854.5
12	34326.5
13	44088.5
14	67168.0
15	103615.5
16	134846.0
17	151708.0
18	158283.5
19	155369.5
20	146794.0
21	137005.5
22	128605.0
23	131468.0
24	150560.5
25	184804.0
26	231171.5
27	282500.5
28	342031.5
29	411900.0
30	477534.5
31	538504.5
32	601019.0
33	656799.5
34	706228.0
35	748607.5
36	791739.0
37	832369.0
38	855214.5
39	866058.5
40	881513.0
41	897973.0
42	905324.5
43	912308.5
44	922331.0
45	936822.5
46	945953.0
47	945806.0
48	944675.0
49	932965.0
50	916862.0
51	900902.5
52	873533.5
53	835582.5
54	793290.0
55	746975.0
56	697322.0
57	640030.5
58	576301.5
59	506843.0
60	428176.0
61	350289.5
62	280394.0
63	219367.5
64	165258.0
65	122962.0
66	92394.5
67	70624.5
68	54351.5
69	39596.5
70	28323.0
71	20475.0
72	14289.0
73	9613.5
74	6516.0
75	4502.0
76	3170.5
77	2186.0
78	1480.5
79	1059.5
80	769.5
81	567.5
82	430.5
83	292.5
84	195.5
85	137.0
86	101.5
87	75.0
88	47.0
89	31.0
90	24.0
91	20.0
92	15.0
93	8.5
94	4.0
95	2.0
96	1.5
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.1276541878089025
3	4.154463104400121E-6
4	0.0
5	0.0
6	0.0
7	4.154463104400121E-6
8	0.0
9	0.0
10-11	2.243410076376065E-4
12-13	3.967512264702115E-4
14-15	8.516649364020247E-4
16-17	2.6380840712940764E-4
18-19	0.0011196278066358326
20-21	8.1017557252511E-5
22-23	0.0017951506622786591
24-25	1.3925604391495464E-4
26-27	0.005604622634519106
28-29	0.0026597922760506413
30-31	0.004791964140850646
32-33	0.017480772612951914
34-35	0.0038980524457689477
36-37	0.002203733520666133
38-39	0.001221072764998017
40-41	7.024003260845478E-4
42-43	0.0013533851799632207
44-45	4.1282539875674374E-5
46-47	2.5478381878509676E-4
48-49	1.4859811869458815E-4
50-51	5.193062972178411E-4
52-53	2.933927081936662E-5
54-55	0.0
56-57	0.0
58-59	1.646563364837115E-4
60-61	0.0
62-63	2.6045450162930863E-5
64-65	6.700823957245297E-5
66-67	0.0
68-69	1.9609519558926996E-4
70-71	9.932289102118581E-5
72-73	9.634699730809509E-4
74-75	1.4781030250041004E-4
76-77	0.0022335560631996693
78-79	0.0025871294989894236
80-81	0.0027920649196497066
82-83	8.354195015908813E-5
84-85	0.0
86-87	0.0017776690218972936
88-89	4.504060325401079E-4
90-91	6.044933931764439E-4
92-93	7.105232820420392E-5
94-95	3.650482374740998E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1014.0
20-21	3290.0
22-23	6649.0
24-25	13015.0
26-27	15825.0
28-29	29096.0
30-31	58322.0
32-33	74510.0
34-35	104241.0
36-37	139698.0
38-39	162106.0
40-41	176422.0
42-43	206748.0
44-45	257444.0
46-47	224889.0
48-49	210256.0
50-51	188262.0
52-53	178778.0
54-55	192433.0
56-57	212684.0
58-59	221101.0
60-61	221778.0
62-63	222230.0
64-65	233771.0
66-67	253028.0
68-69	260455.0
70-71	262675.0
72-73	255529.0
74-75	262469.0
76-77	241445.0
78-79	270382.0
80-81	285552.0
82-83	283780.0
84-85	291036.0
86-87	313535.0
88-89	351317.0
90-91	391141.0
92-93	435276.0
94-95	527249.0
96-97	1063138.0
98-99	1751329.0
100-101	1.3216601E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.95167804516025
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.98162505537539	71.22260185372753
2	7.770766105518501	12.581131118763548
3	2.1726168598655855	5.276309416659776
4	0.8673105772318754	2.808409864529475
5	0.42714769906199246	1.7289161506098705
6	0.249854561492133	1.2135687612015504
7	0.1402251250866409	0.7946021425899255
8	0.09840105060062272	0.6372584134021706
9	0.06398307053122369	0.46615832333859614
>10	0.22180054593679832	2.765906334951607
>50	0.0048689564238780444	0.2577370212043875
>100	0.0013131958355413363	0.17145434890293998
>500	4.3666028988974705E-5	0.027396006732685742
>1k	4.353101081028752E-5	0.0485502433859891
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.30892620880024E-6
2	0.0	4.154463104400121E-5	0.0	0.0	1.2463389313200363E-5
3	0.0	4.154463104400121E-5	0.0	0.0	1.2463389313200363E-5
4	0.0	1.0386157761000301E-4	0.0	0.0	1.661785241760048E-5
5	0.0	1.1632496692320338E-4	0.0	0.0	1.661785241760048E-5
6	0.0	1.1632496692320338E-4	0.0	8.30892620880024E-6	3.323570483520096E-5
7	0.0	1.3294281934080385E-4	0.0	8.30892620880024E-6	3.323570483520096E-5
8	0.0	1.4540620865400423E-4	0.0	8.30892620880024E-6	3.323570483520096E-5
9	0.0	2.4511332315960713E-4	0.0	3.739016793960109E-5	4.5699094148401326E-5
10-11	0.0	2.4511332315960713E-4	0.0	5.6085251909401626E-5	4.5699094148401326E-5
12-13	0.0	2.513450178162073E-4	0.0	7.893479898360229E-5	5.193078880500151E-5
14-15	2.07723155220006E-6	3.4274320611300997E-4	0.0	8.932095674460259E-5	7.685756743140224E-5
16-17	4.15446310440012E-6	3.5728382697841035E-4	0.0	1.100932722666032E-4	9.555265140120278E-5
18-19	4.15446310440012E-6	3.9051953181361136E-4	0.0	1.2671112468420366E-4	1.1217050381880326E-4
20-21	4.15446310440012E-6	4.13369078887812E-4	0.0	2.0980038677220606E-4	1.1424773537100331E-4
22-23	4.15446310440012E-6	4.6737709924501357E-4	0.0	5.608525190940163E-4	1.2671112468420366E-4
24-25	4.15446310440012E-6	5.068444987368147E-4	0.0	0.001441598697226842	1.412517455496041E-4
26-27	4.15446310440012E-6	5.317712773632154E-4	0.0	0.0037120127837815082	1.4332897710180416E-4
28-29	4.15446310440012E-6	5.338485089154154E-4	0.0	0.012783282972239171	1.4540620865400423E-4
30-31	4.15446310440012E-6	6.086288447946177E-4	0.0	0.015230261740730843	1.5163790331060442E-4
32-33	4.15446310440012E-6	6.127833078990178E-4	0.0	0.01963191539984277	1.7033298728040495E-4
34-35	8.30892620880024E-6	6.958725699870202E-4	0.0	0.024881079532252322	1.827963765936053E-4
36-37	8.30892620880024E-6	7.851935267316228E-4	0.0	0.03354105787337437	1.8487360814580536E-4
38-39	8.30892620880024E-6	8.018113791492232E-4	0.0	0.06070501488149456	2.0149146056340587E-4
40-41	8.30892620880024E-6	8.890551043416259E-4	0.0	0.08656239324328091	2.1187761832440615E-4
42-43	8.30892620880024E-6	9.28522503833427E-4	0.0	0.1030161443682576	2.2226377608540644E-4
44-45	8.30892620880024E-6	0.0010510791654132305	0.0	0.12528406660784225	2.3680439695080688E-4
46-47	8.30892620880024E-6	0.001144554585262233	0.0	0.1474294321858471	2.534222493684073E-4
48-49	8.30892620880024E-6	0.0011487090483666333	0.0	0.16779253309206427	2.7004010178600783E-4
50-51	8.30892620880024E-6	0.0012795746361552373	0.0	0.1888951284308647	2.7004010178600783E-4
52-53	1.2463389313200363E-5	0.0012878835623640374	0.0	0.2446459460603621	2.970441119646086E-4
54-55	1.2463389313200363E-5	0.0013169648040948384	0.0	0.31318212389365085	3.2820258524760953E-4
56-57	1.2463389313200363E-5	0.0013543549720344394	0.0	0.3798882607294515	3.3235704835200965E-4
58-59	1.2463389313200363E-5	0.0013564322035866394	0.0	0.4169232220736263	3.3858874300860984E-4
60-61	1.2463389313200363E-5	0.0013875906768696404	0.0	0.44318358335653946	3.4482043766521003E-4
62-63	1.2463389313200363E-5	0.0014249808448092414	0.0	0.462740718420503	3.614382900828105E-4
64-65	1.2463389313200363E-5	0.001580773211224246	0.0	0.46923206702112824	3.6351552163501054E-4
66-67	1.2463389313200363E-5	0.0015953138320896464	0.0	0.475459607214624	3.718244478438108E-4
68-69	1.2463389313200363E-5	0.0016347812315814475	0.0	0.4812467743190534	3.78056142500411E-4
70-71	1.2463389313200363E-5	0.0016617852417600482	0.0	0.48679921425808415	3.988284580224116E-4
72-73	1.2463389313200363E-5	0.0016784030941776487	0.0	0.49115724605459987	4.5283647837961313E-4
74-75	1.2463389313200363E-5	0.00172410218832605	0.0	0.49459298704193877	4.5283647837961313E-4
76-77	1.2463389313200363E-5	0.001792650829548652	0.0	0.4978646267366539	4.590681730362133E-4
78-79	1.661785241760048E-5	0.001823809302831653	0.0	0.5001059595814777	4.652998676928135E-4
80-81	1.661785241760048E-5	0.0018362726921448535	0.0	0.5008080638461213	4.6737709924501357E-4
82-83	1.661785241760048E-5	0.0018445816183536535	0.0	0.5013502212812455	4.777632570060139E-4
84-85	1.661785241760048E-5	0.001859122239219054	0.0	0.5015849484466441	4.81917720110414E-4
86-87	1.661785241760048E-5	0.0018923579440542548	0.0	0.501717891265985	4.8399495166261407E-4
88	1.661785241760048E-5	0.001969215511485657	0.0	0.5017303546552981	4.943811094236144E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	31915	0.0	51.394333	1
GTATCAA	56745	0.0	34.512104	1
TCAACGC	71060	0.0	26.951746	4
ATCAACG	71580	0.0	26.790758	3
CAACGCA	72705	0.0	26.341944	5
TATCAAC	74590	0.0	25.85902	2
AACGCAG	74390	0.0	25.76764	6
ACGCAGA	85480	0.0	22.400412	7
CGCAGAG	86455	0.0	22.128548	8
GTACATG	70990	0.0	21.474678	1
TACATGG	71945	0.0	20.824982	2
ACATGGG	71790	0.0	20.25878	3
GCAGAGT	100055	0.0	18.929827	9
GAGTACT	60885	0.0	18.833324	12-13
GTGGTAT	14710	0.0	18.711685	1
AGAGTAC	90760	0.0	18.235905	10-11
CATGGGG	52835	0.0	18.159687	4
CAGAGTA	98195	0.0	17.93096	10-11
TGGTATC	14515	0.0	17.413889	2
GTACTTT	68570	0.0	16.726755	14-15
>>END_MODULE